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!!!!""""####$$$%%%%&&&&'''(((()))****++++,,,,---....////000001111222233333444445555566666777778888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7e6c85054b154b4c9e9cde388e8e7479Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-25_13-59_00923130Seq1279612796_Steier_K1320_psbAjmadera96-Well K1320_C2_psbAKB_3730_POP7_BDTv3.mobSeqPop7z,,--./1110.,*)()*+-//0/.,*))())))('&%$$###$%%%%$$$$%'())(&$"""$&)**)'&%%&''(((((('&%#"!  !#%'())*,/4:BKUao $<RfwoV;q`SIA;630.,++,,./012455665557:=@CDDDFKVey(Q{iAnYJ?:89;=====@GRbwcu> Di8iWMGDBCEKTaqL#mm(9lN>9:<>?@ADGKPXdy Z3eu`)q8nK5*&'*/4:CQf20xt)x!_:!        #%%$""#&,3<FNRRJ>34R!6U _sim"#Z]$5#e0  6d=X r;  'S~`.0{yX"mRuq'    '2<>7% GJSM      .n+\P  )bo^Q  %%Nd%t3 ) !$$ =qFrd1\!  #(& W=P(c%ouF@  @W}W    !$$!    (5@A35i; |      1yHr U! &01) E 9@;9&!S)UM  #0=D@-Bqy-#'#TA~;   $'+055, eXn8=M&     )032,%    &19>=93-' M7c"        I7<X%      D*.O  >P&m0      A>K0A,lGI2 "   #$# &]=fg<6>a/ 18:jQobWM-/J|7K5P]F_3KaJJY2H><Qn'   )wh+< *Q 3eU(     G+.R"  J$ D:yw& %k^we/O       "" #$ 3Xcp.4`q$   *VBO9w;!&"[- NdR/&W.   TNRq~*  *i!v1t0      !"!%,4::3$5~\, Gs0   '++(!6_&*lA**>^s8NsiIY=""!#)1894*&7JY`^R?+      A|8 /]*4:8-b1{;L >M\;{qg^WQMIFC@=;:98752/-++,.1356789<@FLRX]bhq}"GpBa|pU<&:b!Lpb1x7}D*c'kHcldIObiO=0($"!!!#&)-034555431.+)'''())(&%#! !!!      %+157767:EYzF#)-Co{]*{{N0 !#%&''()*,-//-*% #1Hji?adGf zI(  %+/342/,/?aZL-&-@S0 %*,+'#$/L<3%(I fP. "(,--+)'%$#%(-3:@FIJIGD@;5.'&0O{&&}Q1y*W#$Y#/lWp!LqRJKKC4&)OSDrN]wl@  &2<DGGC=5.("      831\C+o`N %08:94.&!##! #&(% @|sI$  %_Gt <J"(/8ALUYS@$ CM   &-11+!    "'+-,)%$%+6BKLB1 @/ xkZqQ.?>T &/48<@GMRRL@.4nF.hd#M*1s zll&  $+2;CHE6z&  { *>Q_geYD. (S"8-I!pPZ_}C-0;@7!]^sUUw{m? '5AGD7! D;lm7&"4l!   #'&"      &&M;<ue44j00?HG9}<Bgl2B"2DT`iouwr`@F& n ';LVVM@1$"&('$$),,)#"(-.& TDNU`m  !      !)) v\6:  &-34,Gv<Xelf !&+3<BC:& :g= A*$P-Ayq7#WeXg*&w&7)^N F ""   #*) !| m- 850x7G7W !03*Rc lu$_~jg  !$"        F }WT]T B   #,0/*   " n"x&   $),-,'    !" C( XI}~,  7|.vXR):?6" '{|K\'5P     'dYzIV    %+,' L6a "^frR(pn+1I   \9,S      '+* #(ReM4!   "Y;a]1Q ;Ysy`9; }4c"   ! ${K^2 !#$!'j+7u;,Ex\7   /Zm@  $*/5<DMTUL7%~ `;;     -w7? UQA}7?     ""  2m' K!*3:=90$!9jMr4f]`it~~vpnr{xrykYc3-W% {wW ;P\_\WQI?4& *AYksoaK2        $(-28?HR]iv ;Ws)Pt4I[hquvrhXB&rK#}W40Oo%29:5)wst{}V-sXB1%  !""$&*.28>FQ_r3j 6UiphT2T]A, "&,5@Qh+w#y1F@ B,qL2! '-39CQf\Y UNjVQ\vap%0#&J\V3:erB" #&(+/9Lnar ,P'.S{m/*l4 #(+-.//.,)&(3O7>7:=,r@$#5YSJ .!l6Avlq r8 8u;!G]"   JiX}U!  (1894' *rGVH3y F)<E5eT WW   Q=x:7o5Q <.2BW~B:d)N ! W>WaJ^  5#>?^A3u}Ls>0|ns#*8 "*,%]^5fKXzWi$ 1)&|XBpCfX   D0V).    "()%- PBG      (./&]`/x0}\@)K;gv%14+ ID0vR>H).\:(-( ({?"Oxp!Rx2Do-xc#<q% VZu[1>      1Pc@T5k  `YGpRqq"!4E7\C1fcL#`C   "-0) K[RJ !+23*C\[\ k[T d1i0#\I   %G)X#B0<?Y%* '~es75J      *.)70j}' %T6x\z-h U'=oxi   crtof[   ! vQII3Q|uI1J?U'E~r3*%W$f(H]AYBrF >Nb }$ *-' fI,6\  :4.`Dg1Wr;(Rsoic 8v Jg!  ! ; -#Q<d6am  7VV}1   *~=>+   USe|}Ah%  (qNPO7H>a:Ra 2mAc  T:b_3M   #[@hh?]& ZsTB      8Bv_"   3x/xRP      ;zLeV#H  (aIqoA@AbCC J 7?%c-     1s"iJP   8[{|\:    !!"""####$$$%%&%%$$$$$%%%$#!  GPa}$4=GUcouvsj\J3}\:t_M?3+'',5CTjJ~$]<m d2G\>(/D\wlCe@{wutssssrqrsw} 1_ ODrh8G{<wh]UPMJIJKLNOPQPPQRTY_gs3n.dn:q.xcWQNORW]dlwH^9 5'EZzlQB:889:<=@BCDCBA@??><94.)$! "#$$$$$%&()))(&$###$$$$$$$$$#"  """!!#'*-./.-,+,-/13579=CNa9e 8IB.=ga  :v]QNORTVWURMIJVv A4iO, "$%&(*+,,+*(&$#"""""!)MGhT(w?      '.351'C,lT =  !!  &**#@+AB2G    "(.37;=??@@ABEJQYbkrtn`J4+<wx%IdZ|*    *4:9. HF`#=     &./( M`-MzfB2|-   $+27<><2 00B<=   !    ",47/ Kq1Oj(4C'<F?)MX[1 $%! #&&$         "%% GItyWV,8:3# o<{`'&M$5                  ## NIT):       &.0( GKx}WT   %(% !q|lj>$% '/573( KNkQ0C    $'% jhPuSt{.  %,022-#.BK"I            FTpl4            "WBu^a$:k7v6   #Nn; 0b [-     %++ i\SU($~; 6JQK;+"$4Ot#AOI-cQ]R{l8uJ1&#$#!#(.47850*$$+153,  /bCvr=e7 $3BLOI=0)4V;[rE.),141+$%.7=?;3(  5 B@DA OA7AAKB.bcpKB_3730_POP7_BDTv3.mobtermCAGTCTCTGACTAGCTGCTGTTGAGTTCCATCTATCAATGGATAAGATTTCGGTCTTAATGTATATGAGTTCTTGAAAGTAAAGGAGCAATAACCAATTTCTTGTTTTCTCAAGAGGATTGGTATTGCTCCTTTATTATGAAAAAATTTTACATAAGTTTTTTTTCTAATTTCTACTTCTACATAGTTAGTTATAAGGTAGGGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGGAAAGCAGTCTCTGACTAGCTGCTGTTGAGTTCCATCTATCAATGGATAAGATTTCGGTCTTAATGTATATGAGTTCTTGAAAGTAAAGGAGCAATAACCAATTTCTTGTTTTCTCAAGAGGATTGGTATTGCTCCTTTATTATGAAAAAATTTTACATAAGTTTTTTTTCTAATTTCTACTTCTACATAGTTAGTTATAAGGTAGGGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGGAAAG3D^ev $0?N_hs $,8CQ]dq} ".8HTalx'2=HR]it&2=IR]iu'3?LXcnz&1=HTanz !-8CP]iu    & 2 @ M X d p }    ( 5 @ L X e q ~   ' 1 > J V b n z  " . 43D^ev $0?N_hs $,8CQ]dq} ".8HTalx'2=HR]it&2=IR]iu'3?LXcnz&1=HTanz !-8CP]iu    & 2 @ M X d p }    ( 5 @ L X e q ~   ' 1 > J V b n z  " . 4  ! ! .*6#& !>/ />38,%8;>;+ 3;1;>>;;,>>>>>;6.;;;.>';;;3>;>>>1;>>;>6>3>;611>>>>>>>>>>>6>>>>>86;;.>3;6066>>;>;6>>6>>>>>>>68>>>>>>>>>>>>>>>>+16666>>>868;>>>>>>>6>>>>>;>>86>>>>>>>>>16>>>>>>>>>>>6;>>>>>>2>>>6>;;86>>>>;>+2"*2>;>>>3;6+-".    ! ! .*6#& !>/ />38,%8;>;+ 3;1;>>;;,>>>>>;6.;;;.>';;;3>;>>>1;>>;>6>3>;611>>>>>>>>>>>6>>>>>86;;.>3;6066>>;>;6>>6>>>>>>>68>>>>>>>>>>>>>>>>+16666>>>868;>>>>>>>6>>>>>;>>86>>>>>>>>>16>>>>>>>>>>>6;>>>>>>2>>>6>;;86>>>>;>+2"*2>;>>>3;6+-".  L\7N.aXV!BI&6-(E/FRb?RF4>;?  AV-=. I#F *GA"F !TZ6O0@--K1 3 @@JJ`K-IbT_ER5R<33RA7`^T!% /!P7\KG2H<*X]%4`5OR](6=]W$0@WL#;D:>; M7W4KdS/;5L'9VVBH>. <,'!%I Cd2%0S:9%!/6XQ]*-1-C0SX4:b <-S/++,/" c]qydEoPS~~|uj  W<)MK -(xFJpS\,+F&z;n5R[};R4FWU=T+|),jZ%g*J4L01016?t3dz^2i9dPru +mb<  KWL/F %cBL}mr\ @zkYdmS>uxA\E@Fx++##       xxxx^ 4:/ppa8H,Wad}'`~$G]d.^JJ4%y E>=xAKb8+}{dUCUQ@MsFQWQKn+F\MRnd\Edss :a4 ,5q:dndE9:M6!KK Hi)M dj)LoU{kCX?LNP!0k"./ON@A3,C4(]&?I&IB\-:4 91LAF8 B,m=-!Z662eL&)33"C+=O'B#'53??J ;- ((+?L <!I3^.LO23W)G@W, '%:-  D;. '-A!T @.,&. "4G?34I<F9!3A.R98}8=7{GjFlLnL cACACTCTAACAATATGCGGTGGAGTGCTACATATAACTGAATATGATACCGCTGTCATTATGTATAAGCCCTCAAGGGAATTGAAGCACTATCAACTACCTCGTGCCCTCACCAGAATAGAAATAGCTCTTCCATAATGACCCGTTACAACATAGGTACCAAACCTATTAACTCCTACTACATAGTCAGTGATAGGAAGGAATCCCGCATATCCCGTCTTGTAGAAGGATGGTGAATAGTGATTCTACAACAAAAAGGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-25 15:44:14 -04:00AEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS'BufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA $ISDATA $I57DATA $IDATA $IDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FWO_GATCGTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~&jLIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOISP1AMFP1RL>P1WDNP2AM#^P2BA%fP2RL!VPBAS"PBASPCON:PCON6PDMF3PDMFPLOC.PLOC&PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N%SCAN3SMED SMLtSMPL%SPACA|fSPACSPACA|fSVER4.0SVER 'SVER,,Scal@Scan3TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS'BufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA $ISDATA $I57DATA $IDATA $IDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FTab6MFVoc6<FWO_GATCFeat..6GTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~&jLIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOISP1AMFP1RL>P1WDNP2AM#^P2BA%fP2RL!VPBAS"PBASPCON:PCON6PDMF3PDMFPLOC.PLOC&PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N%SCAN3SMED SMLtSMPL%SPACA|fSPACSPACA|fSVER4.0SVER 'SVER,,Scal@Scan3TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTRa,pEnO"#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? VCCHY<6(mu y-WLa)&:\.~x|MI9?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3WAd@EaZL)E^rkrOW9;(dC18{ZWFrbT;fQGKekx&#v3"qo,D`&Tj1t9z"DEoh90S*o_cM&+#u?GG5)ljD_P?Yf]4'Uw\Pcc{\A'ZvXTP>I_n@(Zf.kRr+4#3y4E^h9r4uoak"T'o7LA;mq;;9VgO(_y"`k:!.tkSY4E2 (#>+6hj"bpTJyV'GGV_6.2Kr)YW [xinJil]ZCd]'DZNu@v0kc "V&S$~=}F)h>;-T)RJTZmU)P? sIe]UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4r;yr.=:!)Y,\wbrmzp0q_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|Gl29E:#t;MjlV:v4xe:^*!t p7_lnqR9JK[Ylid?O>JWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0x9zG@}d>4"AEPt3AEPt3APFN JAPXV2APrN ǷAPrV1APrX}}ARTNASPFASPtASPtAUDTjjD`B1PtB1PtBCTS'BufT CTID[CTNMaCTOwyCTTL DŽCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA DATA DATA 2DATA $ISDATA $I57DATA $IDATA $IDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FTab6MFVoc6<FWO_GATCFeat..6GTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~&jLIMS!!ǍLNTD2LsrPaMCHNMODF ǮMODL3730NAVGNLNE`NOISP1AMFP1RL>P1WDNP2AM#^P2BA%fP2RL!VPBAS"PBASPCON:PCON6PDMF3PDMFPLOC.PLOC&PSZE`PTYPPXLBRGNmSRMXV1RMdN =RMdV1RMdXFRPrN!RPrV1RUND RUND RUND RUND RUNT  ;RUNT +9RUNT #9RUNT *$Rate 8RunN""1S/N%SCAN3SMED SMLtSMPL%SPACA|fSPACSPACA|fSVER4.0SVER 'SVER,,Scal@Scan3TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR