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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5154a1751ee946a3a43043edc21942f1Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"###############K1322_F4_Begonia__sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z!*9Qn "(2ASh{zcH) uX; 2Ml /St%)&p_SJB9.pO, ypf^XSQQQOLHB><=@CEED@=963211234430.--/245531016BTm5g6HPOF6wCymd^XTPMJIJMUataq2i5S"Fd36imNbx~mRA70-,,.///.-,+(%"!$$#*?]7 =Z^I[uS:+# !!"""!!! 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(xxRk>Uh& #)(" _|&m7H@Y,J ''"    +' 3z = -2.&   h`Cd@c|:&($     /;pdf#  `9{ J    #nj=!#         ^tX*H %" M]nd# &($   J(1I/j`/cc)'//*%"!""     Q4P>z=       'w{ZtIby7      P*$ O{:        R,4"jC "      G:YW/Z^=aW;S1Y@ _8+*,*#  !       #l\9Z4Oybs)25zG.&%#           \atYKl4      7Sc3V@!    *eDgb8e4      YS\NQyA(#&)'# 7 $klp Wc/VN/%#"     X6 [["LN0$!     ,dJzd!X^MhCtx.E$leA311/)""$$#""!  /s4B_;*%&'$[  ul>)#%'%Q;Q0"Aqs 1tC+#"" <E|$O2&"  P u 4P0"    5x9-P0"   :SsTr/O7>J_9(%(,-+'# IfYf?-'''%#  7y.nU7)$!    :>Wj<"   2q'VoC*    .`#IO4p{l$c 5wzN5*%! 6wBCDP, OkF4.+(! E9DhB.%#!  $X^l?lX8&   /^>K=qF(    #G{'DF+U_^C4+%!)I) EE!k,uRBVAUaG7-%      3c"QgbBlH1# /[JgjS(;wn1Z@.!    'Jz,Ug`BYt5\B1&  @p/`|}d8eF3'   @r-YmhK^C/! (M~!BMB$oN7'  +ODr])[B2(!  1Y[r5{R6&&FxJu8Y?0'"  )UR/    $.=So-et> {oe\TLB8-#  %.6>BDB=6,# y >@kA"AS$ A%FKB.bcpKB_3730_POP7_BDTv3.mobtermGAAGGGGCTGGAATCAAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATCAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAAGTCCTCAACCGGGAGTTCCACCTGAGGAAGCCGGGGCAGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATTACATCGAGCCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTACTGCTTATGTTAAAACTTTTCAAGGCCCTCCTCATGGTATCCAGGTTGAGAGAGATAAATTGAACAAGTATGGCCGCCCCCTATTGGGATGTACTATTAAACCGAAATTGGGATTATCCGCTAAGAATTACGGTAGAGCTGTTTATGAATGTCTACGCGGTGGATTGAAATTTTACTAAACGAAGGGGCTGGAATCAAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATCAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAAGTCCTCAACCGGGAGTTCCACCTGAGGAAGCCGGGGCAGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATTACATCGAGCCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTACTGCTTATGTTAAAACTTTTCAAGGCCCTCCTCATGGTATCCAGGTTGAGAGAGATAAATTGAACAAGTATGGCCGCCCCCTATTGGGATGTACTATTAAACCGAAATTGGGATTATCCGCTAAGAATTACGGTAGAGCTGTTTATGAATGTCTACGCGGTGGATTGAAATTTTACTAAAC ,4:MYhx $1=MXhu|&2@JU`my!-7BO\ir~!+7CNYfr~#/;HVbpz "-8DQ^jv +8DNZgs &1<GS`lz  % 1 = H S _ j v    ( 5 A N Z e q ~  $ 0 < H T ` m y    ( 4 ? K V b m y  " . 9 D P \ h u (3?KWbmy -9DO\hu(4?LXer'3@KXdp{ #0<IU`lx ,8CO[fs(4?JWcp{ %1=GS_kv -9EQ^iv)5BNYfq~'3@MXep} &2>KVcoz "/9ER[it ,4:MYhx $1=MXhu|&2@JU`my!-7BO\ir~!+7CNYfr~#/;HVbpz "-8DQ^jv +8DNZgs &1<GS`lz  % 1 = H S _ j v    ( 5 A N Z e q ~  $ 0 < H T ` m y    ( 4 ? K V b m y  " . 9 D P \ h u (3?KWbmy -9DO\hu(4?LXer'3@KXdp{ #0<IU`lx ,8CO[fs(4?JWcp{ %1=GS_kv -9EQ^iv)5BNYfq~'3@MXep} &2>KVcoz "/9ER[it  % 2% *4>466&)+>6>+/>>>>>>>>1,>;>;;;>>>;1>>>;;>>>66>>;>>>>;>;>>>>>>>;>>;>>>>>>6>;;>>>>>6;>>>>;>>>>;;>66>>>>>>>>>>>>>>>>>>>>>>;>>>16>6>>>>>>>>>>>>;>;>>>>>>>>>;;>>>>>>>>>>>>>;>>>>>>;>>>>>>>>616>;;>>>>>;;>>>>;>>>>>>>>>>>>>>>>>;>>86>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>68>>8>>>>>>>>>>>>>>>>>>>>>>6>>>1>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>8>>>>>>>>>>>>>>>>>.+8>>>8>88. 88>>>>>>>8>>>>>>>>>>>05>>>>:::::6::::6::::6::::::::4:::6*:::::::::::::::1:::::3*::3::::.:(:.:::4::22:26::::1:/::3.15::3:-6661   '!#   % 2% *4>466&)+>6>+/>>>>>>>>1,>;>;;;>>>;1>>>;;>>>66>>;>>>>;>;>>>>>>>;>>;>>>>>>6>;;>>>>>6;>>>>;>>>>;;>66>>>>>>>>>>>>>>>>>>>>>>;>>>16>6>>>>>>>>>>>>;>;>>>>>>>>>;;>>>>>>>>>>>>>;>>>>>>;>>>>>>>>616>;;>>>>>;;>>>>;>>>>>>>>>>>>>>>>>;>>86>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>68>>8>>>>>>>>>>>>>>>>>>>>>>6>>>1>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>8>>>>>>>>>>>>>>>>>.+8>>>8>88. 88>>>>>>>8>>>>>>>>>>>05>>>>:::::6::::6::::6::::::::4:::6*:::::::::::::::1:::::3*::3::::.:(:.:::4::22:26::::1:/::3.15::3:-6661   '!# N:TLK51%_"GA 8+=E2_AH]WZ+^YPOHD8L X9.*E`Q\R 6X,QM@C&RMN #Y'&L0MN(%QYD3*N"6<0FQ15/WD GK"#(D@N GF V' 2..21 !).T&4(M/+C"&)b_b_3%B[`WS"T@<a?,\IE?N_[EA7#Q', IZZ38`'P[J1 )*c;WID  EF%1L.2(3SR23 O =J#',]0 K1S+a8A'5O';/ +<DG. 5Z*]8`b8 Z0R=U^3V.DAAT;6"7BJ ^,?5JW/[#T^5/>FS_A KD9LUPI9>XE L` PX=(H\C Lb_J*9c #,D6^@K0_FX0X*b*0 $?aN!OH*,P(U?:K 'N3O#TG.5(QS%9YS,O@ 12`!ZA)MA[[ O&1^C 'F,N9=JC/a A&AB]8T9>!8O X&RM0$FGF/?J2%8)%$H$Z:\  4X_G]: \]1UE]%2a,[ qFGGDmOgzWY7Z WG-:n F4pCR jG8-3+ VmP!cANt3)t9ML+rZlpPj|9t0]A1M/  S^o%*DG(=gd X!*w%.w;=Qk2:tv5 OD9]wVNVik+#2pLH$Nw 2U/as&|"E%GV0aav~Kd!:Gre}!T H^:Tq _-sR<|}x}eSxaE1Oocq!IN-{}=ld cunT  Ea)/KXdbG\s t(LZ$<YQ :Cma( W f4o)x8R <h0~GN4 2xE&};6H&]Z@oNPK\GI<?6G35:28+%)('%         #             #   #)%(#%  " 1)!'%&+**7',#$43'$,7%('+()2-3&/>,0'+'8.?F0/9*6-26.1779AOJN3hKhBN/>4K2E?qO?TT;@=AHAB]Rs:=DHH:[bO@R37F33755533335?3555prc8pfpp,%nddpGev2']M]y D2Ap8cdB|Lhdwdfv~dLz_t8a:GRRfUh ^d9p4e4i"odOd-, Kg_g%88p_vdU,oNfsp9R2udHcVU-`Gp]yo v82dMptdGpdj]IeZ5d<8B')pp3o8@p HR2p=8ppppVmjB~Svr`1bT!5NB Nwx'3u5_aY?8E(I%j6lLz E*"&`} V2&'.2(.#11(<$ WS/@9""XF ($- 43 2 I+9K,.I4 .%*# +& &" 4&2+B!5!!7&'#*<+&*3 "3 2-:-0(GD4"70549+ -#%($" ,!"6'$-&,&($9%%$'(3$&#%   3;*59+' .'#%*:5/+#!5,#*'"+"$'.0|!$ )""G2#?'$, & +,0L`,2I #'"- $!$.# *'##8+C&3-?2@3!1&/% 0%&!D*>&-j24&?>>"7)7D)@ "2+F,.62+=%2dS03RrROA>ANW00Ms/c$]cQ5" N4AGGATTTTGTATTCTCCAGGTTGTGATGATAATATTTAGACACATAATATACTTCCGTATAACCGAACCGATAGTGCGGTATCGTTGCCGCCCTTCGAGTGTGTTCTCCGGCCCGAGCTTCATCCGAAGGAGGCCTCGCCTCTATATCCTCAGTATCCTCCACTAGTACAAAGACCACTGTGTAGAGCGAAACTCCCAACACTCGTGATCGACACGGAAGGAGAATCCACTACCTCGAGGCCGGTGGTAGAGGAGGCTAACGATATAGCGCCGAAGGAGCCTATTTCCGAGTTCCCCAAGGAGGAAGGTCCGATAACGAGATGACTTCCTTCGGTGAAAGGGAAATAGGTTTGCTCGACGTTCCTCGCTCTCTTCCTGTAGAATAAAATAGCATTTCCTCCGCCGATGATTGGTCAACTCGACGTTCTTCTTCTAGTATTCAAGGTGAGAGATATGTAAAGCACGAGTACCGGCGTTTTCCCCCCATATGTACTAATGCATAGGTACTTTTAATATTCGATGAGTAATACTGTAGAGCTGAATAAGTATGTCTACTCTGTACCGTGGTAAAACAATTCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:21:31 -04:00AEPt@=AEPt@=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?\TDATA ?\DATA ?\SmDATA ?\DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}YLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR-P1AMChP1RLCdxP1WDCmP2AMCvP2BACC{P2RLCr PBASCCTPBASCCR`PCONCCb5PCONCC_PDMFTPDMFRDPLOCC[lPLOCCVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%R%SCAN@SMED SMLtSMPLTySPACAfSPACR=SPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHR[phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@=AEPt@=APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?\TDATA ?\DATA ?\SmDATA ?\DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab=FVoc,FWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}YLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR-P1AMChP1RLCdxP1WDCmP2AMCvP2BACC{P2RLCr PBASCCTPBASCCR`PCONCCb5PCONCC_PDMFTPDMFRDPLOCC[lPLOCCVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%R%SCAN@SMED SMLtSMPLTySPACAfSPACR=SPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHR[phDYbigphQLcphTRphTR6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGxNMVp[12O1MvAQ0HAV=yTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"H+(QF.R8{>Z#oaW&jEp|9-$pVWMOHu+wGkSOj!Q4X+*flVinJils>:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*t> N(1M J.S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0