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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5390e2ce71d34e7a93c72149bf58cca0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""#####################"K1313_C10_Heterocentron_sp_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z 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Dt_5EvX/,Up@  !"##$$#####"  (MxoG&*R}K(,SyJ% *Ny]7,TnD" 8eZ/>jU. BnrH%%GqvG"!M([mZ)X( 8bqB!#I d7.QzlC&+PM% &Jw_7"*OM_LQ5<e&HH(rOKg/0nOOl ^IQv"V,+Nv\=4Dn1+Z=?` )) fGC\_HKh _KPnzVHTw sNAPvfG@RwY>;Ou|S:7JmnH+   +Hi^>&*GngC&  -JitT5 (FpiB*(>f^:*1MuoI1-<[pM/   3Qn~eG, ,Hev[>%   /Id{oT;*%-A[vhL2 8XyvW9! ,F`vv`F,!9Sjy|tcK3  3QpoO1&AesN.!8aa=+-BdzU5#$7X}lI+"8Ocork\H3!  .E[krnaN9& &=UhttkYD. %?\u~iP9,->\kJ4.8NjpS9'$.Ea}~cE**BZkspcO9$  /DWdhbTA-$8L\dd[L:(  /EYgkfXD. "7M_jkbR>* -?NXZSG6% !3DQWUK=,  ';N^fe[J6# 2G\inhYE0  &8JX^[P?, 2MjfI0   &6FPTPF7' $1<BC?5)  "0?KRRL@2#  3FV`b\O>,  (8ENQMC6(  .<GLKC8*  '5@GG@5)&5FVaeaUD2! !+3773+" *7AFD>3'   %'&#   !"#%%&'''''(()*++,,,,,,,,++++++*))('&%$$$$##"! ../013556667788888889;>BEILOPQSV]ep}(Kp'c/vD$OsgAN ?)9GQTN>%zEwI wkb\XWXZ[\\]^bhr 3_H=s+,U2e.wbRG>8420.-,,,,-/137=FRbxBz'[s?JiSE;6336>J\t0};r>TO0MtDeK9-&#""#######""""""""!  #/A[~c#Njwytomq{yR=yR9*#!!"####"#'2GlM*)>+'?O)   7[o-<+gK' 'MJTQ*  1_:pF!S  LP@  EJ$'\U%  ACltA$*5AMXcmwuh\QH@93-("   @v)DB#r> !$'*,,.5Hl2xJ~pmnpomhc^YTNHA:3-(#  /_bILih:>s*R)4dU+ #M(7'cTc lUWpmPFStaFAU~dF?Qvg@#=p' a6 "ErmE'  !#%(+.1468;?CGJLMLKJJJJKJIGEB@><:852.+'$!    7WzrS7# .Lk{_C*   .Ie}oV<'   ,ATafaTA.  4J]ileWD1   4Ngy{lV@.&(3CTageZI7%  )<MY]ZPB1"  ,BXjtum^K7% '6BJLG=0$!.>NZ_]SE4$ ,9DHG@6*  (5?EE@6*   +3895.%  +>O\a]RB2('/?SdmmbP;(  !/<FLMJC:0(##(0:DKNKE<1'  '5BMSTPG</$ /CXiuzvk[I8*    g@rA_pAX A)KB.bcpKB_3730_POP7_BDTv3.mobtermAAATTCTCTGACTAGCTGCTGTTGAGTTCCATCTACAAATGGCTAAGACTCTTTTATTAGTCAAAATTGAGACGAGTTTTTGATTTGAAAAAAAAGAGGGGGGGGAGGATCAATAATCAAAGCCTATTGATATAAAATCAATCAAATAATTATTGTTCCCCCCTTTTTTCTTTTTTTTTTTATTATTTTTAATCTGTTCAAGTATCTATTTTTGAATATAGATACAAAGAGTTTTCAAAAAAATGAAAATATATGAAGTAATAGACTATTACTTCCGAGACGTAGGGGCGGATGTAGCCAAGTGGCTCAAGGCAGTGGATTGTGAATCCACCATGAAGAGAAAAATTCTCTGACTAGCTGCTGTTGAGTTCCATCTACAAATGGCTAAGACTCTTTTATTAGTCAAAATTGAGACGAGTTTTTGATTTGAAAAAAAAGAGGGGGGGGAGGATCAATAATCAAAGCCTATTGATATAAAATCAATCAAATAATTATTGTTCCCCCCTTTTTTCTTTTTTTTTTTATTATTTTTAATCTGTTCAAGTATCTATTTTTGAATATAGATACAAAGAGTTTTCAAAAAAATGAAAATATATGAAGTAATAGACTATTACTTCCGAGACGTAGGGGCGGATGTAGCCAAGTGGCTCAAGGCAGTGGATTGTGAATCCACCATGAAGAGAA#.9C_cw (4AScpz".;FQ^lv ",8DN[iw !.;HT^jt+5BOXcoz !.:GR]hs #.;FQ^lx  ,8DP\ht    # / < I U a n y  " . : F Q \ g r    ' 2 = H T ` l x    ( 5 @ L X c n {  # . < F R ^ i t '4@MXep} ".9FR]ht!#.9C_cw (4AScpz".;FQ^lv ",8DN[iw !.;HT^jt+5BOXcoz !.:GR]hs #.;FQ^lx  ,8DP\ht    # / < I U a n y  " . : F Q \ g r    ' 2 = H T ` l x    ( 5 @ L X c n {  # . < F R ^ i t '4@MXep} ".9FR]ht!  '!%+'$% 3:"5'6/&$4.4::">>>>>4//)>>/>>>6>>>>/*>>1>//>>>>>>>>>>>>>>>>>>>>>;;>+>>>;>;+'>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>6>>6>>>>>1>>8*  >>8>68>>>>>>>>>>>6>6>>>8>>>>>>>>>>8>>>>>>6>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>/1*>>>>>6:::-':::::6:::1"+:::4::66:::0!)::/:::6::".(    '!%+'$% 3:"5'6/&$4.4::">>>>>4//)>>/>>>6>>>>/*>>1>//>>>>>>>>>>>>>>>>>>>>>;;>+>>>;>;+'>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>6>>6>>>>>1>>8*  >>8>68>>>>>>>>>>>6>6>>>8>>>>>>>>>>8>>>>>>6>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>/1*>>>>>6:::-':::::6:::1"+:::4::66:::0!)::/:::6::".(   "#/=$4c*<S:B:D-._`L_=F5$ B5D 55)6,X 0KR)$#b`[UJ1DS=\(`LUOF*QVW )LU.)23 F  JCc\Ib6( .,V7V<QG:_[&25+,* O8$RaMR!'Yc IV *KNC:84KK=+ [YY\B#QAE 'S9 ;]3C$2GTJ !A+< L; /MS7TV EV6V[^(_/@=5%]2)]A@>IN_UQ*7G;G'#;OJa ,>N)`6 ]1W^O"^8^2M=P5 1b;;/@BGJ?@X9TO-%5aM2:j<TfPdrNNY. t#aD8:]Zx{hadp2w{#I3yB3VC6^ePk13U@h7j? )=R/u|W3\FKnN\| FVtAsm*w.'\6Tovw.} sbL&ElmedG-y_?lV^"^ M)3aj  =Rqo OjN|4 u~p=+ 8hhpSlOS94.~}mNb\G`^GiYSK7*23G,5G?<<Vk;0*.'5. 1: .!/(    $!:      C"           & '&&(&%$"!  '  & #$ ,#"5$ ##&.# #'+7"$&.)$,0',1+,8&&6 (&&(H'O^ppp8Gmp7P8p7XCppKBA|b,=Jp{Byd<.3Jep4, ^gq80,pp:YN8S (8pd6pr,&D1NfU Mt  lF]";y!58".v5 $aUc)QI2N)<E:Ql)   P& 9s%ZX+'9610 "9"/)'      J+$ 0so9Y_+B$V3#B"37-5&*@6546(:("+@#)%+%M('%@&-# & (: ,#1 @+,L?*AI->'B95B&F2<+03(r8@>0+ME.#G<O:/=Z:c8"Bc2;bITATTTACTCTAACAATATGTGGTGGTGAGCTACATATACGGGACGATGACACTCCAATAATTGACGGTAATGTCACCCCCAAAAGAGGCCGCTGGGGAGCCCCCCTAGACTATGGGGCCTCCTTAAAGGGTTATATGGGGACGAACGGGGGGGGGGCGCTGGGGGCCAGGGTGGAAAAAAAAATAATAGGCATTCTCCCCACGAATATCCCCCCGTGTATATAGACACGGAGAGCCCTCGGGGGTGTGGGGATATATGGAGTTATAGAATACTTCCTAAGACAAATAACCGCAGATGTAGACTTGTCTCTCGAAGCAGTATACTGAGCATTCAGCAACGCGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:18:00 -04:00AEPt@AEPt@APFN TAPXV2APrN 1 APrV1APrX}}1ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTSVBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA DATA iDATA &;DATA <yTDATA <yΞDATA <yHHDATA <yDCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 09EVNT 0EEVNT0QEVNT0dFWO_GATCGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANELLAST~~U9LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;OfScP1AMVI3P1RLVFP1WDVKP2AMVQ7P2BAVVSP2RLVNPBASVV=-PBASVV;PCONVVE1PCONVVCPDMFTPDMF;PLOCVA/PLOCV>PSZE`PTYPJPXLBRGNmJRMXV1RMdNBRMdV1RMdXBRPrNJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0wRunN""JS/N%;SCAN@SMED SMLtSMPL##TSPACMSPAC;SPACMSVER4.0SVER VSVER,,0 SatdScal@Scan@TUBEC10Tmpr<UserphARphCH;phDYbigphQLcphTRphTROThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 1 APrV1APrX}}1ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTSVBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA DATA iDATA &;DATA <yTDATA <yΞDATA <yHHDATA <yDCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 09EVNT 0EEVNT0QEVNT0dFTabeFVoce FWO_GATCFeat//dGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANELLAST~~U9LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;OfScP1AMVI3P1RLVFP1WDVKP2AMVQ7P2BAVVSP2RLVNPBASVV=-PBASVV;PCONVVE1PCONVVCPDMFTPDMF;PLOCVA/PLOCV>PSZE`PTYPJPXLBRGNmJRMXV1RMdNBRMdV1RMdXBRPrNJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0wRunN""JS/N%;SCAN@SMED SMLtSMPL##TSPACMSPAC;SPACMSVER4.0SVER VSVER,,0 SatdScal@Scan@TUBEC10Tmpr<UserphARphCH;phDYbigphQLcphTRphTRrIvMa3o';E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(I"]/r>:>VW|!PYW I_r{);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilVL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhi1!@MnwjuCgo&w+9m&c4QGOB;YDSc~WY|{hys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LPSZE`PTYPJPXLBRGNmJRMXV1RMdNBRMdV1RMdXBRPrNJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0wRunN""JS/N%;SCAN@SMED SMLtSMPL##TSPACMSPAC;SPACMSVER4.0SVER VSVER,,0 SatdScal@Scan@TUBEC10Tmpr<UserphARphCH;phDYbigphQLcphTRphTR