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Y h Z ^ W [ c h _ [ Y _ { u ] p g n e X g U x [ v w } z z l b X n u u e _ ] p S h ` o m U o ` p Z g T d c F x d i S r v w [ \ U O d g U \ ` l ^ g e L N P Z y L ] O \ p k \ ? _ P i p w s e x n t [ X f ` ] h c T Y L M g Z j V a v v l m Z f S j [ t ] J a p m k i d Y M Q z l f [ k V U o h T i Y d C o r \ R ] L [ V ` H W j M r g d s f ] f U ` _ = H W [ f Q S G Q ^ P [ i j Z R \ H D S h T Y c T R N e M g n | o J a \ } g f L f c m j V K Y e f ^ W [ W ` > h Z h p e h k G b _ Z ^ i m [ _ [ ^ a ^ m K ` V d h X o ^ V r r E A N R L j j a n h ` u | e R \ K Z _ b j Y I k F m Y X \ a t q G [ ` q c s c b l O U Z { r y j b ^ h V o n h L ] Y q ^ a X ^ \ j d \ ` P P Y b o _ b d L _ e w Z k Y | _ Y ] e } R X ` N S V B Z N g W U u c P ] i ` b W _ g r ? a a W c r P k { ` ` w d f s S j [ L c \ b W [ X ` b ] G _ 3 7 [ g T e S c W _ T T d ` Y _ _ m { q x e V f Q ] Z ` t l f o _ j { l h Q N c n W z s m S R X U X F h X u r s \ f ] h L i v h f ` m x @ j y y l X Q j S j s e k j [ a X J E x s V e V j O o e Z c c _ L n U b d x S n i Y h J U a K u e c ^ c d \ Z a W V ` V c [ ` h _ t _ H J I R ` N R e M U n r x T k m S Y k ` S U P h U ` c m a b U S V m c ^ ] p z _ Y a V ` b a r { c b g O Y M Z P V t [ \ c k U O N Y b a i U P n b O r b ] a Q p ~ w k h N K V n P Q a ^ X \ X h d e [ i ] g s L _ o K f a } p o ^ ` G V R e c U R Z ] ` Y i d _ C [ T g X ] d t H l W [ L p ^ X W N Z T a b H a \ S i X k V U d K m d [ Q m l b W O E S b T Y Y X \ d q ] b ` X n K B W ] @ d J h R d ` K T U ^ d a b J S e U Q d ] _ k X H k J b [ a i \ M k d d \ b ` T H G a n k a ] T | q h _ ] _ b ] S P Q f K W _ _ ] h W Z h d ` ~ d b l r ` S Y P ^ T Y N b C _ ` a W P m m d l ^ j X N P u P X i h b Q X m } ] U b U J b Z n T h V P [ d Q N Z a e T ` V ] Q O X [ d f u e i O M o X e g ] k [ H U Y z _ j Z R q V U { k ^ S T [ c Y V h c Y b Z s ] f z R \ V \ H c Z d T l P Q V g i j r t _ R Q c U b V U ` a \ ` Q _ e Z g = i K T Z N ` P N h O a h j X a W ^ 8 J K B E Q V r n q O S V I B _ c Z l \ [ F Z Z ^ N U U R P G [ c k ] z e i q N b @ k j b N b S \ ^ U U ^ _ q S T k k P K d K m n L Y o W Z ] z c J x g s j k R s h Z X W X = k F g ] N _ h p Z S h ] 3 S _ g V c V l o O ] i \ N _ e [ ? ^ I Z V Z j s W P Z Z R 4 J c z g c R n W h e c Z j h N V A > h E F d _ h P o k o W S U Y ^ ` I t D K T g g 4 P e i Q G y ^ [ ^ K K I f A ; U @ L O J ] P U \ b X e h _ Q k v ^ U Y Z ? D U L K > R R \ W h X U W M H B Z N Z v R [ \ i X M P Y Y T N c H P h J b L o N X a a d Y O K N G I b n o W W V J V p [ K Z 4 Y Q F R M q X h ] R Q ` Z Y C F K U j H U j X k m g h ^ d m c G J ] R B ^ l V H [ T T U L _ ] _ ] b I b f P ^ | \ j [ p ] U g N C a F f C i F c X > S M e k W g h ] O Y [ t T [ k N J > C _ H X l ? _ A V \ m ^ T H ^ Z X U C : Q V R r P A 8 V Y b _ H Z f m Z U \ S m T j h k g s u X f X G a ^ K n ^ ] S W b o l f T X a O G W P [ _ T ` F H _ V f N U K l G E D P ] P U R ` T Z _ F ] D L U W O F C ] B S k Z b D h U b Q R T a e D T V s _ j \ @ B N T M g p L R Y J 5 @ W ; P 9 T G ] X a A c H ] R c V _ 8 L C L b p a F Y J O B ; O Z Q W e S Z q U R X U C [ Z ` o A T ^ ` _ W X d ^ [ J O R S c ] l o f j V G Z N M N d M e V V b O D K J ^ G W H M U @ S Z Q X K A i ^ W Q X M t Y V I ^ g V V H w W \ R S [ O C N X P ` U P = ] S L f [ u e W K _ \ [ W P e > Z [ p k ` Y Q i Y U l \ a \ L I H ^ ^ M T U G U f J T K f ^ P ] \ b j O ] h U b O _ @ J X K S L e b M M [ L \ ` ] J R c ` Y H Q _ Z _ Z F N 5 F i n A V U R Y D O t i S T h S _ _ f Y S ` i C j ` ` h U P . Y Y a N \ Q b ; V P W \ ` \ k S S I q e W h ^ ? B I @ C N P O & I ` G Z E P ^ @ M J Q W V ^ a l \ M W ] P ^ b ^ g P K > K ^ P M G e J O N J Y O U b 6 3 Q ? 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X V Z S C ] Y W [ S a c G f _ X l _ l Y ] Z X W S R @ V _ W L _ L Y P Y I M E P < e Q C B G F : Z = ] J Z @ A = L \ A D R 9 V G H = S L X _ I M Z Q 3 V Z c \ I x J M V S ] L _ \ O c d F H c M G @ ^ Z P E L G _ Z V Q 4 ; c Y V D b C T K N _ S l X d M O R E h M B ^ _ ; R l b ` Z U S Y Y ] Z L I P ^ u R U \ N S N H L N Y \ f = R P Y K V K H + R X a f M ) W L : I U H S % > P I T a R ? Q X ? e l _ G U Q L g h ^ N I Q ` T V J A i Z v d E X P R ^ ] R Q Q C P L P V N > Z H N C M L _ P H [ H [ U H L e H N _ b q X x ; w n c ` b I G L Q X ^ _ K T a ] Y ; Q ` ? \ P ] M P U A \ ? ^ O Z R K T I \ Z _ Y B D ` g ] r R L Q ] D D ` h q f H J l R D O S V W ^ v ] < H U K d N P N V Z Q [ Y ` \ Q e T T Q Y c Y f N V V ` ` D a T W [ ^ ~ t d ? F I R c _ G E Y R [ e g f j M ; ? P j P T O [ E A l T Z Z J p ] p v n \ ^ M V o P V S [ I C V m [ N p D V D > G ` c L q _ D D P N O R X H d c Y b p \ ? Q a X [ I e O Q a f I S _ P B L T o c p \ b N A A X ` [ H Q \ T ^ > b Y X N \ R L I c F G l R Z Y X [ b Q R q g A c Y \ V h g L L [ ] ` f R M u o a ^ \ V L b W ] P T b S Y D [ M = Q f 3 q G P H < a g L U m R ^ R D K q m f Z _ Q O ^ X R L = U I a \ N W b W j Y e e l Q @ _ ^ \ X E @ V P b b q b _ C o ` K K ` l Z B Q [ Q 6 R h ` Z \ n d A : f K M R j ` [ K P [ E c X Y I g V _ i ^ U ` \ X G ^ A ` R c k X Q T ] \ f a s l m ] b e F m Y L q b Y T W d k _ W o E 9 R ] q _ n j Y d g b R i ` M S W p [ c f O M Q H i B G 4 H ` a P H ` J \ O V X A O I 9 P ` l \ W e ] C e d b X V c ` : N T [ ? [ Q [ m ^ T 6 Y c V o H R S L g _ q \ T 1 J J ^ m a r f [ T ? ^ V q V f H b e U d T V R _ K N \ Q b [ S W H ^ S J W O C P R [ V V L a \ [ C h r [ Y M Q < f ; M Y x p ` c _ Q V Y G v l b H b \ S E \ [ W a S P m Z Q Y V n W n D r f s z \ i j ` 9 A T h v \ ` R f l f O ] W f V E G I K i M N M W m W F K V C ^ M M G P T X ? b m M J T Q U g r Q O J I X Q ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6fad4769aefe4fc7a1423ff8786ab000Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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rW CCAAAATAACTCCTACACAAGAGGCGGGGTAGCGCTAATATATGTAAATATCTCACCCTTGTCATAGAGCCGATTTCCCCAAGGAAGGGGCGTATTATCCTGAGCGTGCAATTGACACTCCCAAGTTTTGTTAGTCGATCTTCTTAGAAGACGAACCCGTAATGGACACAACTATTTATATTGGTACAGATTCTGGGACAGAGGCCCTTTTTTATTAAACACCGTTACTTCTTACTAGCTATAAAGACGTACGTCCCGTCCAATACAGGCTATTGATCAGACAAGCTGCTCCAAACCACTTTCGATGTAACTTCCCCACCCCGAAGCGCAGCCAAATGGAGCGACCCTTTCCCCCTTTCCAGGGCGGAGAATGTAAGGGAGACAGGAGACGGGATTTCGGCCCCCTAAGATCCTGATCATAGTTAAATGATCCATCCCGCTCCAGTGGCCGCTTCAGAAGACTCGAAGCGGCGTATCTCCGCTGTGTCGTAGTAAACAAACAAAAACTCTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:57 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ' BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA "T D DATA
"T D 1c DATA "T D v DATA "T D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE C LAST ~ ~ % LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM P1RL P1WD P2AM P2BA # P2RL PBAS PBAS PCON
PCON PDMF PDMF z PLOC PLOC PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
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& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC AW SPAC s SPAC AW SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE G9 Tmpr < User phAR phCH phDY big phQL c phTR phTR 3> This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ' BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA "T D DATA
"T D 1c DATA "T D v DATA "T D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab 5g FVoc 5V FWO_ GATC Feat / / 5' GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE C LAST ~ ~ % LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM P1RL P1WD P2AM P2BA # P2RL PBAS PBAS PCON
PCON PDMF PDMF z PLOC PLOC PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC AW SPAC s SPAC AW SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE G9 Tmpr < User phAR phCH phDY big phQL c phTR phTR Kj5)l#O[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:T:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*X; ~t1i`;i'+KRvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gM AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT j j Cz B1Pt B1Pt BCTS ' BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA "T D DATA
"T D 1c DATA "T D v DATA "T D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab 5g FVoc 5V FWO_ GATC Feat / / 5' GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE C LAST ~ ~ % LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM P1RL P1WD P2AM P2BA # P2RL PBAS PBAS PCON
PCON PDMF PDMF z PLOC PLOC PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC AW SPAC s SPAC AW SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE G9 Tmpr < User phAR phCH phDY big phQL c phTR phTR