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###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b9ac64d133c141559f1bab361cdf87e0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_H2_Hydrocotyle_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zX\dr)7Ja}0@FB8-$"%-5;<8/$ zmbZVUX]elolcTB1" $GqQ?h "g)F_8{}j[SRV_l~#k|0up1;wL%~zwv{|}~|uiZJ=40//000138@JU`gllic]WOG<.      &9Pn?}~O_B,'5EUfvMF1!N7j>    $'+-0121101257751+%   %1:ADED@;4-(&)/7>A?6*    +O%uE.|nmTVL/IMXe!    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(P0MO6}y6Y`Hg>%$*/221/,($-Jr uS9' &Bj ,!rN6'   +RvL. "''"     #NDa`Epb[UM@/0X ;Y]I"kQA7325:@GLONIA6+""*18;:5-"     '.33/'  #EuCPF&a@*  '2>IS[`a_XNC7.'#"$'*,--,*)'&$!!$&%"   0Hf}Z=)!#!                                                                                                      A"@A ~A:CA~KB.bcpKB_3730_POP7_BDTv3.mobtermCGGGGTCCAAACCGCTCTACGATATTTTTAGCCGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTTTCAACTTGGATGCCATGAGGCGGGCCTTGGAAAGTTTTAACATAAGCAACAGGGATTCGCAGATCTTCCAGACGTAGAGCACGCAAGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAATGGATAAGCTACATAAGCAATAAATTGAGTTTCTTCTCCAGGAACGGGCTCGATTCCGTAGCATCGCCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCGGCCCCTGCTTCTTCGGGCGGAACTCCAGGCTGAGGAGTTACTCGGAATGCTGCCAAGATATCAGTATCTTTGGTTTCATATTCAGGAGTATAATAAGTCAATCTGTAATCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACATTATAAACACGGGGTCCAAACCGCTCTACGATATTTTTAGCCGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTTTCAACTTGGATGCCATGAGGCGGGCCTTGGAAAGTTTTAACATAAGCAACAGGGATTCGCAGATCTTCCAGACGTAGAGCACGCAAGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAATGGATAAGCTACATAAGCAATAAATTGAGTTTCTTCTCCAGGAACGGGCTCGATTCCGTAGCATCGCCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCGGCCCCTGCTTCTTCGGGCGGAACTCCAGGCTGAGGAGTTACTCGGAATGCTGCCAAGATATCAGTATCTTTGGTTTCATATTCAGGAGTATAATAAGTCAATCTGTAATCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACATTATAAACA!,9GOZair~(5CN\et#1;JXcp| +8BO\ix"/;CNZgs~ &4AKWbnx&4ALWbnz(5BN]gt(3@LXcq}   % 2 = H T ` k w    ' 4 ? M Z e q }  " - 8 D Q ] i t    % 1 < H S ` m z    * 5 A M X d o |  #/;HT_kw  ,8CO\ht&2?KWcp} %0;GR^jw+6BO[gs} &2?KWcn{ $1<GS_kv+7DP]it&3?KVbo{ ".;HT_jv,6BNZgs(4?KXbnz %0<HT`lx#,7!,9GOZair~(5CN\et#1;JXcp| +8BO\ix"/;CNZgs~ &4AKWbnx&4ALWbnz(5BN]gt(3@LXcq}   % 2 = H T ` k w    ' 4 ? M Z e q }  " - 8 D Q ] i t    % 1 < H S ` m z    * 5 A M X d o |  #/;HT_kw  ,8CO\ht&2?KWcp} %0;GR^jw+6BO[gs} &2?KWcn{ $1<GS_kv+7DP]it&3?KVbo{ ".;HT_jv,6BNZgs(4?KXbnz %0<HT`lx#,7    #' %2 $ 66#$4/"$4&*>>//8>86>>>//>6**8,8;,>;,,+2-3;>>;;;.6>>>>>>>>>>;;31;8>68;;>;;.8;;.>8;;8+8>>68;>8>;66;>>>;>>>>3+3..1>66;>>8;>3;>>>>>>;3;;8;;;>>>>;>>;;>>;>>>;;;;;;2;;>>>>;;;;;8+82>>>;>;;;>>>>>>>331&0;>>>8>>>>>;6+8;3;>>>>>;>;;;;>8;;>;2>>>;;>>>86;;;>;;88>;3;;;;;>>;;81+668;>>>>>>;333328;.8;;>20;8686>;;;>;>;;>33>-;>>3;88;>>>>>;;8>>8>;>;;;>>>>>>>>>;>>;-;;->>2#;;;,+8;3>>>>>>>>;;>>>>>>>>>>>>>>>;8-8 3*8&>8>>>3>68>>>88838>>3>8>>8388/& &*>&3888888'%"88888.*>>88>8>>>88&.2.24444+..+44.(443#'##+4,04)'*%//24/4 .*  &  )4!    #' %2 $ 66#$4/"$4&*>>//8>86>>>//>6**8,8;,>;,,+2-3;>>;;;.6>>>>>>>>>>;;31;8>68;;>;;.8;;.>8;;8+8>>68;>8>;66;>>>;>>>>3+3..1>66;>>8;>3;>>>>>>;3;;8;;;>>>>;>>;;>>;>>>;;;;;;2;;>>>>;;;;;8+82>>>;>;;;>>>>>>>331&0;>>>8>>>>>;6+8;3;>>>>>;>;;;;>8;;>;2>>>;;>>>86;;;>;;88>;3;;;;;>>;;81+668;>>>>>>;333328;.8;;>20;8686>;;;>;>;;>33>-;>>3;88;>>>>>;;8>>8>;>;;;>>>>>>>>>;>>;-;;->>2#;;;,+8;3>>>>>>>>;;>>>>>>>>>>>>>>>;8-8 3*8&>8>>>3>68>>>88838>>3>8>>8388/& &*>&3888888'%"88888.*>>88>8>>>88&.2.24444+..+44.(443#'##+4,04)'*%//24/4 .*  &  )4!'C_6YN@5+:!Y^< U3R_0,98,TC#Q0T)K 5QK=15)$Z(*OKGW_* /T)QN#ANP ZR T/Z,:$YBV6 >S+d2X  HJDGX$RR;D2@' \*!8a*;4*E:?HZ53F0 AB#cV  8"/KGdH!2ZO?)@F\8@;-c.!*.QP\&]Z3X-X"-3 0GdDX8&E(`KV[A$,[a@ Y$,42VX6=TZIQ;2\F X+W9aE46<4.5 3 Y:M`#>X9FUG\aD/-L=WN?%/7!F^C3OY0Z<^6`6S\WP0)</N #WL>42E73` ]ZN,2'@<dN$DOZ T ::9cK  cBSB)Y:4` B;:$,7@0D_b2>(//?#3"V[E,L(@Y(R1`[?_XA*0I]9Y' B_FV3* E QH*,5[V4cY> @GI3E! :&2Y@ K[$L"a!' 3=P2Oa\^\D4dKJ1%<LcR2{d8?YD. ,U7/ 5l L!3kRB, 7]d)b!3bxv)y?;f|\nh|-9xA|[+j?iYzu`vK?n*ypi>I?-/C C]q^+y A \j@63 1ZG~Y<GX( ~k/vSX% g[4|(*XHZ  R'2QAubUB)Z& uX|!a/PgA|X lL DeMwxb!Ip<|x;Q roh2\(1`u9{ C:^N e| .tMVq9A9Fy7,Ce@oyy_M54)r]>3Gq#55b2?,(F,H8]ICWGp[4N3=TMui@3GH@?ydb@Np YY {*i `J5a3aP" bozJerA=[=BJE`. C*>#%-)&  L%#!        $$    #+(9?" '-   /)'&!-8'&g-?" +=, *1%;95' (?/LA3?H-==?+&70`=;A4(1@ /5?4!5@  p ( H(((( (j,yj8T yXp|p@mpUudV_+pd@2p!g+gMS 3p)yhp-Zj Lq@WpjR+a/%e?^U, rJ8b#5\JU'9p%WvS<PYD#k|qN>^t9$J-k!$r 8dHm%VA/kX{dM:Ps`npwO-j8^ WAz2Ji RZ%BCU;pj.D ,S~{AJ8\O%1py!c@^D|V kV Q)$_ij6c6e d.o'P( G(S*-.V8?>E/Z8KC[Jvpj;?3~0/V" 4+4<5c5M(1$+=nRI4&V,>:;IH)WK1"zJ*.?=O/=18$,^8 6#?1hEM3(h\$"$9F[`1#62Q=![@.F-H"*++(P!f<"-&79<68/(9 `o!-.GQ)41\51;#(.E1CZ#_^@'"1Y\YA/1xOl (7wX36'@)RO.!*w*Q'_:Z8^Lj3R8)>I3"7%0M.?atN#6m9|8F_R%l.g8J e:%' >$W@zW%;"`-II0x0@%?k` ;$6"'H4%$iKJ<_}2Y/7.7x-+DPd/RW%RPH7+@p-/:9c )',- *)4Q~` [5*1lbAJ;40QU4-)"oU3'>5:]C->=X)F]=bnlG@Q1Q>v\??U(+S&!{jqZCLC@dzSQ6VeD}<ev6k}^I?rh.5p Cg=??Fa/zGAAAAAAATTCAGCGCTATAAGAGAGAAATCGGATATCAATACGAAATTAGAATCCCTGCACAGGACGACAAAACACGGACCTTAAAGGACGCAACCGAGGCAACCTCCAACCCGGGAATCCGCCAGAAAAAGCCAGGGCCGTGGGTAGCGGCCAATCCTCATATGGGATATAGAAAGATAGGGTGGGCAGAGGTCTTTACCGGCTTGGTTTCTTCCAAAGGAGGGTGCCACAGAATCGCACCTGCCTCCGGGGGGAAAACTCAAAGGTTTGCTTTAGGCAGTATTCCCTTTCAGTCCTGTGGAAGGGCTTGCGTTAGTTACCAGGATAGTTCCATGGGAAGGTCTAAGGTTGGGAGTTCAACCCTTCGTGGCCCTATTCCTGGAAGAAGTGCAGAAACCTGTTGTTTATATTTAACTTGGTCAGAAAGTTTTTCTTGAAGCCGAAGAGGGAAAAAGTAAACTGACCAGGGATGCGGGTCCCTTGGGTTCCAGTTCCGAGGGTCCCTAAGTGGGGCGGGAAGCCTTACAACCAGGCTGGAAACCGGAACAAGGATTGTCTCCGGGTGTTACGTGAATATTCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:23 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtyASPtyB1PtxB1PtxBCTS3cBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 63lfTDATA 63lf(DATA 63lf-DATA 63lfDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~1LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISbP1AMG[P1RLGP1WDG!P2AMG+P2BAGG/P2RLG&wPBASGGPBASGGPCONGGPCONGG?PDMFTPDMFyPLOCGPLOCG #PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%ZSCAN@SMED SMLtSMPLTSPACArSPACrSPACArSVER4.0SVER 3XSVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCHphDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtyASPtyB1PtxB1PtxBCTS3cBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 63lfTDATA 63lf(DATA 63lf-DATA 63lfDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabAFVocAFWO_GATCFeat//A~GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~1LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISbP1AMG[P1RLGP1WDG!P2AMG+P2BAGG/P2RLG&wPBASGGPBASGGPCONGGPCONGG?PDMFTPDMFyPLOCGPLOCG #PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%ZSCAN@SMED SMLtSMPLTSPACArSPACrSPACArSVER4.0SVER 3XSVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCHphDYbigphQLcphTRphTRY#rNT/$OY ~?p96UI//`JiOzJ} {&kdA[L25nWsyZKh~8JePS"Fp?(J/Hcz,(,y]18`F7IOo_3@/$9E^E:D+UZ~en?R/FIOb]rDKRm*u!?Xa./l3672_4&blIk(!Y`%]RI1]f\+!@rIwYWgUTHG&/Bc1h67A"yV+L"o&Hmd1I*w&OLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`_dEKW\lWQ7M31pfQ>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W*c3oAa!jv3}H1,Cz#Z|#e1a_WTDr]?'Hrz9Drv4~K3qoI>*?ag5d7m``k:!.tk}Y=52r =&e=.7I 9'kT'_C:j|(5,grOT1 x>eId|NHp&k4NK;25L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@Cz6AqdIqN6`aD/S)&!x_M,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"+%3N+WB9anJWq YI*Yf64?C-~O2[4mg$!ehD5u/03BSmv^LoT.iP""#]eYWjoW`$~~hY8|kN_uw >xP!t p7qf j O5TwARM