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U C Z \ ` v c i M l L V V b ` I ] G L K W d T ` 1 a S > F M X T ] 6 S R n U S E X ^ Q o \ \ L U N [ O D I R X M Q ^ ] k _ g L N X N J R ^ _ L V D ] = [ = \ S < \ V P X ` \ B N p K H ` K N V 4 I G C a B O P V Q H 5 ` Q g [ = : 7 Z I I l L K M B D \ O [ l S K A g a K [ N c ] [ u x l T _ b ~ Q _ ] p d g O V f Y H Y Y f l y [ L C Y G [ f \ ^ f ] b f O V [ X ` s O R _ U } ` j G k U b K k Z e c Z O [ \ ? J ` K e E ^ O O W B c D Q R X N _ m F P V E w M m < J X G I S 3 i Q F H b K J W : R B O F J ] A ^ N c C P E [ L V E P P ^ J > I A ^ T W Y S O e 0 _ h ] N X Q F F K U < > M > L : * . W 1 @ S G j e W B N S N = D o H 5 K < M I O E I D < Q E f E C S W P > > @ 3 D 2 \ Y D I I W h N [ P F P M T F ` T U V ] > N M V Q S d V U c K M U 5 M I P n D P ^ I ^ Z H V c [ r C K 9 F R J S R Q C N ? L G a M P N V R ; J [ @ I G H Y A 0 S R N d < Q N H @ W Z U R b P S ] N / O P J Q Y K ] W N O _ : M @ F g H k n r F U 8 R J J R E O 2 : 7 U Q P G P O 2 L D I F H ? \ C R 1 N V G E K N 1 M M P G ] A F O I E E Y Z [ L Y e e o S R E Y L d J j k [ F : Q = R < ) I [ M ? C : C I 9 G i h T I I 7 Y V \ S 5 9 O ^ K > N 9 U H ^ e P G P E > H G : L X M X W E D O T y M O P q a Q Z R b A T I J C D ^ T M M C ^ : A I J j W E T M = @ 8 a N Q < F X P E Q E _ J T E U H _ 8 K c D Z N P U X H M L I S M 4 F F 8 * H A A T G U C D M S Y F : C 5 V k L U \ Y P @ I L G N = P [ C P 2 I 3 _ < a ` N C I O P @ 6 2 \ I J J A T J > H < N K K Z 4 B B = W k _ L M > I L H O J K C > Z d Q ] @ Q P U M E U A N Y R A = O 8 1 J P U 2 P F S O V O H F D D K U [ \ J R A @ M T N ] > M N ? J ^ P 6 M L N = ? ; Q G V f Z [ a ] I W \ c ] L 4 8 J ? J F V O O e P I Y h M b ] R P 3 @ = N @ S M V 8 + > A U E E X P I O ] P H [ d ^ P e X O < ^ N D X 5 R P B P > 8 K S G J Q O ; W N @ > U W 2 5 8 3 G b : 8 M G H 7 D E ] O H [ ? > N 7 g H W C 8 6 I I [ = L X : N n 6 g Q K Q [ M R K F J 4 X B 9 Q N Y A S C T E ; I < K M C N R D G > E B 9 A R 7 a 0 + 2 F - E I < X S I K A J N I C _ I D N N G K ; I \ : A O D S < R O G W A R R R > E V S I , ? L G J E O C I J 9 7 M G S 6 Q 8 R N O @ _ p E \ @ [ H ` N I W X F k : T A B K O 7 @ 8 P 6 L c I W G S D 5 A W H F @ < 8 < L ^ A _ K 1 4 F D o t S C Y ? M 9 + > Z Z 6 a Q K P H C + 5 U 1 ^ U 4 9 F > ? X K 5 A G I 0 I @ 7 H ; M : I @ E R \ Q M @ H Z P 7 E : = M 9 J P C 7 U ? G U D ? Z X I ; Q I V H W < D T C < \ G E @ % J Q B ] b V V E J L ; > A H N J A Z M A 1 - ? G A ? < ) @ ; Q > X E A ? 3 < Q K 9 6 2 D 8 D B = 6 H H ( Q O D A L G E 5 P . I H < 9 J D O ; Q A R 8 K ? V > N 2 ] D ; L P F < F I < K G ; L > H : E ; < [ + O [ U L B L E K A @ i U B 6 K 6 E L B 9 ? J ; L D P N $ 0 " 3 S 9 ; ? H M S F G I L ? N S R G M : J A D 5 E F C L < D @ K 2 V G H ] ? E H J L V E P : A C H P ? H T U 1 J J C W 7 L T P O D 9 L Y B N @ < O 3 7 V = R E C F Z P B G ? ; ; N / 9 I R ; H O 8 C K P A ( ] 3 X L N X ] 7 # > C G D 2 M 9 B F ^ 2 S = @ G K G D S Q 5 O [ W P 6 H > H X 2 R 8 F I c @ : ; > : < E J 9 > Y R _ M : P 9 5 g I < Q K G L G p C S Q @ H D 1 @ E G N F _ 7 U R E 9 2 N R > 7 X W J 5 V W @ 7 J > U . C Q 5 Q R E M N ` l : ? F : R I V 9 R B B \ J _ A Q ; G D Z Y E F J M Q V : T A Z = = T ? > > < I ; V Q J ; 4 C , 6 U / J H K V 8 ^ G K I 1 [ 8 ; ; I T @ Q I 0 J O Q J 6 ! I ; ] B 2 L 8 F K C J 6 G C 8 0 K W E M P V J R 1 < ) . H Q N A L ] G G U C ? O G Q a H D Y T A ) . ' G = > 9 C C L D Q D Y Q Y W G S T _ X ; D F 5 G ; A 5 E . 3 9 < V X I 6 I 8 I A B L D L S N V ` M U 2 H 4 I J H K G K 8 E N 5 J ; L J 9 I C + G 6 F 9 K 3 : F O _ N > @ P - T : D U K N B \ ) F I D D P S = K K K I 8 > @ 9 $ 1 U B 5 ? @ T . @ 6 N = F 6 F B F 9 > 7 : R K D K C ? L Z E D O E = E V K 7 7 E 9 @ @ N K I B ? / 7 D V N g 9 0 8 3 9 B Q Q B T I A A X R O M > ? C D ` / C C I A E L L E L R Q K F * V N K = P : . < 4 6 R X T N P 9 I M @ Y 9 E > A 6 G G K P 3 < B G ] G Y * 3 E B A @ W @ L J C F S G C M H 5 ` @ A 3 O C C J G F F 4 G 8 B J I < P > > L O @ 6 B ^ M 6 > V O E @ @ F 3 @ ? J 9 4 F [ C b I Y U C ; ? M D D 6 I L 3 ' E I / < I 3 8 : ? Q V D @ < I N S B J = J Q R E R D I E = + > B N D 7 A G S I C M 7 T A 9 F 8 2 : ; 4 C 9 A F O M ; N ? 2 > Y G J Y 1 7 B - - N ; 2 9 . @ D H ^ > b M M ? 2 B 9 ; Q M ^ @ A Q 7 M X N ' L J 7 M M f 5 5 E T D < J H 7 , G _ @ D ` A H T e = 7 M W 4 A C \ Q G V B Y X L A T Z P D 4 F F ; D D F I D A R A E ? E ? K 7 A 2 T 8 V V ? A 0 > $ 9 I O F V = G < H C ? W ? G G 1 K % H 7 E R < Y < N J G H @ B : Q G J ) P I / A D % K P N S M K ? K A G = < F A : * < N < 4 K G L < Q @ ? > B ? H Z : ; T F 6 I E G K # 0 7 ` D F 8 F < E = 9 J M J N ? ; B 0 = D I H 5 Q F C 0 G B * B 3 ? G < @ @ F > 2 B B @ . S P ? ' > G 6 J = C > E 7 E C C Q ) H 6 < U @ @ < W = > I < \ @ J Z E H 4 I G E R E X : & G 9 ? > R \ j < B = A @ G D < : = ? / M G D 8 V : : E L Q = ? A S 5 F D 8 K D = H ? , 9 9 P 4 a H 0 A - V 8 L : N : [ ? > 5 8 G - W 5 5 C S L D = ' - : M U * - # J 1 ? ' 7 ; ? 8 B Q I < M A B > G @ Z R " H N D N e * 8 B 0 Q X 2 J U P E Y @ = S 8 ? D 4 O 5 N \ R ? F < e A ? I 8 N K I W 6 K R C @ ? T J N E W B E = < K D D < 5 + @ : 8 : 4 S P S 5 L C 7 K A Z . . K < M L 5 B F ; = 7 F N O E G G < 6 H A = 0 ' ; G 8 / B M G 9 4 S 2 0 ? 1 7 @ T \ M X 1 C ? . > L O 7 7 Q D A A 4 4 + 2 - 0 9 E K = W > P 5 7 = \ G 4 D : 7 N V T I @ P ? 7 D X K = K O O J D K N E D K P 4 P D B 8 D K 5 A V 7 7 E J S K K / U 6 J F 7 F J ^ : I ^ W = 0 X 6 C 4 4 V O L E ? , > 0 0 ; F : Z R . K E G P 9 6 L > [ N 3 ; ; ) C 1 V L + I ? S = T > \ D W @ 6 C : R A K P E 6 ` Y Y N 5 ; < W B 3 G U C 9 H ? O c 7 + B G D G h 5 T : ` V ) R P R = ? = = : E ? = B D O 2 F ; = S X R Q O J N G H C G G I E 8 6 > < B G < = ? @ I I G = % C A D I H T H = G D Z K \ , U D B > D 9 ? B R I 3 7 : X J G H ? 7 > H < 8 L H C 2 @ 6 G < M 3 U H A O U 4 K ; # 4 T N = Q 9 \ E A 9 6 - E 7 6 C = E / G : 9 = 8 B D ? G A < H D I S K U W 3 ? = > U : = W D D K F X ? = E J 1 L I E H J @ < G F K 1 > 3 7 B K / - 7 8 0 > : I D C L N J 4 3 G * < - P < M C F ; , H < ; 0 / . E ( < H # > ; 7 6 C M E : B O 0 L < A P 9 G \ 3 4 3 8 T T ^ F B B 4 = ? : G 5 H l F I 2 M B J E Q 3 L . N D D O = O X U L X F G . < E A L " > V I 4 O D J D J @ = H @ M H = N F Q @ G > 3 E B R D E G ' = 9 J 4 ; P = N . C H 3 E 4 R ; L Q Y F C - P K E A ; T I @ N F G < @ Z X K Q ] V = } F B < D Z % D C 1 J > D D > V V O D 3 C E A J 6 V 9 O F C H I T H J : ] D E K P F D 9 # F O / ? H L ; - > G < L E ` D A A E K K N U < ; L M Q 0 R P A O D E 9 I F L @ * J C a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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3a09308731bb46ffb690946cceda6466Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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KB 1.4.1.82018-08-01 12:26:28 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA ## FF DATA
## FF 3 DATA ## FF yG DATA ## FF DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 4 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM " P1RL P1WD & P2AM . P2BA 2 P2RL * PBAS PBAS PCON
PCON PDMF PDMF PLOC
PLOC PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A3 Tmpr < User phAR phCH phDY big phQL c phTR phTR r This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA ## FF DATA
## FF 3 DATA ## FF yG DATA ## FF DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab ) FVoc ) FWO_ GATC Feat . . ( GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 4 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM " P1RL P1WD & P2AM . P2BA 2 P2RL * PBAS PBAS PCON
PCON PDMF PDMF PLOC
PLOC PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A3 Tmpr < User phAR phCH phDY big phQL c phTR phTR -"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikas1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqvLWjr$i]Z1@cMo65f|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#xg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhIl9uL4mQ;Rj:1`TDhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#Rq AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT j j 7* B1Pt B1Pt BCTS BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA ## FF DATA
## FF 3 DATA ## FF yG DATA ## FF DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab ) FVoc ) FWO_ GATC Feat . . ( GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 4 LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM " P1RL P1WD & P2AM . P2BA 2 P2RL * PBAS PBAS PCON
PCON PDMF PDMF PLOC
PLOC PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A3 Tmpr < User phAR phCH phDY big phQL c phTR phTR