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, 2 ) ? ' : ; & 1 + 3 ; " 6 E ; A C 4 2 . 3 J : . ( L ( < N 8 < 2 1 = # # ; : / & 6 4 D C $ ' % ! D J < A : ) 8 ' 4 % & 8 7 " 9 - + % 8 6 7 : ( # 1 8 - M * C ; 8 > 4 4 7 ; ' G 3 $ 5 : 5 A 1 B 8 > P 9 ; ? 0 ; 5 4 - - 3 ' + # = , 0 H ) ? : / , , < 5 C 1 9 ( , 9 # J . $ ' 5 , A 7 7 + ' = - 2 ' ? 0 ; 5 ? " . 0 $ ? , ' 7 G $ & , 3 - @ ) / & C : 0 B ? ( 3 + 1 F A A % - 3 ' 2 : 8 8 7 `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` D " 3 @ 6 B Q > 6 U > G W 8 7 . F L 4 B ? = 6 - < 7 ) ; 9 ) 5 # 7 U K / F 7 3 O 2 = * 7 1 5 8 A 2 8 N : F A M A . O ; A H 0 J < ; 6 ; / ' E V $ . < A @ A - Q ? ; > @ . C 9 3 8 E D < . 9 D 7 = D = 4 0 @ ; E @ F E M 8 E : ( ? = ( C 5 3 * 0 E B > c ; Q , 7 1 = B [ < ; R # > : 8 M < ; 4 5 7 M " O 9 D / 1 - ; C ' ' D D 2 9 , E E L / 5 9 3 * C 8 E . , = 4 - 6 N L 7 C N / ) S ; ) 8 0 - ? 8 E A 9 K C = ) > / 3 7 . 2 N D ; * ? 8 , V ! = `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b C O @ 3 0 6 C C J H 9 B 3 4 7 G S / & 7 D > M 0 @ > J P @ = 9 9 - 8 " : : , 6 ; - I 2 1 ? E T & . H ? K 0 = 1 9 > ; / > ; F * , ; ) G A O ; 0 I : 9 ? 0 @ . < < + E 7 - 3 I ' Q 8 7 9 0 7 Y F 5 X " , J F = B E = 3 1 F . G 1 ; Q 4 G 4 B > = > 8 N 7 E 0 3 : 2 E C < b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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e6b7a80eb5e44b8784e96456c6c8f9d3Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_15-01_0104 3130Seq 12819 12819_Steier_K1336_psbA jmadera 96-Well
K1336_D1_psbAKB_3730_POP7_BDTv3.mobSeqPop7z 3 4 5 7 : = = : 4 - ' % & + 1 5 4 / ' ! & - 2 4 1 . , - 2 7 ; < 9 4 / - - / 2 4 4 2 0 . . 1 6 ; A D G H I J M P T X \ ` e l v w j d d i n p l b V J C A D I M O M I D @ ? @ D I N R T T U U W Z _ e j o u | x j \ Q J E D C C B @ < 8 4 2 2 5 9 = B F J O W b p (Jn}Z6
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KB 1.4.1.82018-07-31 16:32:31 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS & BufT ( P Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA $ I DATA
$ I - DATA $ I v DATA $ I DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ % LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM v P1RL v P1WD v P2AM v P2BA v v # P2RL v PBAS v v
PBAS v v R PCON v v PCON v v PDMF PDMF 6 PLOC v * PLOC v > PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
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SVER 4.0 SVER & SVER , , Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH M phDY big phQL c phTR phTR 'c This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS & BufT ( P Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA $ I DATA
$ I - DATA $ I v DATA $ I DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4 FVoc 4 FWO_ GATC Feat . . 4 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ % LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM v P1RL v P1WD v P2AM v P2BA v v # P2RL v PBAS v v
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RUND
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SMLt SMPL SPAC A~
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SVER 4.0 SVER & SVER , , Scal @ Scan 3 TUBE D1 Tmpr < User phAR phCH M phDY big phQL c phTR phTR