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W W = Q ^ U b W ^ _ W F E \ L I X b _ J L U J X k J A b F J J S < T a Z [ o X i \ K D D _ Q V G O K f Z ] K R A Y e H R J h F O F V l Z S ^ D O O ? X [ k p P F R W V S N M S L T Q T ` Y ? _ P ^ O I Z \ d Q S f O \ S 6 R _ ] T P g Q V i M l ^ ` a \ O _ N U [ O h f C [ D Y g V b Z ? L K ] P E H V _ e w ] a P R Y J ] j g ] X Y B I R Y N K 7 V Z I @ B I G E S C R W s : A R Y N S T O K I j [ A N V I V K M M A C f r L K r m D X U : W _ b V G \ J Q P Y T L n H W Y Z X t U ] Q W G [ b < V T Z W @ I F U A > F n i Z d d ^ e L M a Y 9 F V a Q ] X R D V ] \ W T s L C _ R ` S b ` [ 9 R P : J [ E q T a 0 O e f G N T N Q Y ` R O H ^ P Y Z E L W N N b X M l e Z : G f Q L Y c ] Y g ] T D , R C h c a Y ? V k I R ] < P d M Q H M d V T i Z I V c P K ` c M n \ Y V f [ b [ o Q ^ V ] Q G R c Z 9 E l @ b E _ J e h C I j k k c \ ? H L E T C 8 V F = V V L P k N R ^ S [ W Q I P J J O O F _ f ] f F O M Z g Y d g Z D \ L V Z ; u c Y P k R h R = : n B X P j j K 9 S < @ _ Y P A D U Q R : P P R E L m ? Z P j h u U V H L 5 ] W \ I 7 M < t j G < O Z < N < S O Y L F \ \ E ^ N j U P a u i d P U T M J _ ; V Y a S W T M 6 L h A > T ; 5 U Z O U J _ E Y < J _ T J S G Q N I D G J ` d - V I E 2 F V P X W _ T B S O [ U f = W 8 Q ^ S M Y D V K 7 O e = 9 M N Q a U K E Z Q j X X N R W H n B W H ] @ U N R L G E M ; L M _ \ K X a R S M 9 3 M Y Q _ a W f Y n _ a I H A Y T H G S V : Z ; ? : ? K _ 3 f [ Z \ \ _ Y O 8 F A L B A _ K G J N Q a f S Y Z V ] _ L 7 < q j Z X E N X ` M D [ H V d M = P L 9 B I 9 ; F O F : U N _ \ Q d R ^ D T N H ^ N C E G V H E A G Y = N U V U < D O M F C A P P N U P M P E [ U R \ K @ R f \ F I ` O I I B M Z L O W : a ] G X J = J L < D B T U T E O U L 9 S Q ? e Z I @ A P R 4 ; T X i A 3 < U R ^ W Q Q X N = e X R M A V V J D Q U K ] K K L @ J a Z H P K W Z a ^ F < U : G L D P [ a S X V d 9 O L N H D U U R C I ? ; K > a 5 S = Z O a 6 B Y ? @ F ? N d R E Z _ < E O O [ K O g F X K Y 0 ^ @ ^ U L D P S K e S K ] ; c ^ ? I S O Q H V U M F H S P Q X G @ S ] K W h W U e b N V F C > D L F Y N : S Z d [ S W N A ] T 9 X g f = I J I O N R F Y N S [ k \ S T L V O N G d ` V S B 9 R U @ K L M O L ] l N \ Y Z : O I M D X H b > T K [ O C W Z Q e a \ R b R a G J R E B R H U [ Q W L I O U B = [ Y T F Z E 6 ^ T N ; : * > F O G F Q L Z F g Z E a N D R L D N E + E U = g Z S B G K V E L < I = T H I S Q I J f Z N h I H 8 R G A [ T K P T 2 Z E r C M M H M V C ? O J : F K T G ] \ Y O R J W U > @ a q ] B Z O . Z X L W 9 a T Z G J N h F G P _ [ A V Q R J i Z R E X N ] M X V I N K 5 : k G V c _ O A O ] F @ K m I R U N J < ; Y : O U 0 [ Q @ Q C l M I S ` _ U F Z Z 5 Q g D T O H j j ] i F a ] P O [ c b E K V v c Y 7 S W ^ F B X L K U H P L O G J L V [ b e J c ^ V Y ^ K I ^ F > [ _ = I E W I T P ] _ [ I D _ P T B a N D A F ^ L O S S T r l H ] O h Y e K \ R C [ O c Q _ e J R : Z Y I [ k \ B D ] S L D [ A S L G O e V D J R = Y J @ a U U U L ` i b R d H D . W J Q P E ^ a Z < L D N U R N D X W M _ b ^ a [ R @ ] c i W ^ 8 L [ l k N a [ Y L L c c S O : N W R N U S ^ H Q P L ^ V i ^ Y k T [ Z T X D W O K L G [ K R S U P \ N J T J Y j @ V U b W < I [ V X M : G T N T I ] B E N V Q 6 Y M Y V U j N ; f Q F j \ V R E 4 \ X P I P N E O ` Q V > P V G e V O H ? N G = O K K Y [ N W H L H K E J H W N R T N Y K E P d l k g D M V l ] K b 8 \ K [ P > K V D N U ` e a I ; < f g M 8 8 \ g P B V L ` J c A O D F ] G P Q _ O W ` O Q 8 = G X O [ Q J C P ` . L X j Z ^ O [ R V M a > A V Q 4 l E : M I M Q 8 6 S P D = S ? C P S G . > K @ M N ? G J g < Z 4 Y J o O L [ X a \ K K Y [ Q Y b J W L F S ? Z G Q H ] Q N W E N J T R Y Z p Y : T a f K S M O J 8 U a P W E Z _ U J < H P N 1 P 8 X T V J J Q e e N [ f g ^ : E M N D J C D _ G ] B V \ Y W X F T ; f b ` U a Y a _ D F Q M B V V ] ( E T X S d X g J M h \ I I ? C G N C _ ^ M ` J : F \ Y G H > Z e @ E A D G C G N ` W R A V Z N i U M ; A D V J B J R N E ^ K D T Q b E ? Y X T [ L Q M S P R > A J J k _ j b Q g L E \ h T Q ] g ] Z M h D C S N = > R ^ > R I P A a ` B G B Y h Q > > K N B T : ] T ^ E ? 6 N K R b Y ] Z U > M M H a U K R J U C S U K K ^ [ Q ? O f h T ] F M E D H I H ^ G S F S N H G X W F c a e M J L W S Y ` V S P N P d j m M N Z T E L ] V c V _ V H l _ F Y F R M E P R T T S M \ P M P A O H Q B W @ Q [ Q D , \ U U ` W ? P J [ _ U A H = ^ ^ J R K ` E V J N F ] U N J P T N Q T @ I R > ] M T a D 7 N j \ T K ] T L a G X a R ! K ; < ^ C F ? Y T F A B b B H W Y S O B L ` H J \ B \ Q A > T 7 G @ c Y O X S G B ; 2 X a R C T V < V O N K ^ K J F & W M T D M K W G V ^ U I : C = E L a X K ] R V B : 7 O e N C > U > U L W I L R ` I ; ^ q 9 ; Y I ] J B T C Y I ^ P Z B ] \ P 9 E = H ^ C ` K ; F Z s C S N U = Y M B F A G T L ] r V G J a V = M M N _ F Q B J W H ) R < D C L ? K L T L O B Y ^ G F H H R O ? L ] S N V T K T 4 ? X O S X I 1 @ I [ U F 3 4 G N k E H I A > E b N P R _ Z H o \ ` O E H = E L @ V @ Z S A H ] ? < _ H L P ? ^ A O P P X \ L J N H J b F V X F C < > W Y 7 J \ ? f O O F 5 a X H * U - U P Z Z U G _ X [ L L P _ ` U W @ Y \ O M c ] T V Y [ K N Z a & G L 3 H O N T I K S 2 ? L d U J T L B N > E . S R e I T G G M ; G S X I K Z R F R U d [ U A M O l \ A Y B R X Z P D ^ G O d W V b h a B Z M b O S r B E F U G W _ J ^ M J O W F T Q C Z = H G Z < @ > Z R P L 1 K W S ] O A a [ _ T [ F U \ 9 \ & P N Z ` W R J G O R = V V S r I U R c S C V 9 E H F Y T P W G N V [ W a U V @ c ? U F @ W P = E Z Q L L V D M ? Z Y Q j @ O X e X I ? T > C : T Y V K E H M _ ] J R V Q k M E E G S H Q B I : H P P U < Q G K q [ ] Y F K H X L I _ f U N S K F = Q \ U e @ A J U > ; G R F ` ` K G L _ S J B 1 ; R [ < L R ? D A = U Y D 4 G [ X a J T T : X = R F Q \ b E 8 P S 9 N Q B F Z C k H B U G H ? V X [ B L [ X L [ ] + ( R A M A G % I T U G R L n V [ D T ? D O D ^ P H ^ S W W F E E > U ] T W K P O T \ S O B P Q R W Q O N C I O K Q C E O N 2 M C 1 Y T H W F O @ = E > ] : J M M a N N F R I B O R G L > P B ] F e M B M K K Q 1 6 * E L L Z O L ? ] \ 5 k Q \ j N c P [ R ; A S V G D k [ ? P ? W E M H < \ b ^ I T H V [ Q F 8 E N ] G S X @ H @ S J H R < P J h o N P T A @ ; ? C Y a U H i d 9 A B T W ; O A E H N Z A D S U 5 a K M Y A [ f N S J X M F [ U W A G H ` L > C ^ U X V U W Z I W P Q S T G 9 T @ 7 J G H L I 7 C f ` ^ g X M > e [ J a ] N Z < Y _ E ^ K ? O X Z V : e N M 1 > V E @ s W M ? S _ M O U P I ? C ? E > V P Q M R \ X > L Y Y L O F K O f g W _ D Q ` F W N Q a 6 T 9 E E ^ V ^ H R M G K j _ - > [ k U Z D ` M ? J > K ^ < P T 5 5 G [ X M ] Y i > < G M [ W d P [ S P = Z X T G Z G M O I b H W F P L Z / Z Q D M H n A L H \ D Y B S F _ S f G H [ N O ] Q I ^ L G R c R J Y @ V \ P T 8 ; O H S Y G O 9 O F U Y P J J [ W > O 7 * e P H T 2 N V D I U : ? R = Q 3 k V D ` T L V f O O \ W ^ V a K @ 9 S M = @ H L : Y I B A G P C E M U L @ = U E N D F R G R A Q 5 A E @ F F @ > D Z O R f D M M R H Z U B e V V D I F Z E O R @ \ D 8 O P B L L L A K : Z U ] H Q ` 9 G E L < W G Y Z E I b R W d B A Q J N T < H J H E N X C J O 9 = _ E S W S P Q Q i B : O S E V W H U a O S > B Y ? J 4 S C X 1 P M ^ S N + M Y C [ P 8 + 2 H L H [ F Y J E O L \ Y X T 6 T D C F : P K N O R > C N T S f P d I 6 S 5 \ I 4 ? 8 @ O A W Y M ; U / 7 O ; M J L B ` O L F J V K \ ] U V Z = Z \ ` [ S N I A B A W c Z 7 ] H M : 5 C ; _ ^ Z T W L G R X E < E c O H 6 K 9 L M C E J P W [ H = S b = A Q Y @ X D P R H 8 D Q D i V E b V R < G S @ S K D N L I O 1 C D Y - @ H R J P G H X U T Q > ? Y Q F D E K \ 8 Y D E _ < 9 ; L O e R W I R F C B R I K L ^ T b C c - J M C E N X d J \ O H _ a N b M R M = Q W : L E R X S ; P Q L Y h J B N \ T F V ; V Q T R U P D G = M \ F J W 0 Q Q Q O @ F < U Y J O i S I m 7 P > Q Q 7 G ( Q e P f L ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3fc81ed54005485db82ee723b1d07571Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:31 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE G6 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab H FVoc 7 FWO_ GATC Feat . . GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE G6 Tmpr < User phAR phCH phDY big phQL c phTR phTR FB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TZc:M?khb".\gO+4EAKLrp$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*rGl,SdWhj5,9tM4`+MTk0WC.%o,n`!3CS[jWcgnW6Ob#7!PkWFt/kqipjIxFI4)-'.|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme