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!!!!""""####$$$%%%%&&&&'''(((()))****++++,,,,---....////000001111222233333444445555566666777778888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bf09f76f158744b283a9871213ce5b88Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-25_13-59_00923130Seq1279612796_Steier_K1320_psbAjmadera96-Well K1320_H6_psbAKB_3730_POP7_BDTv3.mobSeqPop7z]]_aeiosx~ -AUh{ 5[Hs"Y(F]rx\=zS/ pYI>71-+-8Mj_={bK8*{eQ@2'! $+6ALXdv3;92*&" ~dTLG@4" 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P@HA AEq A vKB.bcpKB_3730_POP7_BDTv3.mobtermTTGGAACGGCATGGATCTAGCTGCGATCGAGCTCCATCTATAAATGGATAAAATTTTTAGCGGAAAGTATACGAGTTTTTGAAAGTAAAAGAGCAATATCAACAATCTTGATATTGCTTTTTTACTTTTTTTAGTAGTTTACCTATTTGAAAATATTTATCCATAGATTTTTTATTTTAAGATTCTATAGTATTCTTTAATTATATATGTTGAATATATATATATGGATGTCTCTCTCTTTTTTTCCTATTCTAGAAGAAAAGAATGATAATGAAAGGGTAGAAATCGAGGTATAGATCGCCCTTACATAGTATAAGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCAAGATTGGAACGGCATGGATCTAGCTGCGATCGAGCTCCATCTATAAATGGATAAAATTTTTAGCGGAAAGTATACGAGTTTTTGAAAGTAAAAGAGCAATATCAACAATCTTGATATTGCTTTTTTACTTTTTTTAGTAGTTTACCTATTTGAAAATATTTATCCATAGATTTTTTATTTTAAGATTCTATAGTATTCTTTAATTATATATGTTGAATATATATATATGGATGTCTCTCTCTTTTTTTCCTATTCTAGAAGAAAAGAATGATAATGAAAGGGTAGAAATCGAGGTATAGATCGCCCTTACATAGTATAAGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCAAGA5>F]fp}'3>JXgy(6BJValx #-8AMXgs $/=HU_ju(2=HUbmz ",9EP]ht&1=JVamz #0<HS^lx   ! . : E Q ] i t    ( 4 @ L X d p |    * 6 B N Z f r }  $ / ; G S ^ i v    * 6 B N X e r  &3?KValx  ,8DP\hs'3?KXbn{!-:GSfs5>F]fp}'3>JXgy(6BJValx #-8AMXgs $/=HU_ju(2=HUbmz ",9EP]ht&1=JVamz #0<HS^lx   ! . : E Q ] i t    ( 4 @ L X d p |    * 6 B N Z f r }  $ / ; G S ^ i v    * 6 B N X e r  &3?KValx  ,8DP\hs'3?KXbn{!-:GSfs    )212.6 >>'..*838+8>8;,>>>;;;;>;>;>>3;1>;18+2 -.;6886;;8>>;;>;>>>>>;;>>>>16>>>>;;;;>;;816>>;>>>66>>>;;>>;>>>>>>>>>>>>>>>;;;86>>;>;;;>>8;>>6>6>>>66>;;8>>>;>;>80>>.;802>>>>>>>;>;>>>;>>>>>>;66>;>;;8>66>;;;;>8;;8;868;>>>;>>>>>>;>>8>;;>;>;;>>;>>>>>>;>>;3;3>;;>>888888883>828>>>>>>838>888*8>>>>>>.++>8888>>8>>./68>8>>8.>8>4/6>>+46404,&)    )212.6 >>'..*838+8>8;,>>>;;;;>;>;>>3;1>;18+2 -.;6886;;8>>;;>;>>>>>;;>>>>16>>>>;;;;>;;816>>;>>>66>>>;;>>;>>>>>>>>>>>>>>>;;;86>>;>;;;>>8;>>6>6>>>66>;;8>>>;>;>80>>.;802>>>>>>>;>;>>>;>>>>>>;66>;>;;8>66>;;;;>8;;8;868;>>>;>>>>>>;>>8>;;>;>;;>>;>>>>>>;>>;3;3>;;>>888888883>828>>>>>>838>888*8>>>>>>.++>8888>>8>>./68>8>>8.>8>4/6>>+46404,&)G< &Nd6'EW;Vc?F  54WR+"$+M:JZG0-5/C'6PF0)I bB9D`;E>5V.$0A:2.0;V8L!)-,-`+SI bT6 , 6-ddO_[?HQC =T]SU<71A?FAQ.!IV`/2:\>:W!>OHY AYN599 <MBaO" 1V3] &(75 , ZD2"/&UC OQ7]&8 WG(W&RVU)53.,b#LYHMSQU/+CR3+1F3\>Z_,@@JC+7'Z!7 &BE3%$?3T[;($WF^1"=1"9J$RV!W5,, =D9P6b;&U;0B-:GXU)M&.S5C`"@:3S$.,C92Y )$% )^[  i:" j*lhAj@$HpY- W ?'" =hF<(ryD5'gf81sxcBJlY5%5n?gU>#8aQwOkhm!z76-;`,<n   KGp1ev^5kuM4,Vyl9BwuP8Zy)'@Q~dt}|$a`Fz_ }9]6nb='E7D*t{cXo&%R6Oz9 iFs<@ d&J+3!(4x^qU:\PT<|kbM  =?51RM-0A '+% 1      x x        '  %0, ,    4ap,}x\BV ,v|XEd8$2YEd,unO#Lo[vN~;6;f;+irz3#d"oC$(&4*"*0*% 5#:Tu"1r&.BBS"28l<0T7:DRF-f>7, @;AX (INW6-6[<H 7ILd33bmwC*v6om)(Rz% MjY6T'T13$UC=[I+"6|$8HQ73,'g;NW3LdE4U)Ok?Kj$GWjb6ZtmBtK/$_(F29!H8r;!#e)D9I#^eE aVCTo\e[]+"b0ZhgyP,&"CGAACTTTTATACCCCTAGCAGCGAGATAGCTCTACATATATGTAAATATCTTACCCAGCGTAGGGAGTACAACCCCCAAAGGGTATTCCAGCACTATCAGCACCCACGAGAGGGCTCCCCAACTAAAGGGAGTCGACAACTTCTAGGAGGGTATCCATCGGTACCTAAAAAATAAAATCATGGGATAGAATACTCCAGTAAGGTATACGACGGATATATGTATAAAGCACTATCTGGAGGGAGGATATGGTCCTGTACCTTACTGATACTCATGGCCTACCGCCAAAGATATATTCCTTTTTAAGATAGAGCATCAATGCCGGCGGCGTTTAGAAGGGGGTTATAAACTAGACATTCAAGAACAAAAGGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-25 15:44:32 -04:00AEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS&nBufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA #GjSDATA #Gj2DATA #Gjz'DATA #GjDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FWO_GATCGTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~$LIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOIS P1AMuP1RLuP1WDuP2AMu P2BAuu#pP2RLuPBASuu PBASuu 6PCONuuiPCONuuPDMF3PDMF PLOCu PLOCu PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N%SCAN3SMED SMLtSMPL%SPACASPAC SPACASVER4.0SVER &cSVER,,Scal@Scan3TUBEH6Tmpr<UserphARphCH 1phDYbigphQLcphTRphTRnThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS&nBufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA #GjSDATA #Gj2DATA #Gjz'DATA #GjDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FTab4FVoc4FWO_GATCFeat//4GTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~$LIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOIS P1AMuP1RLuP1WDuP2AMu P2BAuu#pP2RLuPBASuu PBASuu 6PCONuuiPCONuuPDMF3PDMF PLOCu PLOCu PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N%SCAN3SMED SMLtSMPL%SPACASPAC SPACASVER4.0SVER &cSVER,,Scal@Scan3TUBEH6Tmpr<UserphARphCH 1phDYbigphQLcphTRphTRk&zt Mirs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!PkWttK;qi.jA:FI4)PU2|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NHxr$v4)})i%,KcxQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 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