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!!!"""####$$$%%%%&&&''''(((())))****++++,,,,----...../////0000111112222233333444445555566666677777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bd22a565b88241668770e243da891fd6Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-01_10-38_01153130Seq1282912829_Steier_K1340_psbAjmadera96-Well K1340_D9_psbAKB_3730_POP7_BDTv3.mobSeqPop7z37>HVbhe]TQVct(:DE?723;HWdov}`G=CUjzyl\QNS]iqtqh[K<.$ }xx} *S9]~_-{S, ~-Ie<.CD5e'SE$R) 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"'%                                                                     #$                          %('!                      {wtrsttpg\RMSbx #-8BJOTY`jvpZG;;GZp7d V+Jy[ X<p^Xdy#_uDS`E5./34-I.~]IckcN-.wr(vP8,'$!#$7Pp :k)fp0DpS@2*$"+6DXyE,Seg^L.m_!|gYSV]flnmkheb`^^`cefeca___\VPMPZiw~uh]Y\dmrqj_QC5(#0>PhS( |/{tB.7U:<~mjruF^yl\LA>BHMOOSf8rR"bOKNPNF:-$!""$,?dX!dl/qyM3&"#&))'$ &.21.*&" !*.,$  ,l<NY3}c/$-22-&  %3?C?4$    EH&L #!      .?Rf|=aQLHGR'fQ bVD$Dy8eM;0,,,*"  #1=DD@:52001233349Gd&uMqUPXdjid`agovwqf[SOPTWZZZ[[YUNE=9<ERalpmdWJA<=@CB=3) #(++)(()(" D(d|j3P"1DQVRF3   )^NZq9 $#JJO@&'" &ecT[&  -o[t`$?&X h6  $,00/17CUi~ )Gb{=TblunWI@6#`D+i\VSL?/.ZoI+ 7i(uB+5] tF$ "&(&   !" #+00+#      &)(# '275, *6;8/%!#,6<;1!     #+-+% !#                   "   */,#  "&%! /NnrR2 4LanpfR:# "@_vv^?! .GZdaS?)   -BRXTG5#!)4>EHHFFIPW^bcba```_\WRPPUZ`cb\TJB>=?A@:0#                #    '**'"     #$     .I^f^J..=JTYWM=)   +8@@7(   ".660%               3L^f`N5  3L`kj\F, ";RcicR;"%=Sbf`O9"   ,<JRPF5"  =dlL2%#',*"     ()"                                                                                                                                                                                                                                                                                                                        !"                                                    [n@3AsAOA c KB.bcpKB_3730_POP7_BDTv3.mobtermTCGGGGTGCATCAAGACTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTTATAGACTAGTTTTTAAAAATAAAGGAGCAATAATCAACTTCTTCTTCTATCAAGAAGGTTGGTACTGCACCTTTATTTTTTTTATATAAAAATTTTGTTTGAGAATGTAAGTTTTTTTTACTTCACTATACGAAACAAAAAAAGTATAATAGGTATAAAATAAAGTAGTAGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAACTCGGGGTGCATCAAGACTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTTATAGACTAGTTTTTAAAAATAAAGGAGCAATAATCAACTTCTTCTTCTATCAAGAAGGTTGGTACTGCACCTTTATTTTTTTTATATAAAAATTTTGTTTGAGAATGTAAGTTTTTTTTACTTCACTATACGAAACAAAAAAAGTATAATAGGTATAAAATAAAGTAGTAGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAAC&:GS\jv !*3@Par{ '3:GQ_ku'3?LU`kv !,9CO[gt #/:GQ]jw+6BN[gs} #0;IT`mx #,8EQ]jt    * 4 @ L Y d o z   $ 1 < I T ` m y  " 1 < E R _ k w   + 9 D Q [ h s     ) 8&:GS\jv !*3@Par{ '3:GQ_ku'3?LU`kv !,9CO[gt #/:GQ]jw+6BN[gs} #0;IT`mx #,8EQ]jt    * 4 @ L Y d o z   $ 1 < I T ` m y  " 1 < E R _ k w   + 9 D Q [ h s     ) 8    $"3 /8#3'82.>;;;;;;>;.8>;6>;26.6>>6;;>;   "(8;88>>>>;;>;1>>;3;>>>>;    #".+8;;2+'++"1" "++$(+2..8(6- $"$"..0#(+(2.3;8.   $$""   "    ""! $!'+ ("'"""$     $"3 /8#3'82.>;;;;;;>;.8>;6>;26.6>>6;;>;   "(8;88>>>>;;>;1>>;3;>>>>;    #".+8;;2+'++"1" "++$(+2..8(6- $"$"..0#(+(2.3;8.   $$""   "    ""! $!'+ ("'"""$ #([Cab@B0[1@ *<S()I[A"LYKV`/c;4c(P!FP6I!]LHFB`># ZQ6I %  H`I.<:+T<EF#D ->"M;=> TR!cJ + @_MFG1 ;K,VTV$V6(,MH;b^" !BW4H:`D'KG*=O S7QN4RD _K;K8$$*2Z93;=Da%V+c!G[ CH2 52JKL$I4G $6)1_='@ID =@I-$\*]='8 E.KCEjrg ]):n0qCO' Yr_u$"Ol XR:7Z|$Fs h(@/Nytw@]C( "v 8Vv+=uCqosn1v3 Wmr L7KfJ5)6kwjtgrSLPO[V.>oM8`_/ KT[p7^^=ra)UNoAJ v.:/;ygAWKyEV}<uR9R(.Dq-OA94 !,M# &J   "&;H?,& "  .Z #ci 8, +@$%+1F7L.1 Bpppp0ppo*!,5S[?sZAa2ph[U_nIHq+l!U^ KZQj?CX$3$HD8//"s 1 1 q|L@k H t$ J^JUtEU[T)1Y?nuT1Hw-[E.b2_ J^ wNm|KmiO(@\\}^#YX~YAc> Z(<clIYjWuk$UfQ<w,L,75l0r7pS~=3n8tKH0"kCGAAAAATTCCTTTACAATATGCGATAGAGCGCTACATATACTTAAATATTTCACACATATTCTGGTCCGGATGGCCCCCTTTTAAGGGCCCACCTCCTCCTCAAATATCCTTGTTGCCCCCTATATAAGAGCACGACCAACCCCTGTCGACGGAAGCCCAGTGTATTACCGCACCATTAGTTTCTACACTTCTTCTCCTGAAGAGTCCTCCCCTCTCAGTCCGCACTTCAATCGCTGCCAGGTTCAATGGGGATTTAGCACCATCACGATACTGCAAAAAACCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-01 12:27:01 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS~BufT CTID{CTNMCTOwCTTL ȤCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA BDATA &DATA  DATA "EDATA "E2qDATA "Ex'DATA "EDCHTDSamDySN ȅDye#DyeNȐDyeNȕDyeNȚDyeNȟDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT &EVNT2EVNTEFWO_GATCGTyp!!dHCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~LIMS!!ȭLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISP1AM:P1RL: P1WD:*P2AM:P2BAP2RL:dPBASPBASPCON PCON |PDMFPDMFPLOC:BPLOC:PSZE`PTYPPXLBRGNmsRMXV1RMdN ]RMdV1RMdXfRPrNARPrV1RUND RUND RUND RUND RUNT  &RUNT  7RUNT   RUNT  1Rate XRunN""QS/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER sSVER,,Scal@Scan3TUBED9Tmpr<UserphARphCHphDYbigphQLcphTRphTRāThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS~BufT CTID{CTNMCTOwCTTL ȤCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA BDATA &DATA  DATA "EDATA "E2qDATA "Ex'DATA "EDCHTDSamDySN ȅDye#DyeNȐDyeNȕDyeNȚDyeNȟDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT &EVNT2EVNTEFTab(FVoc(FWO_GATCFeat..(GTyp!!dHCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~LIMS!!ȭLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISP1AM:P1RL: P1WD:*P2AM:P2BAP2RL:dPBASPBASPCON PCON |PDMFPDMFPLOC:BPLOC:PSZE`PTYPPXLBRGNmsRMXV1RMdN ]RMdV1RMdXfRPrNARPrV1RUND RUND RUND RUND RUNT  &RUNT  7RUNT   RUNT  1Rate XRunN""QS/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER sSVER,,Scal@Scan3TUBED9Tmpr<UserphARphCHphDYbigphQLcphTRphTRc:]FN}<,kSagv9'i?oX$"#e0}jm20,(1Opw?y-k$cexs^$5h2Nd,6Kl[Z9a!CHbT3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4OQSStT4VkJhGQBg[p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRb 2)FB[yEZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=+Iws?F!,"dIc5b-Y.pa6:!.tkj^4E2rW.X}=_;!;`sEY5_}4]'Z5P%?p MfaqQleA./q}.}:>JWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTc%{M*AqbEdG9}j~:HzpWI23Z;|*92UNM9hzOr:`M]AbK:f8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=KubQ2a50jj,4o-8N'nsv&'Y?sxWlZN.YHng=!AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtAUDTjj6B1PtB1PtBCTS~BufT CTID{CTNMCTOwCTTL ȤCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA BDATA &DATA  DATA "EDATA "E2qDATA "Ex'DATA "EDCHTDSamDySN ȅDye#DyeNȐDyeNȕDyeNȚDyeNȟDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT &EVNT2EVNTEFTab(FVoc(FWO_GATCFeat..(GTyp!!dHCFGCEHCFGHCFGHCFG==InScInVtLANEILAST~~LIMS!!ȭLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISP1AM:P1RL: P1WD:*P2AM:P2BAP2RL:dPBASPBASPCON PCON |PDMFPDMFPLOC:BPLOC:PSZE`PTYPPXLBRGNmsRMXV1RMdN ]RMdV1RMdXfRPrNARPrV1RUND RUND RUND RUND RUNT  &RUNT  7RUNT   RUNT  1Rate XRunN""QS/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER sSVER,,Scal@Scan3TUBED9Tmpr<UserphARphCHphDYbigphQLcphTRphTR