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o w q | } t { _ g { Z n s v ~ o a Y j o X ] O \ S N Y F [ G U U ] H @ U W R Z ` I X P H L K R 3 H W M S U ] M ] K F S 7 ? B W M > [ < N G 6 J L [ ; c J H 1 T / J [ P O A M > . T 8 T 0 ; 7 H Q R G 0 3 3 = M < D 5 K 5 J G C P Q = Q I O R K ; [ = A C S P F ) : C E ; E M 9 C E 0 / < P , E > 6 ; = G 9 9 0 9 ^ M Q 7 ? : Q F , 8 F > / H 6 G , D F G ; M B X 9 J F H _ ? O W > 7 A C K 2 = F 6 > C 5 b 5 : < 2 V 8 > 4 K = > N R Q > 4 5 6 N 7 Q > 9 C T C H 7 H 5 @ Q N 0 E > T I @ > ; . : 7 * D = 5 J T J 5 B 0 + > R @ 0 ; 9 0 : J O F N 3 L > " & S W M 5 ( C J 2 / J I H . 9 C I , > N A - 4 7 2 3 = C 1 T J D C & + 4 B P U % E / + C H C 6 : . I # X 8 0 K F B U : Q 8 ' 4 J I > * 7 B : 3 J D G : 1 > K ? G = 4 A D : P < K E , 3 4 = ` L W ; - ; U F B 4 S > K R > J R Q S I N F < I / O O < L : R V R V I G F ( > @ < F > L b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` C > E A @ I R @ = : 8 V B F < ) E B = F B 9 7 B G = [ H 6 R G @ I I @ R 6 L 5 < 6 E B / 9 K ( 8 D 1 4 C T 3 A > 9 M 4 = E - % N ' / 8 D 8 ? ] < L B ? @ G G = C A G @ F C 8 1 2 ? > : 6 3 6 A J > 8 3 8 ; A . ? % = * ; D P P H A , / L N + - B > & < c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y 8 < G 3 7 ' 6 > - + @ 1 + 3 5 0 > J F 2 2 3 . E G 2 : I R 6 3 F - D ! = C 6 , + = D : : 4 % L + ) > 3 + > - ? I / # ( U 6 7 B 4 ; 6 , 7 8 = ? N 8 S 9 6 A 8 > ( ) 3 > A / > B ; ; L : 0 ) 4 5 5 A C . < J 8 * < ? S < F 0 : 4 C ) 0 % ( * 8 I D 9 . 8 ; $ 3 ; = F 4 > ( K . . * 0 9 7 < + = 4 < > . I O 8 , & & 5 E / H W M F J E B ) 0 1 / B 3 L 3 0 & / ; L C ' & @ O F @ ) C B C 9 7 . - A , C 8 @ 4 * I R D H 7 C ' ? ? > M @ 3 ' = ? D : 9 1 : 5 4 + / - 8 L G 4 5 H 9 9 D < 6 6 ) + . : : B 0 + - > H A 3 4 : 6 A 5 0 H , 5 2 + - A 6 : > 4 3 ? I & 8 3 J 4 7 E ? * ? 9 / N L 3 C ; = R A / / C 8 1 : 3 0 & 1 ? 3 I P 6 = M ) 7 * ; 7 : 9 9 > ) 7 - + C : = % % 9 - 6 D 2 M F 9 = 7 8 < ) 5 . ? K 3 1 8 6 $ P H ; + ' 8 A * 7 6 6 3 ; ; ( P = B 8 ; C 7 : ; @ 3 J E H + 4 5 3 ; E 3 - H 7 P @ . 7 = 1 - N C ; : 1 6 H H H = A E A G G @ = ? , 5 < , * 0 7 @ X > ? ' = = 6 1 + H 0 H P ' @ ? # J 8 K 2 5 # 2 M M C 1 5 [ 4 9 ; @ + : 2 6 : 5 B 8 3 E G E 8 P / 5 2 $ + 7 . I - 3 B 8 X 5 3 B * Q ; R C / . C < 2 I > ; I 5 B R 5 1 , H E R J C 9 4 = 5 8 @ 7 0 4 M % F F ? 6 A 3 9 - / < K L G R 4 + M E F 1 ; L 7 : < ? G 7 = ? : < . @ J " ) E 9 0 2 > J A 0 , 9 C C 2 ? 6 F 7 D 6 T 1 ; L E , Q , * 1 * + : : 5 3 @ / C : ; 5 6 T D ? 3 0 > 2 9 C 2 1 " % B ? ' 6 A - 9 O 7 A 4 9 > ' ? C < ; $ 6 2 C C K B = = M D 7 M - # D & F = 8 > # 6 E 8 6 + 6 A P C E / A * / * + 9 6 G : . E A 9 1 H @ D 3 0 / A B @ G K P . I ; - : / O / 9 4 D D E 7 , F 2 % ; * F 4 * B - O N : 1 4 4 0 ; < B ? , 7 : E L = G C 7 . 8 A B B 3 2 < J C D E 5 E 4 E ; 4 Q + , Q 3 9 4 A + 3 A L 4 . 1 : 4 : H 1 : = + - 7 / 1 ; . ' A G H * 8 . 0
6 7 0 : E : 8 ; > < 3 D @ 1 B F D 9 ( 0 ( ) > ? Q M ( > 4 ( * 3 > 6 9 7 4 6 + 5 G B < 9 + 3 A A G N > b ( 7 4 C ' 3 . G 7 % 5 9 / 6 8 7 T D 2 ; > $ * @ 0 ! 7 D ? 0 H @ 7 F / = B 3 8 H # A * . 5 @ C + K 7 6 T 5 / * 7 ? . 4 8 F 3 ? + % 2 1 8 5 < 3 > C H E < ; 8 2 1 4 D ; 8 - . 5 G 7 8 8 F * ? 8 B O M 7 , ' D F C 5 / 5 D C + G G A G I ; < 3 3 D I 7 H 5 5 + : L < 9 6 < > ? C @ 0 & > 7 ; 9 D 3 : M R = 7 5 4 4 N . C : M @ 6 I $ 0 % @ % ? ? 8 ; M ! 1 0 O ) , ( 9 3 1 2 2 2 . D C 3 ' , 9 E @ % B < D 8 2 5 8 ( * , 1 C 2 = ; G 0 G B 9 0 3 ) [ 0 C . A A 6 * % 5 P B 9 J ( / ) : I / ( R > 7 E 8 M 3 2 , : $ R B D 3 S 6 N ; D ( ? ' 8 T ( A < d B G 1 9 4 \ L 9 - 5 D B 7 G ( L N F 2 0 2 A K 4 7 I 0 0 6 F N M , K 9 % 1 C W E ) ; C ! L : 4 B W , # = P 4 , - - / : E * & [ ? - , 9 , 0 3 1 8 E 4 J < 2 2 * 5 Q 8 U L D ( 7 7 . 3 E 2 / ! 2 8 > 5 > I : M A ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b8b63c8d44d94b6bb27fd2a5aa7b37caSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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+ G 6 d x W 1 : 5 b Q # 7 < } : ? V P p } 5 5 D 3 d M B 4 K ) a F w ? > 3 0 k w 6 X Z + b ? l d 2 o E > . + K - S ~ L ~ $ y N ) K v , C T v 7 ^ " $ $ $ N 8 6 < N _ 2 = c & & Z / ~ @ 8 ` G M * j q 1 g $ _ ^ R+ a t k 7 B 8 ~r} BCAATTATCTTTGCACATATGCGATAGACCGCTACATATACCTAAATAGGACACCCCTGGCCTAAAATAGTGTACCCCTACAAAACCTATTTTGGTTTTTTTCCTTCCAATAAAAAACCGAACCGTAGCCCGCCCCAGAGAATAACAGGAGGTAAGCGTTTGCTCCTCATGTAGTGCCACAACCCCGGAACAATACACACAGACGATAGCCTTATCCTCTTGACGATTGGCCTTCCTAACAACCTAATTCAAAGGGGGATTGTGAACAATACCTCTATGGCCAAGAGCGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:23 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS * BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA "g D DATA
"g D 1 DATA "g D vW DATA "g D % DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM ! B x P1RL ! B 6 P1WD ! B
P2AM ! B > P2BA ! ! P2RL ! B PBAS ! ! O PBAS ! ! . PCON ! ! PCON ! ! PDMF PDMF PLOC ! B PLOC ! B p PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE D3 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS * BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA "g D DATA
"g D 1 DATA "g D vW DATA "g D % DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab % FVoc %t FWO_ GATC Feat / / %E GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM ! B x P1RL ! B 6 P1WD ! B
P2AM ! B > P2BA ! ! P2RL ! B PBAS ! ! O PBAS ! ! . PCON ! ! PCON ! ! PDMF PDMF PLOC ! B PLOC ! B p PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE D3 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR ,qJ'8f7j}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"EC+CCWVT6U3P.4V\1OHfNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\cMbYJ}whAbH6X12g[jWcgiQF4Np3`4 x'gJx%*{#ODYnWZjdO{7vMjL3ro{`N%7`h?I8rBArIUwch[}k@_a'>yj6m}P,PHq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,61h67{=Y`gI)_|_n?NhN{CwN1:^M*92[sghT:Cr:}u=(tc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`"/OCf7uihukWy4=?6vtfQgsk>H~pL"A~oTq}}89uV|Qw(xvU+SI.N{,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS P2BA ! ! P2RL ! B PBAS ! ! O PBAS ! ! . PCON ! ! PCON ! ! PDMF PDMF PLOC ! B PLOC ! B p PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE D3 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR