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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d8f409f39cc94949a1deeec2fca78327Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################K1321_F8_Acalypha_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zTSSSRRRRPLHB=96443333467876421/.-,+*+,.000.-,,-/00.,)('),058:;;;:;=?BFHIJJJMRZdoy  3G^x%0<IWeswS+yL!taTJC=82,&"!$'*,,+*('%#!!!"$')+,+)'%$$&(*,----,--.01356789;<==<;<@HR`pVM|r:p1jXKC=9778:>ADDB?<:;=ACDB?:51.,+*'$ $),.-*&    (5CUm+GWYN8^B.""/Fj1X8G1V3gr39zR6""'+.120,&!$4Nt \t>Y7!   !$%"   .Kx?QsJB,'MC       #F|x7N$         %.Bd/{RwL4++.368:?Mlq]l1J?Tb]FiGXd  !XLV*|6 +6@GKIB8+        .>LVZVL>0#                k8qGU7cl!Vp+"(/6;:4+ !&,045530,)'$""%*//'k-QPjB/:9+=gk-R%L Vx?sJs'%,%YjKaRqHtQbT\2'.,'$&,4;ACB>8/' %*.0.)#!"  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K V b o |    * 5 A N Z g s ~  # . : E Q \ i v (4?KWcp| $0<HUamy+6CO\ht'2>IUco{ ".;FR^iu(5BNZfr~'1>IValy!.;HS^jv*5AMZfs~'4?KWbnz !-9ER^kv +7DN[gr~&2<FP[ht#,     1*-$55:( :4":#>>>//>&!>/8>>/1>>83>>>>>>>;811>;;>;;;;>>>>>>>>;+>>>;>>>>>>>>;>;>>>;>;>>>>6>;;;>>>>>>>>>;>;>>>>>>>>>>>>>>>>>>>>>61!;>>;>>>>>>>>>>>>;>>>>>>>>>;>>>>6>>>>>>;>>>*>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>8>>863>>>>>>>>>>>>>>>8>>>>8>>>>>>>>>>>>>>>8+>8>3>>>>>>>>>>>>>>>>>>>>>>>>>>6+>664>>>>6>>>>>>>>>>>>>>>:6::6:::0:::::6::6:::::::::::::::::::6::66:::::::0::*::::::::::::1::::63          1*-$55:( :4":#>>>//>&!>/8>>/1>>83>>>>>>>;811>;;>;;;;>>>>>>>>;+>>>;>>>>>>>>;>;>>>;>;>>>>6>;;;>>>>>>>>>;>;>>>>>>>>>>>>>>>>>>>>>61!;>>;>>>>>>>>>>>>;>>>>>>>>>;>>>>6>>>>>>;>>>*>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>8>>863>>>>>>>>>>>>>>>8>>>>8>>>>>>>>>>>>>>>8+>8>3>>>>>>>>>>>>>>>>>>>>>>>>>>6+>664>>>>6>>>>>>>>>>>>>>>:6::6:::0:::::6::6:::::::::::::::::::6::66:::::::0::*::::::::::::1::::63     - Ba9^1*0R)TPDB.6 <a,81<-G`VI7 $)* (6S**`X7FE2[<>1'_)W DW,*.W9>/22GN@?Ub=+XA//XH;XX`1"_;;KR*:2C&N/)<RGO+$6T6%b%=5S 0"K5)?cK</PKJN$6;R>P*;A*,`"S/J=B1G*D<;(,TYU^'%>+HXF<N* 2W611a2F`FU+-c; ZR<NG1CK@,A4^ab MK.a5HI5?G[0/M<K,H*b721<F19Z/>I'= R)C!bVbOP,.XD;6=a!$86 _4V$=`8_MU $#I(9W',BM%,J2$D$GX7G=<1"#DV E9"H4`"&.,d$-BT&E%[?( -2$I-@D;5'G;"ZZ OF& `bQ^?@U^:U#!YSb=F P)$))W1 X> 8Mc>ILOdH#IS /L"LEJb2R3;D>&ZU/cUAO*.S ,!5F3/MbO <6E`` \C>GV2;_'=SNQSH<IBZTRa(3w_ sZ XWFLOBadYl> ~ijSU5#E.W)\pbM0F]$(_%2/ uk9#mCudNRW_:WxnxSXN0,>rb#)V>W-LU9) s"U I0N+P#")V~t d97 *j=\ N0-f.Pd?{S<K &s+(bn:>B:i=FaK~& D. S I*Me{7k'vUd -{riYduKv| #g[S(}!\+IEO*I4- #2*Ft 1e.] >/mJJo6v_qz>BJxWeQQ'X'K!&F]g Y DS/* >SR 0 H0O#;Ac$urxzl^WOBijH;=GG=.C-+1%" 5 )          &   (         '  #%$. )!->!7"( 2%4 .%:#+&&'(5,6# (0,;##&*8=.84@2>&+/3B)9?"454D"#16!#6V)2#-.''''''&6&&&&&&pSHys|k"ed[.LN1JWdu*uM`vF +E/#EF0*e"XdOT84,a\a M;+InZ,|dV,n]~\ #Bwwv#*(dV,r(6d8MCDWzIAtvL/Z.!>3d+w.glwtpp31L sm ,|v1Kd48z?Ip]JDD`RDd&wd,pl 8)U`-phJ6Mk OpD2mRKHRG9r6bG`!_,9 .B<m, ?&*E%X#C<G)OHD"' /&4 !+7,*"()2%(  6)! )3%C&"1;'; $ 1='%#   $9 % # A/ ! !&*20 / ($*&'& '0"!#(!1#$"5O$Q,3!%*($&$)8(/%"(+.!81$ ")88)#&J0(.;: 8)52 ,,-'"$+#$*!,1*&=(+%-B00%!?!Q6#&& *7,$,7"* !33&315"6 #"3Gu82-&@:=@3+%!?13$;8'" -/?40+0"&I2$& CD$:, >(& H*4#-""$! %;!%) <3L)+"+!("?<7<.$'F;00*"=5.*55$.gY8=0oaN5 @[AAAAATTTACCGTATAGCATACTCATAGCATATACAGACTAAATTTGGATTAGTACACCATCCCCGAGAAAGCGGACACTCGGGCACCAAACCTTTCTCACGCCAAAGACGGAGAGCCGCCGGGCGAAGGGAAAAATGACAGGAGGGGGTAAGGCTCTCGTATTCTACAGAAAGAAAGGGCGGACAGACGAGTTAACCTACTTAATTTTCTCTGAAGGACCTTTCGTGGGTATCGGAGCTTCCTCCTGGGGGAAGTATGGAAAGGGCGCCTCAGGAGCTATATCGTTAAACTTCTCTTCAGGGGAACGCGCGAAAGAAGAGCATCCTTCCAGAGGGAGAACGAGAGTTGGGATTTCAGCCCTTCACGGACAAATTCGAGGAGGAATAGGAGAATGAGGAGGCTTAGGATATTCCTGCTTAGGATTTTTTTCTTGAAGCCGAAGAGATAATCAGTAATCCGACGAGATATGAACATCCCCTGCGAGGGTGTGCAGAATGGCATGAGTCGAAATATTATCCATCACATCATTGGAGGATTCCACAAAAGAAAGAATCGGCGGGTTTATTACATAACTTACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:34:29 -04:00AEPt@DAEPt@DAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?TTDATA ?TDATA ?TS5DATA ?T҉DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMChP1RLCd0P1WDCm<P2AMCvHP2BACCzP2RLCqPBASCCT[PBASCCRPCONCCaPCONCC_PDMFTmPDMFQPLOCC[$PLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAd.SPACQSPACAd.SVER4.0SVER ~SVER,,/Scal@Scan@TUBEF8Tmpr<UserphARphCHRphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@DAEPt@DAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?TTDATA ?TDATA ?TS5DATA ?T҉DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMChP1RLCd0P1WDCm<P2AMCvHP2BACCzP2RLCqPBASCCT[PBASCCRPCONCCaPCONCC_PDMFTmPDMFQPLOCC[$PLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAd.SPACQSPACAd.SVER4.0SVER ~SVER,,/Scal@Scan@TUBEF8Tmpr<UserphARphCHRphDYbigphQLcphTRphTR]%A'on!w.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwN1d^M?92ZsM TWKr:luo/qc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqiH(IM/C{&wu6 olG }I9jsG,XQ9P9$M8`+MTk0WC.%o,n`!3CS[jWcgnW6Ob#7!VkWtt/0q$1jf:FTt)+'4|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_S)&!n_f,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"