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J < > 4 N P N Q ' : : S 9 B A ) 8 C U 8 E : : X 6 2 = 8 > C 8 G H @ P ) 1 3 9 ( 2 1 ' G B 2 C C F T I O I $ J V b < K C R = < < J G @ 2 ( ) F A ; L < A < 7 4 Q N > 9 @ J B 3 ; H 7 ; : A * C . G = 3 O C T 3 ( F I D G S 5 H ? 9 C E I 4 P @ 8 F = ; B > - F E ? ; % A E A = > < M H 8 & F C 9 2 5 9 A M X H C ( . O D F < L G > ; 8 ? S 3 > 9 P 2 = 7 H < G , L * > E @ - G Q K D 5 ) C A 6 ? > E : B < C ? M 5 + G X 5 7 9 0 ? < J I 1 Z L J 5 5 ) < H = I 6 * 7 M [ P ' 6 N < I 7 K + 4 @ : J 2 , 3 A < I I = @ = 4 I = @ V O # P < F R C D 9 L 4 P 9 ` 9 6 > / < D J X O W = I R F ? G 1 : F K N L G < @ D A 1 1 3 < ? 6 = @ C < @ D S = C 3 L ) E # M " 6 . 7 6 1 C B L D N 8 E ; < 5 = U - F N @ N U Y 1 = ? - ? A K H E - 8 > : : / 2 . = ; < / 3 B > Q K < I 7 ? C $ = B 7 9 R A C 9 ( " 8 6 = & 1 ; > T @ : - ; @ $ 1 / 9 ? 0 E 4 K 4 @ B A > 7 < P < & = D L 9 4 W 9 S A E @ D & P I M c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a 2 2 E 8 / ? A D ? I F J ) ; P 5 J 6 @ F . B 9 M C + M 9 - ; - E B : 3 7 - P ; b 3 ? 2 @ J < C 0 0 J A B = E C G 1 7 K % : ? @ I : : 7 P O E 3 5 1 4 5 7 F 5 2 K 8 M C 8 I : S > : E ? 0 I 1 1 ; : ? ; J 5 < U < D 0 G ? L I : L S A H B : G # L ? 0 ? / W 4 @ F 1 B 3 ) 0 ? A ? 3 ; V @ = E 0 5 3 P S 9 Y E : O E U : * E @ + ; > L < Z I G - = . H Q F , B < O ? , & L i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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 04e8fdd81f2848a386ff6e8e6dd83c93Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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[ 02 4 < A E^ ( ] 5 5 = Jf x , }Z EQ < A 5 + Y 6 0` . Z0d 3 ;E E (> 9 u <w %"*q S0 l 8 #45 d 0 1 S % E P 1 " Z W o K 7 LI @ Z +@ O^ 6l P^ _g n><* #0 u - 9] x x 6I 0 b} B@ [0 2o p l v $ wA R L = AAGTAATGTTTTTGAAAAATCAAAATAAGCGAATAGCGCTACCTACACTTATATATTTTAGAACTCTATTAAATAGAAAACCCCCTTTTCAGGTTCGAATTATTCTTCCCCTCCTGATATAACTCCTATAATAGGGTTAAAGACAAGAAAACGAAGACCAAATGCGGAGCTTGTTGGAAGGCAAAAACAGAGACACAGAAACATTCGCCACAGGCGGACCAAGTTTATCTCCACAAGTTTAAATAAATACCAAATTACAATTGAACACTCAAGCCTGAATAAATGCGGGCAGATCCCCCCTTCGTCCTTCTCAAAATGGGGACCACAATGTATAGGGAAGGAAATCACTCGGGCTTTAATCTAAAAAGAGAACATAAGAAGGGAGCATGGCCCCCCCCCTGAAGGTCAGGAGCGTGCTTATGGCTCCTTTTTCATCTAAACCCCCAACAGTCAAGCCTGGGATCTTGTCCAGTTGTAGTTAGACTGTCAATATCACCTAGTTTTGAGCATAGTTATGGCCATTACTATTTCGACTACCAAACACGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:37 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt R ASPt R B1Pt Q B1Pt Q BCTS / BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # F" DATA
# F" 2 DATA # F" x DATA # F" ! DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE + LAST ~ ~ . LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS K P1AM # F P1RL # F P1WD # F " P2AM # F ' P2BA # # + P2RL # F #h PBAS # # PBAS # # ~ PCON # # s PCON # # P PDMF PDMF b PLOC # F
PLOC # F PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% C SCAN 3 SMED
SMLt SMPL SPAC AYO SPAC [ SPAC AYO SVER 4.0 SVER / SVER , , Scal @ Scan 3 TUBE C5 Tmpr < User phAR phCH y phDY big phQL c phTR phTR & This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt R ASPt R B1Pt Q B1Pt Q BCTS / BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # F" DATA
# F" 2 DATA # F" x DATA # F" ! DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab = FVoc = FWO_ GATC Feat / / = GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE + LAST ~ ~ . LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS K P1AM # F P1RL # F P1WD # F " P2AM # F ' P2BA # # + P2RL # F #h PBAS # # PBAS # # ~ PCON # # s PCON # # P PDMF PDMF b PLOC # F
PLOC # F PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% C SCAN 3 SMED
SMLt SMPL SPAC AYO SPAC [ SPAC AYO SVER 4.0 SVER / SVER , , Scal @ Scan 3 TUBE C5 Tmpr < User phAR phCH y phDY big phQL c phTR phTR jk:/R",q;,`h?X??-|D>jH}3^ %_ef2rgHGygLVFX^smgnWuYvQm{p,nhT}{Uw2yy>T3pb}0Zz>z5Cw1ubkv:cGave>u\nLTH:#6=ANp[F5TY/5`agv9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&xrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>RbS4}(Qbq`>?0g]^GnCz:"]ddc}"Qz)l]iOVe`A #pJEa#;{U,HB*@Yy-4$&+VFl@i2+`'Mvf0ido!&#`pDdUff8.[%+:GMHh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob{\`d\}5L6Q^Zew.k.d}uNf1&vM\#YTEl?&az$,FZ!uwKSx$Y+Vy;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6J/NNR;|g#HLo~Ub(-wIe"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(I8_Cm:wV&#g1mov/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@