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S Z L X Y : Y _ 7 H C < , X M U : T ; A L 8 < + F F W A G E 5 % N C S > : * 0 ? 2 ? ; @ = 2 3 = B X 6 : = Z > \ F I < F I L C @ = 6 N C P J A , 1 F S / 1 U R P 8 : Y M W e 5 @ E Z a . ; _ \ U T T B O P E ? @ N E @ U H I E X G M J M L J Q ; B S W G O E 4 ; Y N [ Z J ; K Z T R L T c V [ N Q < T R _ > E 8 J N [ @ Q 1 P D N > R E I 7 ) H = * D @ _ Z I J 8 H E G E K S R R 8 ) L B < A Z T A @ A : W O B R d < G D W ? ; D < ? 9 E P 7 / 6 / = C > Z D H L E 4 0 / . 0 B O N M S : * H D B 8 @ 3 ; 5 A 1 7 1 8 8 K \ B 3 = $ D O V O G k ; F 7 > ; D 7 7 & ; , H J ( 4 i @ F ^ O = @ 4 6 , G > ] > < 5 4 9 : : 3 H % 8 Z A I ; @ J t 6 @ = > @ L 8 6 F M > L E X M < 8 < 1 @ B $ 6 7 . E 0 - = @ G 9 S = G N M H W 0 ( & : H A M K 8 : O J K J @ I _ B E J H B B A R 6 2 = 5 A G 8 ? 9 < 3 S H 7 A @ - 8 J ; ? 3 * A S L ; I K E 4 - 3 E ? I I C 6 H 7 O < > ? 7 < T > ? 7 5 = C ' Q 3 - ) 4 < J P = 5 F 5 = Z ? 7 A 7 J L R < 1 M H ? F B > 0 E > A P B A O D 5 M 7 C W 3 - F 7 5 $ O M 5 + & 0 9 K F ' ; 7 @ 0 L C 5 ) 3 F I > J ( 1 K : H 0 C E B / a C E 8 / B K R S C W Z \ J < 7 I G @ 3 5 6 . E ' 3 R $ E F @ # 4 2 ( 1 / : 3 9 F , ) & ( 1 3 & I > ? 4 % ? E @ D 4 F @ F 0 7 I 7 R < , 8 B @ ! 5 > ) ' R C 8 ! O I = J E 5 3 ; F F % 7 5 3 9 J K : 1 ; 8 F H @ I > 9 D 8 L 2 O B = B < - 2 Q ^ / > 4 F 5 B 1 D ? R @ / E ; 8 . ) > @ . 3 3 & L c P > < A < Q * Q ? 7 K 9 + H H D D . E G < 6 > 9 T : < P G ; B F ? D C ! 1 2 S C < / 4 ? K 2 A B K ? L D ^ 8 ; % 9 K > 0 7 3 6 . M D - M S Y P F , 1 3 9 F A L 5 N O L V J M 1 B ? = C 5 D K 8 M @ . 5 1 5 0 @ 0 U [ L O d M 8 E G O I Y A 0 V B @ 7 L J S W I Q P T h \ Q R D H _ T h _ S Z 4 D F ^ b [ [ @ Z T V V a E T s \ _ X V K Y w Z ` W [ g W m h _ L U A [ O P _ f I Q N V X 3 [ L b 4 L C J A h ? U A e J H ? I V ^ W L o N A ] A 4 B V G G U ? , E G H Z I _ 2 D ; N V J 4 [ < R ^ 8 0 4 ; 8 J W T > O ? ? > G = N , - J @ D I F I M - > P 2 ? 2 Q D 3 K V 6 ; > F : G M H W 1 - 9 ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fca7c15511524723a35a2b6cd6850233Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
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AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-25_13-59_0092 3130Seq 12796 12796_Steier_K1320_psbA jmadera 96-Well
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l y@AAAA jKB.bcpKB_3730_POP7_BDTv3.mobtermATTACGCTGCTTTGCAGCCATTCCTCCCTCCCCCCCTTTTCCCCCCCTTGGGGGGTCTTTTTCCTTTTTTGGTCTTTTTGTGGGTGGTTTGGGGGGGGGGTCCTTCTTTTTGCCTTCTTGGGGGTTATTACGCTGCTTTGCAGCCATTCCTCCCTCCCCCCCTTTTCCCCCCCTTGGGGGGTCTTTTTCCTTTTTTGGTCTTTTTGTGGGTGGTTTGGGGGGGGGGTCCTTCTTTTTGCCTTCTTGGGGGTT & 8 G Y e r &3>EP[ku"5FRar *8DQ\kv$3=JQany %1@Q\q|"/<HQ\jw & 8 G Y e r &3>EP[ku"5FRar *8DQ\kv$3=JQany %1@Q\q|"/<HQ\jw
J )
D . $ $ D S 7 ) , 6 E
5 9 a / H G 2 " + , H ! ` C ? T ? * E + T
X d K 7 @ ! E H F = 4 4 K P % D 4 a % ? c N , 0 8 & " $ K V ) 3 # I L S V b : $ D A Z c @ ' 4 $ ic(CizS%n$\2.zm7\9G[ pAL 'iL]` $dDLk
C}tq
\ kd:0v= }# @*;]Zcf8 Wbe v @1;=
`ELT!u Q>H #(<U - e T 6! vh l5p 8 Xo &|jp T5: d[w7=jB d,8 d* RT
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m a:|p 8 ' / Dg7
_ Y8 y+ w - 6 . p"WM F IZI tY BcYX R Wi k !! {({'!FtCGGTGCGGCGGGGCGGAGGGGGGGGTTTCTTTTTTTGGGCTTTTTTTCGATTTATGTCCCCGTTCCGCGCTTGTCCCCCTGTTCGCCCCCCTTCCCTCCTCTTCCTCGGCCCTTCCTCGTATACGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-25 15:44:40 -04:00AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA # G S DATA
# G 3/ DATA # G { DATA # G DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FWO_ GATC GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ X LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS
P1AM ~ P1RL ~ P1WD ~ P2AM ~ P2BA ~ ~ P2RL ~ PBAS ~ ~ | PBAS ~ ~
PCON ~ ~ p PCON ~ ~
PDMF 3 PDMF
PLOC ~ PLOC ~ PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N%
SCAN 3 SMED
SMLt SMPL % SPAC M SPAC
SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH
phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN J APXV 2 APrN Ƿ APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT CTID [ CTNM a CTOw y CTTL DŽ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA DATA DATA 2 DATA # G S DATA
# G 3/ DATA # G { DATA # G DCHT DSam DySN e Dye# DyeN p DyeN u DyeN z DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT % FTab $; FVoc $* FWO_ GATC Feat . . # GTyp ! ! D HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ X LIMS ! ! Ǎ LNTD 2 LsrP a MCHN MODF Ǯ MODL 3730 NAVG NLNE ` NOIS
P1AM ~ P1RL ~ P1WD ~ P2AM ~ P2BA ~ ~ P2RL ~ PBAS ~ ~ | PBAS ~ ~
PCON ~ ~ p PCON ~ ~
PDMF 3 PDMF
PLOC ~ PLOC ~ PSZE ` PTYP PXLB RGNm S RMXV 1 RMdN = RMdV 1 RMdX F RPrN ! RPrV 1 RUND
RUND
RUND
RUND
RUNT
; RUNT +9 RUNT #9 RUNT *$ Rate 8 RunN " " 1 S/N%
SCAN 3 SMED
SMLt SMPL % SPAC M SPAC
SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH
phDY big phQL c phTR phTR eZ>\&bD8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|$q_7U4~bUjZ8~Uz`_^3sVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*t; L#1i`;i'SKmvUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!#%`8pSsma7yMR9MbELIV(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+Vp5OnNw\O_LB"DHK#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL