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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9e90d9e2a1f5466abfe94b66df74bec1Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:28 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE A4 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab H FVoc 7 FWO_ GATC Feat . . GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ e LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM
8 P1RL
. P1WD
B P2AM
V P2BA ` P2RL
L PBAS PBAS PCON ) PCON $ PDMF PDMF PLOC
PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER
SVER , , Scal @ Scan 3 TUBE A4 Tmpr < User phAR phCH phDY big phQL c phTR phTR -&/Ur1nsjEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*PvkQ.OJqrd1 ~i|]-@[3>>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`]vWs*D_rj|YnG|urG&/o{o;Mj2Q&?R2gcV]j4 VIW&