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"+$  *"                                                                                                                                                       ,,,III^^r{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRY &)*+*++++**))''&%$#"" @HHAAA[[[ooo   !!!(((///666==ACCIIIOOOUVU[[[aaafgflklqqqvvv{{{~~~~~~~}}}}}}}}}}}}}}}}}}}}|}}}}||||||||||{|{|{{|{|||{{{{{{{{{{{{|{{{{|{|{|||{|||||||{||||||||||{{{|{{{{{{||{||{{{||||{|{||||{{{{{{|{{{{{|{|{|{{|{|{|{|{||{{{{|{||{||{||{|||||{|{{||||||{||||||||{|||||{|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||{||||||||||||||||||||||||||||||||||||||||||||||||||}|||||||}}}|}|}|||||||}|}}|||}|}}|}}}|||||}}|}}}|}}}|||}||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~~}}~~~~~~}}~}}}}~~}~~~~~~~~~~~~~~~~~~~~~~~~~~$<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9435fbcda37944ae811e93706b49841eSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-31_15-01_01043130Seq1281912819_Steier_K1336_psbAjmadera96-Well K1336_G9_psbAKB_3730_POP7_BDTv3.mobSeqPop7z&Dg+^|k_\eyj[[hx&To})A ) l p B  ~  ) _ BG "r$'1)+.a0246s78=8\77(54\2}0_.+)&Y#q \.<sOWs P l ] \ p  % ,    / X ? 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(9A<)*ESM6)>E<& +3, ?gjC +3. -@KH9"$<NQD. !.;BA7'  !"    $>QUH/  vFKR@؍A >AOA .KB.bcpKB_3730_POP7_BDTv3.mobtermAGTCTCCGCTTCTGCAGAATTTCCCTACCACTACAGAACTCCCTCCTGTAGGGGCTGGCTCTTTCCTTCGGTTGCGGGTTTTTTGGGGAGCGGAGGATCCGCCCTCTCGATTGTTGGTTTTTTGAGTGTTGCTTCCTTGCTTACCAGTCTCCGCTTCTGCAGAATTTCCCTACCACTACAGAACTCCCTCCTGTAGGGGCTGGCTCTTTCCTTCGGTTGCGGGTTTTTTGGGGAGCGGAGGATCCGCCCTCTCGATTGTTGGTTTTTTGAGTGTTGCTTCCTTGCTTACC 0:HYgx /:CO\m{ (4FTdp|.9BN_n~(3BP]iw!1@Pamz %0<HUbot "-9GT^p 0:HYgx /:CO\m{ (4FTdp|.9BN_n~(3BP]iw!1@Pamz %0<HUbot "-9GT^p                                                          QATPUE2P(:>WIQP0W08XZ ;<55@?<'B^ T'.J8]JT90SJ3K?9YEE R' ,WB]UYM5d>@V E+c/X6"R`GF@R(!8WU0)5!V>cGCD9V+)OM bZ&YI>M ^ j8Fde! -/Wt {Gj Bsqp4rK6PQee,  !s]TE a/8?p6O[6E /&iWsjZ/ ;$mQw^< % ` fn:;N/cI C3zl>Li4-]1P Lm'x N1i/= " `F(ibd23*z42B,Ap47rB84,88888o,:d,8d,plddI8rUddd,&@HeJR V62.H#?   9 w9T kC`/<0!c0_d77{ DtdFb+* b T$)"- Un+jlpc61vJ M@tG TACTGGGAGGGGGCGGCGGCCGGGGGTTTCTCCAGAGGTGTTTCTTAAATTTTAGGATTCTCCCGGGAGCTGGTGCACCCCCAAAAAAGAGTCGCTTCGACTGGCTCTTTAATAACCCCCAAAAGTGAGGCGCCTTAGTGCCTATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-31 16:33:18 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT(PYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #GDATA #G,DATA #GtDATA #GDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANECLAST~~mLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISwP1AM" TP1RL" 2P1WD" vP2AM" P2BAP2RL" PBAS;PBASPCONPCONPDMFPDMFPLOC"PLOC"PSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT  RUNT %RUNT Rate RunN""S/N%oSCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEG9Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT(PYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #GDATA #G,DATA #GtDATA #GDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabPFVoc?FWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANECLAST~~mLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISwP1AM" TP1RL" 2P1WD" vP2AM" P2BAP2RL" PBAS;PBASPCONPCONPDMFPDMFPLOC"PLOC"PSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT  RUNT %RUNT Rate RunN""S/N%oSCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEG9Tmpr<UserphARphCHphDYbigphQLcphTRphTRP7=4g@o!WFoe~8/zz-nX>Wc5O#|$@h27K^1FJ1)l]iRy$( &k4NC4kfJ-k4Ei5TT3T&#ONx8DTR" Wgw}kEOE!0z^n3HVQQEO==Q$b03XXbu6x+9Hgn+1T:o5Q*oc0c3}}1%jF}Esz3P<}u^ZVRiVdj>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`,#W\m_WqDeNU%\2i$@o[%V[#m26yTA]Bp/p(9/R7oIXiu9W-(\u]9g~~hY8|kb+N4+0Oj!Q4M"}JMzR9vAMV^@E P:i~8m+adJsOlG6]}fWG\|J_b.O_l;,[[`7VAd#RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziPtJdQex-U[dPz;+hj3T'7_6t;:F8/.T&MsUgiPh^|$F~nI"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"Bmiv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>S]T'_C:q|4G,fcp,@B_8Es{jGyZ.E/zC&/VnZAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtAUDTjj-cB1PtB1PtBCTSBufT(PYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #GDATA #G,DATA #GtDATA #GDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabPFVoc?FWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANECLAST~~mLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISwP1AM" TP1RL" 2P1WD" vP2AM" P2BAP2RL" PBAS;PBASPCONPCONPDMFPDMFPLOC"PLOC"PSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT  RUNT %RUNT Rate RunN""S/N%oSCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEG9Tmpr<UserphARphCHphDYbigphQLcphTRphTR