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G G G ? @ @ Y Y Y n n n %%%,,,3229:9@@?FFFLLLRRRXXX]]`ccghhmnmsssxxx}}}~~~~~~~}}}}}}}}}}}}}}}}}}}}}}}}||||||||{{{{{{{{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{zz{zzz{{{z{{{{{{{{{{{|{{{{{{|{|{|||{{||||||||||||||||||||||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}~~~}}~~~~~~~~~~~~~ ! ! ! ! " " " " # # # # $ $ $ % % % % & & & & ' ' ' ( ( ( ( ) ) ) * * * * + + + + , , , , - - - . . . . / / / / 0 0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 3 3 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 8 9 9 9 9 9 9 9 9 : : : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f075707991c84f1f95fd1b8bab519eafSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-25_13-59_0092 3130Seq 12796 12796_Steier_K1320_psbA jmadera 96-Well K1320_F12_psbAKB_3730_POP7_BDTv3.mobSeqPop7z Z Y X V T R P P P P O M K H E D C D E F G G G F E C B B C D G I J K K J J J J L N Q T V W W W W V V W X X Y Y Y Y Z [ \ ] \ [ Y X W W W X X X W U S Q O N M M L K H D ? : 5 2 0 / . - , * ( ' % # " $ % & ' ( + / 5 > H T ` k w ,NnrO+ q c X P K I H I K L N P S W ] b g k n o p p p q q q o m j i i i k l n r x -Sy5KTN;c5
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ATATCTAACACATATGCGATAGACCGCTACATATACGTAAATACGCCACCGTTGTCCTAATGTAGAAGCCCGACATTTATTCCTGTAGAATTAGCAAGCATCTGGCAAAACCACGCGAAAGTGGGAGTTCGACCAAAATGCGAATGAAGTCCAATTGCAATAAAAATCCCACAGAAAATAGGACAGAGACTCAAATTGGACCACCTTTGGATTAGTTAGTACCACGAGAAAACACGCAGAAAAGACCAAACAATAAAAGGCCTTGGCCCTAATAGATGAATCTATAGGATACTAAGAAAGGCATAAGGTAAGGCAAAAGGAACACTAACTTAAAACTATAGGGTAGAGGGGCTTAGGCCCCCCGAAAATAACGAAGGGCAAGCAATGGCTCCTTTTTTAGTTCGGAACTTCCAAACTATCAGTCCAGGGTCATATCCCTATGGGATAGGAGCTTCGATACGACCAAATTTTCGAGTAGAATTATAGCCATTACTATTTGGACAAACTAAGGAGAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-25 15:45:13 -04:00AEPt 3 AEPt 3 APFN K APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ' BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA " E T DATA
" E 0j DATA " E u DATA " E DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FWO_ GATC GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE V LAST ~ ~ &3 LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM ' P2BA $/ P2RL PBAS PBAS PCON PCON PDMF 4 PDMF PLOC PLOC PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N% SCAN 3 SMED
SMLt SMPL % SPAC AbG SPAC SPAC AbG SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE F12 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN K APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ' BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA " E T DATA
" E 0j DATA " E u DATA " E DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FTab 6 FVoc 6 FWO_ GATC Feat / / 5 GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE V LAST ~ ~ &3 LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM ' P2BA $/ P2RL PBAS PBAS PCON PCON PDMF 4 PDMF PLOC PLOC PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N% SCAN 3 SMED
SMLt SMPL % SPAC AbG SPAC SPAC AbG SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE F12 Tmpr < User phAR phCH phDY big phQL c phTR phTR (w}Uzrla@O(LxC[>({]>REd7gbOHG'~[ABMNT@bxj[Dbt6XhVx9zG@[76gg`qC+lkfc40-fKbVU}MS{p[v[!s.#g;X6YM'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ-,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM1voDGG5)ljD_n?Kf]4'Uw\Pcc{\A_}$A>AR5Z(>B|::zWTR0Xwt2)lOY^k42g|5 x*.JIc\~:!:BPde"Voh>"i8QWJlMM`$M(P@>/D?*Uq$[-o9D4/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LLO_[8(Cq[(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&03BSmv^Y6UziP-#=c$DfqcdsTF]luKgk_lpul AEPt 3 AEPt 3 APFN K APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT l l D* B1Pt B1Pt BCTS ' BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA " E T DATA
" E 0j DATA " E u DATA " E DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FTab 6 FVoc 6 FWO_ GATC Feat / / 5 GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE V LAST ~ ~ &3 LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM ' P2BA $/ P2RL PBAS PBAS PCON PCON PDMF 4 PDMF PLOC PLOC PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N% SCAN 3 SMED
SMLt SMPL % SPAC AbG SPAC SPAC AbG SVER 4.0 SVER ' SVER , , Scal @ Scan 3 TUBE F12 Tmpr < User phAR phCH phDY big phQL c phTR phTR