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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d0372e1b9ae348019176053625c1ac6bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""#####################K1313_A8_Sida_sp_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z&&''('&%#""#%'*++***+-/12333468;<=<;:9:<>@A@=:88:>BEDB=964431.*&" 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(3AQanwytj]NA7/*(&$" : 0|@A!FAPU2AdKB.bcpKB_3730_POP7_BDTv3.mobtermACCTCTCTGGACTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTCTGGTTTTAGTGTATACGAGTTTTTGAAAATAAAGGAGCAATAACCAATTTCTTGTTCTATCAAGAGAATTAGTATTGCTCCTTTATTTATATTTAGGAGTTTTTCTTTTCTTTACATAAGTTTTTTTTTTCTTTATTTTTACTTTTTTTTATCCTATCCAATCTTTTGTAAAGTTTTTCTTTTTATTTTAGGGTAAAAATAAAGAAAAAAAAAAGATATAGTAAAAGATATAAATTGTAATTTTCTATATATTTTATCTCAGAAATAAGATAAAAAAGAATGATGAATGATATAAATTGAAATTTTTGAATTTTTTGTTTGAACTATTTAAACAATAAAAAAAATACTATTATAGTTATAGTAGAGGGGCGGATGTAGCCAAGTGTTATCAAGGCAGTGGATTGTGAATCCACCATGCGCAAAAGAACCTCTCTGGACTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTCTGGTTTTAGTGTATACGAGTTTTTGAAAATAAAGGAGCAATAACCAATTTCTTGTTCTATCAAGAGAATTAGTATTGCTCCTTTATTTATATTTAGGAGTTTTTCTTTTCTTTACATAAGTTTTTTTTTTCTTTATTTTTACTTTTTTTTATCCTATCCAATCTTTTGTAAAGTTTTTCTTTTTATTTTAGGGTAAAAATAAAGAAAAAAAAAAGATATAGTAAAAGATATAAATTGTAATTTTCTATATATTTTATCTCAGAAATAAGATAAAAAAGAATGATGAATGATATAAATTGAAATTTTTGAATTTTTTGTTTGAACTATTTAAACAATAAAAAAAATACTATTATAGTTATAGTAGAGGGGCGGATGTAGCCAAGTGTTATCAAGGCAGTGGATTGTGAATCCACCATGCGCAAAAGA+:TYlu|.BQ\gt~  -7ER[gu )4BN[ht #.8CNYeq| #.9EOZfs #.;HT_jv&2>IVbo{*6CO[gs~  % 1 = J V b l w   , 9 D O Z e r ~   & 2 = I V c n y    * 5 A L X d q {  , 8 D P \ i u '3@KVco{ ,8DP]hs(3@MYeq| &1=IVbmx,7COZgq}&3?KWdp}'3@LV_jq}'3?KXdnz$/=+:TYlu|.BQ\gt~  -7ER[gu )4BN[ht #.8CNYeq| #.9EOZfs #.;HT_jv&2>IVbo{*6CO[gs~  % 1 = J V b l w   , 9 D O Z e r ~   & 2 = I V c n y    * 5 A L X d q {  , 8 D P \ i u '3@KVco{ ,8DP]hs(3@MYeq| &1=IVbmx,7COZgq}&3?KWdp}'3@LV_jq}'3?KXdnz$/= " ",'.-#6:'4) 64$>>6/8/>+>>//>>-,1;;3>+>;>>>>>>>%!>;;60>>>>>>;>>>>>>>;;>>>6>>>>>>>>>>;>>>>;>66>;>>>;>;;>>>>>;>>>6>>>>>;;>>>>>>>>>>>>>>>>>;>>;>>>>>>>>>>>>>>>>>;>>>>>;>;.0+""""6;>>>>>>>6.>>>>>>>>;;;;>>>66>;8666>>>6>>;;>>>>;;>>>>>>>>>>>>>668>6;;>>>;>>;;>8..;>>>>>>1;;;;>1;;3>>>>>>>>>>;>.8;->>>>>>8;;>>;>>88>6/>>/>88/>83>8..8>8832238888>>>>>>>>>>888888/8>88888>8/8/8/+8444-044"/4(>2444>>>>>>>"/4643:64-*6444614/  44#"66:4*-::"4,4"()   " ",'.-#6:'4) 64$>>6/8/>+>>//>>-,1;;3>+>;>>>>>>>%!>;;60>>>>>>;>>>>>>>;;>>>6>>>>>>>>>>;>>>>;>66>;>>>;>;;>>>>>;>>>6>>>>>;;>>>>>>>>>>>>>>>>>;>>;>>>>>>>>>>>>>>>>>;>>>>>;>;.0+""""6;>>>>>>>6.>>>>>>>>;;;;>>>66>;8666>>>6>>;;>>>>;;>>>>>>>>>>>>>668>6;;>>>;>>;;>8..;>>>>>>1;;;;>1;;3>>>>>>>>>>;>.8;->>>>>>8;;>>;>>88>6/>>/>88/>83>8..8>8832238888>>>>>>>>>>888888/8>88888>8/8/8/+8444-044"/4(>2444>>>>>>>"/4643:64-*6444614/  44#"66:4*-::"4,4"()  ,b_$)I9 %E !CF28P? P M@VG!?BF,?\' P,\G;b>CQc. '!5TL.-KF 2S`:. G7>.FUU/+!:C<ZK@'VJU``[ #(.5^G?=HXT.T4C13U0V&"/BI'\FJFNE"#2_2\#4NRY-!@094GP '5=BT&=[9S[)9-[5N_  ,=5J%'>KIT E2Z2Q6;a/03K9 6[ ,L&3 _>+68U2(/6U!? FX+b`O XXS`GDJ *D?";; C3@&Q0S&WP7S+<0B$3DYCWG#%()Y>JNLYQ/4 U+%[Z^(:2.;MD2'RH5&T 7MB3b`*>U<0Gd*/.&><>()( (&C*_UNAYL1'bAK*A5RK8S PH@ 7ED^>4;C=RD. SW<'FMIXJ('*R::C2"M VBqi' UY7FFA/~@C)%^eJ;x$?z@1_K&S6U+EcC .#Vw-njC9(A}XE /V%,^>'/z,% +Su`Qt{~&I!/`(Gq[2Q{ h Cf}Oj#*L3Ch*FQpcwo%)RT773<a_`:e2> dGx# x*GZbW(0c`;;G|!r}^~YHc-x.mAzTY zPU!TC]EfT& mfr3e5 Fa 09ICSLy]K46@}shc \eKoWvkQx@@<;??A51;G/4-<248-)  '. #          xxxxxxx xx xxxxxxxxx         &  9/$4#L##6"4%),0-%:)#K?7#  36R  S;0:  &(1DA%8--[- -!B`ppmfeIniyp}4Iwd@0V2`d?F_z\|?s2T/-WdLiq_@TM8+  uJVd,OP l!87R%-!3]K/rh'p(2xpX6[ d@pW(or|+9*z9SkdNid'lpXA.-8dfp/-R5?ksqfppx%izin9B\STO2D&osM '8%)C=y\E88Y]L " Hel%:> -. +I! E %%LR\,& &      *#    )IG/ :%- (//(-OC!8m"B$C3-" Q%)#3A (/#-o F/K@!*"9| :,: 'SC/HA'+K4"5);'7by;Z;"?HE$KMNKK;!mE_\H:(Jz/3:'*abE$;sU794RJ"T=+MUtO<qyF<gB3$(#\QAQ4~bM]fKz4>3KQ',KYOK;P3@.Z7NW$?Di|/T~"Heb8Q%aeH`"(dHg-_Hx L `%1CAACTCTAAACAATATGCGATAGAGCGCTACATATACGTAAATAGGACACTCCTGAAATTGTGTATACGTGAACCCCGGTAGGGAAGCGCTACGGTCTGCCTCGTGATATAACCAGCCCAAGAGGGGCGCGGCAAGAAATATAAAGAAGAGAACCGCCAATCAAGGGGGGCAGAGCCAACCTCAAGCCCAACTCCCCCCCAGAAAATAAACGATAAACTGTGGAGAAAATGGGAATGGGGGCCTAGGGGTGTGGTTGGGGGGCGGATATCTTATGGGAGGGAGGGAGAAGCCCCCTATATATAACAGCGCGGCCTTACCTTCTCTCTTTGGGTGGCGGGAGATGGGGGGTGGGCCCCGTTTAACCAAAACGCGCAATCCCCCCACCCCGGGTCTTATTCCCATGTTGATAGAGCTTCCCTACCCACCCCGTCTTGTGGTGTTTTTTTAATCTAGACTTTCTTGGACCAAAAGGGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:17:49 -04:00AEPt=hAEPt=hAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtZASPtZB1PtYB1PtYBCTSqUBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA >}TDATA >}ҐDATA >}PnDATA >}LDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISL2P1AM_P1RL[MP1WDbP2AMj5P2BAmP2RLf{PBASNBPBASLePCONYpPCONWPDMFTPDMFLIPLOCSPLOCPPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%L*SCAN@SMED SMLtSMPLTySPACA_ SPACLBSPACA_ SVER4.0SVER qJSVER,,/Scal@Scan@TUBEA8Tmpr<UserphARphCHL`phDYbigphQLcphTRphTRӁThis is the confidence range *ABI_Limits..FVocAEPt=hAEPt=hAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtZASPtZB1PtYB1PtYBCTSqUBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA >}TDATA >}ҐDATA >}PnDATA >}LDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//pGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISL2P1AM_P1RL[MP1WDbP2AMj5P2BAmP2RLf{PBASNBPBASLePCONYpPCONWPDMFTPDMFLIPLOCSPLOCPPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%L*SCAN@SMED SMLtSMPLTySPACA_ SPACLBSPACA_ SVER4.0SVER qJSVER,,/Scal@Scan@TUBEA8Tmpr<UserphARphCHL`phDYbigphQLcphTRphTRTFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh("X19t$D&a_+)Yo].5s|wL}TKS]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0SbXW,VE+,[Ol% eIe]xP!t p7qf j O5TwARM}TDATA >}ҐDATA >}PnDATA >}LDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//pGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISL2P1AM_P1RL[MP1WDbP2AMj5P2BAmP2RLf{PBASNBPBASLePCONYpPCONWPDMFTPDMFLIPLOCSPLOCPPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%L*SCAN@SMED SMLtSMPLTySPACA_ SPACLBSPACA_ SVER4.0SVER qJSVER,,/Scal@Scan@TUBEA8Tmpr<UserphARphCHL`phDYbigphQLcphTRphTR