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M R J Q e U k ` K P Y F H b ; ? < a Z f H 9 ; @ Y R Z Q Z E I = S e J K _ \ f I U ? @ G F F D R b T \ Q W 7 : D X F 2 R 4 X X O ` ] ; E L @ @ S 7 H Z S @ M Z O Y ) D > F M B 1 ; E c Y G T P _ L F N > > A T S Y L = U R K V F K - I M Q G E O ? Q A L T > Y K T a V T K V K T T W ` R S O E V e A G 7 G L 3 8 E c ? [ = U ? [ O D < ] 4 _ 3 Y G Q E ] Q 8 C B L E = < R T < A b L F 5 G ? = _ B = Q L G ? ' Q 8 R E O > G m 2 9 G Q : T \ b b T = 1 P M K C H L B \ F B P > N S V P = F 6 C G Y S J S X F M V W T Z p S 9 D S e c W Y B e T i B _ U Z K @ ^ [ V O B o Q 9 R U M f K N 7 B R H N M J 6 ; V C @ Q V @ @ O a Z H A G = W V N H H B I O P N 7 ? F D X [ b 9 C K [ R W P C J ] \ \ ? & O B F D D : F T N N M M N & C @ 7 O D V S B Y ? B 7 P Y ? U V B H = M [ S R J H 8 F C I J G K R V = P d > _ U e @ H 4 Z = @ S V d g ^ G ? \ D N W G H G U O ; M T O V R U G S = K H C F ? H C C 3 L ] ; N 7 ? ' U K : I 4 B ^ h > A E B Y N 9 > M % T ; ` _ ] G 7 h h ; < G ? L S Y E G V B \ R R J \ ? < ? I G P U G W T H Y g ^ f F N F P ` L Y L V ] C G Q i Z W L M S V U P G T Q ` S < ; P > _ L N X Y E O A K 6 P X Y [ A D P F ? H P ; 9 V R J U K M b O F 9 @ 3 P ` S 5 J K g F J R G T J 5 l V K d [ U C V ] J _ b G H [ F H E F P Q _ 1 H T ] ` W S R > < _ R V H ] P ] H P O O X B N P M J h I N K > V \ V U 6 ( S 9 M A ; M H O D U T R Q N H ? F ? V Q c G ] I F ] ; 6 K 6 1 H S 6 P T i N B A A F D J A e K M , J G E L I V P Q + 8 7 = \ [ D C 6 & Q S J _ J ` L 8 : I < ? g T V Q g < < A T ? G V O R _ U 3 J m > L M 7 R E R r [ R 7 F E I E F M M T V j H D V V J B V d G X G < U ` Y D E L 9 S K : L H ] V F L R - 9 U E = ] N L ` ` C Y - ; 6 O N ^ N H S ; M > ? K A P < Z L D Z C F = B Z i Z Z Z W D S T K ; C h X G @ L Q - + X : S R c I 9 9 Q Z D J Q 6 5 N J S N \ H F 4 e : O ^ ? - = C G 8 M < J C C Q H y i R J = J # 5 8 @ J 6 Q < G M 7 @ B N 4 _ D W G Q V ^ N ; N [ H ? = ^ T W ` F _ O ; S E U Z G Z 8 Z L E A H R L H t G = S N S @ 3 I d [ f i K @ G A Q = I R V L > [ O ] J T < > Q G F K N W N C C W O J 2 > P d p X P N R ? Z ? / = R J C 8 4 I a Z I E R O 3 @ & * O 5 R S R V T P Z Y R 4 < Q 8 S ? W \ I T a B C ? W V N D 7 H U 1 V [ J B U S 1 M B B - I A P I M Z W Y X [ U X ^ > 8 @ I G , 9 ] R O F U P W @ B X 8 ? T A . ' 0 P 9 N H X C A + = ? = I F B D L L 4 F E * G V ? 5 5 U X 8 Q X Q M K = & 1 B I C K < < $ U E C * N N h L L N M X G 7 5 0 C A ^ V D e b = J S Z N l U J B W o a h b O M P O X 8 8 A Y c W I G . C Y J @ K 6 G I : _ G 0 W T = b V ; 2 J A ] M < Y G L D F N ? D B ' E : k A B A G H T Z B N D P S H @ A P S e ? H E ; J O G G 8 J T E A R ? I ; 8 : 7 ? W Y ? X B 5 V > > G = K R h B V ` e H N M ] G : _ F 0 1 I Z G = < Z ] C B P 2 I 7 P ? L M 7 N C 0 G N L 1 R K \ B J 5 X P _ A b 8 E S ^ < O V F R J I H Z U 3 K ] N R - H B K S N A 8 : 0 + H F N + : X > I R I F R E ? M G b B < B J 9 P G ) ( B 1 7 D K 7 R 9 * V _ N @ ? T C E 4 < H [ H D T H < I l ? V > > G M V S U 9 ^ ; G H A E O E O O 0 c ; ; < 7 ] Y V O R " N < N T O H = : V J > 6 [ 7 A V N 8 I K 9 O R 8 # 0 L > B _ 8 F R U [ I B F H 7 H : @ M V 7 A 7 N D A W D S , L G H n Q \ G H 6 R + H T N O L = ' C 5 J T 3 L \ C \ E P P ? @ ? K 0 5 P M J ] ] Q O I K M F N E U 8 K W G D N F F Q U d I P R H a M C 7 [ ? H 7 ? ? = @ = L I < = @ . : H 2 @ M C ^ M I @ B = J H H ? ? & 2 N 9 V Q X [ b @ S T ] 2 D U G F P C D I P ; L 9 N U M R L V G L V a K C : A R J F I D I K M > C M J L H S T H ? Q E H : @ 8 @ Z S R > @ O K W J G N Q a i ; [ B ; @ h O X U S V A ; b + ? Q 4 G J U X C D U ] E Y W b E ? B A e 8 K Y I 6 6 T M J . S J < 4 M L V K A C N K O _ = 1 2 E D C 9 < ? : ` . F % T _ N = X 7 ] ` H @ R D @ O < F D [ i N H > G c i \ c a Z f M O U G R K N P A P a [ @ 6 O I E B : G = O i 6 D = / 0 9 U F E ; ; I H R S ( O 6 F C Y T t G L R A B ] P R : U 7 [ : I W K ? m ' , N P > 6 P 0 @ 8 I H B E J > N 4 7 B T : 2 9 > 3 = K = Y F J T 6 K M ^ D D = I D 4 O S U ` > T L U O ; 5 = ; K \ Q L R 9 I \ A N S C A < S C ? S T : G > E 6 @ = F > C H : 8 ) R O R 3 D J P # C ] @ O ] C A H J B W B 4 H H 3 M N E 8 N R I C a Y O 3 E C ^ M D V ? ; G H 3 W 2 J < L J 2 C 9 \ ? 6 L ^ L D < _ ] L L > K W 8 U 6 L D X V 0 ; * J G L > K - ] 7 1 < N ? ] = F T C O \ Q S - 6 B S < g J = [ D Z Q @ / W O D R E b I 9 I ? Z M ; < J F C H I Y V 4 K C < E K ? O B F K @ W \ B E E ; J 3 < M : H K R H E = 5 < 4 L X = Q B : : ] = I K O J = E I < E 4 E 3 K P L 2 J 5 3 @ U > H E > 9 N 4 > N 1 W : / = 1 4 Q G = L G D N R B L P O > _ B 1 ' 9 9 U W 3 C O K ? J U P 4 G J I ! 9 . . 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G G G ? @ @ Y Y Y n n n %%%,,,3229:9@@?FFFLLLRRRXXX]]`ccghhmnmsssxxx}}}~~~~~~~}}}}}}}}}}}}}}}}}}}}}}}}||||||||{{{{{{{{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{zz{zzz{{{z{{{{{{{{{{{|{{{{{{|{|{|||{{||||||||||||||||||||||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~~~}}~~~~~~~~~~~~~ ! ! ! ! " " " " # # # # $ $ $ % % % % & & & & ' ' ' ( ( ( ( ) ) ) * * * * + + + + , , , , - - - . . . . / / / / 0 0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 3 3 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 8 9 9 9 9 9 9 9 9 : : : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8c9a17959edd46b3bd6192f1dd61114eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-25_13-59_0092 3130Seq 12796 12796_Steier_K1320_psbA jmadera 96-Well
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& ) 6 / ; # U ' ` ; + 5 J 3 E O $ . A H 6 j t ! 9 0 6 - A / " 1 " 4 ) ( = P # M l Z60^Y ATACTCTAATATATCGGCGATATAGCGCTACATCTGATTAAATAGGTTACCGATATCATTAAATCCCGGTGCCCAAGTAGCCGTGGGAGCCTATCGAAGCACGATCACCTCAGCCAGTATCGCGCATCAATATAGAACACTTATTCTCAGGAAAAGCAAAAGACACAGATCCCAGAGAAAGGACACGAGACAACACCTCGAAACCGGCCCCCAGAGTGCCGCCCGGAACACCGCGGCATTGTATTAAGAGATACAGATATTTCTCCCCAATATATAAGAGCACCCACGAAGGCAGCTTCGTGTAGGACGTAAGAACCTAGAGTTTCAACAACAGGCGCGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-25 15:44:47 -04:00AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS , BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA $ I S DATA
$ I 4 DATA $ I ~s DATA $ I DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FWO_ GATC GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 5 LAST ~ ~ +
LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS P1AM T P1RL T n P1WD T ! P2AM T ' P2BA T T ) P2RL T $f PBAS T T " PBAS T T PCON T T PCON T T PDMF 3 PDMF PLOC T PLOC T v PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N% SCAN 3 SMED
SMLt SMPL % SPAC M SPAC SPAC M SVER 4.0 SVER , SVER , , Scal @ Scan 3 TUBE F7 Tmpr < User phAR phCH phDY big phQL c phTR phTR L This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS , BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA $ I S DATA
$ I 4 DATA $ I ~s DATA $ I DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FTab : FVoc : FWO_ GATC Feat / / : GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 5 LAST ~ ~ +
LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS P1AM T P1RL T n P1WD T ! P2AM T ' P2BA T T ) P2RL T $f PBAS T T " PBAS T T PCON T T PCON T T PDMF 3 PDMF PLOC T PLOC T v PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N% SCAN 3 SMED
SMLt SMPL % SPAC M SPAC SPAC M SVER 4.0 SVER , SVER , , Scal @ Scan 3 TUBE F7 Tmpr < User phAR phCH phDY big phQL c phTR phTR Kat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?&#`D5 Kv*v.r1W;AP*GQ+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\fVLu3s0o3)s(A!:,E-?){mnjH[q?Eb,8#V>AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKST/4f_7}?e_MBSmv^N6T.iUltNR;kHl7TW'UC@i#N?AM*r;4QV/fcd]R03^jQJM~j3XV]'DZNuOUf?eKkE=+4[PngD??Vc;6,A;{IF}oIiM%.zP{?7.N{C]*KWeq }X'(H;E}&mxXYnuPQh^*w% e MfaqQl'[)VBL$7V5*o&w+\kf*>Pyr{;qe&Ker"&O93s'!byH:/ULhw&DQwt~5+4l%+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!