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###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 468665b0d4d741379942bb0331aa0644Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-Well$K1336_F7_Ageratina_ligustrina_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z+Nn,BVhw7_|e*ugR3 y]KIVkt`OFB?6%!+&&17<AHR]hs*k/U 'IflQo#[0zU8! 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JISs{b7%:=5Oh[,jno8L8q~X35Cnu Q%akFPn=\?4y]B67j6eE=(=bFe5\Nj;L*KzO7rNVC5SBPvZSFW8sNku2>pg'bv]TPc{~fKV|?TIKTav=&y\HA@j}g|O?dv/qB[F9)pAMCLtZ<=UvYR'sMgtQJ)|TcaU0}jGPQrMCHZe1lwJ{vt/HDAD@W\(U}BWhNFocS;D8rc`EkmOl38<;nJV\F]1oiSc=q;F|Jf16${RIIU;9P\:RS9ZLi(ZnUc'mN|I:z_/ _1`s[b4$c@Xf!IUuhv|4n }=2v{mU%]hp+eCxi uZt<(UN&{..WntDJIH:QICGAAGAAAAAAAGAGTCCTATAACGAAGAAGCAATAGAATATCCATTACCCAATTTGACTTCTTGGACAGAACCACAAAATACCGACATTAAAGGGCGAAATCAAAGTAACCTCCCATCCGGCATTCCGCCTGGCAGAGCCAGGGCTCGCATTATCTGCCAATCCTTTTCCGGGATTTTGAAATATTGTATGGACCGAGGTTCTTACCCACATAGTTTCTTCCAAAGGCCGGTGCCAGGGAATCTCACCTGCCTCCGGGGGGAAATATCAAAAACTTGCTTTAGTAGCCATCCCCTTAGAGTCCTCTTGAAGCGGAAACGCGACTACAGTGCATACTTCCCTAGTAAGGTCTATGGTTGGGTGTTCATCCCTGCACTCCCCTCTTTCTGGTATAATTGGATAATCCACCGGGAGAGTATAAAACTCCTCATCAAGCTTTTCTTCTTGGTCAAGAGGTGATAGCTAATCTTACCAGGTAAGAGGGTTCCCCGGCTGGGGGTTCCGTAGGTTCCCAATTGGGGTGGTCTGCGTTTCAACAACCAAGGGATGCGCTCGTTGGATTGGGTCCCGGTGTTGATTATAATATTCACTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:40:12 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt>ASPt>B1Pt=B1Pt=BCTSKBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9#rFTDATA 9#rFDATA 9#rF9TDATA 9#rFDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE5LAST~~J LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMP5;P1RLP0P1WDP9P2AMPCP2BAPPGP2RLP>{PBASPP kPBASPPPCONPP.KPCONPP+PDMFTPDMFPLOCP'[PLOCP"PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL%%TSPACAlSPACSPACAlSVER4.0SVER KSVER,,/Scal@Scan@TUBEF7Tmpr<UserphARphCHphDYbigphQLcphTRphTR*This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt>ASPt>B1Pt=B1Pt=BCTSKBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9#rFTDATA 9#rFDATA 9#rF9TDATA 9#rFDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabYFVocYFWO_GATCFeat//YGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE5LAST~~J LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMP5;P1RLP0P1WDP9P2AMPCP2BAPPGP2RLP>{PBASPP kPBASPPPCONPP.KPCONPP+PDMFTPDMFPLOCP'[PLOCP"PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL%%TSPACAlSPACSPACAlSVER4.0SVER KSVER,,/Scal@Scan@TUBEF7Tmpr<UserphARphCHphDYbigphQLcphTRphTRY#rNT/$4OY ~?p96UI//`JiOzJ} {&kdA[L25nWsyZKh~8JePS"Fp?(J/Hcz,(,y]18`F7IOo_3@/$9E^E:D+UZ~en?R/FIOb]rDKRm*u!?Xa./l3672_4&blIk(!Y`%]RI1]f\+!@rIwYWgUTHG&/Bc1h67A"yV+L"o&Hmd1I*w&OLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`_dEKW\lWQ7M31pfQ>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W*c3oAa!jv3}H1,Cz#Z|#e1a_WTDr]?'Hrz9Drv4~K3qoI>*?ag5d7m``k:!.tk}Y=52r =&e=.7I 9'kT'_C:j|(5,grOT1 x>eId|NH:nk3RK0&iL(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@Cz6AqdIqN6`aD/S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0SbXW,VE+,[Ol% eIe]Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*PvASPt>AUDThB1Pt=B1Pt=BCTSKBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9#rFTDATA 9#rFDATA 9#rF9TDATA 9#rFDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabYFVocYFWO_GATCFeat//YGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE5LAST~~J LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMP5;P1RLP0P1WDP9P2AMPCP2BAPPGP2RLP>{PBASPP kPBASPPPCONPP.KPCONPP+PDMFTPDMFPLOCP'[PLOCP"PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL%%TSPACAlSPACSPACAlSVER4.0SVER KSVER,,/Scal@Scan@TUBEF7Tmpr<UserphARphCHphDYbigphQLcphTRphTR