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` 8 @ H - 6 - + = I K 6 5 H ' # / # > 4 4 > R ; @ J 8 ] < 2 2 : > , 2 # . 7 ; - < 1 0 5 = A < 0 , = 3 1 6 C , / 3 " ; 0 5 $ 3 $ ' `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
; 6 5 $ ? 8 . 6 ) 1 7 % 4 . ) C 4 : , . . < 3 / 3 - * " ? V K I 2 7 - 7 9 ? 8 7 . . 3 ) 7 6 E 8 : : I ; ) 6 ; = ; > C H 3 H @ + = R $ ; 9 I S A / D B 9 > 1 0 > < : 0 ) O 2 2 - 5 - % A B + O 6 0 / ; : B 5 4 , . K O G O 7 3 B % ; I D # G : 8 M 4 I : > U F 4 H ; C H 5 : - I K > 3 1 = J 2 1 9 . . B N F A 7 " $ / < d 9 C Z Q E ? P ; A 9 ) 6 5 3 H E # 6 = & < P > 3 Z 9 K 6 8 C 6 2 0 = 4 ; 4 , / F ? > I R @ O J O H I 6 3 5 + = , U 4 % / - ? / , 8 " 8 ) A * B 9 ; M % ? 9 ( @ B 7 . 6 1 E D & ? 8 ( > 4 D * i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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7042bf815f254cd18e3dc756c9d5ed27Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:34 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ( LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
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RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
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BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
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PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
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RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
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