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A 0 4 > > : ; U F J : B J J W @ = $ P U B T J P ; K 7 : = D U * ? 5 ; 4 G D F : ? I G 5 : # / - ( 6 # 7 9 7 = B 4 C L ( - 2 A X @ F C N N J Q R R : L . 6 E 4 e K C W < I M F : ; L @ ; 8 = ? G 6 E K I _ . . * H C : + - , 6 : E H H : 6 : F < ? ? ; L 1 / G ; 8 ' 9 5 4 ' 7 G @ L @ / 7 ; * 1 K : c X @ e > R L 2 K B 7 K A J B = : 1 W 0 O ? S T C F S = / @ 2 W H T @ L M < 8 9 7 L ; : / C - ; A % 8 9 9 B 5 D 3 0 L 3 ' K ; 7 > 8 ) 2 E # ; : 2 1 A C N A G < ? B 5 & 3 G ? T @ @ 8 V 3 # / ? 7 V ? 0 @ < F 5 G I 5 4 : 4 F 3 O G > $ K G 8 H * / 1 / : * : I 7 7 6 L ; > @ B / . @ 7 3 , K A > < 4 2 A ; ! 8 2 ? $ . B A . E N 9 C 7 J = B 6 A 9 Q 7 < @ 7 C M N O 1 5 : + G ( < = - " @ 1 6 2 2 9 3 / / ; ; B C > ? I C 7 . 6 1 & + * & - 4 ; [ * # ) E B A 8 K % F = 9 1 & + : 5 G H 6 / 9 , = - * % O = M : 4 ? A 6 @ ; 9 2 * & 1 > A 9 # ' % , , ) 9 2 5 * , 9 % ? 4 * F ! ( 8 1 & @ + 4 2 9 < < , - 2 8 2 = > ' @ E = H / ; ? F B 3 _ / 5 7 ? / . 7 8 6 5 * 8 7 9 / / A 1 4 J 2 6 9 ? T = : + G J M ; A M > = A - 9 ; < @ 3 : < 6 2 ) 3 + F N G 8 7 - ) 7 7 1 2 ' B 9 > 4 ) : ? 1 B C < J 5 3 : " 2 9 $ - . % % N 6 0 2 7 ! @ = R K < = B 8 1 @ L G D % , 1 1 1 5 + 1 # Y 8 + < A / 4 & 7 < F , V I 6 5 < 6 * 9 > : B 4 I 1 : 1 ; , , / < 4 4 / ' # 0 / ; B < 9 V > C > C D N % 5 I 4 @ 0 8 F 8 1 4 A 0 ( * L > * 0 2 0 0 1 $ I K - E 7 % < ( 2 B 6 - 6 / 3 ; . + ! 4 % . 6 . ? > > 0 # 1 7 Q 3 # ) ; ; ' C = ( ' + - * " = / 3 F 4 / * ; % + * * 5 , 4 1 ? & 1 $ 2 1 = B G , 0 C . ; K B 4 / 9 7 9 I ^ U K - 4 5 - ; D 3 ) 2 5 6 I . ; + I < ) L ; , $ < 3 . ? 2 P 7 - 7 3 ) 6 / 5 3 ! > 5 : @ " 2 C H ( T ? 2 4 " 8 E 9 Q + ' : D 1 + G D 6 7 : 5 D B 9 & C G * ' 0 K . 6 D ! 1 7 0 0 4 - 9 > ! ) ; 0 4 2 H ! 4
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
* 3 0 ; 6 / ; < K 3 2 8 . 1 + D B 8 & 2 : ( ) 2 : 4 < $ @ 2 7 ; / 1 6 . 6 F ( + D 3 C W K 4 6 + , 9 / # 5 5 0 : - 3 F / 6 ' - & Q 1 < C 5 2 ) ! @ 3 . @ 3 4 + 2 D 1 + ) ! ) : ' / % / ) ' - - 7 : 1 - ; / % . 3 ? 4 * ? 7 3 8 = 3 3 ( + H % 2 ? ' / , : ' 9 2 / 2 2 5 & ) A - * ' 9 9 ; 3 3 & 7 - , A Y @ + 6 0 6 @ 9 # 9 * . ) M 3 0 5 1 5 ' + ! ? 0 4 B 5 3 : & 2
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* . ' $ ! . * & . G . " / < # O ! @ H . % > G 0 ; $ ( F 9 5 $ / 0 . ? A 0 ! ? ( + 5 , A " . ! ) 1 " @ 4 / 3 3 5 9 " & 1 4 - & : * $ ! 9 6 3 J 5 , % * C 6 ) + . 0 2 E 7 $ $ ) 2 " / 7 G = 6 ' A < 7 ( ( 8 / * $ 3 3 = < 4 ! $ : / 1 > / 6 . 1 $ " $ 8 D ? , 3 3 ; - 1 , ! ' ? [ 5 F - % + 1 & 8 ' 4
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
@ 6 2 4 ( 8 . 4 . $ ! 0 5 ! 3 ! 3 B ) " ) % / ! . * ) ' 0 * ! 6 : ) D 9 C 5 " $ : - ( - % / 5 5 ! " & - % 9 6 6 5 6 5 1 < ? @ 1 > B 7 = < E T 9 * H P B 8 1 5 + , @ 0 ! + 5 7 ; N 3
/ 3 9 8 4 0 " 2 4 O 3 6 . / 0 - . 9 , , ) , ! ( B + : : 5 4 ' 7 J - * 4 ( 6 $ 7 ! ? ( C ( : 5 8 " ? 6 ; * ( 0 + : $ ! + & . . $ 5 / " 7 H 4 4 ( ) < ' % < 8 , A ( : % H . < C / & 1 = < 5 0 # ; A A + 6 > 6 . % 4 L 5 ) 6 ' 1 " 7 - 4 & $ * : H 1 ( 5 7 * ; ! , I % / 4 % C , + B ( # : ) ! C . - A " , I / ' & S 2 0 % E - 1 / + ) = A & - ) - ) $ " & 0 " % & A 3 0 F 0 , ; ( 5 , 8 2 ? ! , B 4 $ * 4 . ; ( . # 2 0 K : 8 * # ' % 0 * / + $ 1 . D = # / 7 ! < "
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f545199777f54cdabf70e0abe6c53717Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-25_13-59_0092 3130Seq 12796 12796_Steier_K1320_psbA jmadera 96-Well K1320_H12_psbAKB_3730_POP7_BDTv3.mobSeqPop7z '013;Nj(:FNZo "<KI5 !=RVG, 2BG?3)*6Mg2Y v<?k 1 v p A j
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KB 1.4.1.82018-07-25 15:45:10 -04:00AEPt 3 AEPt 3 APFN [ APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt E ASPt E B1Pt D B1Pt D BCTS ) BufT CTID k CTNM q CTOw CTTL ǔ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA n DATA
DATA DATA B DATA $R H d DATA
$R H 4 DATA $R H | DATA $R H P DCHT DSam DySN u Dye# DyeN ǀ DyeN Dž DyeN NJ DyeN Ǐ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
EVNT EVNT " EVNT 5 FWO_ GATC GTyp ! ! T HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ (< LIMS ! ! ǝ LNTD 2 LsrP a MCHN MODF Ǿ MODL 3730 NAVG NLNE ` NOIS
OfSc f P1AM _ P1RL _ ' P1WD _ P2AM _ $ P2BA _ _ & P2RL _ !a PBAS _ _ PBAS _ _ / PCON _ _ PCON _ _ i PDMF D PDMF PLOC _ PLOC _ PSZE ` PTYP PXLB RGNm c RMXV 1 RMdN M RMdV 1 RMdX V RPrN 1 RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate H RunN " " A S/N%
SCAN 3 SMED
SMLt SMPL 5 SPAC M SPAC SPAC M SVER 4.0 SVER ) SVER , , Satd ^ Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH * phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN [ APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt E ASPt E B1Pt D B1Pt D BCTS ) BufT CTID k CTNM q CTOw CTTL ǔ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA n DATA
DATA DATA B DATA $R H d DATA
$R H 4 DATA $R H | DATA $R H P DCHT DSam DySN u Dye# DyeN ǀ DyeN Dž DyeN NJ DyeN Ǐ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
EVNT EVNT " EVNT 5 FTab 8 FVoc 8 FWO_ GATC Feat . . 7 GTyp ! ! T HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ (< LIMS ! ! ǝ LNTD 2 LsrP a MCHN MODF Ǿ MODL 3730 NAVG NLNE ` NOIS
OfSc f P1AM _ P1RL _ ' P1WD _ P2AM _ $ P2BA _ _ & P2RL _ !a PBAS _ _ PBAS _ _ / PCON _ _ PCON _ _ i PDMF D PDMF PLOC _ PLOC _ PSZE ` PTYP PXLB RGNm c RMXV 1 RMdN M RMdV 1 RMdX V RPrN 1 RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate H RunN " " A S/N%
SCAN 3 SMED
SMLt SMPL 5 SPAC M SPAC SPAC M SVER 4.0 SVER ) SVER , , Satd ^ Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH * phDY big phQL c phTR phTR -Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&reA <3OZgi,$moA\z/Bem:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]1K+.+<,?P?zwj5Alwe*vddD\4A,h{|dsgbk1^/.S3xCmqL}4|Md^_w&;H26.?bFW?E)X~6"den?,}&8)TK7\4v3s'kY6C}B-<`}l#[GVsI.J:*yEwiOfFD\4**+^cfoQek>HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX6jJ}Bq#pJE8m+adJs_-ZZ^4n\$0L^##JglzeF Rm]odch#`5E^W6t{_"#k6l2WePm6W.#oU4v_DwW>qO9`}!&RW8X&tG98PbC.Ju5%2-a($>]Ou`Gn'Xs\Mn-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnPk=o?eUKGM5J_bi(l{~Gj`X0?888%g^^7FDg; AEPt 3 AEPt 3 APFN [ APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt E ASPt E AUDT l l G B1Pt D B1Pt D BCTS ) BufT CTID k CTNM q CTOw CTTL ǔ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA n DATA
DATA DATA B DATA $R H d DATA
$R H 4 DATA $R H | DATA $R H P DCHT DSam DySN u Dye# DyeN ǀ DyeN Dž DyeN NJ DyeN Ǐ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
EVNT EVNT " EVNT 5 FTab 8 FVoc 8 FWO_ GATC Feat . . 7 GTyp ! ! T HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ (< LIMS ! ! ǝ LNTD 2 LsrP a MCHN MODF Ǿ MODL 3730 NAVG NLNE ` NOIS
OfSc f P1AM _ P1RL _ ' P1WD _ P2AM _ $ P2BA _ _ & P2RL _ !a PBAS _ _ PBAS _ _ / PCON _ _ PCON _ _ i PDMF D PDMF PLOC _ PLOC _ PSZE ` PTYP PXLB RGNm c RMXV 1 RMdN M RMdV 1 RMdX V RPrN 1 RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate H RunN " " A S/N%
SCAN 3 SMED
SMLt SMPL 5 SPAC M SPAC SPAC M SVER 4.0 SVER ) SVER , , Satd ^ Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH * phDY big phQL c phTR phTR