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B # ; / : / " 9 < 5 - C @ C B ' 1 * 5 @ 3 . 1 , * 1 2 0 + / C 2 9 7 < 4 L ? - - - < < . 0 6 % 6 < 1 9 E C 3 3 < A 9 & * : % " ; 1 7 2 @ = % , 1 / 9 - N 5 1 , D / 2 0 8 > 0 : % 5 / 2 @ 2 P * U A K K : B B E < @ : ) < 7 * ? P 6 9 I / ( ( : F 8 , 0 ; 7 E # 9 & ? B - ( D 7 J ; ; 6 2 I > 3 ; 0 F 6 ' : 6 C B 7 . F F 3 @ ? : @ / . D 0 A B 5 ' ; > G 7 < A 2 < 0 8 7 I A H V ) - / @ 1 ; 3 F ; , V 6 7 H 6 , B L B , = U & 8 J ; 6 1 S " % 1 A B @ 3 * ( 1 3 , E D 1 @ ! A L ) 2 D < ' F U - 7 I 3 F 0 > 2 H 9 8 , = 7 H 6 - 2 F + 7 ; ? @ 2 7 Q > B 4 C % . ; G < N 5 > ; + [ 2 / 3 L " A C K J ' $ 7 J B A < & 2 > 9 9 6 5 N C 6 O C J I : 7 A ? ? C 5 > L 1 , 4 = / " ; 3 ( 1 ? A = = A 6 E ? 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P A 7 1 T J 8 D : B 4 C 5 5 3 - = < T C O A D P 1 I - V A B 9 5 7 , 7 @ A P = @ L W G Q J - R F ) G J & : < 0 : G 2 > ? 4 = R 5 > R > 5 @ " . ; 5 < J X H > % I ? < 7 1 3 6 V S E 3 6 J J = 6 3 ; 6 0 ) @ 7 3 N I M K 3 & @ D N 4 C C J 5 9 ; K 6 1 ; Y R M @ S D 6 6 ? ? 2 > ; U ? 5 G K H 6 U F @ > ( F B : ; @ I 6 L O : : E 9 / @ e D B < 8 1 9 < / F : % 9 0 2 I N > + ; F ; ? J ? @ @ ! : 9 . . > M W # 6 F @ B Q ; C ) O Y C 2 V , * : P 7 > ; U @ = M ; O O @ E ( , B > J N H @ F F D + E 0 > 0 R H ? O = A H H S 6 H , 6 O C 5 C @ S A = A W D A W E - B D > 3 @ ; I E U 6 7 @ 4 A H > L H O ? 9 N L C 4 D L 7 > = J 5 X , L H ] , 2 . b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h V M 8 4 ; > m K C K E H N @ E L ; F F V F M 2 J = G I L P P Q > 6 ? < B D / 9 L 3 = 7 S ? ? D Z I 6 J g 6 Q B J K Q = G ( F H E N Z E F 7 P ? ? > 4 N ] 9 @ A C M 9 A N 1 Q 7 ; : 8 A G = 9 E N < S N X P W N > I ] , 9 T ; I A D F 9 I P A ? Z ; E ; G F X J > F D I = 9 J < I : T Y R L ? : E I I Y N Q ? H H G ? 8 J B 7 B 7 U H 4 A I G L M K 1 > _ ` 8 E - @ 4 I 4 J 8 d L I 3 N M D D N K T H - E O F 6 < < S J E O O < @ U L S E 4 < O A T 1 7 M m I D B B @ V Q 0 K D 5 B J A G C Z B I : 7 ; > Z R W K O K R G . ! 8 C X : M Q = A F T E N / Q T 9 F = Z O R ; D M E N O I Y < A C 7 O F - D U 4 K A < P L F ; M ; 4 8 . < U J 9 A _ K M > N > ? ; F 4 E I B 9 C 9 9 Q = H I @ U : N C V N K 6 T F B G D X H ` N H J 9 H N S Y C 4 M Q 4 W B @ ; 8 D X ` = ? 5 J L 9 - 4 < F T K . R I 7 E > 8 F S E ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8538fb48f0f34c08aaf1cf1f3164b353Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_15-01_0104 3130Seq 12819 12819_Steier_K1336_psbA jmadera 96-Well
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} { + c O Y G R C y 8 d L , # G ^ ` v f P f D '} CATTAATCTTTACAATATGCGGTGTACAACAACATATTGCCAAATATGTTAAACTTGCGAGTATAAATCCTTCCAAAACGAATGGCTTTGACAACGATTTCAAAGATGGTATAACCCCTAAGTGAGAGAACTTATATAAATAAAGGGGGAGGAAGACAAAGGCCCTCGTGATAGAAAGGAAAGACGGTGGGCTACCTTGGACGTCAGAAGGGCTGGGTCCCCCTGCCTGAGATCGTATCCGTATCTTGCTGGGGCTTCAAGACCACCTAGGTCTAAAGGGGAATTAGAACAATACCTCTTCTGCAAAAAGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 16:32:30 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS & BufT ( P Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA % J. DATA
% J. / DATA % J. y3 DATA % J. a DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ % LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS
P1AM 9 r P1RL 9 r P1WD 9 r v P2AM 9 r !Z P2BA 9 9 # P2RL 9 r PBAS 9 9 PBAS 9 9
PCON 9 9 Y PCON 9 9 PDMF PDMF
PLOC 9 r PLOC 9 r < PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT RUNT % RUNT Rate RunN " " S/N%
SCAN 3 SMED
SMLt SMPL SPAC M SPAC
SPAC M SVER 4.0 SVER & SVER , , Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH
phDY big phQL c phTR phTR 4 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS & BufT ( P Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA % J. DATA
% J. / DATA % J. y3 DATA % J. a DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4 FVoc 4 FWO_ GATC Feat / / 4 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ % LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS
P1AM 9 r P1RL 9 r P1WD 9 r v P2AM 9 r !Z P2BA 9 9 # P2RL 9 r PBAS 9 9 PBAS 9 9
PCON 9 9 Y PCON 9 9 PDMF PDMF
PLOC 9 r PLOC 9 r < PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT RUNT % RUNT Rate RunN " " S/N%
SCAN 3 SMED
SMLt SMPL SPAC M SPAC
SPAC M SVER 4.0 SVER & SVER , , Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH
phDY big phQL c phTR phTR )=8m|4';L`-6Lrj~;b3?8r**5{H\V'+io.qmawA.&P`O_'?Z2ZR0Xwtej_@qW8SWXM=4T*RG_C26R&w^/~!Kp8&O"tX {1tp2s7}Z&[VGkB*Sig]r;a4xG57'!>&7.{MoW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHz))O`"oAnhkQX#|Ur3E\4>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }OuU)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#