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~ } z r p v q o n | f q j o k q Y i a r X g W O ] w W ^ [ O M s n g Z j h m c g { m r p { \ x r m t { _ [ p y { y { i | z ^ h d X q t { { o i a j p z y p p Q h r x m w g W U f v h x h m j x f ^ \ W t b i [ R _ \ X e g l a g a y _ { L s t m b g l v ^ m i o p r v [ W d i S ; ^ ] J = m r P ` z a m l b y k e Y \ H \ \ S ? P Z H M Q P M J d Z m X 7 D c ^ U \ c c i U K P B ? D l Y Y _ Z ? [ U \ V S Z I K g ^ P V K W \ J Z [ V @ O X K [ I _ ? e U L f T > C P Q X a c Z A E a W U T \ r g \ e O [ _ ` Z _ X _ _ A U W R [ H X N G b V F J ^ r M X < \ R V S = h U h c V I V Z [ E G U b O I g ] L f F N Z E K S M c e E J B P J W Q ` V V k ` ] o L L 7 ` O ^ V _ Z c _ Y g H B @ X U E g S l [ V P 9 P H e Z f O N @ \ D Z A R ^ Z P b B j X \ 6 Y h C K O [ [ C V R C Z O ? H Z f [ I S A h V W R G [ T L W U Z S V N 2 V Y A V ? ] K ` ] R = M Y J B Y ; I W = R _ Q L S F a = T V Z V ` [ X g _ ^ I I ` O N Y I _ J U Z \ d 9 = \ H ^ U ` T R [ W F ^ Y @ H ` Y k e m c S ` A Q D K W 6 ? Z = < : f W @ a g b c d f a g S c V J R U Y J | d R R a Z c u \ a Y L e E V \ U R J > N d U e B 7 L e t < U > Z W a R j S \ V ] U g @ T M < C Q d _ C X ' E \ T f R ^ P b @ 8 > N W D D O ? Y ^ U O M { Y T O b i S V T F a s k H Y Q Z Q s V K > U Z Y N Q _ d \ O g h N U X X a T G W ` ` T \ ` U l f [ i S Y ; J G K @ D N @ ^ B @ Q l b i T W M P K f U a [ d P S P = P m S P R V l U T T _ H V X M L H I i V n _ Q Q T \ G h V l K \ S c M l ? T R [ X K a K K U h Z [ W F c \ U E Q j y h ^ e e ` ^ @ S T \ [ ^ N E J Q _ Y Y L O ` b @ [ Y g s X 6 N L Z P Y I [ f O d q V d _ o O [ J A R L L V U Y G D E Z N ^ Z G G [ ] Q T M I b Z ] K P @ \ e W c Q i d Z R E e I Z d ^ h p ] e N x G @ 1 M Q b O j N W V Z W a c I : ] k c \ a o N d ` N L f F ^ W T V J ^ ] S ] N Z I c S J i g T X B \ @ d H < Z K \ = ? X f j Y T S S c b 9 \ T c O m ] a ] N _ d W a N R A R Z S Y P M K B W S T K K O L S ; W Q G = F M S E ^ _ K k T \ B ] Q [ F W J O L [ q R e Z [ B ` L a [ Q P X O ] K N H P < ` P [ ^ W B G E [ Z Q M c ` | g c O k [ A P @ R I @ 4 T U L V I ] \ ] ] D I f c \ u R E V R J J A O T [ P l q ` [ E f O S b Z @ I W j ` M K R w J c D N S @ X ? X ` f c E Z O G 8 G W h 7 C V ] I W Q P H R Q a o S V ^ R \ H X _ R T F I 1 _ : 7 Q d J ; P X Z a E P T I N Z @ J Q b H E Y N l g H L P G ] V Q ] D T Q ] P D N W O h I F U K I U T h S V 9 W p d A K K M N m I N W c H ^ K L O R e W N S M V Q U L \ : b M B Z T Q A Y K e X c G W ] Q _ a \ P < I 5 ? F i L T ; G \ O B P H < G ^ k K S b d ] b U X d J W S j J q Q i H Z A L R y ] J Y = ` [ M ` S c b S g O ] \ Q U T R b S f b e d J j Z Q A S K A [ ] Y R S : J U X f X V G C ? K G T f ? S X L ] ^ T a I R < N K S F _ Y 6 E ` H I D Y M Y ` [ M O < O k D @ I J W ` S y ] Q O E O L _ H b A K H E Z L b b e @ R ] ^ J U O E 2 [ L [ ` \ R f J D S H c X W R a R P E X 9 0 B R Y S P H ? J e W X ] Z _ V I L o L d P Q K P 8 4 ^ K X I P N A J I g G \ j D Y O ^ Q = e [ S P g [ d [ a f [ c E ? O N 5 b a b X i [ R r F r P O N E ; R V X U N M c Y P Q ^ 3 f S J Z L U 2 S S N d K > ? @ F L [ T h \ b U W \ Z P E V J Q T J 1 G X Y Q U ; W K M J L J C 5 [ G X U D N H f T L E V H d L Y O ^ I I D X A I R G [ T 7 c J U W O } K ` ? \ [ ] ^ L W g G ] E M \ X C @ 8 J N X [ b V O : S _ Q ? V M d L G [ X > Z C H 7 F L G G X N W S E R W D O G r N I W S M b _ @ T K D E M H T [ ; J U Q @ E U R K Q d W ] O L 9 F M U ] 4 [ \ M g Z l M N F Y [ F = N g O L U T L < P Z V I [ V R G T R Q S 1 C 8 [ ] T Q A O ? T @ T W G 6 W P B L F U , Y X P J = F B _ _ M ? R G R : B \ : J b N K O I Z < j Q > H P C ? S @ G ? K H 9 < D @ < W K Q = L T _ H 7 M P H Z E [ ^ Z g ^ J D C R O W O F ; ] O O ? : c P F P I J ; H V M P P q B V k X P S J [ X O D H G U 5 F n B h V H F N G ^ a L P F C > D U M X T T r U W > R V V b B E e k ` K N M P V = 6 C O j P A A 7 D H F 5 J G ; 6 7 N R X E I S V : 4 C = A B K L E [ \ O P 6 P N ] 7 b [ X ^ e R Q 2 A S R W E p L N E Z Q ` M J ` a O a J 5 Z J l [ / e ] 7 X k > A D ] Q W [ D G A Z W a 6 V N V c 1 > _ h O 3 8 R S I H ' Y X E F N X U G P F S _ C 6 Q k Y T Z V B ] R A 6 G k I ` Z ] ^ O E ] J D \ C S Y S N U Y Z R J \ P h d ] @ ] O V H h ? d H N - @ c O d K ^ b H N P W F I X O B g Y V N ` K A O ] W T L N X H V Z L O ; [ Q Y A < F b F = 1 > I a H a B U ^ T E X U L L R J P J M i X E e Q G \ B H R ^ S H D Y S Q ] Z A R N [ = M M X ` R A Q C T T @ Q X 0 C I ? F I d g . Q 2 S ^ H V W N C Y Y G V U L T S F c H ? L X ; [ M [ l @ D N E ? T M [ A U K K H F D 5 I G L R . D 0 _ A ? L T S R L I > U Q O > < a M R I E D W C O W a = O f S E J N g S O W N ^ X 1 ^ I L Z 3 5 6 = Q \ [ D = Q = G / L T S B ] D P M L + J ? E V e m L c H _ N L > A N N K F R > * 9 I H K 9 T R S + J h C t ` \ K L W 6 L K V M R W T F @ [ 1 < ] ] G ? j ] Q Y V I J N F 0 O Y J Y I S W O J : H N Y O V K M : H c M Z Y W ? Q I J U U A J Q p < R \ P W [ R G X U X U S ` W E @ O T \ O [ = a C B E H > < 9 g W T c K @ F A \ W ' F X W a K [ @ L Z P N Y t ] ^ P _ S B 9 Z [ B 3 \ K 8 ] G r K 3 F Y i ] _ T [ _ Y ? . Y ] ^ A W T X c X [ E V H _ Q F _ ; ? N N F Y ] U > = N M S \ a ^ S Q I 8 N A D X D O W p _ @ R Y ? J P 3 T W K a E < : W Q K Y H L R = 0 V I Q \ A \ B K E F D b W = V G J c ` a [ B b E L 9 Y L H L I 9 N O M B Y T I ` ^ J ; N @ Z ; S N @ S Q A ; W ^ T M W F I R ` O a @ ? J b N R Q O c J P 8 Q S G G I G E [ Z 8 I J U U E C D T V N Q b 8 U R K Z U a A 7 > d c E O ` g 6 K P Q H G C \ X e M I J L Z f H [ A 9 [ ` K W \ D K H G B ? R ] d W C g M V I p N R Y Y S g S Z C G B p d [ N I X D : \ H N B T M H < ` G D ? U E L W < Z L R L O ' 5 H : Y [ : R C > e 5 P C R U f S M L 3 A K T W E R M Q T A I L V B F L W [ P Q J T R X N f C A X ? S V P X Q ] d R Z J M ? D M A r K @ I T ? I T P K R K \ M M S J L T D ? y O ; $ c 8 O L < K 5 M U 8 L R L D [ I A F A V G d K K C B 2 E 4 ` A 0 X D V A Q P 4 O K I J K e D Y R K ? Q S D I K D D _ e ] ` D O f d J D B H Q ? M R i U L N P b S L 4 S I O H Z N K j N F @ O A , T A Z f f e ` I F S i c 2 V J I + g X N P ? W M I = @ M 2 Q R r J L E X Q K _ P 5 ^ A D C P \ Z W K @ F [ L ] A N @ E B C R R T > B ` a C F R [ C N \ Q a @ U K J A X C ^ B P j , W K 5 Q L 4 D 7 M M X O N S \ Y C D S N b J S e ] U Z Z K Z > E : Q D a b O ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f43a350159aa4a90b415ce45745c9484Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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7 Q Q N o * F g J & 8 ) v F 6 Z M v . K B B + F f " ? ) P 4 K e h > 8 p q ` I % p _ q X uf A\ V NGCGGTTTATACCCCGCAGAGAGAGATGCGCTACATATACCTAAATATGATACCGTTGTCATAATGTAGAAGTCCCCCTTTAAGGGGCGAATAACTATTTACTTCTGCATATTGCTCCCTTAAGGTTATAGCTCTTCAAAGAAAATAAAGAGCAATACGTGAACTTAGAACCTGGACCGGTAGAAATAGTACGCTAAAAGAAACGCCTAAACTCTGGGCGGACAGGAATATAAGGGTAAGGTGCTTAACCTTTCCATGAAATGAGCCAAACTGAACTCTAAGTTAAATTTCTTGTTGAGATATTGTTAACCGTAGGGCTCAGAAATAACTAAGCGGCCTTAGCCTCCCCCCTAGTTCAAAGGCTCTCCGACAAGGCCCCGGTCGAGGGCAGTTTTCTCCTATTCACCCTGGACATGGTTCATTCTATTCGTTATTAGAGTACAATCTCATATATCTTTGTGAAGAACTAATCAGCATAGCGTTCATGCATCAAAAGGGAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:36 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ' BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " E DATA
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SMLt SMPL SPAC A SPAC SPAC A SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE D6 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR 쁅 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ' BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " E DATA
" E 1 DATA " E v DATA " E DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab 6Q FVoc 6@ FWO_ GATC Feat / / 6 GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE * LAST ~ ~ &m LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM P2BA $x P2RL PBAS 3 PBAS > PCON PCON PDMF PDMF " PLOC PLOC ( PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC A SPAC SPAC A SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE D6 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR qCfkNR(Bj'~;Hk@*w&RA#] lM6[/:){hV(wX>?7u%#F7Np1mj;RJJ4R,9X PMF9zH'zAuR)x|ec{PG6x|W"OKOfx&i1PBEe>2flV:2/dmm YJX?/WZ#}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMi?_ A([Ne(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mq;;9VgO(_y"`k:!.tkSY4E2 (#>+6QjHzpT:ygBGqg_f#V&S$~=}F)h>;-T)RJTZmU)P? sIe]RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/Ygn PCON PCON PDMF PDMF " PLOC PLOC ( PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC A SPAC SPAC A SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE D6 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR