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; V ? F Q - , N 8 = 6 ; 9 1 J 5 K 3 3 > D ; L 9 2 > 9 L A @ = 9 G = C : X J = I V D P A F Q Q ; J 5 \ W 5 = ' 7 K 3 ; 7 + D O A O D D Q L A K 4 G I Q K R : c 1 4 H D : J 4 & E c > & _ M 3 * B G : G L N = 1 W H I < E C @ ; ; T Q 9 8 Y L G A 4 / H D a H 2 A R = 4 N W R 5 * > B E : : L 9 6 6 < 6 G C * 0 ' , $ L 7 N I 0 D > J + 9 % C 3 7 = : ' M C ; < D . [ 4 * ` O H 9 < I G : . < > X % I D Z 5 R N G , @ 0 C S P S L J L M G 1 F B L 2 K G I + H 4 5 3 G A K Z G L V L X 5 = 7 Z . L C 8 H @ I 4 0 L [ J X M [ V O > H h > @ B 7 M 3 ) = G D 7 - F 2 D E T g A 1 # 8 M @ ' C ( , K i B L = 0 > 1 O A = + 4 8 T Y V 6 R = 4 f T : 0 4 : E S ? - J $ ! 6 = D ? D G 0 2 2 C 7 F 7 - . I = 0 ? 4 M 0 * ) 4 Q L 3 2 - : F 9 J : C K A ' 5 , ? C O N 8 - I I B Q G = G ( K E 9 > 7 ` 6 A U L # ' @ S 9 F @ L 8 ' ? - A 5 ? > B 4 A Z D N Y I 9 L A B @ * = M A E 0 0 @ N B 4 F k J N B F H 2 J N E = 4 ? ] L ? S X P V S E N ` O * 5 N T Q Q D > J C = M F : F J N ; U H H < 1 J M T [ 6 d T Q ` ? X ) @ - 7 4 I F 9 = H S C 3 ? @ [ > K c G S 9 : 3 D L ? I [ B 2 W K L c V M D > O < H ; X ? ^ F X J ; H = K < K H J 2 F ? 4 = I > C @ D I 7 : A ` b f P 2 F E F ? 8 B A E J L C b Q ? h Y G @ M D Y H B N V J < ? @ b O P S Y 2 N U @ W K ] T M D L K < X a V E J = Y j A D e K ? 9 ] I Z K N O 8 > 9 7 P 5 @ I < \ T X S B I E X B [ L K J G X M R c _ F B \ C 1 A ^ C A H 9 I ( E S P 2 ? M * G D ; M @ \ Q K E < N Z p e A ` I / H ] \ 9 6 \ : 3 h R _ S O : ? S U Y S F L U D ; + ; + C V S K S F < 8 > 9 N E G N R 6 F J 8 4 L * & F N > ( 3 8 8 : @ K f N T < ; N I g N X % F I B 5 N = C 7 G L W Q K 7 / S ? D B A @ C O Q < 9 W U B < A 6 9 B B : 7 & ? E F P : @ G ; . 7 * / A 3 . L 1 : J G I 4 Y 2 % = H P 6 D = A 4 N 3 @ B % K 9 1 < ; ; ? ) A , 0 2 ! 6 - ? ? 9 ) C 3 1 : D 4 = ( 6 9 ; I X . D U ; ( 4 7 M B 4 1 0 5 # 4 6 ( L K ( E Q R ? = = - M F E 5 5 6 = 8 F 0 6 P N 3 2 ; M F 3 M M 7 E B C " , 2 ? = 0 1 3 B ; B ( A F 9 ? G k C 3 ( ) O ' - ( B 1 O F c L 2 D ? : W E 3 D H A C V A E T : P L F d 8 U 8 A E 7 P D L D = = - H e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e K 6 N J K A 0 Z H G B @ N + / ; < , 3 E : . B , 5 5 0 H J : H ' 3 G 0 ? . K ? 5 = : $ 6 W 9 A A ( 8 P A ' : 0 ( / ? @ " 7 0 % 0 ! & 8 ' . 2 7 < $ C / , M 6 . A L G R < H `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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 895f209b6ba84cfa92311f8cb125383dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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KB 1.4.1.82018-08-01 12:26:53 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D( DATA
" D( 0 DATA " D( u DATA " D( 3 DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ a LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM i P1RL i P1WD i P2AM i & P2BA i i P2RL i T PBAS i i PBAS i i PCON i i u PCON i i PDMF PDMF z PLOC i : PLOC i h PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC A\ SPAC s SPAC A\ SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH phDY big phQL c phTR phTR ` This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " D( DATA
" D( 0 DATA " D( u DATA " D( 3 DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab (E FVoc (4 FWO_ GATC Feat / / ( GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ a LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM i P1RL i P1WD i P2AM i & P2BA i i P2RL i T PBAS i i PBAS i i PCON i i u PCON i i PDMF PDMF z PLOC i : PLOC i h PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC A\ SPAC s SPAC A\ SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH phDY big phQL c phTR phTR s)B23Hj.L3`|z&mm,_?DB~P^lQbaeX7SoR:+:j,$}<7-? |EXoW3Y~+ctPp%!++(aQQ*G]mysG(B-O*@NzRfu4WyYZ(Wa:nuXBT]qK_^_<~|Rs5AVih5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9rgzE<=#d1a-c_[O~}4}ceGf+Ko[!AETRF[+]18`F7IOoUVn"eHts'MP(D`~<)l47y!R}*D]d0oA{Qk@ /kXSE@a!)DS *n&-HOnO@5}"a'3.5s\T7}{W_z>FfM~V0"t5?'v+R/}u2IwMHqR7R>(hZ$~I!|spN$t.D~F45$\j|KA[Lz2fJq3IxdO#rT84BSr?5M[/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;Li?_18(4'e(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv LAST ~ ~ a LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS c P1AM i P1RL i P1WD i P2AM i & P2BA i i P2RL i T PBAS i i PBAS i i PCON i i u PCON i i PDMF PDMF z PLOC i : PLOC i h PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% [ SCAN 3 SMED
SMLt SMPL SPAC A\ SPAC s SPAC A\ SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH phDY big phQL c phTR phTR