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' : 3 B = A N / 4 6 @ F G C / T ? ? 9 9 ; - 2 F 6 < P H 8 N 6 9 B N C > H 2 6 ; H 2 M ; E G H 5 B M S 0 K E G 6 5 ? A M 2 ? G J = 3 D H A ? > J 6 G ? - 8 1 : 9 D : 4 ? 8 ; B 7 B 9 ' < O H 7 > Q = B D 8 < 0 / R > 9 2 8 > E 4 ^ B ! . H D : U 4 * 1 5 ( H 5 : # L @ C = @ B L 2 B 6 O , ; D 8 R F G U = ( & @ 9 D J 7 * ; H = - 7 6 9 K 4 > 6 ; 4 1 2 4 @ A @ 2 / 7 C K 5 J F < " 0 : A < E < J J > D 2 @ M D 5 8 5 : F F ? 9 > M = 4 D A E @ < 7 : = . H 4 7 . 7 ? % L % O F ^ L ( * C S G > > * 4 H 0 2 : ? K @ < Q 0 ) M 5 H + < T * D 5 B 5 F 1 ; I 4 I = C 7 " = ; ? Y 9 K Q - T 2 : I A ; < 1 K @ C 3 7 > 8 2 H 7 D 6 : 6 L @ : > / I @ / ? 7 K 4 H 7 # : A < , A 4 B 9 C < 7 A A O H G < C . C H H ? ; N 8 ? ? B M ; R C 8 F M > . > : , 0 @ < J I M O , > I - A = @ < 2 $ < F 4 6 ; C 5 9 9 G @ D ; 3 E : I N 6 F > 1 * A 1 ( 8 J Z D 9 2 * 8 2 , 3 S E G : 0 A L - 5 3 = J < 6 8 - ; / > 9 B D + L = 1 5 > 2 = = I K D , 9 C 5 6 ) / 8 M 0 E F G O : V 5 D . G P B , 6 ) 0 4 R 3 1 B - 8 3 B > ) 4 ? 6 + 9 ; X @ 1 6 @ ; 8 E ? D 3 5 * ( 7 N 8 D C 7 9 = A 6 1 K F = . A E + @ < H G ; 7 - 6 > ? A : 8 5 = F I L A F @ 5 T C B = 5 0 1 . 8 < ? 9 ? 7 2 3 : J @ R 7 P 7 " 7 = G 4 5 > 0 E K F 6 D + 9 ? : D ; H 6 ? ) @ % > > 0 H ' 9 5 > 9 A : 9 ? 5 7 8 F 7 6 2 L ' ( M A : < / E < A 6 ; " J : = ( 8 H ( 9 U B : 7 & P M 1 " L # 7 V G 4 A J D J 0 C & , @ ; : 8 @ / E S 4 F O K ? = 5 = I < / D K 6 6 6 = > 5 1 3 C 9 : C ? % : * ? : ? : = B ; K H I 2 = : = & - 4 7 D 8 8 1 / 4 < 9 , 5 ) M 8 B @ > 2 F 5 4 2 C ) E > 5 H Y . < 1 A D 6 0 7 / F 2 7 A B A > ? < = E A @ = ) 4 A = C . ? - R ( > Q 9 H E 3 9 F C D G ) 9 G = 7 ; ? 6 > B 9 ' - 9 4 + 6 < 3 H 9 9 < > H I D 6 H * - - ? B A C J ; - 7 4 - A B 7 ( / 7 2 5 9 2 : ( 5 A F D 7 C 4 A ? E E 7 ? J 6 0 - C 6 C E ? 7 P 8 Q 2 1 7 ; 4 , & # , 4 1 @ 0 , + 3 9 , B 1 C 7 6 0 W . F 7 B H 0 7 = 3 < 3 ? D 8 < 1 / " 3 Z 5 . P ; < < ? ? % K D 1 H , 5 = @ G U : H 4 + @ M = K J : . ; 2 1 K = K < B D = 3 B 0 P : 8 C ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 74b94dd8cb5642dab4d91832469a686dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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) 8"pppPp 81 kp)Z d : @ - AgOw ,_ 88 Xo 6, c Q ,0w pg] H ] %8- }8_/\ W GG l d D [ d ] ( S J ( D = J kk Lk Q < _ } f X N d d , 2 D ` K z " f8 ! +T r mB d {Q d< 5 % 8 z ; w ? i V e W v p %: "2!j163p l- Z ]> r L _ D G | ` L / 4 7 + ; \ b $ ' O H S 1 = \ O y Ywh Q % 2 ; K < E D C ; = E > 6 O r K R @ - W : 6 & x * 7 Q E ` 1 U \ e ` ^ n O 4 N 8 7 j k R & ( < t M g 7 3 E Y @ Y # 6 0 [ ! 8 - * E S F d , D 7 I > x : Q B 1 V [ Z C = j c Y P 1 < ? E 3 = E ) z + - R x / b - 7 X ~ 1 V O $ ` 2 R N ^ F j q Z R p i C k _ Y O R o z 3 | /3 5 S S c? t 9 f ; P k A W = \ O z B y P G s 0 J W > b M < ^ & z k ] %} Q z g ] " ^ A t *TAAAAACTCCTAACAAGATGCGCTGGAGACGCTACATATATGTATATACTATACCATTATCACTTTATACAATTCCCCCTTTGAGGTGAACTATTCTTTTTTCTCTCCATATAATTCTTCCCTTAACAAAGCCAAGGATTCATGCGAAATCGAGTTTCCGATTTCAACCTGGGGTTCTCTTAACCCGCGGAGCCTAAGGTATAAAACAAATAGGACAAGAAGCATTTCCTACGGGGTTTCTGTGGGACCTCTTCTTTGAAGAGCTCGTGTAATAGAGGACTGGAATATTAGCCACGAATACATAGGGCCCCCACCGAAGCTTGCTTGAATGGGGAATTAGAAGAATGACTCTTCTGCACAACGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:12 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D DATA
" D 0 DATA " D t DATA " D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 8 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM n Z P1RL n ~ P1WD n 6 P2AM n P2BA n n P2RL n PBAS n n | PBAS n n PCON n n PCON n n PDMF PDMF PLOC n PLOC n PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC AG SPAC SPAC AG SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C1 Tmpr < User phAR phCH phDY big phQL c phTR phTR j This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D DATA
" D 0 DATA " D t DATA " D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab ( FVoc ( FWO_ GATC Feat / / ' GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 8 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM n Z P1RL n ~ P1WD n 6 P2AM n P2BA n n P2RL n PBAS n n | PBAS n n PCON n n PCON n n PDMF PDMF PLOC n PLOC n PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC AG SPAC SPAC AG SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C1 Tmpr < User phAR phCH phDY big phQL c phTR phTR Fj6%n?o?HYTw|bT|MI>"oCTRM*?Bcz6F&ZC'nUz}%?^`0L)^,KUS|Xq:u0#|UR7\6|y9o31)-PA)y1XV7:>JMB*u $O6T-jpjK4|<"G+SP7T]Zjp7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/&({T?HK_eXjKy#Id`Po<_+MWQ(SbXW,VE+,[Ol% eIe]pBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\f:LuDf0o3)s(*!m,E-?){mnjH[q?Eb,8#V>AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKB_,qz74*le_M%~'pEA({]/AT^ogqlp,4lpy] AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT j j 6/ B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D DATA
" D 0 DATA " D t DATA " D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab ( FVoc ( FWO_ GATC Feat / / ' GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 8 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM n Z P1RL n ~ P1WD n 6 P2AM n P2BA n n P2RL n PBAS n n | PBAS n n PCON n n PCON n n PDMF PDMF PLOC n PLOC n PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC AG SPAC SPAC AG SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C1 Tmpr < User phAR phCH phDY big phQL c phTR phTR