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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f84c15aa6a244e37a8d9e3b7ecef743dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"############### K1322_E8_Bletia__neglecta_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z #Eq Ce,Mo  a@#f?nYKEDHMRYbq8Z{-2.#ubL3~iVD3  vqnnonkgb_^`dilljgc^YUPLIHGHIJJJJKKKLMPVaqN<q`'f'|h\TOMNRX`ky)pLMzvGo/ BcFG X--$tuS^}U:) !$%$""%'((()+-./03;MkWLgm\5n%rK/#(-159<<94,%    "$%%#! 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Z$%!`*    &d- TA     /s:*x>  = 90a?kW AGu_+P:f:'%(( ?P6|>    *jATQ<q6      Am!5FuBsr=    (\Mw.I   Smw{vl7  )c q 8p9 "Ti&|yCfN#h6 $[*7ZR+     $TCN9 U,>M_}Z>W+       E~ EjqX&f:    6r*9M*  6hz*M?CCocj%| m}O3#      "M^zGyE#   :sYahY |K,      7e3nt>7|U V8(! 1T3XeW2Y8$   'Hv&RjhMsI-  +U$go3wN2    /Y*AA*lG-        (L NvAW7# =qVm(oM8+#(6GZkyudO;)    4w2|(n-       "&,5E_7~"2(Frid_XNC6*    *6BMUZ\ZVOF<1&  @AMjAIA@KB.bcpKB_3730_POP7_BDTv3.mobtermGAGCGATTGGGATTAAGCTGGTGTTAAGATTACAAATTGACTTATTATACTCCTGACTACGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCGCCTGAAGAAGCGGGGGCTGCGGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTGTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGACGATGCTACCACATCGAGGTCGTTGTTGGGGAGGAAAATCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGTTTCAAAGCCCTGCGAGCTCTACGTCTGGAAGATCTGCGAATCCCCACTTCTTATTCCAAAACTTTCCAAGGTCCGCCTCATGGCATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCCCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAAAACTACGGTAGAGCAGTTTATGAATGTCTACGGGGTGGATTGGAATTTATACATACAGAGCGATTGGGATTAAGCTGGTGTTAAGATTACAAATTGACTTATTATACTCCTGACTACGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCGCCTGAAGAAGCGGGGGCTGCGGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTGTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGACGATGCTACCACATCGAGGTCGTTGTTGGGGAGGAAAATCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGTTTCAAAGCCCTGCGAGCTCTACGTCTGGAAGATCTGCGAATCCCCACTTCTTATTCCAAAACTTTCCAAGGTCCGCCTCATGGCATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCCCTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAAAACTACGGTAGAGCAGTTTATGAATGTCTACGGGGTGGATTGGAATTTATACATACA#,8KV_ir~*7CR_nz+6DNZeq}(4=IUboy '1=IT_lx*5AN\hv(3>KXdp}&3@KVbn{ .9DO[ht   ! + 8 D O Z f q }    % 0 < H U b l y    , 7 C P [ g t    ' 1 = H T ` k w    + 7 B M Y f r ~ %1=HT`kw*6BNYer~&2>IVbo| $0=HTaly  ,9EQ^ju)5?JVbnz #0<GR_kx,7DNZfr}'3@LXdp| $0<IT`lx !,8ER^ju*6BNZgs~'3>JV`my  #,8KV_ir~*7CR_nz+6DNZeq}(4=IUboy '1=IT_lx*5AN\hv(3>KXdp}&3@KVbn{ .9DO[ht   ! + 8 D O Z f q }    % 0 < H U b l y    , 7 C P [ g t    ' 1 = H T ` k w    + 7 B M Y f r ~ %1=HT`kw*6BNYer~&2>IVbo| $0=HTaly  ,9EQ^ju)5?JVbnz #0<GR_kx,7DNZfr}'3@LXdp| $0<IT`lx !,8ER^ju*6BNZgs~'3>JV`my    :2..1! )2$*>>>>)'>>>1/>8>>>+>>>>/;>>;>>>;1>>>;1>;>66>;;>>>>;>>>>>>>>>>>>;>>;>>>>>>>>>>>>;;>>>>*>>>;;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>6>>>6>;>>>;>>>>>>;>>>>>>>>>>>;;>>;>>>;;>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>*1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>1+13>>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>>8>>>8>838 888>>>>>>>8>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>:.",6:6:::6/:::::::+:::::6::::::::6.:::640664::::::::4::43:/::1::::6::::::::+   '#0.,  :2..1! )2$*>>>>)'>>>1/>8>>>+>>>>/;>>;>>>;1>>>;1>;>66>;;>>>>;>>>>>>>>>>>>;>>;>>>>>>>>>>>>;;>>>>*>>>;;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>6>>>6>;>>>;>>>>>>;>>>>>>>>>>>;;>>;>>>;;>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>*1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>1+13>>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>>8>>>8>838 888>>>>>>>8>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>:.",6:6:::6/:::::::+:::::6::::::::6.:::640664::::::::4::43:/::1::::6::::::::+   '#0.,6%^JK/BS`?]A [ 2SM9:0ZP(3#5I<N(2[%6%_ $]>(SU>!=5 >&52SDI]2 ;'2 A8>N/5=.J7 G`6=X.DTKH73"0D@\Y1>^M53?8%='1$(R=,];EHJCK-FRK1VH=*J:/]9&8>2-/N*DJ3/) Q).&!7 -3CP7bD1*a=&>WP4!56K.';@PNVV^R.I3KJa,RV/,*5 4I^LIG2B,KR'ZPC)?I5,6VI*?:]c/SU(#0Y-&@+&#!F-</" ((94 2@^^(IG%ZW+VDGGR@5<C9;0?C$V!9:^bE91;O?<aEE%<#(,E=A:,$*$.@?G 1I *EKV9L9LJEWC<?Y(%a8 U%>.)<+A>$T>K M*:$$@W;(`6`?P.73N,<.-#Q?cOS 7%cE40+FOW -Aa;B&Z#S%.I5?F5A=0KD#Ca%"bRJDIL.PcJ,!9:9X`68RZ b2aZ_aL%@ / M lCAh%v 7Mp rsMev7l1wh.uJKk,e|B]P)B"gu$k&OME-!#R1u3;zPvam` 6 \& B\J,2b2Kw&=!i>XRiF"KHtXU`D l_Ot:(sb4ITM*vPhjM'c&*P[=0Y>O.u,]BCH~elJ:,;hH $Mv #}y3A&b*4x\n=HTZ#9\W/*Cx]&BNEG1%3YA  ? uS7hdK=GWVj yHiU2^ jsYL q0} a% . 2pM{X)W ' ? Te V fR'CG{i%]S@BNCJDE@=B9<6:/1,/.#&                 ' #-    3 &     +,"$*%+,&( ).$&"++*;0?8A!++,6:9.VI1M+_&$(45::B.A47; F("7!0$ ""**))""")")#)"))p1q,~{9pdp'xP ",F17pm,8i}@,C8v,,ZsM'ilO}9Lz:Bjdd G'dd,UdG*G6r}~+i0d~p2Le$16\?Nl -~vD"dD08$B p4 t9dddKhuTpd-h)Q[GddC,d!,Dd,!vPd|I+"dqopI.]a6h e5&MZc~ 5Em~VgsTZp?8NHzRpb<PI V.8As"2#FHrO J55P(d{X(ZU"KYW" 3%3W@P&/&-%F/>&-2>R,8!6 """1)$&.* !)" 92!)>"4/AU142*"""+$*)"%'&4'/#*!! % !#" $0#(&#4+,)%1" .'*.!% /( 1% -3<A# &"&$:&&$!# )0""&)0+*/-%""'!$!*-2)$-3*'--*!&2!$!+(#0)+ $92-T& ,,*$(1+E336 2%6"5/-/$A 3&*%7<)/'&B0!.&D]W!u]*#"6%!1''#H'O#)!')&- .2=@Ej6,!7;#6"&, '46!17!2^R,H% $FB*1"!';<U$2939@>e+NB=DGEV][e4^H9>Qlh0fYo|jAGAACTGGTTTTAATTCAATTGTGACGATAATACCTACCCACATAATATGCGGCCGAATGACGCAACCGATACACCCGTATCAATGGCGTAATTCGAGGGACTTCTCCAACCCGAACTTCTTCAGGAGGAACAAACGCCGCAAGAACCTTCGTATCCTCCTCAAAGTCAAAGACTACTGTGCAGGCAGGTTGTCCCAAGAATCGTGATCGACACGGAAGGAGATTCTTTCACATCGACGGCGGTGGTTAAGAAGGGCAAGGAGATAGCGCAGGTAGCTCACATTCAAGATTTCCCCCAGGAGGAAGCTCTGATTAGGTGATAAACTCCTTTGGTGAAAGGGGTATAGGTAGGATCGGCTTTCCGCGCTCTCTTCATAAAGGAGATACGAGTATTTTCTCCTAAGAATACGGCTAAATTCGAAGTTTTTCTTGCAAGATTGGTAGAGGGAGAGACGGAAAGCAGGAAGGCCGTCCTTTTCCCCCCAGATACTAGAATTCTTCGGTACCTTTAATATTCTTCGCCCTACTCTAGAGAGCAGCATAAGTATGTATTCCCCGCATCGTAGTAAATACACACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:22:02 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?ZTDATA ?ZDATA ?ZSnDATA ?ZDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE8LAST~~}VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR*P1AMChP1RLCduP1WDCmP2AMCvP2BACC{P2RLCrPBASCCTPBASCCR]PCONCCb2PCONCC_PDMFTPDMFRAPLOCC[iPLOCCVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%R"SCAN@SMED SMLtSMPL!!TySPACAfMSPACR:SPACAfMSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE8Tmpr<UserphARphCHRXphDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?ZTDATA ?ZDATA ?ZSnDATA ?ZDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab:FVoc)FWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE8LAST~~}VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR*P1AMChP1RLCduP1WDCmP2AMCvP2BACC{P2RLCrPBASCCTPBASCCR]PCONCCb2PCONCC_PDMFTPDMFRAPLOCC[iPLOCCVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%R"SCAN@SMED SMLtSMPL!!TySPACAfMSPACR:SPACAfMSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE8Tmpr<UserphARphCHRXphDYbigphQLcphTRphTRU[lWQXbkOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt4OuYt$,64WG;~uy@HR_8;%dZ3nuo{D'`,$AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[ALYZB "Te{:(tR[vdAgf+wq,vg>}Zjp9`DH8hP3xA)O5acMYU/G+G)@-mq5FaT%ycX}OuK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;