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GGG<@@ZZZooo  !!!(((0//666===CCCIIIOOOUUU[[[aa`ggglllqquvv{{{~~~~}}}~}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}|}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}}}}}}}}}}}~}}}}~}}}}}}~~~~}}}}}}~~~~~}~}~}~~~}~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 051424cdc3d24b3e8c08fad30a11a68cSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-04_00-51_01473130Seq1285512855_Steier_K1322_psbAjmadera96-Well K1322_F1_psbAKB_3730_POP7_BDTv3.mobSeqPop7z,DV_]ULGFGGC=8:BO[^VF6+,7GT[ZWV[eqxvl\LB>@DIMRW_hmkcUG@ALYdgaXRUas "/=IOMF=659=?;1" #)#sV>0.5@HG;) .>FB6( !+9EJG?99AO]fe[MB@FQYXL8$!*/.*&&-8GVdpy  dG5* |utx|$Q}|zfI( (1."&@SXN9$$4?B=624:?>5$%9PmDyRk(3L^ebXK@::BQiP=9p{N W}L*3 <ia +MG;X&#OzZ&<o%fyf]YSH9(/PB]c$uo0@@ *a]b7    '(%!"%'&)6U@?MiSf<   2Mev|o\E-!$%%(,02-$#<Ys/33Z?|b{mcec>,.B`~gQ<)   &2=CD@80)')/9ETg| k95W((2;?<3&   '-+$ 3ENK;%"O"UnkR+^A+  $,252,#   #/:DMU]cir`}3oW>)$*+&    "" "!'=Xq~woinFf!tM.#4BIE8('08AKU`imldUA+  &4<<5) $'# &))(&%',4=FLMIB=BWAwYs7 +486-  "    (0:Kc}55P:I?o$Cj'6]gON)  4FRRG4  !,6=?;1#        $1881%#5J[efgpD{o6Y9!  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T{K@=qAA;7A'RKB.bcpKB_3730_POP7_BDTv3.mobtermGGAGAATGGGGGGGGACCGATCTTGCTAGCTGCTGTAGAGTTCTGTCTACAAATGGATAAAACTTCAGTATTAGTCTATATGATTTATTGAAAATAAAGATAAAGGGGCAATAATAAACTTCGTTTGGTATTACTCCATTATCTTTAAAAAATAGAATAAGAAAATAATTAAAAAAATTTTCTACCTAACCAAAGCAGAATACCTGGAGGGAACTTTAAAACTGTTAAAAGTATCGAGGAAAAAAAAGAAGGAGCATACCAACCGAAATTTATTATTGCCCCCTTTTCTTTTTTTACTACCTCAATTGACTAAGGAGAATGGGGGGGGACCGATCTTGCTAGCTGCTGTAGAGTTCTGTCTACAAATGGATAAAACTTCAGTATTAGTCTATATGATTTATTGAAAATAAAGATAAAGGGGCAATAATAAACTTCGTTTGGTATTACTCCATTATCTTTAAAAAATAGAATAAGAAAATAATTAAAAAAATTTTCTACCTAACCAAAGCAGAATACCTGGAGGGAACTTTAAAACTGTTAAAAGTATCGAGGAAAAAAAAGAAGGAGCATACCAACCGAAATTTATTATTGCCCCCTTTTCTTTTTTTACTACCTCAATTGACTAA*7CPZmw+;M_gx )2?JXdnz)6CMYfq{ "/8EP^ku ".9EP\fs(2<JVboy $-<DP]jv $19GR]hr   % 0 A N a q x    ( 4 E O [ g p ~   % . 8 B K W ` j x    ( 2 ? S _ q {   ( / < K R ` k t  )8JTcmz*7CPZmw+;M_gx )2?JXdnz)6CMYfq{ "/8EP^ku ".9EP\fs(2<JVboy $-<DP]jv $19GR]hr   % 0 A N a q x    ( 4 E O [ g p ~   % . 8 B K W ` j x    ( 2 ? S _ q {   ( / < K R ` k t  )8JTcmz   &  $'+ +0"3+2;1.&)8'+11+(   &""""                                                  &  $'+ +0"3+2;1.&)8'+11+(   &""""                                               8%C 73E+US'_=ZH^6;_- P!G TAMWH?#?BPO?$T)L<,@Z ?:G+F"'CZ<TIMS SPLB, PX@4H@(RQ_U N A$"0"U /P\#2U6X ' ^'0<#NLY  d P S\UU5([0bTL9aU%OU49H)C5R;-# O4RRR?>V5IEC[A=1K6(NDQ'cL5@>-M1 "#?5$:!56c)$C!V'.5`3N?aS]OCP/SG>*Z0b$(#.M%J5EV[.Z$V,H& 2!rc @t7szNm 7vf2lEc,U c&u Y9`!2Wg=]}5fQD=p,LR qEUR(: 1H\:9VaD9`zcqOHmqN6#EgS8Pme5(WB[rJ?z cUYC!|1@ 4No#f%ls]TA.1.ac?yb tKi:fdP)0+-PB-`[FY|BEM]vt:_\]9Z_dP9W>MLKX[L#2>D5=C3$^"Xyg[|V!f|7eG9\()* +?C{ 2v65Dp:p6pFrppC+5;(8L{>pnt_1AMnux?O$Isf'3jw!d+a mGt, 6 %U'Apd=l`R+>VM9PN#EoJk^'kq%I_lRa%P1 DB<7%xLE]8-;.+8Dz{AiBt] t qd.L`fqL>e 9@tV,  $"_"5+eK:=No{6Ir+X$c qgi77 z*;]!9 ]x.5vP[ lU~TEh6X' ;X~{RHE[ x3 7LBrd] & kh9 io]NTK2!PSR4V*wrAAGATTGTTTTAAAACAAATATCAAACGAGGCGGTGGACAGCTCTCAACAGTTGTAGATTCCTCCAGTATAATTCTATATGAGCCCTCGAGTGTATGGATATTGTACCACCCCTCCTCTCCGTGGCCCCTAAGACTATAACCTCAATTTCCTATTTTGTTTGTCGACCGGTTTTTGCAGGATCTAAATTAGTTCATTCTTCCTTATAGATACCTCAACGTCTCTCACCCCTACCTAGTTTCTTTGGCATGACGCACCTTACGAAACTGCCCTCCCCCTGATTTCAAAAAAAACAACACGATATTCGAACTCGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-04 02:21:42 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTSBufT'NQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,_DATA #GsDATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM9rP1RL9r P1WD9rP2AM9rP2BA99RP2RL9rnPBAS99PBAS99PPCON99 PCON99 PDMFPDMF4PLOC9r4PLOC9rPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 3 RUNT RUNT  RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPAC-SPACMSVER4.0SVER  SVER,,Scal@Scan3TUBEF1Tmpr<UserphARphCHKphDYbigphQLcphTRphTR+nThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTSBufT'NQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GDATA #G,_DATA #GsDATA #GDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab*nFVoc*]FWO_GATCFeat..*/GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM9rP1RL9r P1WD9rP2AM9rP2BA99RP2RL9rnPBAS99PBAS99PPCON99 PCON99 PDMFPDMF4PLOC9r4PLOC9rPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 3 RUNT RUNT  RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPAC-SPACMSVER4.0SVER  SVER,,Scal@Scan3TUBEF1Tmpr<UserphARphCHKphDYbigphQLcphTRphTR-&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv:Q(#Jar41 ~i|]-@[3>>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`]vWs*D_rj|YnG|urG&/o{o;Mj2Q&?R2gcV]j4 VI0U