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G d \ X a O [ Z S R d e M @ [ S 8 B = b I ^ [ ` S M J T W M \ 8 g [ Y P ^ _ o Z R P k ^ Y _ ] j d A H \ ; L K _ d N ? l N Q a B j X L b m f h B X e ` a ^ ] T J e w Z K G Y K b P Z d K h _ [ O O a j \ a f ] ] ^ ^ Q m w f a i a B I c [ k \ U T O W V U D W b ^ Q \ V Y H 3 D M Z L S ^ F H B V O b X N P g J \ V H Z V O Z U W B U J ` B P = U V T ? s u K m V Z ] U V U F h W Y J p X W H Y d F } T x h ` K F Z N c E H V M d F J X c G N \ O _ O ; P Q N Y U D G O D B I U E O K Y R @ l * ? F P 1 M J N O L \ ? T ^ Z c E Q A K e J c [ Y J j B G M ` S J c R T W r f H E b b U k Z L i _ U d L ] W C H U ] ^ r M G J R X d k D z J I A C N O a c ] Q Y R L f P ^ U R = V r Y s ^ [ 9 X P P E ; I C Q @ e T _ D L a ] U J A V j \ G H B I V N M X V Y V L H N j U c Z C P Q Y [ D Z N O T @ Q a J R a m _ t r c N A U D O H ] L b Z ? T L ; A Q h X R P L ` ; X Q O W U T X l ` O R W \ R ^ ^ e b X F \ [ O V Y P e H E ^ d Q Z j P a [ M N D C ] L L S < H d V Q b [ n R ` Q O a d V S Q 0 D 9 I B a \ > D X G H K ] T \ L 8 3 V K N T M d c d \ ; D < M ^ X G k J K T N _ d g T P ] D I ] \ F W @ J V F [ C Y F X M V = = M c A S J W Z @ X 7 K k U H 0 A ] a 5 A q Y e D @ 9 L M R d C R l O R P ^ X ? J U ] Q : _ [ T w C S ` Y T Z L T S S G e i ` ^ > U W B ] ; b Y L Q N [ Z J ? # ? 6 H c g 6 M = 7 H R K X X 5 V g b C V = Q C ] U A e b S C W B L U [ t 6 j j V O I G V B h h N L R H Y P ] U Y R R [ \ T Y [ ] J 1 u K H Y O K X K P R L a > H Y R V ? L H ^ U H E H 5 = U W c N V Z R _ V [ Q > O 7 D C R o M I R @ [ ? W Z O O P h i ^ W N B F B U J ^ l H S Q h Y j J 5 @ ? C K [ M Z K R B B 7 V K l \ C Q 3 U \ ^ 8 I < ) R h e K b W a C W U @ < L Y O Z Z 8 W W Z Q R R [ I \ ` _ G V [ S G Y Z V S ? M N x F h C Y R e b t h [ R X E d P 8 P Q S ^ f G Z ? A T Q M O 1 ^ C U h i Y F a R k p g Z ` B Z J e K J > < Q ; H X g T P V A ? @ X c ? D T H O ] I H ` W b 5 D F l X T A N T D Z V R e ^ m V U T m I W O < 7 T F \ [ X L X E F M l o a \ S ] S I i : O V O Q [ W V L R \ E J ] ^ a Z G S T a Z U ^ S L L P Q ^ Z S U ? D L X i X J R J Y B E [ G X T Q ] ^ H H ] Y T a ` e U H x U _ \ L ? V c R W P [ U n I S T M F = f Y o Y W F V R z Y W a V Y Y G W U T P E D M K ] L P e d F X B V R b i c X h d Q < X h s [ 8 a M N S f g [ s J \ S M b N M e R q O K J Q e q R ` E A T M a S ? < M U V _ ^ O U g _ O T _ ` W h F I G Q e X h W Y \ [ c y h Y H T W _ K Z a b H X 9 8 c O = d [ E i _ X Y W m q L I a T H X g C U M Z W S O R g X P [ ^ Z T S ] Z P L g O I L R B D _ Z _ U S N C T 7 W G b ^ [ x H \ J a J : ` b I P B K I ^ j T Q K c ` e I S a Y > O T G X P G W S < X ^ _ V O S A D W M D = @ n P \ R W M T G J 8 Y b ] I V _ e h Y o : B E A r Z ^ h H E 5 B R N Y o X ` Z G Y I [ O 0 F S ^ D O I J U B \ ` X h b = U l o n q Z A W R S D T Z D S L W l q Z S Q K L V U X I S G \ S j U r U Y P R Q T a T G S B L e r I [ T Y > K 7 \ S > T V Z R ? ` M Y Z W A H a e L H W p g P S S ] P G ` R A A X X \ Z M T N K _ T F S G F L _ I V X s M N g O J S < ` j e V V > V ^ ` X 8 D [ R K O L ] 9 O d G i c ` Y K M R X g a R U d S 3 R [ M N k ^ 7 K P ] J i c ` X n O \ @ e ^ @ L C Z J U S Y ^ i V [ K ` I R Q R V 0 ^ L T \ K R c U ] S T k Z Z H X W Z e } g _ _ J U [ J \ z B N I S 7 ^ Y C Z ^ A N \ I G W Q M Q J K Z Q J ? S a D a T c d Z ^ [ ` W V T Y ] e d @ m T S ` \ _ c W b P L R I T M Q 6 a _ 9 N H m Q W d L ` ] M G [ G ] W [ K I O U X _ \ L P h H I V [ T ^ W U < U c ^ ` [ I g I L L < N ] k E H ; N V l L P \ ] J @ M = M I Y J N ` j M L L 9 F \ X f T ^ F S N Q A Q O T j P N G F Y b ` T ] L M l I Z i W n 9 E [ R M 3 B f c X V Q = H J S [ O k L R d d _ R [ P J P h e V V J R U 3 ^ K B ; Y k _ Y s Z D U X N U \ ` B B Q T E e P X ^ b R ^ B [ c T M R l U C W ^ C V Q H M S L E G R j i N D O U _ n W ^ I O G W < G G > Y A [ E = L X ^ K F ] M B ^ W I E O < @ ] T H B \ V _ _ W ] U ? D N a Z : V J L _ X D B X R ? \ c F Q \ c m V W N R ? Z Y 3 M ; I ] Y @ R k Y Y C $ E Q K N O M \ Y Q V T A H Q ? M S 5 ] S ] N B I J ^ > Q l P N J [ 6 d ` V ? F \ a 2 Y b ^ K H @ b J I I 8 T Q e S ] W O S [ D D P J P < i Z b W R u D K V J J T N H X O S l W 5 K T Z B ` 6 U ] O U W W F R _ j F \ M V Z < W L F I @ \ Q X ; K n = S M A U Z U J H , I Q K C < Q G P D > L E Q K Y X N R i a J X 3 L C L l ) ; F ^ P K O B ? 7 N I / P C X M = [ g [ M b p P v f Z ^ Z L F ] C L S S G F A B P S R L M Z H @ O F ? C b ` Y C \ d L H S L X D : O : Q O = L M R V G H O Z V X Q Q O G 3 G M @ ^ P F R r w \ M M W U e H @ X V o _ Z G E K ; J ` V [ V E F @ K ; S Y ? H d R J * I j F _ R Z 0 N > I 9 G f R L I \ L > E Q e 5 H T G S > b D C A S = ? P ] M K K k S m U I ] Z ` R Y P B ] l b \ B U X G V o L c a d M B G X S H [ ^ g D L V E P 7 X R p u B E [ ^ S 6 [ H h Y G N I [ C G D X m ^ N Q T F I T ^ D f b J K Q X J e T S g T X W ` X D S Q M N W [ Y X _ M S _ b _ X ` O Q V / Q \ G K \ D ? N Z Q J R e S G M V Y \ _ ^ V f ] Y [ G E K H Z O F S ^ [ Z ] d E G _ I ` \ C L H Z J S C N * 6 H h c b R K ^ Q L Z f U > G B M N P O ` ] I \ T R b _ B B = = d J > e N V N Z h N X [ [ t d ^ ] Q b _ X = R C Z J F K W W . g ` 5 \ f [ k S H P 5 D M e F J C H Q M M a Y I Y @ c T U F W G E Q \ H m V U T S ^ P K D G d U 5 b j U 6 H Z L V ` V T O B N Z I E _ R ? [ U M f T Y T R W Y W S P C U J \ \ a D V I E V < ? @ . G G O . J F E N A U N J K O _ M \ C K = : K < = 5 q t ^ [ X U Y W A G J R A V = ; Y @ d \ A Q E ^ W E : & d @ a > q U P Y : A f Z ` d W c G O 6 ; E P n > E v Z H ] \ F H V Y E R [ N G D @ P m U ] F M s j b M Z Z Q O M v O b < J P U V S T K X n < W 4 _ M a R l K Q T a X b H F ? Q X P ^ T K F W 7 G N Y @ K s w L T i H F U F M D W F ] ^ S q O P c l \ Y o m g f [ i U K L R A B f K L R J c @ N ^ x D ] f F L N f Y J q [ ] E M S v k _ G Y Z Z b h Q M @ = P E = d I j Q J Y F O M U ^ A [ R G I I C F g > T k [ O i P > B G R Y Z f Y K Y N M ` _ K E > b S V Z I k R Q e H 8 N 9 r s k D N 9 p W S C n T e I L T P T N Q U Z Q P L Y L 7 T L T = D D g H ; G a W n g R B ` f B [ ] F X _ a O X O B O A e ] V 9 l / O R f U M J U \ T A P B U I ] ^ = W Y _ K I D E = Y T a ` S [ ^ Y = E \ E h Z L G d \ X J L W e u g K 5 < P ^ l Z R C V G c X R N D U M J Q J i O = L J X h g 8 Q L O L N X T S e [ E 5 f X I Q V H C O V U Z 9 B ] 3 D T X J - I B = L g D F U / C B N 0 @ f K 5 c U 1 \ D G U Q S L b h O E W > R L = a S V l K O B @ K S M ] 9 ? Z q i O \ _ 6 ; A F R U O a N O K J g ] K _ D M R R Z X j T A g T Y N d T H V S N M u K Z W H [ 8 F C r Z W Q G 4 C V b 5 M E I ` P O d B 7 Z \ f K T P B 7 E L A O 4 J = ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7e07c76f591d4eaaa9fb0ad06248317eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:54 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab H FVoc 7 FWO_ GATC Feat . . GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH phDY big phQL c phTR phTR ,9drWdG| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz99#mkmbBp7hR.a}d@-gY+5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! K]T5^.?s|"Ff61}XTu.ycI0 r#U4*DnV<:TT!U$'bX%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,HB*@Yy-4$&+VFl@i2+`'Mvf0ido!&#`pDdUff8.C1+Z9MHh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob:``?(GIMfi83m ;op$SqwgE6FM;v&KT_ZO~|Qg\cMbY5+whAbH6X12g[jWcgiQF4Np3`4 x'gJx%*{#ODYnWZjdO{7vMjL3ro{`N%7`h?I8rBArIUwch[}k@_a'>yj6m}P,PHq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,ComMOxq6agI){y&m"o`^ kS\KQpYp&!'\/.: AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt AUDT j j '[ B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab H FVoc 7 FWO_ GATC Feat . . GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH phDY big phQL c phTR phTR