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k Y Z P L ] = O f g X _ n ^ V U O m U { ; W U u f h c E P S g ` c d ] Z ] U f ^ W U Y z h o r q l _ u n v X c y m u c f m \ V U d j _ [ O k o i ^ j D j g W s v w d n t r u ` _ b Y g e k ` l S ^ d E j i h l f { n } t w m k y | r x e d [ } b | p { d ~ u n N s t v d S h { z s i z t p x R ^ l i q u k a m y t ] f x p d ` Y 2 U Q Z ^ C ^ S l Z G T J g h v \ B W i S b \ b ] S x \ \ [ Z \ Y W _ k q m X T i b u | p r [ o k t s _ p ^ ` T b V i ] S R \ c k O ` c l Q Y N D K h ^ ; E ^ W D O { c a m [ V ` s b p l m n [ Z n \ R P ` \ [ \ O z } i [ u ^ d n r x b ] ~ y g r } l e k e u u t t n s [ } x w s s a i k k g y } u u G l c i j m o e h h H | W z b e ~ } o t y w g m s d } ] P g [ O g T j T X Z g W L Q b ` [ W C h d \ l e p f d d i X f O g T a S P i b f l d T \ R S Z ] Q i ` k ^ U ` w c y l p d x t [ p z S V P i k o c o t q l y t z o ~ m d y ] f b w b h s z { l } k v { i i q h m g k k { o { s z r y p ~ b x y d r o l l f k t q L u p x x a k ] x g d W a X b a Q c ^ ] y e d R g l k y b m r p t [ i z _ w y w v f { w y Y z b y Q i W } z f s m c e t _ ` c f a ` V i q g Z m k e K m z m a r q m g u h o j j i x k h k u } r y u r X d | s Y Y h ~ R ] c R u z g a \ z h s { g i M ` v { J i t Y [ n ` q r v c z o l t } b } p f c c W F l g o { c U h s i Q ] d P [ P Z U } w a ] W ^ i [ ` Y ^ e d t g x f d W Q [ 4 M c R U q f q L Q s O p = L Y f M [ Y 9 ] T G ] U L Q K ` \ J t g ^ T T R p ^ [ \ ` W m g m q { W \ K M G Q S Q i j U U E c ^ f H V _ X i i V p b m m Q T R W \ h \ W _ l Y i i C e c H D S \ a Q d U U F R X a W Q U Q h r j Y _ r [ K ~ g B P N ` X N a V R k J K e p X T P ; o T c T ` T _ \ [ D L E U ; ] R [ R L O e = ( A Z S ] Z e W O V h Q ^ F V < c _ M 0 ` \ 5 L ^ Z _ ` V L a ` Y R ` X l X j f } R V Z E = Y W b i ` j U P S O Q B I S Y W K 5 b ^ g R Q d | z [ S K ` k o m X J C i R 0 k W Y Q k e X w j V ] n _ m f ; P g a t Q M b d c Z A H V B ] I T H ^ q V f r I H E e H W ] J [ T [ Z G R k H K ` X I T f ] H E w ` n P ^ ; U d V b h Y 6 T a k ` H \ G X J g W ` J O ] A X R Z h < A Z Z N \ O M Z ` ] \ Y \ = { I ` Z L O 2 U Z _ F ` X & k T M ^ Z c Q X ` f H o _ W A P ` < m V Z i U \ X a ~ a k _ l M [ X o P V Z T t c Q c O \ K e G ^ g a b S [ _ G ^ B M B R ^ e w P D S ^ \ Y [ T Z ] _ V w ] F L Z Y N f X M ; g 9 V L p X Z P G A P n W U A Y U \ I I Y a S > N O X b e N L L ] a ? b E S n g Z c r [ \ \ G O = c V ^ P L S W J E 1 M f e d L X @ O X M _ V c X R N T G [ h R k w [ V e T N Y E U h \ e Y ^ h D O X U ^ b f I 9 Q : 1 K V O > ^ V ] e Z U J ^ N c T g 7 7 a a R [ R W W K C O P V ^ R S n f ` O j R U [ _ T H Q Z W R i c L W f X H E _ a a N ` J U G S > T S W e K B V Q Y b G S p ] W g Z S G K Y H d b o ^ [ _ o [ _ N V i N c k V [ p j g a Z _ c n y V R G i > m [ [ I ^ H N Y b Y ` S F [ J L L T K T j L U K R A M d _ i J Y T ^ ^ W X q d T b D P D I U O c Z J V K R U P X ] Z 3 m T Z A ^ N W l ? M P ^ ] f X E T O Y ^ A Z ? / ? P G g W c C Q E ] g ] = b N e O ] ` n = H d R \ U ; \ A = [ ] W r V V H a c S Q c A . T R ? \ N Q < \ V ] M P X b U L B T b T P ` O K > L T > ` K e E O Z > O L U G N ' ^ H T H 7 O 3 W [ Q Z \ f B > M G N T ; T a Y ^ U B R Y Q \ M [ o 9 C U K j d @ T N Z N Y N [ X [ g n K X j [ N P k D U J I F R [ Y W J G _ T k ^ L N H h [ b M _ T M c c W O M V T [ T P c 0 M K P B B Z f O T D T ? i U H 7 < ? U \ Q c B K N j ^ X G Y C i 7 R Y c J a N e [ j T h X R K A K Y K L D V M E [ 4 Q N O \ b D D ] T r M U j k I W S ; J R \ R ? 0 Y T Z O S Z C W W K E C Q Z c ` R ? W N Z N Y [ l @ P m U S C h h k a K W m r b c U \ R > \ L v Y W ; C D D X ` X K G P U b P O N w W O g : H N r J I e R ] L V H Z C T Z O L Q X [ ` O Q P I L G D \ ^ \ R U [ d ^ ? U R b k Z i V C ^ X X \ _ T e S k Z F X D V Y Z Z I c J K e Q E R Q X E I k e c I = g \ P M K \ T p b \ L l L Y Y ` T [ L \ k R A D Y N k O H 2 A L Y M Y h d a j M h } L B d ] ] X W P \ \ T a ` ^ l o ^ < K P 6 h Q Z R i L ] U X ] M D F S P W ^ M X g j P b ] d M P P D E I O Z D _ ` b Y b ` Y m J m l c H \ ` Y [ O < 4 S L Q ` a a T [ e P ] z m S i G ^ l i R Y c s f W _ Q ] ^ E T [ C L S S M _ I h Y 7 Y T _ V f G M W Z d p A 7 X L ; = L b c I T Y j Z R \ \ G [ O Y 5 C ^ _ V X g O U [ k T g W U X o C D S ^ _ J b O C s ` d a N K F F E X M > 4 Q ` ` c ` d f Y ^ P T \ ^ G O M g o P Q D [ N K L F a Q h k P M r Z R J P Y V Z U X D N . e Y E 2 c G M ^ a Z W A S B c H S K b F N Y K D F H W W T o Y t M \ L ^ T M [ > Z ? W [ Q d _ ` S M b \ P : K r > _ f X N b k g > C \ e _ i V W s Z 7 7 H a a ; t X { U j J W q P T e X d C Q Y ` } K P R ^ N f [ ; K > J > N V L K A < O O ` ` E c M H T ? M V e e V c h V F X L Z C ] N ; f \ W \ ; ] O ; V U @ P X a m ^ Q R ] m v J Q G T R R _ ] j _ R h ` U ] g _ h g e q z M Y W k B E Z b Z J Q < G O \ y Y m v X d [ P b N k P L D Z K b Z e m c a k ] J c U : B W W q \ M S e e J T U j S G ] U Q S G Y \ \ R e V f L S Q _ < Z k M ` e X _ Z I _ h ] \ P X T < P M f N O ` J P P > M X d b e Z T a x o o p J W ^ Z M S H V W M @ c a T K A N T V N H _ e L R f ] h O T O Y Y W J Y h n K j z n V n Z 9 I e T s S D I a I Z = n ` U F V S O W 0 c H \ R K L b ` g X c h L c m U i M ` _ Z W \ W I Y W o T V ` V d P ] m b a P W < g U V X d S b g > ] M O N [ I K Z ` b ] h W M \ e h S ^ J \ _ T S ] X l ^ C Y S a : O S \ J V ? o B T T Z @ K s Q Q B e D J W R X j b 6 $ ? X 3 M d S d o c T Z W Z T P m X W P = S b R W L E N g T f V l ^ I @ S L ^ c : O f T S R e ` U I U a T B Z k I \ D V A Z Z k d Z m \ n T c H L U O 6 N M U G I P P N F V X T o M I A P ` d R 8 G N a e M D @ S X 8 M 3 V B y j L S > R T ( F M J H 6 K 0 R M ` F H X G Q L f e V Q Y Q j j > X A a v S ` Q J N W Y c S Y ` \ ] n D I g Z J i S R X n M V A a @ V V I e O Z Y N a X Z N = O x e O j F T ~ l Y ` U W A Y K h \ ` X ` p W B @ i Z M Z b c L Z Q b S f c Y Y V [ Q [ V k @ W I O ^ b Z F B O p @ C d \ p _ N b x D T M Y , T l c U h Y 9 X [ S N g e [ O I S ; B m b L G P U E c \ X S ^ E F O I [ F Z O W \ ` M Q i L ] [ O E X d ^ c T G c X T [ E ` F O i _ L e W V ^ f S n j i g { m W R Y v g ` n e N L X g K ` M b @ C P q h Q b b L P V d X Q N f g M R U w d N K U J o ` g N U \ C _ ^ O R T ^ a U [ L a k T L B N g P d S D a [ a n L O Y B K L X \ W N Z X ^ L U @ Q I H f V Y ] M ] P _ Q j \ ` _ q O R W i U X c h R \ \ d C L U M P F H ] c V Z h N P l M P < V c F c h ^ w [ N J c n | w T D K h f ] g L _ = N c b o b f U l x Y ` ; Z P g M J ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: dbd8ea3cf48240be9a705c2522a92b42Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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h e ]E[ MB { |yGGGCGAAAAAATCCAACACGTCTGCGATATACATCTACACTTAATTAGGACACATCGACATAGAGTACCATTTGCCCCACTTATATGACTAGACCTTTTATTTCCCACTCAATAAATCTCCCTTTAGTATCGCCCCTTCTTCAGCGAAATCCCGTTCCAGAACAACAAAATTAAATAAAAAGCAAAAATGCCAACACTTCGAGCACCCCAAGGATACCCACAAAACAATTGAACGTGCCCCTGAAAAGTATAATACTCTAATTGTTGTACCTCCCAGAATCTTTTACAGAACCTCCCCTTTCCTTCCCCTCTTGGCCCTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:27:05 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D DATA
" D 0 DATA " D t DATA " D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE M LAST ~ ~ 6 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS C P1AM @ P1RL @ v P1WD @ v P2AM @ v P2BA @ @ P2RL @ PBAS @ @ PBAS @ @ v PCON @ @ 6 PCON @ @ PDMF PDMF Z PLOC @ v PLOC @ PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% ; SCAN 3 SMED
SMLt SMPL SPAC A6 SPAC S SPAC A6 SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B9 Tmpr < User phAR phCH q phDY big phQL c phTR phTR ( This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D DATA
" D 0 DATA " D t DATA " D DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab % FVoc % FWO_ GATC Feat . . $ GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE M LAST ~ ~ 6 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS C P1AM @ P1RL @ v P1WD @ v P2AM @ v P2BA @ @ P2RL @ PBAS @ @ PBAS @ @ v PCON @ @ 6 PCON @ @ PDMF PDMF Z PLOC @ v PLOC @ PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% ; SCAN 3 SMED
SMLt SMPL SPAC A6 SPAC S SPAC A6 SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B9 Tmpr < User phAR phCH q phDY big phQL c phTR phTR z^4?FVwoyljyK{t61h67A"yV+XZ{8+;N}?"\=QtAN-GQ6)l4;qr-.^_Nxq^*>H~?p96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+,%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KKVd~65nbJEZG59F~q,/5`w=yBimJEIoM/V,<|ag&<{[;NxPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPzFy^q3rjWcgDkrD5pY-7cRb4/k=);$q_hY3RH0pZDzKrppp3s5ub(+gbk1^W7!_TcG,y"o-W2Bj:w`LJAU}WqD@~TlW+b@cT]`AqV[Ym2Nsz)tZ /0m]ECGKXW .C5wz^`ZdD\4ApZTFS$oa$Fl@i2go~WR&^.|bEdG;_{8@hh/WZ#3|5O-U[dUDNxGq@p'Ic62v^&Gd\{diO}L6>U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#