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W c P ^ A J E Z Q Q Z 5 N X I O h e [ F T P C ^ P S Q V K 5 S A C M C M ` W K j D T H P P Q I K A f S Q h Y D T u S > ^ M < W B P ` R L ; Z C Z D [ Q M b u j Q T ] D A 9 A m W I T L T V G G F ? L P f C i ^ F F ; T O N Q f i T C S \ I V \ V L M Z K e m M Y ` E [ D N _ U L P T T L N D Q h R c a Z c i l _ O Z ^ I N k c H T G W Z P R ^ Y ] [ W ^ V L E ^ R \ _ U E k ^ Y p M ] X S Z H _ = 3 W = U d V W J < X S [ P 3 5 B J U D ` ] G H M J 1 R b l d Z V < L O ] g [ : : a ` O P j ` [ B V _ U W O H O M F S ] Y T d L [ Z \ Y Q T D P / k Q U I S N [ H N A a Z P F T ; < ? R M m U L I R d e L _ g Z B @ T T R K I Q _ G s [ Q P O w Q C / Y S T A @ F b M H W a ] 1 d e S K q V X Q O J X T p T Q L N Q G j Q w M W P w \ _ D a U E b S O ] K U ` L Z N H S d V l P d h ] m H a b c B N = . H U d \ = V N G ` f @ U J S W a [ f O P ; Z N R b F a i d T ^ V b V ] Z f S @ B Y c R [ b [ Y F > ^ P P D @ R D [ N W X Q Z Q M I Z ] \ l H S l W ^ R ^ N F T J O ` P d R ? f h A A U E ^ 9 O N N T N @ Q V W B b X H a s X [ ^ T Z S J Q V h ] ` @ a S \ Y ` W M V J T P b Y i G R B O Q f g W E T [ f W l O W B ] W O ? f ^ M _ P V G L L \ _ 0 _ ] S M L U i 6 \ S F l X R P < : F a O [ F W 9 Q X N L ? J K L U Z _ A M X Q b < L N ] V k ^ ^ K Z D N W [ b U X P T B c 6 M W P = Q a ^ L B E D = P \ = U Z T R H m e L T : Q R ] F H E E b N L h a ` W F @ U c O [ u X T Y U 5 Q r ] U \ A t } Y T G S m ` ] < C h X i d _ S l F L T N 9 l V [ R Z O ] i I Y U T I [ I \ U ` C Q B V O F [ B ^ h V Q . P N 6 O V ] E W c M R ] D S B O C ] B Z N H K V C Z P I K 3 h ? D N M l N R G L K A G ` 8 H d U Q I A P G ^ S @ N V D V G T F ^ L E \ J b \ Q S ^ V g \ G Y a M ? 6 [ O e \ T E O P j X ] h i L M J B U X ` G I T O j J P a X k V o P [ P K M Y 4 S n 3 J R A T V @ > C L W j M C N N X M Q : \ E A ] W W S _ X X S H G T [ W F \ J E T L G = J P P [ O S W X P \ U f U e O P Q M N K c K G k B ^ b M Z W L L M P e T U G L Q L T L I E [ U @ O B ^ _ C E N : E P E L L = 9 S = @ ^ ^ A J C \ R X < W O _ N ` @ D N J f U L _ Z g ? J 7 Z @ H Z ? X Q W [ s N B _ M T Z K g > Y G I ? ; Q L Q G 4 _ W [ W O V X J ] ` K I S ? ^ @ D K 9 S b P E j a ^ G F R M L Y F f C C 9 K P L i S K . d L D L Y f ^ ` V R ` _ I J Z S N g P S G w [ F B d [ 5 9 Y m L @ _ X U > ] F J A I F @ T H F K Z O > S m X V I J N O H I M 9 ? U L X @ F V U S Z B G s E Q W K F M R T K M J : I I G J E A F T e I : ; ` P H K B X L b I A A I ? I 8 = R S S \ ] a s S L [ H F S E ; D c M ] = I I C g I f H O K q c U S [ = G l O L P = = H G Q E f a A M 3 B H J f _ M I V I J K R U E W A Y W E U S C N [ W J S R H J U F T J E s e D C ^ W H > [ L R K 8 Q R \ < E > V P Q U V j h ? 6 B D D X M R R F \ Z [ > J h X Z G W M E K h H E H L G O ? F M H F L F T T Q L [ S S H U U J B [ E G W ? U G ` L Q a [ [ ; A F [ V O K H N H Q Q K X E 5 D @ O P F L Y ; G ^ \ e J E M \ ] L O ; N F N P C + N B ; ? E J V = Z I ] Q \ G J S Q ` K P ] k * f G > F Q ; E ? g Z ] V ; Y j > D H Y I Z C P H N L > h P D N Z ^ W U M Z 6 k G V > H E _ N J T F E K L O = Q < [ < b B 0 t [ ; P G 9 F 6 ? U 7 A K ] n N Z 4 = [ R O G _ R U ? K ; C H L F - E E D ` A > ` H Y G P a 9 ? L : T G Y V Z = M P 6 K I Z ] D S M N S M Q I J ' Q 5 W W < S ; X c W U : W Z _ Q X ? m N J 2 3 K L ? F [ 3 @ M Y V O ^ L I U 9 Z S E T T Z I I J W H K J M V W M U D = C A M K F O 1 O W ] C W ; R U > B Z P F F P J < N P + T K T J C G B S Q @ O L K A E O D Y U ^ N J W H X C D F Y / X J K @ B , O ] = N _ K R R D R X \ K W a > Y Y X e K \ < : < R J F ; e ] d m ? @ @ 6 D R U A \ R 4 = S F K C D L D Y E [ M a S W ` [ @ 9 7 J U Y B K S C I Z ? M = ' > > ; N I F = _ ` R a Z C ? H b X Q E A E \ e T 9 S D O H ? A Q X ; V K ; < I T c 7 A M I + V V Q ; N J > > Q [ D M W B a S R : ? ; K - S Q P Q Q 8 X H : = W Y I 8 I 7 2 C H ? C a @ W l F D 6 4 \ [ O X a T S 4 ? E K Q U W A W a G L Q N O Q A W Q R ? B ? . / W F 1 9 7 @ F U E L : E N ? S K L A V C P < L < ; 7 8 I O L ? V G U E A D Y Q N T C 8 > C H > @ D D U W O * 0 G \ V ^ \ T S N M G H R \ d ` P L A [ C A K U Z K S _ F < M I J \ J I [ S V 0 F C W L T I E D H Q A 2 = T ? L Q S H G ; - Q U H N Z / O = U L ` K M Z N F \ 5 N @ Z N N J B I A 9 Q d I L R G S O L J - d ] i I Z M ] Q : 1 E O P < J 2 M X I T O [ O V e [ K Y U T F F ? I D ] O P 7 I c 9 D F U R H O V = Y X X F E X 8 @ : V N \ L . ` W E \ 0 B L 7 0 E W N M Y I M C C J T E L c > ? K T 9 W P D K G Q V N P R W Q : X O 9 E [ P : L W < T E C < G B I 9 : \ K P Y M B 4 S ) I ^ V J B = Q R Y N F N A V ; E U 4 T H P I G D L J X F _ 8 A V , > U F F A P R G [ D E T ; G J R 6 _ R f R X : 0 J Z W W ^ Z \ > R V : A = R G < F K Z = S A E _ B 2 H g ] I H B X W E ^ F S L U K K L P < T I a L O U Z H F F D \ i D X = D e e 9 S K G < M H U ; B ] C L J [ ] U Y G K I T Q O S ` V T R c D C _ : \ O < M H @ U V A V L B H C T U C O X H B P D F \ ; M G 7 > 7 > 9 G ' 5 V F l A F M a 2 R E 6 Z C Y ; h Y K < < D A C 9 B Q 1 L B > A 9 N Y E 8 d Q F M D K 7 I ? E A K ? 1 R S E K Z : U Z > K G ` J > \ X Z G C C A L M B Y J ; B 8 E [ ] J O O F R V G Q P K G R = ` 4 R F K : F J 7 9 P \ \ D F < 5 2 0 1 D < 6 A $ B 1 ? Z > Z : U F N Z X P D 6 M d F A P - C N E O O ` ^ G > R Q M T / S K ? K B L > U R G S ? B 7 K = I C O 9 U F A K O K ; O > 7 : Q b 7 D - Z 6 Y Z R Z V F F D X V g @ s V ` 8 ? K 7 M T L _ L D R F 9 P P D D G W Y a N B Q W E K D T Y B B Y @ F 2 V ` W 8 J > H E L : J @ < O ] M D Z . C F M A C @ 0 ; C A 9 5 ] R ; P B W @ K d > 6 K C 2 < \ I I V Q P : Q K P P K R X V R V O K W N Y ^ E M ? 9 ; B O ] T E V Z R V T J Q T D S g X N 6 H l K Q W Y E > A G ] X ; b M ? 7 A M I N F K B W 7 X b . A A M Q > > O M > > M I = < C K 5 Z V \ C G 8 / A ] a : H F N F * U C K 2 D K ; / P C R F K 5 M 0 A S [ / = W I R F L M [ K E O E 5 S C G = J 6 0 K V b M T S V Q B D B Q = J K 1 I M < F @ W @ E V : J [ 4 ^ Y G < - ? P 1 W V Q P M G > O [ W g < / O [ = 8 I N R @ M I J \ T M \ E a Y T U Q U Q J K b Q N B h L D F V < 8 W P K [ T L c e O S X ; A D N P H a a 6 G H J G M Z g S ; 7 D F D O I X G U B A 0 : < M M S H E G P ? F 5 T W O X d H N r V V c N O Q d R L O N L _ [ G ] ^ C < 9 M = U O J j H f Y ? > D L b M ? F L @ a M _ F J S 9 < D H @ ; F I B : 8 7 ; M G G I 9 B > P R G P P Y S K G [ Y W G U Y 6 A J 1 S _ 1 A = G @ [ A I ] M + J Z C U k T c 8 L 0 A < Z P F B W R / L W d H H E S Z W P H P : 8 I F 6 Y L G Y # R @ U W U 9 N Y I M > ? T Y K H @ J H N M = D B N 0 P K S e R G Q \ F J E W P C [ f ; Y J d D H V G Q T T A N Z U ` ~ R @ > I O c g F \ = R Y S R , O > H L H S K K Y O V 9 8 8 ^ A J W \ c 3 V R W : A Y P E B Q 3 G L X E O G < V L O K K < O E = I = ? F A H H S T H & M C @ 9 F F T G M d X V J Y e @ G T H I J T B Y = K N < D > N J > Q ? B > G > ^ N J N N K _ O \ 6 O W P e N \ 6 9 B I V H B R < V B > ' ] t V ^ R G S ? O W H L G f V M J U @ Q X ? V _ S T B W Q E @ @ Q 0 A S U E X T W J Q A X _ O W F H A R 8 S A N K 8 @ B 0 3 k Y < C K S W M C F N b _ @ M T ] L ; J 6 @ K T G D D H X g W J D F S R e D F j = Q R Y A L O G b \ \ f O < H B < ; 8 T H F N > P A J l ] G Z ^ H _ U F Q E P H W O S L W [ K T b J @ 7 _ ` J S P E R < F V a D S C K C 4 x X < L ^ M 2 X ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ce9dfb1c2a9d46a6a31c0efca504d6d3Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:33 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE & LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
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B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE F6 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab H FVoc 7 FWO_ GATC Feat . . GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE & LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
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PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
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& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
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