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q b f r s t p j n | b t W X S _ R U a g k T ` R l p l d W [ O R S M b z q \ n p g q p | ~ k v { a x | s v y s t u s z f f u k z d } } { ~ ~ t v l w i v b o _ X \ U e w Z [ [ k _ [ t V d M W f g w n ] [ \ d g c l j v ^ d ` M Y ^ _ d Z @ ` I Q b h S [ N } o X _ q c v l c Z a t [ w t b z \ { ` f ~ h R s b y z f a l d h r U R u p | l l b P J d l f h V f T G X g [ S a k ` e i X h e Z d q b p u q q x p x u n p \ x a Y b ^ w Q X ^ b R ^ Q P ^ V W _ Z \ < V L Z [ b p Y _ [ x g S f Q O Z h = 6 R Z X ] \ ` n t b ] o y x } x | l u q f c c i [ Q r J G \ [ ` W l B O e s a V | s a y | o P ~ s o ~ h ] _ f U R w w b k S q M ` R b a a r v e b i U b v h W V e b ] s } u i j x o { r f v f T b t Z W c n [ v ] V k b E o x i i | s d r r r z x z } u u z i s | } l z ~ z r k ] i v Z b h x i X { | v } _ i [ ^ c n t A s Y T _ k l ] r d s y v r b z u t } o p f I V J b S n z i H b \ D j P O U p b h n d h j P N b v W V [ V J Y Z U K p { r { r X R W n S Z U r _ V b O k V a a T m s r u y q u P O S W T k g W s m f n j e c y ] t \ r { f N u m o y t u | k x p s y z } g v o s r n x Q { q n l j { y k { g x ^ s j S ` n V l { h ] F I [ d a ^ e k @ ] L [ M u \ X U j ` Y R Z k t [ P P G W W P n [ V W M b q i W P a m u a ~ | i y w g w y u x | w | l q w ` _ r ` J b p [ T h Q X N o M K Z 9 W T Z Y q m y s u | l y Z R t | u u s h c [ k i p j d Y Y L U h S U V b U Y e ] L K d C b { b t p a ~ f q g p j g i h p g o i ^ ] e ^ d r K x h r l _ V j T R [ a u I ] ] M S b ] \ M J l q C N c j b X c ] A i j _ L o o i O m ? Z ^ a x J U c k c I \ F T Z p f U c Z \ i Y 6 P F K H X L e P X [ I N \ A @ ] n S ` b a A v ] b c B q L i ^ U a N S Q t J l c n U x w z M o v d b i V c b p q k _ r m o { r s } ~ | n z e n h m y k [ p | j v | o x Z ` t r k y r l ] W c c ^ X c E b t I Z U Y R O L Q P K _ r o r W j l m v z n D T ; Z 1 k U \ P D \ X I ^ \ Y b h Z Q h v s _ l k } | | t x \ | x [ t | V l } n u x r q v ^ s y s s w o w q ^ j a s x e X ` t ~ x ~ z h r P a O o f p H G F Y m x s o s T ] I m f a T _ m i v v p v { n { s y r ` i p ^ e ] O P p n q p c D o [ W k C M d w f v x r g t | d o z v s z y { i g b | v ~ o q e n o g l g q { y s { n t d c ~ m o h | k j d [ v u u m w Z [ p v z t r } x v m _ _ g f p p t w m Z O ` T T J W n a j p d Q W m b p h q i s w c Y y { U m p n x n y ~ x b q v u ` j z } | y t ` } h j n [ c k l _ a _ j f V b Y M e f O N \ c i ^ r w o ^ o X z s ^ k y r o j i p q r x n v g Z \ a i a f S Z D [ S [ [ T b b R M Q [ d ` ` W J d d t W ] Q A @ P S Q D X g ^ a N D H F g c ^ ] W e a Z M O O Y ^ \ ` f f M h \ c j X X j [ q s r ] e _ ^ W \ b ` p u Y h S r o h { v { ~ z z z } w b s t [ Y e T h b U S R [ ` n z _ u < g [ N J m o S ] k t m o ] b d U X v V c c u n ~ w k z Y T p = 9 Q b q M P Q U E S n X S k e Y W ] O O Z E m R Z e M S k [ a Y e W \ D O a Q P ` n g o Z P L M n K R O Z j a d V P b g x g S \ f m r c \ i } | u ] v { h x x e f a ~ x } | g ~ u U j m l z ` x s { } u _ g X y \ \ M o k V a a n ` K A a b k q ~ r ] p T j ] l y p ~ f f x ~ q ~ } g h u t h t j c ` m [ u j q | b v t x k h u l \ h l P K T ^ ] x Y d c x _ q m ^ ^ ] Z f N F X M Z S j T = W ^ m ] \ a ` [ o Q Y \ l i j i a h r e _ O v I Z Y N b E S ] d a P E Q [ ] e W O Y N q h Q I T W L L T 6 c Z U D F O K z R _ _ J I [ c Y h - ? R H E V X . + R I ; < D F ] Y O ; _ Z L h i h | i k g R N ; O f E 5 E F R J e U L f S 5 b ] V 8 F > ] _ V B W Y h h j k P c G r y w l p | | f m m X b Z I h e b D Y F 2 \ Q R S T ^ \ V R i g U 9 ^ T R b e g R ~ l g Y t t n v } r l [ y t n n m ^ ] M V T O D X J Z b W Q i T P = D T D R S 5 ` a b C Y K U = K G M n Q ^ Y a ` Q K S p P W j k Q s ~ i ^ W ^ m f X j f } b q W d g b X X D a V \ Q ] m L B X b E a ] H ] ` _ = B b ] Z M B N e ? P V Y i Q S N F X O R V X B V c h K Y P > X K O ] w ` R W f E V C P K \ G I U Y Y i P P Q P W Z G j f f @ [ Q I W [ T 4 W M k b 4 K T k _ ] a : i U Y M [ F U e Q M F U I 3 ^ q H M C ? J T N P T U W h T V Z L M L ] O H I V = G O ? H 5 B H N W F J C Y X T _ J d R B M @ \ J Z P ] = J F C > X X 8 S K " V O P F C T Z N J R W ` a J I > A U W g d L U O N H % W 9 E ` N L H b P S K S K 3 5 ' E ? 8 L I U 7 > G T O [ O [ P A K W N E f Y G I W T O E N O \ L b V D P \ b h H U R e H Q E R C M M G : C 9 / R O ] D Q I @ H I Z N s W Q D B M I ' M N e ] ] = 9 Y S D J 9 N o 8 B Q S G 7 D q G S H U W = > O N ^ X V X E ^ U Y / G B _ i _ K = W X ) S ` G S @ \ T ^ G P B R _ = N c Y X < J Y b z g [ C @ [ Q N 4 G > J C d f U Y 7 H I ^ M S o R E Q : \ \ [ n \ ^ R J H J 4 R ^ > E W B ] H M E X P M 4 J D ? ? & Z f N N v _ a Z V Z W B ] [ N > R K a Y ? ] ; b m b X g K E , _ < T I S I L 0 @ Z < A L g b P K S I Z T T F W o l B B V E ] H \ f G ` a D P N @ N > R J K X c H = d D X 7 \ R I < K K 9 D O B K J S ^ \ B E G H R Q F E U L Z A < W @ Q [ Q J D \ G 9 C K K Q M D E 4 \ : H X U < T 7 r [ H a D @ = 4 > N D ? J M \ 4 X N / M I 4 @ A J S N T G B 8 L : F E J L 8 / G Q @ r C R ] ^ J 6 4 > = S I ] P 3 G L L V ? D = 2 K ? > S ? 6 @ / 6 > T U B I J U E M _ P _ g J R O D ? K B M G T B V L K G W ; H N P O K ? : @ a M S H Q : D / > J [ J O B 4 J H J L G _ - L I V k Q E I : W L R Z 8 O S 8 E ? < Q H . 5 5 > G O C 5 > J I O H A E , < V : L 9 X [ < 2 4 4 B K 3 R 9 ? C S 0 F 3 V a T G B 9 e < ! I @ U 1 B T O Z Y Q Q @ 3 U M Q T D Z R ; D W O D = 6 C O H ? ? F = F Z S U K P E > N 6 O O C C O W K R ? B U J : F P K R 7 A K B D F R < I S 0 9 E / K V W > 5 E ; E > , A U < Q G ` L ; . D 2 5 ; J A . + G A > E Q 8 9 6 H : Z V W I > @ = K M M O H U F B 7 R D O F C d F ] V ; C B G N L O [ I T G F K 7 C O = K E ? 8 : A G E > H O E 8 D < U W X R F K Q O J = I H H I G X Y G > Q [ C B L ? 8 F a ] i O K D X 2 > @ ? G A ? J U ` J S D 1 L T I F ; L a ` B G M i ? U L j @ > C X O 9 E S L i F D N P 6 > = I 7 \ ] ? E 5 N = F T 9 < D 5 ? A > ? Y @ F / 9 E : ? B @ V > K K 6 1 R V 3 U W S j L : . K Y D S C Y - F O ` 9 O L F : W X B G = P = a S D F Z N Y Y K G > < K I < N X N 6 < / O 9 [ R ` 7 U 2 \ F < 8 A D E = R c ? ? I D W U A G N P T E N ! > V I 1 T G H B O [ a A < M ; : 9 @ > O U U ] G Q W T = J U / 9 G E d V E T @ K P L Q W ; G Q ] D S N Q J V ` Q D A D S C T N P O 5 W R 8 / L B C 0 F : N 7 i E , < H K C 7 8 M ? F S T G 4 A H ] Y c d 3 = @ F F A ^ I B K 2 ` P V V P _ S Q P ; @ D J O 9 @ B E ^ ? K Q I A Y = . J W I ; F P O ) 6 P N H 3 I 0 c 8 P M ; C = - I F D T E D M @ G O = 8 Y F O B @ K \ Q \ ] B Y ; Q A X M G F X P J S F Z R J I X 7 Z Q L a K [ , K Z Q F Z V T 3 < Z W E Y H S Q K D F K C Q ] I E _ F H h N W N B : J N U 2 7 . \ O N 6 O B A X \ a B C ; + N \ < K O G R = R H 3 O P D Y B E O G M A - ! E ` 9 ) 2 ` S e 7 J C K ^ \ b D 8 J W ; M 8 I J M Z F T I ` O C C J R S t K F V W B ? M J P a G @ K ? _ = 7 Z ? 2 E G 4 L 4 : D L V R J \ A ; ? O G H ? ( R [ K ] M Z Z Q P \ H S D P M K L K K I D N C N X B f Z S [ N ` E J W Q X X K K Q ; O 5 P M B B M : I : : N Z Z N E ; C < H I F I O N J J V O C V G M < F ? 7 < G D T J V 8 < B V E K B C E S K I T [ e @ ; Q U N ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 61e85584b06b4138bfe999d0b7236cc4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
K1340_D7_psbAKB_3730_POP7_BDTv3.mobSeqPop7z - + ) ' ' + 5 C S b m t r j ` W S U [ ^ Y H 0
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D U _ SI V+ \p MC 6 v?t6 g }j )q R ^ AGTAATGGTGAAGGGGGCAACAGGGAGGGAGACACATATATAATATTGGTTACACCTCCGTCACTCTGTGGTAGGGCCCCCCATGGAGGGGGTGCGCGGTAAAACAACCGGGGACGGGGGCGCCGGGTGGTGGTGGTATGTAGCCCGGACGGGTGAGCGGGCGCCAAGAGGATAAATGGGGGAGCTAGAGTCAGGCCTCTTACATGGCGCGGGTGGGGGCGGGGAGGTTGGGCGGCGGGAGGGCGGGGTACGGGGCGCGCACCTACGAGGCAGGAACCGGGGGAGGAGGAGGGAGCGGGAAAAAGCAGCAGATAGGGCGGGCGCGCGGATGAGGGGGGGGGTGTGGGAGGGTCTAGACATGGGCAAGAGGCGTCGAGGCGCGCCGTGGGAATGTGTCCCTGAGGTGCGCGAGGTGGTATGTACGGGTTATGGGGGGGGGACGGAGTAACTTCAGCGGTGGGTAGATCCAGGGGTGGATATACCATAAGGCGGTATAGGGACTCTGGGGCACAGTGGTGTATGTCGGGATGGGTAGTATAGATATAAGAGATGCGGGGCTTTCGAGCGCTCAGCAAGCGCATATGCGCTGAGATGCAGTAGAATGTAGGAATGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:49 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt p ASPt p B1Pt o B1Pt o BCTS 2 BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " E DATA
" E 2c DATA " E x DATA " E DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ 1( LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM f P1RL f P1WD f ^ P2AM f ) P2BA f f . P2RL f %* PBAS f f PBAS f f PCON f f ` PCON f f PDMF PDMF z PLOC f
. PLOC f b PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
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& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC A* SPAC s SPAC A* SVER 4.0 SVER 2 SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR 4* This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt p ASPt p B1Pt o B1Pt o BCTS 2 BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " E DATA
" E 2c DATA " E x DATA " E DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab A FVoc @ FWO_ GATC Feat / / @ GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ 1( LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM f P1RL f P1WD f ^ P2AM f ) P2BA f f . P2RL f %* PBAS f f PBAS f f PCON f f ` PCON f f PDMF PDMF z PLOC f
. PLOC f b PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
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& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
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