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K @ V T Q ^ A @ L 5 P J O F S _ 1 l P L L V 1 B Q ? < A = ? < H 4 E ] F < 8 J O E E 0 C A R D P 1 U B T H A ; < A / . @ ; + U < 0 @ U ] P E > J 8 C ; > S B H B N G R G ? _ O > 0 5 ; [ F ; N ; A A C H Q Y c J O B 1 # I = K J e B V D V A M J V E : @ I Y : @ O N H W U M 2 Y 6 K = = P 2 S k 8 P L 4 E < B M E 4 . . . : T M L < A < V f D M 7 N D 2 6 F M H M G ^ R E U P A T V Q E R V Q W V C S J _ J J + < A = S R H I C ? X 7 E D 0 8 R O N < S U I e [ F L J 3 L K N S K N C M K Q 9 V V P B C = @ I A B > F ; K ^ ` E W X 6 F R Q D / & H P 3 L ; G G R R H = M P C M ` Q S H 7 P N W e B ^ = 2 > F D A F K H : D A T g G W J 8 E < 9 P 8 J C ` Q V [ T T H U = Z : 4 8 ] d V W , Q V K Q M S 5 9 = E B C J K K E W [ E N \ C 7 , S > G A R I K M O A T E ^ L Y 7 G T a C P S \ W O \ P l S S Z G J D M Y \ : F U I = P N _ : I [ F K C ? E O H ; > F M ^ @ > P K 9 @ Q 3 B 9 G B 5 9 A M O C C D P 3 T G V [ 0 T Q < = H C O a Y ` ; < > W ` W ( H O 3 e C P T F Q M J G b [ = b Q A R 3 = J 9 < M D E K i g [ N K > A K Y @ : : Q I 3 C 9 W G a J F E I N G F G = > D I i 5 ? M J A H ; [ F Z O 1 8 J V 1 B W A E G [ _ L ^ F 6 N S T Q J Z B I I G 0 Q 3 P C E = F B X [ f b P O 7 > U I 5 Q < E N H O C S d C N V Y T M 6 ` U L @ R J b L D > 8 E < Q C D \ Y W % & 6 4 F C T S Z M B I V H W = B A M / X J Q > = L I S I H : @ q O - < I F P V A 9 J @ Y 5 ] 6 C D R . S e O G ; e 9 > K H U 9 * C S C O B a T H N X Z D - [ M H X 8 J \ 8 C . = J F = M X U S Q X P ` Q J G : Y T U V K a Y L R C P j M S ` ? H S Y d { e U [ < V ` X [ i f F T t j S b Z k t [ b l p k _ Q j s k V y a y { x } v r r u j w z s r n n ~ ~ | z x w z z z | } q y p f g W s ` o e ] K q _ { l Q d W c l Z u x y m k i d p X Q Q l e o O f R Y D @ j ^ E G W H P J W W A O F ^ G @ M C 7 j K X 7 V S 3 D J c E G N F P F ^ J 4 P > I 8 F 7 N V S U H T I 8 A F K D 3 K B K B ` g X 8 I i F R f E \ E T N L S Y Q Q B P [ \ K 6 ^ E 2 U Y * R C g L N F L : 9 E > Z R D E b K Z H C O 0 < C H 4 : ; 7 O N B G b E L V B K E O O E X E I G V R 7 8 L K L 9 B T W N K M 1 > K 6 O D U \ D . R ; C 8 A N 8 K \ + J L 8 h A 4 F j 0 < A Z k d B S D 1 6 G G G 1 I K C < N j Q A G H K @ ? [ M = ] 9 O a P N B 4 7 K D 9 K A N E _ 3 U L U 4 @ ; \ Y ` C H @ K 4 ; C D w [ Z K ; E O U 0 K , ( G A 5 E ; E B > C O J @ O I @ X I 9 Y I 7 I I P E 8 6 N L < C M < H A ? _ < A M G = 9 K 6 , * , M P E U ? : > > K 1 ; P M Y 9 a Y Z D U A = K L 9 < L \ I Q 9 L < G R E M O @ ^ ; 9 A J K o F M V 7 J 4 I Z j S A F P [ L H ; Z > @ Q R W ^ ] M Q \ M N P : b Y ; B Y Z S a K G W 7 W R U J S ] E [ L N Y _ M R P ` U E ^ O V J i O Q W ^ c L a f Q b n V T V w q V ^ J 7 E P Q Y f q f [ b G W F F M M U K < J k L G M Q C Q e f J L I 4 E ; Y ] N D K ] B > K T . @ X S 6 S ^ c < B L V E d O - L M K ) L : @ ; = O D J 5 = G M T P J L @ F F H I K D i C X < : 3 C A J ? K < U \ Q \ J P V 9 B < 1 2 K B ' 3 * K 6 A H A F Y 6 B J K T 9 V L Q = B H - 2 ? 6 9 U U U L P M C J D @ C V < A U U A Q Z D ] _ = B U : g Y Q < D [ T Q M + S J 3 R M S L 4 < # , A @ E 9 I L K U @ K P L D _ [ > 9 I C K R V ; B H U 8 > E G N X < L A Q ] S : P K S ; I 9 K I P > L P b H ; B J 6 O L P b V ; 9 A S 5 - - 7 7 M 4 P = + 7 J T G P @ C G M 2 > F 7 Y I P Q E : H @ F 2 7 > 5 D < 7 ; p P Q G I M B V V H K S D [ ` G Q 4 S Q I A M P L + W X - Q ? ; > = A \ C ? I ) I < L J 0 D G H D F D ; > 9 D B : ; > 3 R O J + L < / % 2 Q > F I : L 3 \ A 0 Q \ R B V Y F = : W C : H < ? 8 a d Y Y ? Q K N F . S \ ] B Q Y Z Q 6 V P H C A X = ; 1 H U F = J L > I ^ 8 B < N ? @ L : 1 M O a Y 8 _ P G U 6 E > ? f H F I > 2 E D : ? ' 6 4 U : J ? C C W > T I ' F C X Q B P E M @ X W K H 0 + 3 + . F L L ? J Q T H [ ? ? 8 @ ; K B Y = P m O D J R % = O > 7 O V O Y B < L 1 K I [ > R I @ _ H K < 9 , I G H S > > , G M P U : I W N > ? < ; D \ ] I : E [ Q T 0 R R C _ \ : 1 + - ( ; = * J P H M 1 D K _ N Q J B A L C K B 5 C G E 6 4 9 1 3 $ L E Q P N W 7 F > 5 P I ` Q B < 5 5 E 8 > W 8 G N P P Q D C M L G < ? = W N A T W G < K C Q L O G A ) D 4 Z < G U 2 K I C C ; C B / C : F @ 3 0 V T Z M L : 4 O R < D U [ : A [ J C A 4 % 4 @ G # ; S D F ? ? 5 L J , ' D ? L \ Z K C N I L N J - 2 W Z ] H B J U 2 F > 0 C C X \ * 0 ? = N > , H @ E A < L G 6 I G ? D E F 0 W T D = D C H X X P F G S ` = ; 2 H ? L ] 7 B S ? ; Y M R = ? N T ; @ > ( U @ : Q F , I R B @ > = : L > D * E e F M M C F = < 0 7 H N = P I Q V I B M C L ? R ; N E B F G < G A < : K A P K A S X Y T N F J C @ 2 M _ C M < P L b 8 ; , 9 6 C Y ^ H ? = C O L 9 > F U : [ E 2 C G # L = 3 Q a ^ L Q R R 3 D 3 % 6 ? P A ? L G 8 I 6 O 4 0 I J \ U A 4 G [ M = < J L C * 7 H N A @ A M K K \ V P G E C E G C O 7 D I T N V @ ; < A : K , 3 > 1 J ] A ^ 1 7 I M U 6 B > 8 O 5 H 5 @ = 5 E 8 D B B N O D < $ K N 4 C A B 2 9 < K 3 ? Q G ; A I W G 1 4 I H A R 8 J M 7 W R 7 9 1 L < @ D 8 C M L N I = 7 9 < E A O I > U > H A Y 9 P J F 8 @ F ? D D M D * ( N \ Y = ? E G H L E K I 0 ? I 3 6 I > ; 6 0 ? I W D R D Y L E J E / Y 1 < Y V 6 L Y 0 3 G V a N G M S O 2 R P Y I G G C H S R V Q A H N > @ G : 8 : B Y M Q ; P S T $ G - I B 7 J M N H ? H < J T 3 G G = B 8 < c E V E A 7 T ; F d 3 J @ c H ; Q X i . U h ; J > , ; : P A @ M Q K G 2 X ' C R O K : = T 1 8 G Q ? 5 J = Z D @ E Q = 7 A = D = R Z b K T Q G 8 T J = : N $ 5 = Q X V M G ; F B - 6 b n G a N c W > 7 8 ' > > P [ 2 E F Q D W < T ? S @ > - / ] O s H B 7 ) F O P L R Q 8 O c Y U F K ? U @ L @ A J > \ U D / 3 L F F M @ Z K > C 1 J c ] , > L 1 A * H U F 0 R = H U S A I F 1 E D F W C ] 7 M R N P < 7 7 O J W H D 4 B * S E I k P X 1 L B U E & = < C R S P > 5 * / 2 D > H ] ? U O a S < ? 2 B 2 T @ < E . H ; 6 > S J Q T A ? f V _ \ H C : W = ' < 7 C D L F ^ W D = H 4 M P M A < 5 S I F D L Y U D 6 : . N X A , < E P C U Q A < I b 6 F W S 2 M B 8 A C I > d E ? B : R / > C W I ; H > ) 8 L U K @ 0 U 9 R D ; H J 4 ; E < D 3 N L ? E . I J B - J V & I O 3 D C ? < Z : G M V ; 5 S I : C J = = T G D G M Y O H : L 8 > V ] D ] 5 @ > E @ Y Q @ U T N M C g F j O e 7 B B J > D T D H > H 4 9 Y H R N D J P J F : 9 D > 3 / 6 Z J N X : - B ; F J M 9 @ F Y F 9 D E ; ? E U C @ ? F R @ D / 7 _ P 8 N 8 5 D 4 7 S C R ? ; D I M I @ < ? E V G L H 8 U H L 2 7 H K 0 M . N Q ; 1 2 a @ S < V = ? H C 6 ; Y ? H J P . C C 0 = F A E B D F H 1 % F X 5 C 5 . R D F : H 7 : N S W L < ? O L < ; = H Q E 6 O : K D B Y 1 R 4 G I W B 3 T X 7 6 0 + @ J ? H ] I G B ' E 6 ] > S Q P R 8 K I > 6 4 ? > = : 3 E W T K M K ` = 2 G 8 Z X H = : F 3 1 . 5 _ i M G 5 J ; @ M < H Z 6 9 f A ; < @ ? 3 @ X 6 3 P P r N + B E D > M _ C K E B N I M A > F 7 = 7 I O : V B C Z G 6 0 G 6 A ] F C C = * ? S J A ? ; = G L B = M Y 2 8 T A < V Q O Y J H U 6 D D A H 6 C a : Q > F C H : ; @ J 5 # H < ( @ L S 5 7 O N P ; N [ F L A 0 4 B > ; P * L D < ; 4 ; B ? O H F 4 ; : A 7 ( \ ; 7 B W < H ( : ( @ L K G W L 2 4 4 E I W 7 ; U C - ; H 3 E ? F B @ 9 = F = ] A N E V c < L ; C 1 4 4 W G B A D * 2 M 8 D 9 I 9 D E 2 ; > F 5 S H 7 8 E Z / 6 > < 6 a 7 I ; K N U @ F D . < T : < I L * < J Z ' 8 J Z # A 0 R O A M 8 > H O M D D T G 8 M W X 8 U 7 1 K L \ 8 P M ) L C / W H ? D ' D Q J C / C H 3 L M 7 B L < 2 9 ? < Q F E K M * 2 B F : # 2 1 Z E \ # % - N N D 7 A A ? ^ 1 I 5 I S @ @ L ` T I [ D @ 6 7 K U 7 = 8 @ A J ? B < 1 > D L : C , = > E T J R J I @ G B J 8 8 4 > K 5 = : > D > @ 5 7 . $ 8 1 1 , * G - M 1 U = ? > A 3 = B H K K N 5 6 > Y X G < J < 3 @ C B L \ Q 7 ) 9 3 J 2 L > P D = 9 / L 3 = > I . 6 B ; * F C O E 0 @ B , 1 : Y : B 0 G 0 B L F $ C 5 - C ( F E @ : 8 J T J ' ; O h < i = . C I = : > F Y ? / ; 5 C > ; K F S U = 8 @ 9 J : N V * < * ! E Y ] 2 G = R Q + / 5 5 F @ J @ U D J F ; @ U = = R - A 5 Z C G 0 5 2 Q B 3 F Y G 7 2 1 A B I O ? S L I = > B > = ) \ ? N L O E P @ ? Z , F Q B L A 7 a 9 D C G M Z L O ? H > S Q A \ 7 A @ 9 = 8 ? ] > 6 J G F ^ + Q L A U X = > R A > > ; ? 6 A > J C C 8 @ P I M K J H $ 2 # O d S 9 h E H H K 9 6 C : 3 E & A > 3 C C B ) < H = Z E S W X @ 7 S E ? F > 6 B @ 9 % ; K d 7 0 ; 9 [ < Q S I O : E > E P L M A N ; P % M C B K F R Y 8 = @ < @ P 4 M J F C P Q V I 0 @ ! 3 : 3 ? J N N ] @ S > S J [ G - + ; 1 5 _ / K = A 9 H d - H R 8 ; > D 8 < Y D F 1 L > M = * X J + C ( 9 K 7 @ 2 ` 5 # 4 6 . 1 ; T > T Z M > : 4 1 0 > C D S M I S : 8 = 8 L G ? P 7 1 7 7 Y A D ? ? D B & @ E C M J K C V E 0 5 < 9 c N X H 5 : ? ' S F = I J I X 5 ? A U T E 4 ; Y M E 9 I A b Y H 5 N I 1 L ? & = + ? 4 F X R B 9 0 G J < F J = V M D M ? R K N O P 6 O A 1 D D > 8 @ 5 < < H ? F 9 K $ P 8 / E / V U C L P 8 : P C # 4 S O S K - C R I V H N T C K f b U J Z ] Z T J 3 E B M : B I C 3 0 _ Q N D P D D > 7 B N l > ; K 9 K J 0 E F M O N 9 [ K = / . 6 Q J O C = " . D G O R R P G A I D 5 5 @ % : D U G ; < [ 9 N ; 9 M M M ? @ M L = @ V P + 3 N > 5 ? V 6 B K ; N = / B > U C 1 9 P R a = > P H I W I F 0 ; G ? = . O G N B ^ G Q + : > P I A G P 3 E F 7 S Q 3 N M ] M J C T T Y 3 - 8 D C A = L F & M D 9 L N E 9 E ) N J N 5 J O N S > D O ` N V ` - > 7 C U O V ` 7 > A 6 a * L F ? @ < Q \ B J 2 a ^ < 4 C L G e X ? F B 2 K L B ^ F @ I H C # 6 R ? V ; ; U I P T Y K V O S R @ L G ? D C F O < D f C @ , ? R G C D K H < S @ 2 < B L = 8 A C J 7 L ; : - J H ? _ F 7 , R > K K 5 Q : B P G U D 2 ; = A M K @ Q I X Q 6 3 5 @ L A 4 < 6 K B B 1 5 A Z F M < N ^ A U I 7 ? d P < ? A E H - R ? P < D V E U D 8 : E B H Z F 5 8 D \ 9 0 9 ? > 7 A 8 7 B P C 7 1 : A M B O G M , 1 H H 8 ' 3 + * U 1 C F # % B > a D & E F > D > L 8 / 3 4 9 M ? C = 6 H L J K A ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8846b44595724a7999507ab5530aa5fcSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well K1340_F10_psbAKB_3730_POP7_BDTv3.mobSeqPop7z F` To*t8ukZmW@^kFkd>{K/0Lz 9#$ctQ
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KB 1.4.1.82018-08-01 12:26:59 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt d ASPt d B1Pt c B1Pt c BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G0 DATA
# G0 3 DATA # G0 { DATA # G0 L DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM j
P1RL j
P1WD j P2AM j W P2BA j j + P2RL j PBAS j j
! PBAS j j PCON j j PCON j j 3 PDMF PDMF PLOC j _ PLOC j
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% | SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt d ASPt d B1Pt c B1Pt c BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G0 DATA
# G0 3 DATA # G0 { DATA # G0 L DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab !x FVoc !g FWO_ GATC Feat . . !9 GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM j
P1RL j
P1WD j P2AM j W P2BA j j + P2RL j PBAS j j
! PBAS j j PCON j j PCON j j 3 PDMF PDMF PLOC j _ PLOC j
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% | SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH phDY big phQL c phTR phTR }0+wU]C.Bv|[Eh2!Ul628z>X}m ;gEF~ba+;x~Cm*qHE?OZC@weCS'J7)Ku3Be%{M*A#t5#r~^;?nU[PbI^6E]bZ&[VGgPH/#+-'M35FQ6n:.]X?K90plql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2WA /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DuYkqU*{Zq9Fy$C.bT8Poo=[/19~{yrA]#_WfOMt_`VWYhY8|kRYN4+HmLOKrPkWU-bek<=j+aQ!:NaKjZD(Z=c':Z^mDt4)TuTF}wKy3of)`|8&kS2BjrP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]SSK.N6jn6O1HnYDx8_b_l4P3LFRt( g>::#Qs60O'[W@-Ljs8G;_n9Nfcd]R039OQJM~V[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %> Ube1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&4&SG1T1M};<LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#