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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bcb622162e874a83a7a4751383ccd5bfSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"############### K1322_H2_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z!?e%;Ody .@NUXZ`npO/{T*gO;*  5Nh >u>`~kT<%fF*c@" {wx}(W;n~T!j1(fTEnWD85:I]rv^E0$%3Ic|xP1IrU`U nhzQ5% ""!!%(,0244335;G[xZ;qZ2~Z@.!!&*,-....,)% !#%'&%! 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Mr=M~D'#$  9t)[`3 6}]4e^"KE$ bG;RsM' )g, zj8 $# Cf$D.b2       R x 6E#  H xs#cy+?CGW/      GFo8T: q@(!#&&"    /r9bc5       <w-uo<  !!  %]?+Qa8!   9y/^ aA;P_7   MC>W#XZ+j^3 Ir)L=?L)    "OJaX3N+  /rDB@ JP0V/           +OQ{uH V3  $UECtI.!   6g8ps?}O0  'Xk JFk RV#l"P2 E?\j@)     .Z=7g?& $JVN5{DvK.       =oS|;dM}V?4/,( 8gFFX8#    'J~ Na$rL3$ %I|0XiaC}[D5,&         3^2|;wQ8(  !G2&=.ahL:.$):Oew}n\J;1*&# #^e{.y6    &4HbQ Z}nbYQH>5,$   N?@A.A=LA KB.bcpKB_3730_POP7_BDTv3.mobtermGGGGGTCTGGGGATCAAGCGGGTGTTAGATTATAGACTGACTTATTATACTCCTGACTATCAAGTCAAACCTACTGATACCTTGGCAGCATTCCGAGTAACTCCTCAACCAGGAGTCCCGGCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTCGACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGCCTTGATCGTTATAAAGGAAGATGCTACAACATCGAGCCGGTTGCTGGAGAAGAAAATCAATATATATGCTATGTAGCTTACCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAATATGTTTACTTCAATTGTAGGGAATGTATTTGGCTTCAAAGCCCTTCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTATTGCCTACGTTAAAACTTTCCAAGGACCGCCTCATGGCATCCAAGTTGAGAGAGATAAATTGAACAAGTACGGACGTCCCCTATTGGGATGTACTATTAAACCGAAATTAGGTTTATCCGCTAAGAATTACGGTAGAGCGGTTTATGAATGTCTTCGTGGTGGATTGGAATTTTACGAAAGGGGGTCTGGGGATCAAGCGGGTGTTAGATTATAGACTGACTTATTATACTCCTGACTATCAAGTCAAACCTACTGATACCTTGGCAGCATTCCGAGTAACTCCTCAACCAGGAGTCCCGGCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTCGACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGCCTTGATCGTTATAAAGGAAGATGCTACAACATCGAGCCGGTTGCTGGAGAAGAAAATCAATATATATGCTATGTAGCTTACCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAATATGTTTACTTCAATTGTAGGGAATGTATTTGGCTTCAAAGCCCTTCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTATTGCCTACGTTAAAACTTTCCAAGGACCGCCTCATGGCATCCAAGTTGAGAGAGATAAATTGAACAAGTACGGACGTCCCCTATTGGGATGTACTATTAAACCGAAATTAGGTTTATCCGCTAAGAATTACGGTAGAGCGGTTTATGAATGTCTTCGTGGTGGATTGGAATTTTACGAAA '1;P_mr)7BTo{ &1<JU`kw "/<EP\ht#/:FQ]hu(3?KUbo}&1=HS_lx $1=JU`lx+:DO[gt   - 9 F R ] g s ~    ( 3 ? 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QD LL&ETV]#$X ;A IG%1904;+#^P)=* M^8 b&>?<*G&:< ]\0 2B#35-1 8/C #Z"+"YS@>>ZK.!7EMT= %JH>_aTO#9_?'M\0_MT 21?%::Gb 9D,X PW$ W[*[S3#G.FKI?.3,_;-OTA(<7 W@,.4) P7.N?HL:8.OY>BNZ-, -b;O?&9$9RJ*H-8X #cF1W7N!1/+1Ab/"%,%-0Q +2+N(]#3E(KVL1[_cKY$:Qc,0'UFA E$.DBbKX3 5T/KI1Y@ T!Q-/T1@"Z*6='$K&+@I)DHA\.#/* E4^<]"0 ,5BJA =KIP[DF)F9T53dY647:C-R.X:O+>M)ZY,JOV*8[NC,'aUO+57E=M &X*A2^)9!4T6*UbD6b6:J :MV %IQ\D 'UW'Y5JJOWET&@H #FE0$%1:SV%""c-'/=]F Z@) -/ 2Z ^?tcVh\F%heCiY/l}.}W%!VW @t+(:5}\@Ls$ KKBB1]"FSr#~e?S`v~ *Y/VQW]7W0nF~#aE&nC"a 5>$og\;d< 8f )}QMDiE*;p#.ZP_76z5!{TpuFL!xjFm.ro3bHt71K_.k7P V"a"'@x|<2{'vY&29Yv(;HMzag /L$TsgDS8 vXYCwt -<A]NsK=k'*5 v`XL;{wcx.qYy7r w=%j1 0J`%nH,&|zz~ogd?LQcf@IA/H!BC;45=3,,# !                      8   $&#/.3$%%  ;%8 6!2&!6 %"4!&2-!?$"&97)-''23,&%*./+61/115)&71D<H>;5[-0.>@/*06<7PFH<EKTLH>><=E@fNEEBT=HAC8GKQ9K><Fl7LS+:H#33EE6633336-666p| p\ppmd~8Nd8-H8$-,8Vpf0Ad,;u Hdd,8,!d=,2hF \Z6#{sjq~9W{E~=t~sr? /,^t+vd ZE2xj:RKpT14O:=)UT;s 08f_d]2zLp_lR6QzR,; 8YT8X,j,^p& 3DQpb-|]zp ;WKvs ofY3p+p]pdZ$pp8pd% 4 >KgtNH#uM3Pkp 1H@-$K,%c$I)J!) =&+W E#^;,#/.!=7% . % &2/>)$#6 %$;1 '" *,+$667#;- # ?$ 7  .0('B.5$ 7'-#7"5 &0%*2,I=83$%5):!'(02$"!."0$)14: ! & ("("(%1=1%2! "  T; )0(-(."" "+!-)$#U,< 6&!% ".&3&","&5+<$>!'&!-H<v<mK0(735*!$?' !A-"%$$;4 7 5WJd(( Eg$-'39" '()(. ]51K2SGH,2$H#`{9LIR0<53,99i-N#,KK#$K?8f@T=JQc#Qtz_wgv!' AAAAAGTGTTAATCTCGAATCTGTAACATAATATACACACACATAATATTCTTCACAATAACCAAACGGGTCCGCCCGTTTCGTCACCTTCATTCGAGGTACTTCTGGAACCAGTGTTTCCGAAGGAGGAACAATCTCCGCTAGCTCCTCAGTATCCTCGTATCCCTCGAACTCTTATGTGCACTGCGATTCTCACTACATTCGTTCTCAACAGGGAAGGATAATCCTTCACCTCGCGGCCGGTGGAAGAGGAGGCCAACGGGGTAGGCTCCGAGCCTCACTTTCGATTTTTCACCCAGGAGGCCGGGCCGATTCTTATACCACCTCCTCTGGCGGCATGGGTGGGGGTTTGCTCCTCGTTCCTCTCTCTCGTCAGGAAGAATAACCAAGTATTTCCAGCTTCCGCAACCCCTGCCTTCGCAGTTTTTATTCCAGGGTGGGAAAAGAGTGAGATTGAAACCACGAGCTCAGTCCTTTTGCCCTCATATGCACGAATCCTTAGGTACTTTGCATATTCTATGAGTACCACTGTAGAGCCGAATAAGGATGTCCTCCCTGAACCGTAGTAAAATAACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:21:17 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt=ASPt=B1Pt<B1Pt<BCTSUiBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA :uTDATA :uDATA :u>DATA :uDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~SLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS(P1AMA?P1RLA;P1WDADP2AMAMP2BAAAQP2RLAHPBASAA+NPBASAA) PCONAA8PCONAA6PDMFTPDMF(PLOCA2PLOCA-PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%(SCAN@SMED SMLtSMPL!!TySPACAlSPAC(SPACAlSVER4.0SVER U^SVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCH)phDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt=ASPt=B1Pt<B1Pt<BCTSUiBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA :uTDATA :uDATA :u>DATA :uDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabcFVoccFWO_GATCFeat//cGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~SLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS(P1AMA?P1RLA;P1WDADP2AMAMP2BAAAQP2RLAHPBASAA+NPBASAA) PCONAA8PCONAA6PDMFTPDMF(PLOCA2PLOCA-PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%(SCAN@SMED SMLtSMPL!!TySPACAlSPAC(SPACAlSVER4.0SVER U^SVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCH)phDYbigphQLcphTRphTR<8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0Hi6b>B<\}2b=aL?$>`Oc_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhlh9eD>0\#5+yg2RmqT1*rml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~L3XsHFt|>TP.L!`C%7yclrCk|7[ZIxplfwa:t"@>oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> DATA :uDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabcFVoccFWO_GATCFeat//cGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~SLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS(P1AMA?P1RLA;P1WDADP2AMAMP2BAAAQP2RLAHPBASAA+NPBASAA) PCONAA8PCONAA6PDMFTPDMF(PLOCA2PLOCA-PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%(SCAN@SMED SMLtSMPL!!TySPACAlSPAC(SPACAlSVER4.0SVER U^SVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCH)phDYbigphQLcphTRphTR