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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bf87e73ea2ed45459c48500119f4dd7bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""#####################&K1313_A4_Asclepias_curassavica_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z 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'  + %  (. /!3$3!4'BD+!*9 !( !-(%%+/.7+7AA/2#%C *  &G6K3g43'5SBB)6C7BBBBBBBIHb'8<f"BJH9>Q6Nh*4****Ye*YQUUU`{GeL]cGU6GM[MvO]a_ZoPIUa[B**VY4uLZ^^t^U^UUUBBBBB ppp8p{pp` ;L~yQp;6k8d-. dtm:"6 nY&tD&~q?j|4nTS,c"&> +)/QBd3*2AQd91wz!kApD1"5U<."  '9,$\45!! /9    K4@3>!Lc)"5.  L1,UWXB'/A7&0/0! )#31a1AO@R#&$/ %4U%J<,1>G=<"*&':LGC&]5$f7-9-&3;)O(.=-RC(ZJ1WQ;ZCQpeB91$5Yt_;$>;K-=4,1V?@N'AKB532JZ }CCmq9K=9kjFT0B 1<%Tq>j=)6f'bP(X~<epm;+ZVn.k|XATCTCTAACAATATGCGATATAGCGCTACATATAACGGACGATGATAGCCACCCTCCGTGTGTATAAGTGCCCCCTTGGTAGGGAAGAGCCTGTATCCTCCTCAATATATCACGACCCCCTTAGGGCGCTTCAAGGGCCTCCATATAAATGATACAGGAACGTTTAAACGTCGATATTTGTTACCTGGTTGGTCCCCCTCGGGTTGGTAATCTTTATTGTGGTCTTTTGTATTCGCGAGGAGGGTGGGAATTCTGCGAGCGGAGACGCCCCTCGGGTTACATCTCTTTTTATTCCGAGGGAATCGGGGATAGGGCGAAGGGACCACTGGACAGGGATAATTGGGCGCGATAGCACCCCCTTGGGGAATCCAGGGGATGGATTTAGCGCCCCTATGTTATTTAAATCCCCCCGGGCCCCAACTGGGGATTTATTGTTATGAAATTATTCCCGCTTATGTTGACCCGTTTATCGAAGGAGTTTAATGTGGATTCTACATGCGCACCCCCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:17:17 -04:00AEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtCASPtCB1PtBB1PtBBCTSyBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?2TDATA ?2DATA ?2S$DATA ?2VDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMeP1RLaP1WDiP2AMqP2BAuP2RLmPBASSPBASQPCON_PCON]PDMFTPDMFQPLOCYPLOCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%QSCAN@SMED SMLtSMPL''TySPACA\ SPACQSPACA\ SVER4.0SVER ySVER,,/Scal@Scan@TUBEA4Tmpr<UserphARphCHQphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtCASPtCB1PtBB1PtBBCTSyBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?2TDATA ?2DATA ?2S$DATA ?2VDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabnFVoc]FWO_GATCFeat//.GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMeP1RLaP1WDiP2AMqP2BAuP2RLmPBASSPBASQPCON_PCON]PDMFTPDMFQPLOCYPLOCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%QSCAN@SMED SMLtSMPL''TySPACA\ SPACQSPACA\ SVER4.0SVER ySVER,,/Scal@Scan@TUBEA4Tmpr<UserphARphCHQphDYbigphQLcphTRphTRP'd}|(Hfz@jXa*`#|nh-u?[-0#Osp_0_9v8ZIYW<|eN$&i[D:0M,m(o~%Md02R P,hzp!tY`-'Icf]h_m7^d`7Y{v]qoUYoOyl}$r&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hDM=VrTH&zqru+!hs|O1i~?z;{W8<)mbo)6s6IxO\JpSbwEiv5&o58J.:)'C#x5ots/H1C:2#%=c/a}ayHbc\S.Tqt6IEUYC~n{[._LbR/"E.Om,NeLVWY+a:WkeC.%oh^5Q1V?|&@8je]i=mcR=T3W-^ rY/gc{Xm|3HF)SE8n\qoAq&B6yI%E|"Ow_@w-F96P3:Wo6GS#C&Gb&hf738bT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m*0_fs$X=<;#(eaK|`!t/[z~+7K>0Nt'?'|URmquV4wGl%R7R>(hZ$~I!|spN$t.Dd^4G^({C!RJ<6Q^Zew.k.d}uNf1&vM\#YTEl?&az~#FYMu>K_R_K4%,#`C?82SY|~wA1GJWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG