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N M W 8 F : @ a G Y B H 4 F > U F 9 I 8 J T Z F < A V Q 2 _ : B 2 h Z Y K D 0 S T Z C F / A Z A N H I D G L X O K W 7 O J ? E ) K ; @ R S ; L O N F N R H 2 D D G Q . P @ ] Q : L V ? 4 H E J E < 7 \ X 3 L Q T V G R M 8 D R @ O P I O E J g @ O U M U N K U C @ N T ? : K N H N B S _ J V J D F = a S Z ; J \ \ C W D C ` T H L ^ @ 2 6 C M W D @ @ T \ B G ? @ ; J L D ; R r R H N i : B = F 5 ? 9 A 7 3 . H 5 O P 1 @ 3 ? P Q S f : F J 8 F [ B A U _ V , L V O w A : @ M A N A J O [ J ` ` G D ] D ] A ; H ; 3 M N L L 0 D T ` X P E A < B ; [ L L E X < S R > @ Y J 3 G C N W E = E > N V > @ : X L S 7 B ^ ] : J E 9 0 3 C G J H Y P < C M 3 7 4 I O I N M G E K R M & H A G ] L A F U c Q [ ] G @ b C ] V D I X > J V = M D O P V S D H s D T T H c O E ? E 2 L O E 5 > Q < ; N X 0 @ [ 2 6 L ; L < 9 K F \ M M 7 ? : U N < ] I R H a Z < T = C B E _ N I X c _ O = h Y L E < L > 4 F f C P H C e < = Y I R ? > ; ; B [ H b L Z 7 2 J . N @ 9 F P I E < N T M V 8 0 M < D < I 2 ` ^ s = U F @ ? 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B D K H ; E D 3 < h K H + D O & D K I J H 2 @ 1 L J ? E P H @ E : : = J G K R / O 8 7 < T 8 K . > B 4 ; B - H L \ J B H O : O E ^ R 7 A C O O @ P H L ; N H I F d N * O T > 9 : R I @ A Q L F T Q J M W K Z M 8 G ? ` O ; W I ; 2 V E _ f S ` G < J W \ F C 1 Y [ ] L > L M C B C L C \ ^ ] J D L I M < 8 G R c @ = X D H a 8 M S W G 0 \ 0 @ F I K 2 J E M 9 K N > M R L > M F ; \ 7 P L R : g O D F G H C 9 A C D 3 4 F Y B S A = \ ] R H E . R H 6 / 3 Y ] 9 6 I 7 6 1 " , ; H c C N ? K T 9 Q 2 E = H I N S 2 H @ B A G 0 7 Q @ 7 3 U ? b P V F M 8 C D 7 6 V R L L V 5 J ? Q U K 9 A L V e B Q L I S J D G ; F L R T e R 6 J O O H G U J P H D G R I C H ' : , L K @ H O > F H ` b O 4 C 7 S L A C S H V N X J @ H 1 H K K ` V J 9 \ 7 V ^ I S Q > D Q @ 5 G F A f ^ = P F [ \ \ < A m G [ B E D F 1 ? ' N ; G ] D , + < C N H 4 K K 9 c 5 1 S L J g A A ; h F 9 F > = 0 F R V g [ g I P G 8 9 : L [ R _ L M b = O ' F @ H Y F L L / ; J E S ? 8 " < @ G ) > ) @ < : H U D A < B > L R G Q H 2 > 2 > J 0 F < ` b A R 6 H B 6 L 7 7 ? 7 O V K ; K + E @ W . C Q ? 2 D Q J = B * / G L G O B H K G B = 1 > < = S X @ < _ P L @ M > : B ; N \ T 8 @ % i 7 ? F < L F M ( B 8 8 D D L 7 3 2 @ 8 8 ; 9 G Q R M A C > < @ : G J B I J F G G L @ ? : Y @ E \ D ; J * > ; ? > > @ G M U I D 2 R = F O / U 3 A T E O I ? / = @ 6 ; Y 0 J S [ Z a \ Y G . 6 E 8 6 H M 4 H 9 = 8 W 8 . @ V k @ F K K E G 9 I + Z 1 9 O 7 S @ A A J R = J < 8 > > : [ 2 P a \ N U W S T C Y L L ^ 6 - A = 6 D + E C 7 E N L J D 7 U b R X 1 O 8 - 5 . = L ' > B H 5 = S @ C ' h 8 0 2 H B J K N D A < F 8 D : G U S H L P C L O I E _ F J W ? 8 8 A D T T > W V Q U B ; V E F 6 0 J E 4 G I A B 4 6 E 5 M K > ( 8 D 3 6 M a ? _ I = O ^ = O Y V L U C + ; L F < ? - + c . F Z A ] A 5 Q F J o G N G > K H @ ? E ? V V C 2 6 X G ? H G Q 9 < < 7 G B R K : < - = 5 F N ? S < O ; / > K B E 1 E T ) M D N X R D Y C 3 ^ < 6 : L T V W ; : 7 ` @ O Y @ E E Z N ? ? 5 1 J < 8 ) 5 F a 9 & W : 1 ; E 6 D < 2 O H P g B ` @ R M Q < E ` 1 I , D 4 1 L N M J N c H H \ b @ S K N = 8 J C 8 0 J F ' J E @ E L U > T B T 6 8 H # J P ] F Z V ; T 3 - & O C P G B Y T C N M = F V F G M Y N ? # 8 C a 8 @ E J R = ? ; 6 N A F ; F C 2 = 5 : c > X D E = H X Z D Y Z O H R S P 5 O S U @ 9 H G X N = 6 @ 6 R g B M 8 = I U O N C D B C Y ^ _ H S 1 H E A = 5 @ ; C G I S S ] H ` S L ; . ) F 6 D 6 ? F ) @ C 4 Q T P W @ 5 W ` A 4 P : - B R ^ 9 A F C B C J M K B R E H 8 @ D L G @ G G A C P N ? 4 K A ; T = D G I I 3 C 3 D O E T > D ' 0 S ? = H T 4 G @ N N N = T 4 ) X C P D F P M M H F 9 B Q 9 7 7 4 F B I , 5 J N D F L N B 3 Q W F C > B H < A C Z H < Q U F B A W L d X @ A T < K 8 6 O ) J R N V A L R I K b O M A J H V ` V T W U > T : M ; 9 L : C V : [ < % H C ? D A Q 7 I H 9 4 ] _ > Z F D P G @ O I P ] c F N < F Q ? ( 7 ? @ > W \ G R K = E 6 G @ ; A > @ ? ! A 0 G = C E 8 F 8 ^ 9 G L Y E = b F 2 > ? H B e C \ S [ W O < 3 @ 1 W C R I ^ C J D B F M V J < D A W J 8 M E ; @ A E Y ? O F B D A M W E G ? F 9 3 1 J R V 9 I B 7 7 U > @ O H 6 F S S G Y F P T : ; G 0 ^ = J U B J G W W > S N G + & 9 : E J T G 3 4 C B 3 J A L 0 * ( 9 = E 6 > > F + > Z P D @ M P K D B K - 4 Q F < G B C 8 * % ` < V D K U D O G 9 5 = ( N J Y 7 E C = L J + . 5 F D ` ; ? S 9 7 < ? 8 # G - % B * [ 9 9 T Q K Q > : R N Q J P M L A P . H 7 @ 9 < L R d E D N D : N T B 6 H C Q L K [ R L 8 H K $ 6 B M C > E 7 4 Q 1 H ? 6 : 8 0 7 = % ( O M & E M ] S M ? 3 7 A : J C ? : - ; > 2 > 6 H @ ; . O Y > ? A ] D @ M W G = E E @ N B 9 L M ? = K ? 3 S I ( S E 5 ; D @ > C F > P & @ 3 @ O + , L = ; V U G L ? 3 7 I @ j H H F N : W ) L = R G 9 H @ G < V L > W T E , L J ? E 7 J 7 G H H ; E ` N 9 B F F < D F F , 1 5 5 6 ; " : ^ C ; ; E M C G ( > E C > / @ K 5 G 2 O B S D K X ? 8 H E H 8 G N : 4 2 E D X 4 K E ' - 7 S C ( 1 P I F T N [ 8 H : 7 I M L N Z < ; I 1 1 @ 5 R W R K F A @ : N ; 0 B O G > G B N J A E T I D M & ^ 2 H ' S 2 < D ` G G I H I J K V % > D M 0 C C ) R N C Y G E + ? & 4 B ? C 2 < 2 : W U C B M ; H 8 D 2 : ; 2 F 8 P H / G : 4 7 @ @ Z 8 H B 8 5 [ 0 * : J 6 V 1 Y S U H \ J S H : * 1 + I N I < H 3 / I ; , D : B b C ; : # C R P P B G D @ B K 9 j 8 I T G 6 I 9 K O P . : 9 Q @ D X . O X ] 9 K < 9 D M & L + E . R 4 7 > E L . E B ; E ) 5 - ( 6 L J 2 H 4 8 E G : R ) A H E ? $ > = H I ? U M N 2 I B 3 I % 9 L : N ; 6 I E F % @ 4 < M F & : H M : J 6 . B @ J 5 W C E 9 ( E ; K 3 ? 0 E J E O @ / K C - N K < @ Q ] W A > > I F @ C ; @ 0 2 = / P S C 2 < + 3 4 A 7 , 4 Q U 9 @ T - ) 4 S G S ? K 0 1 J = H 9 J P 0 E D A ; Q 2 E B H 7 8 ; > & O L B M & Q 6 > : F ? ' H M O T E ` F 4 C . S N 2 , D M J 7 F 1 T ; ; P H + > C B > Y M , D _ ' 9 4 8 C S - Z E 4 T 2 ? B R I 9 < 5 # = B / * [ [ H L P F B H 2 Q < E G * H 3 B 8 _ C E F # 5 > ? B > < E % H J h Y P ? 2 ( H E D S 2 " = > 7 T ) ( 0 D P < - 5 4 K M & - L K @ : Z N g U K U C C ? C = 8 , ) > E 8 * ? < 7 8 3 C P > 6 5 ? J > N O D ? ] L > = Z B ? L 4 , 3 5 " 2 > 6 Q ; 8 / H 7 = - 1 : 5 9 < O c 4 V P C H J < J ; M K G . 2 A C G K @ ( J & A F ? Q 5 A 3 6 8 ; ! ; # - ' B F 1 C K > B 8 ; > : 4 A 3 1 N H ? M 6 = 4 1 . L 4 E 7 ; > 8 H 7 7 5 ) 3 M 8 G > ( 4 T 6 S D 8 N P I 7 < < 9 N S K > ? B T 5 1 8 ) 0 ; ? + ? L 9 ? E 1 I @ 0 P ; A , 6 9 < [ K D 4 K P C ) / 1 P > 1 F A \ Q L ; Z ? < @ O B , : U @ < D % 2 ; 8 P D E Q b I D B 4 > U , N @ M N 2 N / 9 6 6 * N O _ = V ? D G B I D 7 > ^ 8 < > 7 @ , + F J < T M @ < [ M < @ 9 > Q 3 : 2 ; , = J C ? N @ j A 6 O ? A I K 1 ? 9 $ 9 G " 8 / = % S H 8 D 9 M < E D A H F J 0 E ^ H 5 O _ I 6 * G J J 7 * E 9 J - F D + & S e V 6 C H L E E ; L I C ; ? 3 = - - 8 G R ] f J 3 * L ? G P / E 4 S D 5 @ F O B E : J K > O 3 7 @ K > 6 * 4 < / A S I R V V A ; I 4 - < ( 9 G H @ B D > 3 E H J A J P 7 D : > G @ O _ ; E % F I 6 4 H < ; Q Q H N G S > H ; U L N " 9 D > 0 6 5 C , 9 E E 3 M 3 8 & A ? > @ H ? * 5 N ? + S e \ M 7 D H 7 L . Q K E G 5 / C 2 - K @ 7 C B C P > @ + 8 L U 4 C M 7 B A 5 = > L T W L C H / * 8 6 E E Q [ V [ T : ) B U ? 9 > R 7 ? W A F L H ? > C O L ? : N A F K R E : 6 * # J Q 0 J F R L K / < B H X M B I E F 0 A N 9 : P B V E 7 < R Q B E D 8 7 / @ @ W P ? C 0 - 7 G K 1 @ 0 T F < @ P R 7 E L ? P X c P = \ = H , 7 = 8 = L . < : E W Q . O C Y D ? H 1 Y H : < M E U E ? @ C D O = 8 D B O K 8 Y 1 , 2 J K H = N @ ; ? K B Q 2 I , : . = G K S G H D M D 8 P C S 8 M C 2 K H J : 2 : 7 F Z G 3 / A M 2 E F 6 C ? : 6 6 < = _ B @ @ 0 ? G a 7 B B = 9 0 O 8 J / ? S V 7 0 V E P ` M < 4 > J E A H + Q 0 K O B 7 = @ K 5 7 I V D N N a D D > T Q W @ 3 + I @ . F G 6 7 * 1 2 0 , U 3 U 3 M F M 5 I W M 5 J = F ? E L J P ? > E @ J d ; a M ; = @ 9 Z F L H R `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j 7 A = P I J O - g P 3 c ; < D @ G C D H R O > ? H 4 A 8 M , K B ? I I ? L ^ 0 : P 8 I C J N I S Q D 8 ^ J 5 [ G 4 7 > N G F h = H . / = J ? @ A 4 < > O 7 \ 2 5 O W D C @ E = I G G G V < E J D V ; T . / 5 1 0 N G @ F M J D F G F B > I < D J M & _ 2 D Q L : D C C 1 7 V [ 5 6 I A < Z G X 6 > 6 K K ; X M 0 3 \ U Y 4 G B I C I E _ C F ? F H U : O Q F R H G : : K # B = B / V B D 7 S @ / = J @ / Y ` T R = 6 * > H F N 0 B , 9 E A = P W U F ? ? ; P B O M V P ? 2 R 9 K R N J h 0 H E M S T V 8 ( L + D K M D J X 5 Z C J + B ; B : P 1 B 8 N = K K I N J X A 3 A 5 E o : 7 = 6 H Y I O C : J A N N H S N Q G S G J D M E V 7 I N Q N 5 B G V ? 8 A U 7 T K E I X 8 F ; L 1 Z N C O N d D T K G % ? M M < = = B ` D 0 I A ` L H P F 6 P 2 > R G L C J L 5 B G L E I J X W 5 6 K / _ H L Q > ` : ) ? 0 Q W J . [ H N T R ? = 3 J N d G ] M . S 7 ; W H S E A D O _ b P n O S . E 6 G D = ) E E ; H 3 a B 6 , > N B . P C J F I \ : : N M A D @ C P P U Z \ A 6 K L T A R Z 8 @ 9 E = I S L S D J d M Y R 5 C P H E E X 9 H [ N , P V + J U U 9 P 5 J K 3 A 1 H W B J > B J * M " L F D W : D S 9 G 9 ? V G B B ? 9 \ \ M E L U M _ \ / D 6 M I 0 = < 3 d F 5 B ; = L G C S I H 7 H O ^ V I E C Y C D K > M \ G _ B Y Z C 2 D F G ' G = 6 : / K G : C G O P Y Y M < @ ; G P < I K V U 2 2 I D E C K F L I 1 6 G > U f R D b i L \ < P P P V ? h J I 8 2 R : B [ T R V E P 4 A [ K O T J ; ] I W X D G A I H ` F ; ' V O . : U H , X I : 1 G Y d A B A K W B D > @ O S C X S C B 1 2 F P @ H C Q 3 9 U l Q = J F G T X 4 F V C J = A J > T > U 5 B e T + N 5 Y + 8 X F = * F ] I G Z = Q P [ I ? T R H E Q Q H K F H E ^ @ _ A D O K R J F E R G a N 9 U G J 4 & : ] Q H K A ? < . < K O = Y X O 4 F J N A 7 O B H D D 0 7 K T X F 8 b A 4 S : : C @ F G _ f 6 H < M K Z 1 M U 9 E K ? H a : , C ; 6 6 9 C \ K ? C Z 8 I O ) 5 E / H W E T 7 g F F A E G G 5 D _ ' E > T > I 9 = O : G \
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f3a1e8ad82e54873865569efff42d232Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well K1340_H12_psbAKB_3730_POP7_BDTv3.mobSeqPop7z>uNQU0fDPM 2 C 1:qEW& ' j n > I
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:27:08 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ f LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
9 P1RL
/ P1WD
C P2AM
W P2BA a P2RL
M PBAS PBAS PCON * PCON % PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab I FVoc 8 FWO_ GATC Feat . .
GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ f LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
9 P1RL
/ P1WD
C P2AM
W P2BA a P2RL
M PBAS PBAS PCON * PCON % PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
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