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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9761eca8e6154f2a9f3f74c0f71b9072Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""#####################K1313_C2_Cuphea_sp_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z((()*+,+*(%#""$&()(&$! 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 3PkxbJ3         1G]oxwm[F5+,5EVdlkcUD3%              "0=IOPJA7-'%(.6=CFE@8.#      )6AHIE<1(! %-8BIKIC:/$         #+38:95/(!  #-6=@@<5-$   !(-01.)#"'-25541,&   !##!  #$#     $.8?EGFB?<;<?ACB?;5/(#  %.7>CEEDB@=<;;;<;:9740+&"!(19@EHHFB=6/(" "$'+07?FMQQNG>4+# F @"AAGOAeKB.bcpKB_3730_POP7_BDTv3.mobtermACTTCTCTGACTAGCTGCTGTCGAGCTCCATCTACAAATGGATAAGACGTTGATCTTAGTGTAAGTGTATATGAGTTTTTGATTTGAAAGTAAAGGAGCAATAAACAATATTCTTGATAGAACAAGAATTTTTTTATTGCTCCTTTATTTAGTGTTCTTTTTTTTTATTTGTTGTATTACATCAATTTTTCTATTTTTTATGTCAACATCAACAAATAGAATATAAGAAAACTCTTCTTAAATTGTTAAATTGAATTGGGTTAGGATGAATATCCTACTTTACTTCCTTTTATTTTCAATTTTCAGTTGATTGATTATTAATTAATTTTGTAGTTAATTTTAATTCCAGTTTTTTTATTTATAATTTAGATTTTATAGAAATTTGAAATATAAAGTTATTGTCCATTTTTATCCTTTAATTAATTTAATTATTTAATTAATAATACGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCACGACCCGAGGACTTCTCTGACTAGCTGCTGTCGAGCTCCATCTACAAATGGATAAGACGTTGATCTTAGTGTAAGTGTATATGAGTTTTTGATTTGAAAGTAAAGGAGCAATAAACAATATTCTTGATAGAACAAGAATTTTTTTATTGCTCCTTTATTTAGTGTTCTTTTTTTTTATTTGTTGTATTACATCAATTTTTCTATTTTTTATGTCAACATCAACAAATAGAATATAAGAAAACTCTTCTTAAATTGTTAAATTGAATTGGGTTAGGATGAATATCCTACTTTACTTCCTTTTATTTTCAATTTTCAGTTGATTGATTATTAATTAATTTTGTAGTTAATTTTAATTCCAGTTTTTTTATTTATAATTTAGATTTTATAGAAATTTGAAATATAAAGTTATTGTCCATTTTTATCCTTTAATTAATTTAATTATTTAATTAATAATACGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCACGACCCGAGG-5A[at}!-:MZgs%-9DR_iw#.;FT_jt !-;FQ\hs} !+7CNZgt &0<HUamz  ,7DP\ht(4@MYdn{  % / ; H U a m y    ) 5 A M Y f s   & 2 > K W b m y    + 7 E Q ] i t    ' 4 A M X d p |  !-9FR^iu)4AMYdq~&2>KWcn{ ".9GS_kx ,9DP\gs~(5ANZfq}%2>KVao{ $1=JVcq{'3>KWdp{&3>JVbnw-5A[at}!-:MZgs%-9DR_iw#.;FT_jt !-;FQ\hs} !+7CNZgt &0<HUamz  ,7DP\ht(4@MYdn{  % / ; H U a m y    ) 5 A M Y f s   & 2 > K W b m y    + 7 E Q ] i t    ' 4 A M X d p |  !-9FR^iu)4AMYdq~&2>KWcn{ ".9GS_kx ,9DP\gs~(5ANZfq}%2>KVao{ $1=JVcq{'3>KWdp{&3>JVbnw  !%&* 5.6:'5) 64-:::64///>>%.>11>>18>1>>18>8>/& &3>>#>;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>38>>>1+;>;;>>>>>;>>>>>>>>>>;>>>;;11>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>88>>>>>>6*#& 3>>>>88>8>>88>8>>>>8>>8>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>;>18>>>>;>;+>>>;>>>>;>>>>>>>;>>;8>>;>;>>>>3>>>>>>;>>>>;+>>;,>>;;>>>>>;3>8>>8>>>>88/>>,8#'8$&338>>>>>>8...!&!>8888888(42/*44(.6444464> *"(.44*46.0""!*) "444,(4')'%4/!/%'.'./'/* -6%2"!2522*21(*2.2'4'0#&.23)''2,#%2).).154,   !%&* 5.6:'5) 64-:::64///>>%.>11>>18>1>>18>8>/& &3>>#>;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>38>>>1+;>;;>>>>>;>>>>>>>>>>;>>>;;11>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>88>>>>>>6*#& 3>>>>88>8>>88>8>>>>8>>8>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>;>18>>>>;>;+>>>;>>>>;>>>>>>>;>>;8>>;>;>>>>3>>>>>>;>>>>;+>>;,>>;;>>>>>;3>8>>8>>>>88/>>,8#'8$&338>>>>>>8...!&!>8888888(42/*44(.6444464> *"(.44*46.0""!*) "444,(4')'%4/!/%'.'./'/* -6%2"!2522*21(*2.2'4'0#&.23)''2,#%2).).154, FN\ =+S]?[PPYO+C ;E>6>7PMS4C *;;K.-V5HJ*9H>$>X6-BM2!'%<?Z,.QG.=G"7\/ "R`LXb%ZWD 0 \'3'A7)4JF2K): 3@3X+:#'F1 Z; 4P"J5T"L/K[[\YU;B5X cSQ 7,HY"[$V\$N[86PL(6.R/0DP.')VV#XJHL<= aK< 'Ob0M3>'HP^)4 M!N5'U!;"4&T&OCEL,M2N] OaPG*Q8%JZ^[:;_Y)6.@B2^B\1LF6D#A_44C@:UJ4K>9 S)AU7-90K):bG`'Q4J"@G&V-\M2; #N.5ZXIJ/;LI?MD!COOU:` -[`-3F7DBIC&1:5VO:`M/*0Y4;Q-MV&GQKDGP32%2DG:B$S&>1DPOJ&>)Z'IJ+  @oCe \BZ^(+D"eC2>r *BgX%'w4\J|aP=mvA;0sE#l3z*>UIL ,zw2UN~/%a;} QJ] ;R*=l"9-SfKZ<O1h_dT3I14:p _LJEJF)bSYXI+7 c*:Bzv-FRCEce&&jRTRN&&(?vZ7R=,Gt>>`Vty~yKV=2'"C5?ql/crt) <UY3e0Z>}% H1D^!:x|>_.~dhX`W]UnW~mUG58;A?&&2K*1+JW3+1%-+$ !!$   $! /             %       #-! !#"  $& #)6+L' "%)#  !0 -&87"'-&".2/0#49!,.4  /@7*B&.)%044TKi,;>V@d2/>M<;4%D`GGGG7>Ed19?RGCI:?FMGSDDUDGGG9WGGKAGJwe=R ;Fpq`s]YgxiRR<PIXIIPbnPPIIbIXIIPGGSGRRPRRPRIPRRRIWpppp5pp>5E\s8Dp8~688|3hLMp^DNbp,{dqFpp(Nn8%dv>GpuM@mIXoN8u^uVm1E j8% =D,G!Lx&4wi,Y)P;A,A,wCsp$)p#pFppa8,,dx[Y5RN`'y=b8R$v*`1,A0%/.( ##R.!2<2n"L;a 3H3Af% 6$*! #$ $+2CUM%. %' ' . %)0&('$),'$:! 9+,3 F./% .$sqF`FG?a\ )k<0Q<4V/4 '.#')=07VOK6DRHb%)@R/95&2T18(F/%F-F C$/#&EB>#w3%b.+#Q8=D)=,))VO|/MXW*8$BuY?J^!P)%) lj"A!`NXV_+PQ6QlE<H<Hi;AZ1'\Nohsg7eQmKWrg8eBhK4[8YcC;cmLJ/2LXHxf}]@gI4|?Zh^+;ve ~~gFT}n]j$|j_ UCAACTCTAACAATATGTGGTCTAGAGCTACATATACTTAAATATGCCATGCCTATCCGAGTATGAGTGTACACCCCCAAAAGAGCCCGTTATCCATGAGCTCTTTATTATGATCCCCCCCCGGGGAGTAACAACATACAACAAAAATAAATAGAACTCCCCAACCGTAAAAGGAATAATACAGGGAGGAATGTCCCCCATACAACCTCATCCACTTCCATTATACGATTTGAGACAAGCTTTCCAAATTCCAATTGGTTAAGTTATAATTATAAACCGAAATACCGACGGATAACAACCAAAAACAGAAGAGACAACAATTAATGGGACCATAAATGAAACCCCGGTCCAACCCGATAAATGGTAAATCCGCCCTATCTTTCAAATCCATACCCCAATACCCTTCCCCCCTGAACAACGGCCTAACCCAATGGATTAGTTGTTACAACCCGCCCAAGGTGTCGAGTAGAGTTATGCCCTTTACTGTCTAGAGAACAAGAAAAGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:16:58 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtlASPtlB1PtkB1PtkBCTSxBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?:TDATA ?:DATA ?:S(DATA ?:bDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~wRLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMeP1RLaP1WDiP2AMqgP2BAuYP2RLmuPBASSPBASQPCON_PCON]PDMFTPDMFQPLOCYPLOCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%QSCAN@SMED SMLtSMPLTySPACAa&SPACQSPACAa&SVER4.0SVER xSVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHQphDYbigphQLcphTRphTR큅This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtlASPtlB1PtkB1PtkBCTSxBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?:TDATA ?:DATA ?:S(DATA ?:bDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab6FVoc%FWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~wRLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMeP1RLaP1WDiP2AMqgP2BAuYP2RLmuPBASSPBASQPCON_PCON]PDMFTPDMFQPLOCYPLOCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%QSCAN@SMED SMLtSMPLTySPACAa&SPACQSPACAa&SVER4.0SVER xSVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHQphDYbigphQLcphTRphTRr/)ecAAx{!9Y2]C0nD2,3D?x%ZH|x+1[n\PF7^%4G}UlL`QF_4}V3Er]mz]~C*/meqy{g_hD6E{wsUfGX.!6^q;,`h?X??-|D>){6ft[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}sP:gtqbg(*2K]?ZO~|Ue_v#a}_#S!KnMfgmw/U9kgQlxw+;?+RX, W,?Gc39EMh`+MTk0WC.%o,n`!3CS[jWcgnW6Ob#7!VkWgtKkq$1j#xFsB)E02|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wAEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtlASPtlAUDTIB1PtkB1PtkBCTSxBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?:TDATA ?:DATA ?:S(DATA ?:bDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab6FVoc%FWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~wRLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMeP1RLaP1WDiP2AMqgP2BAuYP2RLmuPBASSPBASQPCON_PCON]PDMFTPDMFQPLOCYPLOCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%QSCAN@SMED SMLtSMPLTySPACAa&SPACQSPACAa&SVER4.0SVER xSVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHQphDYbigphQLcphTRphTR