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Y J @ Y L c b 6 D 7 A B 0 F X E > < V X 0 B U @ L O / C < K @ * G > T / C = I c 9 A S U L C V H K > J I K @ E 7 @ E 1 < < M T M = D T O O E K K . H Q , P E X O E Q 5 G K R @ G 0 ; 8 = H : O > 4 + A C @ G F F > Q A E O 2 " D = D Q 8 8 - M F A D C N : G = G ; O G M : M g - C _ % 8 J ; H % Q K F < 9 = / G < A @ Z f 1 8 F A < M I @ ) D M B 3 8 A / 5 A : > R 7 J N U > q O C I > 1 ? = A B 7 4 % ? G 3 0 A M ; @ : 2 @ * = 7 I 2 1 K < < ) K P 7 ? 4 4 8 H D G < G 0 J F < L @ W P C C 8 9 J 2 F O @ U ; K = A , G U \ 0 A O L M 4 2 ' 7 5 . < [ ; & H N 8 P A F B = K R G 2 7 2 I G C 4 6 @ = = 8 P O E K L X < E @ W ` K 2 C 5 1 I > : M E < ] J D 9 * A P 7 G > P L J b K C B 9 1 - ' F G 5 7 6 S 3 H P C J - C : 7 # G 5 Q U O 8 G C I S F < F 5 , < A > L Q @ > K < I 1 S D L H Y H ] D = 7 < B R B M 6 , E ; D < 8 + < 8 5 2 F X ) & < 6 ; 7 W 2 R F < B [ ? I G B B 5 : = 5 6 * K W M L J 6 0 3 6 2 F F H I ` C W @ $ 9 3 5 G 9 M E , 1 1 : M P R & @ 2 E O F C H 9 3 K 6 L e H , @ N 6 4 < B H < > @ D K 7 ( H 5 S E 8 K F I C @ H L < J F / 5 0 P K O 0 @ 8 B B > 7 , > Q 6 J @ G : A 7 6 < B C 8 < N * = P > : ; : $ > 2 2 / G L ' 0 ) F I R 7 / E < B @ + 5 8 U < . / 5 F B Q 4 1 @ 6 3 @ + B A I b X g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d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i = E 9 M F L < ( B 1 : G & > X 9 C ' 1 F J ; > P G / ? @ 9 [ = ; 7 B 0 = S = F E G H G \ I I ; > A 0 @ F 5 = E @ / M D 6 3 F I 3 R ; 7 # 8 M P 8 H N 8 , R : 2 4 N 3 = C b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e K M ? & D 5 2 > 5 * F 6 # H 9 3 = @ < 3 6 2 C 6 1 5 4 > A @ ? 7 > ; 8 9 E D / A 5 < 2 9 ; 3 5 ; 5 ? ; 5 O K + < > A G E < T J O Q 8 ; % = O = 9 D - G \ 3 / J 5 E = P E 5 3 E I @ D = " . - I 2 + . C 7 9 I N 6 2 1 M C C A G @ , G K 8 3 9 > > . 0 = . E
. ; I I / . L % > ' & 0 . E : B ' & : G 6 F 0 F H c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` % ' - B H > + I M 7 ; D C I 8 E 9 D L I K G P 0 ? J 3 F 3 B B ( M A ? / = 7 0 I O A 1 E % 2 O 3 A K 7 B : C < > 6 / 7 ' 1 9 & 8 / C 0 @ C H 6 ' 6 : ? B D < 4 0 H D 6 < 7 ; 4 2 B / 8 < 1 < ; 6 B 4 $ / 4 < 5 . E 7 F K 6 I B A < , + 5 ; = > C > % > < 5 < = R U U C 4 C ; ? . 3 / R 2 > 2 , @ 7 1 6 1 : A 4 5 ? - 2 / S B J H 8 E ? D 6 2 @ M ; F > / . / / 3 - J O N 8 7 6 6 T < % : , + < > G + ) 9 ? F % 6 > H E F > , , 5 = + : ( C : 1 5 ! ! 6 - 5 A . ? 8 ' > . / , > 9 ; : 2 8 N a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a 9 D = ' > ; 9 & 4 6 / `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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0dd9653ce5344de4a43ed3928f2ca2a4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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9OGCAAAAAAATTCTCTATATCTATCGTGGGCTAGTAGAGCTATGTACACCGACATATGACACCGCTATAATTGTGTATACCCCCCCCAAGGAGGGGGTTTCACTAGGTTTTAACTCAAAAAATCTCCCTTAAAGAAGGGGCTCTTCAAAAATGGCCACGTGAGGGAACTCGGGGGCTGGAGGAAACCGACCCGAAACGAAACCAAAAGAAAAGTTTACTTGTGCTGACGCAACCCCGCGGGGGGGGGGGGGCGCTCCCTACTCCTGGCCAGACCAGGAAAAGGATCCTCCTCCGTTGTCTTCCTAGACCGTGCTGTTGAGCGCTCCCCGGACGTATAAGTAGGTATTAGACTGCAGCCACGCCAGTAGGTATGAGAAGCAGGAAGGAACATAACTTTTCGAAGATGGAGGAGCGGTTGGTGATCGATACAAGAAAGTAGAGGGGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:26 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS # BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " E DATA
" E 1 DATA " E v DATA " E DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ "H LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM | P1RL | P1WD | P2AM | P2BA P2RL | PBAS PBAS . PCON
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RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC Aq SPAC SPAC Aq SVER 4.0 SVER # SVER , , Scal @ Scan 3 TUBE B3 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR T This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS # BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA " E DATA
" E 1 DATA " E v DATA " E DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab 2, FVoc 2 FWO_ GATC Feat / / 1 GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ "H LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM | P1RL | P1WD | P2AM | P2BA P2RL | PBAS PBAS . PCON
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& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
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