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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRhTTTMMMlll  (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ac2946f4656646929c17ba5d55a7ed6dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""##################### 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'].Db}aF*mSC=@JZn#^%jE#aP}/ReQECIYpV$u:pF @n]?K~K nmNwgZQJECBCDEEFFGINU_l|KX!,$ m(So^TNLKLPZk!n.Nl&,YmSB95469;;:854469<>><9630.+*(''&&$!!!!  &.=U{aGZnFzQdPFBACEFEA;2*'.Fx7c *4`,qB+$$(,/110-(# -Ixn6&$vC#   E74{HC !!""##$#"   +pi 1ej1W    :_Ae W     #',17<@BB@>;:::::862/,)'$   0tK TRGv4   D}CdLV   +nC<2["   =+G:Q4   UKv]7R   -p@$pK   !"$%&''')+.010/,)&!0l'  f+   Q&c(    %g>E?)_$ 9tT}e)P" "'+0379<>@BDGIKMNPQQQPNLKJIIHGDA<841.,*)'%"   0MldF.   /E[mwwn]I8,+5G_uu^E.   %6EQVTK=-   +;HQSNB3$   #?aaC24GfeF/!!&**'!%3DWhsxuk\L>649EUerywn^K8( -?SfrwsgWF7,&$'+/10,'$%.>Vqt\H9/*'%$"! !!   S />>>8'>>"%>'/8>6//>>/>>>>1>>>>;;;>11;>>;>;>;>>;+;>>>>>>>>>>>>>;>>;>>>>>>;;>>;>+;>>;;>>>>;>>>>>>>>>>>>;>>>>;>>;>111>>>>>888."&$) 8>>>.+>88>>88>8>>..>8)$$$ .&4>03+(4".+/.+44&$..$.)(".. *4"%'4646:*"//4."/))44!*/   +  1 ::)4" :>/>>>8'>>"%>'/8>6//>>/>>>>1>>>>;;;>11;>>;>;>;>>;+;>>>>>>>>>>>>>;>>;>>>>>>;;>>;>+;>>;;>>>>;>>>>>>>>>>>>;>>>>;>>;>111>>>>>888."&$) 8>>>.+>88>>88>8>>..>8)$$$ .&4>03+(4".+/.+44&$..$.)(".. *4"%'4646:*"//4."/))44!*/  2VBTF 3E_-5E&b"/V2J1XN^95b(UMZAZ)(H:VVON5&>] X\[cYCWNR58/+HY,=#R\&=M;;&2SJ< R.J 0#&NF 1X\=FUP&P]8DK;bN.J]%@>7\-]E>  5/a61R@IL S!#N@GaF%=Z0X2;EI(b@2J^ \B\;&)1=T_#AT9$K_8T[!GZ'651( NR`A\C$.0SK4O",0RMLFU ]? -N3&;^FU)$=E;$ `,.Uh+rvT"m :# 14Q4Tc+:EMD^Z6saU#t;kr.Z|#Uw5{N+'~}4BG C<\&`\%G0vWnMEJCsp;$0!~A}q U5V%C\kI";5(:\R]bvzXt?oB *n:.  q()  BP~h(fo-]J"pga 3 X7}8-~42?|pi|^XNhYN:58xCK<C.'>!-?*D<>2-".34%  %      #        !"  $! $ )  )    &bdpp+'-pgppJs-d_B,p!/=pzL s8dd+dId6!Hd%-|8`rWfXB<-u<m:M g#\@+"*E3'^0 vY, T6!*ND@" L3 * "(,($#1PF1! 8  .1<? i,nLXIOKG jMF!w@0 %8fj*,bzk`,/SqD1QOEDp*!vg~ayZpY>o9A^O`Jku=mL{>ZLaZZ+CACCTCTAACAATATGCGGTATAGTACTACATATACGTACATATTATCCCTATATAATCTTGTAGGGAGAGTGCCCTTTAATGGCATAACCATTCCTCTCCTATGACGAGGATGAGCCCACCGGGACCATTACCCACCCTCAGATACGAATGGGGGGGGGGGGAAGGTGGTATTACAAGGTGCAATAACCCCCCCCCCTAATAGGAGTGGAATAATATAGAATATGACCGCATGTGTCGTCGTGTAGAACGACGGTGTATAGTGAGTCTTCAACGAAGGCGAGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:18:03 -04:00AEPt?%AEPt?%APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtWASPtWB1PtVB1PtVBCTSiBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?VTDATA ?VDATA ?VSfDATA ?VҼDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AM<]yP1RL<[=P1WD<_P2AM<d-P2BAfiP2RL<aPBASSkPBASRMPCONZPCONYPDMFTPDMFR1PLOC<VPLOC<TPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%RSCAN@SMED SMLtSMPL!!TySPACMSPACR*SPACMSVER4.0SVER iSVER,,/Scal@Scan@TUBEA9Tmpr<UserphARphCHRHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt?%AEPt?%APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtWASPtWB1PtVB1PtVBCTSiBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?VTDATA ?VDATA ?VSfDATA ?VҼDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabwkFVocwZFWO_GATCFeat//w+GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AM<]yP1RL<[=P1WD<_P2AM<d-P2BAfiP2RL<aPBASSkPBASRMPCONZPCONYPDMFTPDMFR1PLOC<VPLOC<TPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%RSCAN@SMED SMLtSMPL!!TySPACMSPACR*SPACMSVER4.0SVER iSVER,,/Scal@Scan@TUBEA9Tmpr<UserphARphCHRHphDYbigphQLcphTRphTR6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGxNM(p:F2O}MK>Q;(AZ>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"H2KQg`H#t;MjIALsRkK_n9Nfcd]R039OQJM~V[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %> Ube1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&4&SG1T1M};<r~ .:S#C&Gb&hf73)bT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^iAEPt?%AEPt?%APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtWASPtWAUDT~B1PtVB1PtVBCTSiBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?VTDATA ?VDATA ?VSfDATA ?VҼDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabwkFVocwZFWO_GATCFeat//w+GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AM<]yP1RL<[=P1WD<_P2AM<d-P2BAfiP2RL<aPBASSkPBASRMPCONZPCONYPDMFTPDMFR1PLOC<VPLOC<TPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%RSCAN@SMED SMLtSMPL!!TySPACMSPACR*SPACMSVER4.0SVER iSVER,,/Scal@Scan@TUBEA9Tmpr<UserphARphCHRHphDYbigphQLcphTRphTR