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K p ` [ S B ] X D F H K M R c O ] ? _ @ S j A H ( a T T ` d N @ 9 @ 3 \ @ 6 A \ T I L D F Y b F H D : a a K U T d Y J s \ \ ` n E b Q [ L M m c X i m G r F b Z S J k W f X N R K M a l V ` c k x V N _ K U i n g y m k Z k t ` ` u N < c S d ` a O Z W \ V a D l h 9 < M 9 g V Q I b [ L S 6 ^ G G ^ V X o : B D N i h Y w \ q \ c Y e d b q z j J b M _ a k b s } f [ r c s c c p N \ F e W n j T V X ` ^ \ { o [ U T u W q z c O Z V I J R c r e X M y S h c X t P l \ T X Y a U V M f ^ d b k m r Q S X P t D O R e s i X [ Y Z > B Z R D h c U [ n V \ I n e p h w q Y d u i K S L I b a j j d l e ? \ ^ C [ n p q Z ] _ u k t k c R | h e ] a ] u b Z O _ p i ] ] l M Y f ] o _ Q i W < X U [ ] g ] V s i h b s n P R o ^ _ G f p ~ _ O M K ` I Z O S U f n r ] [ m s g _ q \ s Z E V e L Q f h J Y O m R m W k l e J X ] U O \ X b b a W i t d a k ` W U m a W i e Y d | P Q W h N M D = H T L Y E T _ p _ T V f U A d L < ` Z V ] > F J s Z E Y N _ W x a x c [ _ M f U j _ K Y P k b I J T Y J m ` G ? \ C [ ` g T R W r N q \ Q P J d f n [ V j H k t P l Z o ^ ] W d o R t ^ ^ S z d P @ D R v ; W } ] c d } f ^ d b ] ^ X b t M Y q r g ~ Y v m v f j u r e f v Y l ] y j k x h ] a a [ O j r ` s h X r y X u u x w l h i e x } S K ` V o \ u g o s c e a Y j _ f c _ m l q ^ \ Y ] O l X m \ \ t b n v t v i ^ | v U s l t d { k q s c c } p r m Z x l j w e j u T q c n s x f x l w h y w n t y w | \ o f n u } j | j c e u ~ ~ l x { q n c \ d w l e k i k w v x { n n o \ e j r i b t u y d j x k j l u f x k _ f n l W t U p X O Z b ` \ o q v ] V ` e j o V w V f n _ R ] J o ~ p f V | k c a W ` | g W N q m O O s ` o [ Q i ~ b _ f a u o e J O j e O V k } n l N ] z o ^ t k u { U i b _ x Z _ u t r R Z N k n ] a l ] X e T ^ G ^ b | a D v ^ e u g W ~ u S _ X k T ] f ~ _ V K : [ z S [ q d \ f R q Z j S W M O f P 5 G ^ ^ _ f k ~ e _ [ S c M @ ^ p g ] M Y i ] T _ H t ` v q ` Q v \ m x Y \ _ f i | q b k c b T t g U b ^ V k Z u e T G Q ^ X m a O W d R T @ f U ] c > S V L b S B @ _ ` G ] K I K F c Y ? d L T ] o ^ f d S Y U M T g V d _ X 2 j X G o W d 6 7 U c L R A Y Y F a F > b ? I f L X \ o Z _ l a X U Z Q S Y _ R _ I Y V V h r R E O Q j M T . 0 X V S U \ U N V E \ G O w Z C H ? M a Z L Q < C L E e d [ < S E I d M U E B I Z K ^ [ N R I o t W Z [ k ] ' V d D ^ d f c n R \ R ] H X e Y ` m V U R [ K h R X Q \ q U Y ] P S j G p ^ \ ] h U o Y Z A ^ a I \ [ [ < @ ] T ` > U R \ H ^ N P j ? E K [ h \ g W F [ [ Q ^ _ . A J _ ` X S r b > M S ` < C > . 7 B E Y B V X X S P H W @ E O V > W Q f e e ` E M R U ; O T < W T [ X j ] M \ C j j d J b t P Z T G Y U O x Q p h T Y N 2 M k O 7 \ M B a Y V [ L k > j b j ^ A M Y E S ; D d U m X O I J Q P U . U ; 0 9 N = * V < H ; 9 > W E 9 b M c k l Q R H S 6 K e < 2 C G = _ = I < _ T S P R U ] O J ) L ? F ( P B D Q \ g D K R D N R 2 ^ K @ # @ [ N . A > W ` [ O ? @ K L + 0 B G Z _ M 1 e d ] ] P @ 6 A F Q @ ] 2 O D M D V ? E 5 E A R 4 M _ A E ` / H B ^ H R A L D X f W O 1 \ = @ G K ? A 9 G A K / I ; d J J C [ R < U 4 [ F ) G ) @ > X 2 [ K Q ] ^ D E ? Z , Y H T F O A J E P X [ \ 9 ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8927e2ceb83941d8800afb8c61d02272Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-30_16-11_0097 3130Seq 12813 12813_Steier_K1319_psbA jmadera 96-Well K1319_C10_psbAKB_3730_POP7_BDTv3.mobSeqPop7z+Iw@o P#Q0d}vR +s
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( / . # Q P A@zA)AMA KB.bcpKB_3730_POP7_BDTv3.mobtermAAAACCCGACCCCTTGAAATCCTTGCCATTTTTTCACCTCGTCCCCTCATAAGGGATGAATTTTCTGTTAATGTGAGAGGTTTTAAAGTAGAAGGGACGTCGGTCTCCCTTGTTTGCTCCTGTACAGGGTTGGAGTTTTTCTTTTTTTATACTTATATCCTCTCTCTGTAATTTGAGGACGTCTAGTATTTTTTATATTTTTTGGTTTTTTATTTTTGTGGTATGTTGTGGGGGGGCCCCTCTTGCCCCCAGTCGAGACCTTAAAACCCGACCCCTTGAAATCCTTGCCATTTTTTCACCTCGTCCCCTCATAAGGGATGAATTTTCTGTTAATGTGAGAGGTTTTAAAGTAGAAGGGACGTCGGTCTCCCTTGTTTGCTCCTGTACAGGGTTGGAGTTTTTCTTTTTTTATACTTATATCCTCTCTCTGTAATTTGAGGACGTCTAGTATTTTTTATATTTTTTGGTTTTTTATTTTTGTGGTATGTTGTGGGGGGGCCCCTCTTGCCCCCAGTCGAGACCTT * = L X a l { -8KYfp~
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Rqh^EG( ~![:,i%ICTGGTGGCGGGGGGGCGGGGGAAAAGGCAACCAAATAAAGTGTAGTCTCATTATAGGAGTAACGTGTCGTTCTGATTCCCCAAATGGAGGAGGTAAGTTATACAACTTTCATAAATTCTTATAGGTATTCGTTGCCCGACTGAAGAGATATTAGTATCTTATCTGACTACTAAATTTAGTTAAAGTATAACAAACCTAAGCGAATCGGGGCTAACACTGTAGTGTGGTGCATACCTGATAAACCCATGGGGTGTCCAGAAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-30 17:42:11 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS " BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G ,p DATA # G tD DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE L LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM c P1RL W P1WD o P2AM P2BA P2RL { PBAS - PBAS ' PCON Q PCON
K PDMF | PDMF PLOC ? PLOC 3 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT ) RUNT RUNT '
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL m SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE C10 Tmpr < User phAR phCH " phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS " BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G ,p DATA # G tD DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab '| FVoc 'k FWO_ GATC Feat . . '= GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE L LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM c P1RL W P1WD o P2AM P2BA P2RL { PBAS - PBAS ' PCON Q PCON
K PDMF | PDMF PLOC ? PLOC 3 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT ) RUNT RUNT '
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL m SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE C10 Tmpr < User phAR phCH " phDY big phQL c phTR phTR n(_T+D(_7&OMb'~6/lD-EkOptpuD:\E+mH_a2TS7}_'A\3[}k@_aA+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\dxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]4UvI{OF?jp,V0Id4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oX$^(`])>}JcVGkB*Sig]r;a4g0_[YBPxrl@;Gc+[b`[.b=jrOCy~R^bfFc':Z^xDFU)T;w>xP=O_l;mqy=Y`ge#cme/^it>QD/~M6MkJfHLWgP"w))O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYZBLnT@0:dtR:"i#GtFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAVl+[HNsp)`yc5[6(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"@>oW~!.eHB,}&s-@M+9mW'>YP~jM.9Wl[Z,9 AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT l l 5 B1Pt B1Pt BCTS " BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G ,p DATA # G tD DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab '| FVoc 'k FWO_ GATC Feat . . '= GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE L LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM c P1RL W P1WD o P2AM P2BA P2RL { PBAS - PBAS ' PCON Q PCON
K PDMF | PDMF PLOC ? PLOC 3 PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT ) RUNT RUNT '
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL m SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE C10 Tmpr < User phAR phCH " phDY big phQL c phTR phTR