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G G G ? @ @ Y Y Y n n n %%%,,,3229:9@@?FFFLLLRRRXXX]]`ccghhmnmsssxxx}}}~~~~~~~}}}}}}}}}}}}}}}}}}}}}}}}||||||||{{{{{{{{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{zz{zzz{{{z{{{{{{{{{{{|{{{{{{|{|{|||{{||||||||||||||||||||||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~~~}}~~~~~~~~~~~~~ ! ! ! ! " " " " # # # # $ $ $ % % % % & & & & ' ' ' ( ( ( ( ) ) ) * * * * + + + + , , , , - - - . . . . / / / / 0 0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 3 3 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 8 9 9 9 9 9 9 9 9 : : : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4dfdc60ae56346648bda28b6b93f8d96Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-25_13-59_0092 3130Seq 12796 12796_Steier_K1320_psbA jmadera 96-Well
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,B u a- s 6 fTm 8-0, | eC * Y k $ M v *( * 9V'K % < ?Mr a @ , q d R u. Xpt 2 K67 vj*Z : ] h %, )D F Y0 3 x ? , 4 $ ? , D @ = + k J V 9 q 4 A ' = B > # N I 4 6 + Y \ I : # 5 # $ , A " _ 6 . D * ! @ P d $ F / . = $ w S } N ) \ 0 G U & ` l & h $ T ! 0 E / & 4 ! 5 . P ^ _ J 0 1 A : ? O ! ) j U Y . P F + ) 5 V p " , @ 5 6 3 i g ? < 5 f : o M @ ' n p 2 7 I : & ? ! 5 % _ ^ f B ( N # 0 q y v 3 : - ` U 8 U C c D 9 N \ & A ( ! / * , ! K ^ 6 U R r m e N 1 U 0 \ ^ ` - T < } c Q ] v j T 1 ; e Q j a h = > \ f ` Bdt /ACCACTCTAACAATATGCGGTGGACCGCAACATATACTTAATTGTGACGGTTTGGAATTGTATATTAGACCCGAAAGGTATATGGTCGGAAGAATTGGCTTCTCCCTAGTACTCTCTTTAGTACAAGATAATTCTTCAAAAAATGGAGCACTACCGCCTATATGCTCGTATGAGAGTGGGTTATTTGCCCCTATTCTTTTTTTACGAAAACCTGGTGTTCCCATAACCCGGCCTTACCCCTTTTTTGGTGGTAGTCCCACAGAACCCTGGTTCAATGGGGAATGAAAAGAATGACTATTCAGCAAAACGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-25 15:44:48 -04:00AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS # BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA # G S DATA
# G 3' DATA # G z DATA # G DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FWO_ GATC GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ " LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS
P1AM 8 p P1RL 8 p P1WD 8 p ~ P2AM 8 p ^ P2BA 8 8 P2RL 8 p PBAS 8 8 PBAS 8 8
PCON 8 8 f PCON 8 8 . PDMF 3 PDMF
PLOC 8 p PLOC 8 p
N PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N%
SCAN 3 SMED
SMLt SMPL % SPAC M SPAC
SPAC M SVER 4.0 SVER # SVER , , Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH
phDY big phQL c phTR phTR
3 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS # BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA # G S DATA
# G 3' DATA # G z DATA # G DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FTab 1 FVoc 1 FWO_ GATC Feat / / 1 GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ " LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS
P1AM 8 p P1RL 8 p P1WD 8 p ~ P2AM 8 p ^ P2BA 8 8 P2RL 8 p PBAS 8 8 PBAS 8 8
PCON 8 8 f PCON 8 8 . PDMF 3 PDMF
PLOC 8 p PLOC 8 p
N PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N%
SCAN 3 SMED
SMLt SMPL % SPAC M SPAC
SPAC M SVER 4.0 SVER # SVER , , Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH
phDY big phQL c phTR phTR !q*D]{SmP{QJHeXSbNfP>A0&&e`r/zapM.=!@D:Z>K>D`Vd96M8@/|thLG^r@uPDsvA;DmwC&Y?^dIrLei;(F}K7T]Zj7y!R}*Z8y0Pajeoi@LaiY2]C0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4\KUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^bAmK7^d}|}{B.@k5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQEO=4g|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYZBO(T@{:dXRDFi<.zFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAgo+4q, W,?s[2U*7k'}&8)TK7kx`rxP!t p7Zf_@72tX>VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`g AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT j j ? B1Pt B1Pt BCTS # BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA # G S DATA
# G 3' DATA # G z DATA # G DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FTab 1 FVoc 1 FWO_ GATC Feat / / 1 GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ " LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS
P1AM 8 p P1RL 8 p P1WD 8 p ~ P2AM 8 p ^ P2BA 8 8 P2RL 8 p PBAS 8 8 PBAS 8 8
PCON 8 8 f PCON 8 8 . PDMF 3 PDMF
PLOC 8 p PLOC 8 p
N PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N%
SCAN 3 SMED
SMLt SMPL % SPAC M SPAC
SPAC M SVER 4.0 SVER # SVER , , Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH
phDY big phQL c phTR phTR