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!!!!""""####$$$%%%%&&&&'''(((()))****++++,,,,---....////000001111222233333444445555566666777778888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 11c7b1db07d546e9ae93f57960802742Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-25_13-59_00923130Seq1279612796_Steier_K1320_psbAjmadera96-Well K1320_E4_psbAKB_3730_POP7_BDTv3.mobSeqPop7z\[\^ckvyvvx|}vokkou{~ysnkijlpw~ *9GRXYVQLHD>4&5XEJS#Umd;K3Ctkm{a%j~( K  1 7  k:{ 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(,(  %$ $%             !      #&#  $++%     $#    !                       !%%"    ):CB7& (+'                 "(&  %#  &(#    %./)         @4A&A=~AKB.bcpKB_3730_POP7_BDTv3.mobtermAGTTCTTTCTTTTGTGAGCAGTAGCATTTTTTATTTTTTTTTCTCTCACATCCATTTTTCTTTTTTTTTTTTTTTTTATTACAATTTGTTTCATCTTGTTCACCCCTCTTTTTCTTTTTTTTTAATATCAGTTCTTTCTTTTGTGAGCAGTAGCATTTTTTATTTTTTTTTCTCTCACATCCATTTTTCTTTTTTTTTTTTTTTTTATTACAATTTGTTTCATCTTGTTCACCCCTCTTTTTCTTTTTTTTTAATATC*=NYl{ %3;IR\fq$6COVahry0>GV^lw +6@LXdm|!.=LWfq}+2=HS^jw*=NYl{ %3;IR\fq$6COVahry0>GV^lw +6@LXdm|!.=LWfq}+2=HS^jw                                ^P+,WQ*Z ,a;%)7:CaF&AI0"6^JJ2%A\=,0B>K<]aK. ]0ZZ6)BJ0<L3L?F_)54SO9*+L=_T\`@3-M+! 9+9EXSA+C^B5"4 F}K:SjY  r UgL0 `   y=P=> ( h ^1}zav$ALb' +-Z&0/.SrR;f/!v l e  fy|mD g;481c*K+@46>a`c%9vTvj];e~zS&HXc`scc&\J-?B`g-SG:%9 ~In"ps4,p'BdMp rEU>?T|Uppd8%IF~CEX(JI<D }'#'1F'-$d"q5f^b@Voq 5 i >vUA&e>skPN*'Z,% TD.,a@LH|S{ W!>PCCCCTCCCTCCCGTGTGCACCCTTTTCCCCCCCCGCCCCCCCTCTCTCACCAACAGGGCACCCCCCCCCCCCCCCACCCCCTTTAAGCCCCATATACTCCTCGATTCTCCCCCTCCCCCCAAAGGGCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-25 15:44:24 -04:00AEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS"BufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA #GSDATA #G31DATA #G{DATA #GDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FWO_GATCGTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOIS P1AMP1RLP1WDP2AMP2BAP2RLPBAS PBAS PCONPCON PDMF3PDMF PLOC PLOC PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N% SCAN3SMED SMLtSMPL%SPACMSPAC SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCH phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS"BufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA #GSDATA #G31DATA #G{DATA #GDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FTab$|FVoc$kFWO_GATCFeat..$=GTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOIS P1AMP1RLP1WDP2AMP2BAP2RLPBAS PBAS PCONPCON PDMF3PDMF PLOC PLOC PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N% SCAN3SMED SMLtSMPL%SPACMSPAC SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCH phDYbigphQLcphTRphTRP'd}|(Hfz@jXa*`#|nh-u?[-0#Osp_0_9v8ZIYW<|eN$&i[D:0M,m(o~%Md02R P,hzp!tY`-'Icf]h_m7^d`7Y{v]qoUYoOyl}$r&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hDM=VrTH&zqru+!hs|O1i~?z;{W8<)mbo)6s6IxO\JpSbwEiv5&o58J.:)'C#x5ots/H1C:2#%=c/a}ayHbc\S.Tqt6IEUYC~n{[._LbR/"E.Om,NeLVWY+a:WkeC.%oh^5Q1V?|&@8je]i^mc~=TgWW' *8/%zoqfTv4*un,T(k"T'ogLc{Xm|3HF)SE8n\qo(k&Q6yk%&`$j *MOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkh<;!d:B.7 TSe'8um(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT3 #pJEa#;{U,Z4Ei5TT3T&#ONx8DTR"S"E<\AEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtAUDTjj2B1PtB1PtBCTS"BufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA #GSDATA #G31DATA #G{DATA #GDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FTab$|FVoc$kFWO_GATCFeat..$=GTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOIS P1AMP1RLP1WDP2AMP2BAP2RLPBAS PBAS PCONPCON PDMF3PDMF PLOC PLOC PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N% SCAN3SMED SMLtSMPL%SPACMSPAC SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCH phDYbigphQLcphTRphTR