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###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 14874d1a73e34568ba81721cb21688b9Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_G7_Vinca_major_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z_`djs;] ?g))" 4T|y\<n^RF:+~qcUH<2+&$%(,28>ENWcoz 7LZcjt 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"'&                                                                                                                                                                '/0)                                   "" @p;AbADFtA [WKB.bcpKB_3730_POP7_BDTv3.mobtermCGGGCTTCACTTGTCCCTACCATAGTTTTTAGCGGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCGTATTTGTTCAATTTATCTCTCTCAACCTGGATGCCATGCGGCGGGCCTTGAAAGGTTTTAATATAAGCCGGAGGGATTCGCAAATCTTCCAGACGTAGAGCGCGTAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCCTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAGCAATAAATTGATCTTCTTCTCCAGGAACGGGCTCGATGTGGTAGCATCGCCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACCGCGGCCCCTGCTTCTTCGGGTGGAACTCCAGGTTGAGGAGTCACTCGGAATGCTGCCAAGATATCAGTATCTTTGGTTTCGTATTCAGGAGTATAATAAGTCAATTTGTACTCTTTAACACCGGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACGATATACACGGGCTTCACTTGTCCCTACCATAGTTTTTAGCGGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCGTATTTGTTCAATTTATCTCTCTCAACCTGGATGCCATGCGGCGGGCCTTGAAAGGTTTTAATATAAGCCGGAGGGATTCGCAAATCTTCCAGACGTAGAGCGCGTAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCCTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAGCAATAAATTGATCTTCTTCTCCAGGAACGGGCTCGATGTGGTAGCATCGCCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACCGCGGCCCCTGCTTCTTCGGGTGGAACTCCAGGTTGAGGAGTCACTCGGAATGCTGCCAAGATATCAGTATCTTTGGTTTCGTATTCAGGAGTATAATAAGTCAATTTGTACTCTTTAACACCGGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACGATATACA !,8CMYfr{,8EP\et+6ER]jv  -7DQ]lz  ,5?KWdoy .7DOZdp|'2>JU`mx*6CMYdq|  -8DQ]iu    ( 4 @ J V b m y    * 7 B N Z e p |    , 8 C O [ h t  ! - 9 E R ] i t   # / ; G S _ l v )4@MYeq} #0<HTamz+7DP\gs%0<IVboz!-8EP[gt*5ANYfr&1>HTanz #/<GS_kx+6CO\hu)5AKWco{ %2?JXbnz $1<IT`ly $1=IU_lz !,8CMYfr{,8EP\et+6ER]jv  -7DQ]lz  ,5?KWdoy .7DOZdp|'2>JU`mx*6CMYdq|  -8DQ]iu    ( 4 @ J V b m y    * 7 B N Z e p |    , 8 C O [ h t  ! - 9 E R ] i t   # / ; G S _ l v )4@MYeq} #0<HTamz+7DP\gs%0<IVboz!-8EP[gt*5ANYfr&1>HTanz #/<GS_kx+6CO\hu)5AKWco{ %2?JXbnz $1<IT`ly $1=IU_lz   :,+1   )) 66)/>>&%8/8>/8>8//8>8,;>>,;8;/;>;>;1;;;81(3>>66>>1'>>>>>;8;;;8>;3;>>>>>;;>;;.+>338.;>>;>;;;;;>;;8;;;>;;>>>>;>>>>;;>311..;;3;;;;>>;;3;;8;;3>>>>;;3;+>>;>>>;;;;;;088;>>>>>>>>>>>;>>>;;;18>>>>;>>;;;3>>>>>8>;>>>>>>>;8;;;;3>2;;++6&.;>>>>>>>;>>>8;;>;>;;;>>;;>>>>;>;;;;..>>>8>>>;>>;;>>>>>;>;>>>>.8;;;8>>;;;;;>;;;>;>;>>>,;;3;>>>;;3.2;>>>;>>;;>;3;;3;>2288;+;;";;+>;;;';388;;;;;83>>>>>>,>8>>>>>>8>8'3&>>>>6/>1388388838>88>88888/8+/88/88+8888888&#4(44*"6>&+>44044+&+444.4.44**'"'24**!  !"2%#"!4!% **       :,+1   )) 66)/>>&%8/8>/8>8//8>8,;>>,;8;/;>;>;1;;;81(3>>66>>1'>>>>>;8;;;8>;3;>>>>>;;>;;.+>338.;>>;>;;;;;>;;8;;;>;;>>>>;>>>>;;>311..;;3;;;;>>;;3;;8;;3>>>>;;3;+>>;>>>;;;;;;088;>>>>>>>>>>>;>>>;;;18>>>>;>>;;;3>>>>>8>;>>>>>>>;8;;;;3>2;;++6&.;>>>>>>>;>>>8;;>;>;;;>>;;>>>>;>;;;;..>>>8>>>;>>;;>>>>>;>;>>>>.8;;;8>>;;;;;>;;;>;>;>>>,;;3;>>>;;3.2;>>>;>>;;>;3;;3;>2288;+;;";;+>;;;';388;;;;;83>>>>>>,>8>>>>>>8>8'3&>>>>6/>1388388838>88>88888/8+/88/88+8888888&#4(44*"6>&+>44044+&+444.4.44**'"'24**!  !"2%#"!4!% **    P0&CS]';\+&3K#D1=@\^_ CA\ dA\RX M cU=II"Ja0Z&*$$6L\Q>IYRVT? >T6M3<(c3I<M6]#`0PNQ^MF8/ H#8>&ca+PVB# 4A8# OR A?&!S@(/"d[aU:T@F;.MO:`=I ;? L_ _$M\^`TYa &V>cD1M^.1,BU5!'Yc:0;4]A8-20'QaL*<K2]V]%PA5I5 .ZW<<S NFPZ^=?_<=<'<;21X9 83"XH$!L8aZ :KM8DbS 3T6S& *c$A N:)( ;&TSB65 0%Z?W. )-aW@O6@;R;:KVW(/d/ZFXND]7(>5ZEVE^VTDbAP Tc@&+ -#"a*";:HTc;Ca!)8\$6X:^U*%;)5O%\Q8.;O<*T? cI ,5UCMKE1 `7P1)MRCN6MM(AS?LJYKY9V)Z Db&X'64K2``I \2VOdP+ m 0 6IXtt;*) 7m ^#& Nut`g~32W5T))nlo5y]QZe  2(6Hy&vo!=?ud~87PTR1 Ads#!5rjVjYgn+ IhoncM"AspG6U>90p(~1+(Mc<QvMF ; ?f~IYzgAEbCTo:oJ g[?iw=#:zQ8xHX Pf<Cqa}Nd 3_iR=k\Rw8j"t-&?QMYKM] &[ K}z|KT`^qA)rw xifqSRBG7<SAlhZATh)vo t+IJl  =bs/ `:CC6<Yjs$gtGaG6?d$B875IE)M-$+*84#.E#       )    R     (  &!  +! ">  ) $'"K,;''$72<9:6::B-F4@;O.  '/"-V87">::4@*@57"?CA)"&'JB@?##""","7W70HJ,",,8,:Z,:VG"7?(xap? ~ bXKx27bG>h}yv^Wq*,]pF* pL8o3pq8dn,VOqj8QKP,,dae #jM 8pdzlNK+Wm<<O&}Rt8ydq{rriRd1d25JN/; vXlsk't$p2~fS#(88a_%B1eSKIJU+!n#<kaM:,pS8<U6  7,pw7!+ Nn dbywpm Ss=~pp*1t _\ Py4@T\m&N\\ORYh[H3d|H54+=\C^:1ETAH2\oXZ|F0\#:UWb/( 7/E36OOPA:TC2F2B3H1C1?/H8>3K@FzG?39}j.Ie?CTMH*&,G%/P4ZM`wj@(6A_'.*;X&pK7-6"7@"1NA/<<@Mn7"3!@;7>*b3/L]DG&ZF'V?<+U_3>#D}%O-+E5$D)[WLhcD/fY{V48#3zU>=;V>'NE>8CTp.05$V'8]&%tOm=gz[MWFmI#)E0Kf7c#*$"YfN*PT@KF,4,K.SOQrg9-jK%LBm_E8I:N^3f3lt- 7DQ;J}?$H#M;QYE(@P/`>D0D9_8l<!4)p.S?@Z@Ln`UXUOLBFT^=$ 9B5N-"@mMN23.B\4@*WJ=[-^U9P&vU$<@DkMy'3}/T;QkNAp1OLRUT0?=wA4fMppI:LzZrs'> |Fhh`<h4B^ rAAAAAAAATAAGTGTTTATAGGATAGGCAATCAAATATCATTACTCGATTTGCATTCTTGCACAGAACTACAAAACACGGACATCGGAGCACTACACAGAGGGAACCATCAATACGAGAATCCACCAGAAAAGGTATGGGCCGCGGTTAGTCCGAAATAATCTCCTGGCATATAGAAACCTAGAGCGGCCCAAGGACTTAACCGCCTTGGTTGCTACCAAAGGCGTTGGCACCCCAACGAGCCCGTTCTCGGGGGCGAAAAATCAATTATTTGCTTTAGTAACTATCCCCTTATCCTCCTCTGCAAGGGATTGGGTTCAAAAATTGTATCTTTCCATGGGACGAATGAAGGTTGGGTGATTATCCCTACGCGCTCAACTTCATGTAAGAATATGAGAATCTGCAGGTTAAAATTATACCTAATCAGAACCTTTTTATTGTACCCTAATTGTTAAAAAGTAATGTTACCAGGGAGGACGGTCCCTTGGGTCCCGGTGCAGAAGACCCCTAAGGGAGGTAGGCACCCTTACAAACGGCGTTGGGACCTGGCATGGGCGTCGGTCCGGGAGAAGAGAATATATACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:40:10 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTSKBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9?r~TDATA 9?r~=DATA 9?r~9DATA 9?r~9DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~J$LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMF5P1RLF1"P1WDF::P2AMFCRP2BAFFGP2RLF>PBASFF!8PBASFFPCONFF.PCONFF,PDMFTPDMFPLOCF( PLOCF#~PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACAoSPACSPACAoSVER4.0SVER KSVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCHphDYbigphQLcphTRphTR"3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtcASPtcB1PtbB1PtbBCTSKBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9?r~TDATA 9?r~=DATA 9?r~9DATA 9?r~9DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabZFVocYFWO_GATCFeat//YGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~J$LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMF5P1RLF1"P1WDF::P2AMFCRP2BAFFGP2RLF>PBASFF!8PBASFFPCONFF.PCONFF,PDMFTPDMFPLOCF( PLOCF#~PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACAoSPACSPACAoSVER4.0SVER KSVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCHphDYbigphQLcphTRphTRRz0L/kbE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++m}Il4NUu|18$f(wG?*3['B (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i`UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|GB 9th#>rjaxA7+EO< s99OTM$z xMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGPBASFF!8PBASFFPCONFF.PCONFF,PDMFTPDMFPLOCF( PLOCF#~PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACAoSPACSPACAoSVER4.0SVER KSVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCHphDYbigphQLcphTRphTR