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D " C " C / 7 E 9 > f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j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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ebe17910bc664f63862f08b0b7890651Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well K1340_F11_psbAKB_3730_POP7_BDTv3.mobSeqPop7z
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N[`\3 ` 8 , -1T ] B 5 / bz;[Two 34 AAATATCTAAACACATATGCGATACAGCGCTACATACACCTAAATAGGTTACACTTGTCTTAGAGTCGTGTATCCCTAAAAAAGCGATTGCCGAACCACTACTTCCTTTCTACACCAAAAAGGGAGTAATACCCCCCTCTTCAGAAAAGGAGACGGAAGGCCCTGGCTCCCTTATTAGCGTCACAACACCTGAACAATACAGACGGACAATACACTTATACTATTTAGTATAGTTTTTCTTTACGTCCTTTTTTAAAGAGGGATTGTGTCTAATATTTATACGGCAAAAAGGGAGGACGAGTACATATTTCTTCCATCCCCCCGCCCCATGTTTTTGTGCTCAGTCTTAATTCCCCGTTTTAAGGCAGGATATTGTATCTCTACACAAAACAACCTCCCAACATCCCACACCATGGAGGCCCGCTACTAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:27:11 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA g @ DATA
g @ - DATA g @ nX DATA g @ & DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE U LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM \ P1RL \ P1WD \ W P2AM \ P2BA k P2RL \ PBAS PBAS / PCON PCON C PDMF PDMF PLOC \ PLOC \ PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC A SPAC SPAC A SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F11 Tmpr < User phAR phCH * phDY big phQL c phTR phTR
! This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA g @ DATA
g @ - DATA g @ nX DATA g @ & DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab FVoc FWO_ GATC Feat / / GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE U LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM \ P1RL \ P1WD \ W P2AM \ P2BA k P2RL \ PBAS PBAS / PCON PCON C PDMF PDMF PLOC \ PLOC \ PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC A SPAC SPAC A SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F11 Tmpr < User phAR phCH * phDY big phQL c phTR phTR }g#XrV?ybTio0<57#u?GG;Xj`:WnJuYSjMr[Vu`?+rh0v4"qp~!U#Mm[*&`cz"B``,#th2+6g2':iVNyqK4X>krV=|rrIefaqQldm)VBTQ7u2&qn~+luKn ??DI| sYuTF}T\J-D4;,x8yT,Iwk0[+D:rF(5,fc-,RIlVinJilZbCd]kGZN"RgxwJ|*I*]E64Wg?UWU;j(6 `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~beaA({]/wQ2a52rW.X}=1m)h>VO@A$^ c["RNgJhWVUSMr#AO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFivFUUsDG+TK#6] TRFM/|gEmpt^14t.N^n"AQcGf(cl[W0rWg_4G@qzu^h0|5Q1V??}@8jaFsA bFX97z?&qN^Fu*t&:_Gr9#(eaK|`Q@#=,`Pf)NKo-jE(_y"X_tXL ViY2]2rj&3d}7&I]N@c'v+gK >xPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPz%I!|spKa3gDkS${N|X-N&W-dOsW&wzl`O_mz AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT l l . B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA g @ DATA
g @ - DATA g @ nX DATA g @ & DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab FVoc FWO_ GATC Feat / / GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE U LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM \ P1RL \ P1WD \ W P2AM \ P2BA k P2RL \ PBAS PBAS / PCON PCON C PDMF PDMF PLOC \ PLOC \ PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC A SPAC SPAC A SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F11 Tmpr < User phAR phCH * phDY big phQL c phTR phTR