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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6be22083982c453d96c6460911fdb895Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""#####################K1313_B1_Piper_sp_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z{+CXiw7s1Pvd6|AgT 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"             '*&                 *+#        "(&          {q@tAp;AA6A_KB.bcpKB_3730_POP7_BDTv3.mobtermAACGGAACAGCAGAAAAAATGCGCAATTGAAGCTCCATCAACAATGGTAATGCTTCCTCCTTATGTTAATGTATAGGAGTTGTTGAAGGAGCAATACCCAATTTCTTGTTTTTCAGGTTTGGTATTGCTCCCCCGAATTTACTAGTGTTTTATTTACATTTAGCGGGCATCATTTTTTTTTTTAATCGTTTAGTTATATTATATTATTATTATTACTAGAATACAGGAAACCTAGTTTAAAGGGGCGGATGTGGCTAAGGGGATCAAGGAAGAGGATTGTGACCAACCATGCAAGATTTAACGGAACAGCAGAAAAAATGCGCAATTGAAGCTCCATCAACAATGGTAATGCTTCCTCCTTATGTTAATGTATAGGAGTTGTTGAAGGAGCAATACCCAATTTCTTGTTTTTCAGGTTTGGTATTGCTCCCCCGAATTTACTAGTGTTTTATTTACATTTAGCGGGCATCATTTTTTTTTTTAATCGTTTAGTTATATTATATTATTATTATTACTAGAATACAGGAAACCTAGTTTAAAGGGGCGGATGTGGCTAAGGGGATCAAGGAAGAGGATTGTGACCAACCATGCAAGATTT '07CQdnu #-;JT[jr} +7AJ[do|#2:EPZhw '0:FO[gp{-8KWcp| )3?IS_hu $-:DQ]hr| "*4@MXdlx    % 0 ; E P [ g q |   ) 5 @ J W ` k u     $ - : C P Y e o |    % / ; E V c q y  '07CQdnu #-;JT[jr} +7AJ[do|#2:EPZhw '0:FO[gp{-8KWcp| )3?IS_hu $-:DQ]hr| "*4@MXdlx    % 0 ; E P [ g q |   ) 5 @ J W ` k u     $ - : C P Y e o |    % / ; E V c q y        '""     $"(""""0;28.8.$-   '$"$""!"&00%+!1$";0 &"(&$+1&("'+" 2+( +(   ""#'  $                   '""     $"(""""0;28.8.$-   '$"$""!"&00%+!1$";0 &"(&$+1&("'+" 2+( +(   ""#'  $            2!C+)c(+P Ta2TWM-B5-P+b-#485 A! KD 6"'**;;OBG$*IH FCZ;SX] V"\ >K4cF"X;"L#Y_(FXW!3;CcD28>W'GIWQW) +7`H bX^+YM#2UQ'_ZK'-=L?YR*1Y)(T0. cRL\Y\MF?W[`O[IKZR `1%4*6<OU5>A%c8d\9E+4Q: O<CM3%! A2#W= 1%Y A+ d FdN.>^H=AA V!0GVA |\l ! e 4  R  ` $f\m)l;S#fsRcFn+xsB2Lm-1K*DFg^Zw60H\TjAIIJ0!M.%Kd S3rQ)vg#a$E5[h09ARO<aY6o6D.'Op5 )zb5s`(N=n $( U CgoF#r+eW  ( (*|HPu?OfoXXKYBUoI]UtB! 2LV 8; .)&94A'o#*)X0 $6HB 6;>7@ C.-X3/: "MWL.(-0= c.#5F8?('"r1G51$F+5$pYluxt;+BNfip'~D4a(~?p^I% p.mRgy8g:| N9iBceDZK7p5uzL%KUC {Yhi;$UnasZcpC:8Z%/?8q *'P   Zj mv  = !oqLn%|GMl6  [(/M6kl/Qj<(15:?MVb@.gh+AP6 `L@f'tY\/ia3  `WNv]01@laM$m]V K]rXE:#<v ]Kyx @kh:k8Da w /p[mTb1RU.`. )CCACACGGTTTTTTCCCCCGTGCGGGAGAGCCTCTACCTTTAGTGAAAGGATTCGTTCTTCATATGGTTGTGGGTTTCCCCTGAAGGAAGCGCTACTAGCGGCCTCCTGACCATGATCCCCAATAAAGGATTTTTTTAAATTGTACAGAAATAAATGCCACTATTATGTAGCGCGAGGGACAATCAACGAATTGCCCCCCCATAAGAATAGGGACACCATTACACTAGGTTGCGTAACTTTTTACGCTGGTGAAGCCTATTCGATTGATCGATAATCATGATTTCCATCAAACGAGACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:16:59 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtJASPtJB1PtIB1PtIBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 0 `@TDATA 0 `@DATA 0 `@3DATA 0 `@usDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISջP1AM+VP1RL+VFP1WD+VP2AM+VP2BA++P2RL+VHPBAS++PBAS++PCON++PCON++PDMFTPDMFPLOC+VښPLOC+VDPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%ճSCAN@SMED SMLtSMPLTySPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB1Tmpr<UserphARphCHphDYbigphQLcphTRphTR5This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtJASPtJB1PtIB1PtIBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 0 `@TDATA 0 `@DATA 0 `@3DATA 0 `@usDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat..GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISջP1AM+VP1RL+VFP1WD+VP2AM+VP2BA++P2RL+VHPBAS++PBAS++PCON++PCON++PDMFTPDMFPLOC+VښPLOC+VDPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%ճSCAN@SMED SMLtSMPLTySPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB1Tmpr<UserphARphCHphDYbigphQLcphTRphTRaB$T9F_"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|T921N:9h[Lr(IM=0bg(68/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH:/U?[wlpQs6G.*I9jsp)`yBp@VXY'qsC(Y5_}4]'o>s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOT1 ~>e+d;*HMNkgyKW4*L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0Fk(}=fA\d|h57N`!$OJDwPbH,pcMQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]w