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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2368423d5e1b48bc972b0c5e89ed68c3Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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HIc4d8FS'dC>a~3d8 ypzepWpp2 D@#rR VA[rMF6Gar%(X ;|!QY3;BEez 3 e9q/ 7+Y$; 1 " %& "2H0#$!!)&%'"  $)*,B<0'# !  $&.( )0'$)"%0%2+(")6()*2.4*"!'%  ,"#)!-4(6%!!!*&%'("%, (& !,$%4,,2!..,+'#1:$5%( (3 ,)42'$/7&&%&*)0" "#=0-*:53TvJ4 (*) !=  '+#060& -H <!G3$->A ,  4[A!% #'#(*.?.T%66!;=G8O/0:@0(LiCQT(h'sT.GAGATTTGTTTTAATGCGGTTGTGATAATAATATGTAGACACATAATACATGGCAGAACAACCCAACCAATACTCTTATATCGTCACCTCCATTCGAATTTCTTCTCCGGGCTTTGCTACATAAGGAGGAGGAATCGCCGCTAGCTCCGCAGTATCCTCTTGATGGGCAAAGACCAGCGGGTAGAGAGGAAGACACAACCCTCGTGATCCACACGGAAGGAGAATCTATTTCCTCGCGGGCGCTTGCAAAGAAGCAGAACTATATATCTGATATATATCCTCTACCATACTTCCAAAAGGAGAATGGTCTGATAACGACAAGAACTCCTACAGTGAAAGGGAAGAGGGTTGAATCCCCTTTCCTCTCTCTCCTAGAATAGAATATACAACCAATTTTTCCAAATAATTGGGTTAAATTCGCAGGTTTTCTTTCTGGATTCGAGGTGGGAAAGATTAGATTTACGAGGACCGTCCTTCCCGAGTCCGAAATTCCAATGTAGCCGGAGTTTTAATATTCGCCCGGTAATTCTAGAGAGCGTGATAAGAGTGTATTCTCCGTACCGTGTAATAACAAATACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:32 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt'ASPt'B1Pt&B1Pt&BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >} TDATA >} DATA >} NDATA >} DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~t LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISHP1AMB_P1RLB[7P1WDBd?P2AMBmGP2BABBqP2RLBhPBASBBKiPBASBBI'PCONBBXPCONBBVPDMFTPDMFI PLOCBR/PLOCBMPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%HSCAN@SMED SMLtSMPL!!TSPACAgSPACISPACAgSVER4.0SVER uSVER,,/Scal@Scan@TUBED2Tmpr<UserphARphCHI"phDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt'ASPt'B1Pt&B1Pt&BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >} TDATA >} DATA >} NDATA >} DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~t LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISHP1AMB_P1RLB[7P1WDBd?P2AMBmGP2BABBqP2RLBhPBASBBKiPBASBBI'PCONBBXPCONBBVPDMFTPDMFI PLOCBR/PLOCBMPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%HSCAN@SMED SMLtSMPL!!TSPACAgSPACISPACAgSVER4.0SVER uSVER,,/Scal@Scan@TUBED2Tmpr<UserphARphCHI"phDYbigphQLcphTRphTR0'"sIR*:"$JQ`Q5f|{ce+dr6>svpkx5yu(p4-dId_$_;zay@Q>ko2Uz| Q-M2"ZwclQ[vL}W$620e:jA:>y7LIG!Hgn+1Ab;*5" ~Jz!#fn2+Xb[l"G>H1,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM{3!dX!%~Afzv*Cy KQI@'.%Nz7R@X`@kjJ}B~xGH$i*8j|]]u.ibu:,n_SUgi8i(=l27&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/Ygn :E-14db*w.2{Fo!?OqH~|g8SD'aum(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT9zH'zeE^O{U,\)lOY%}WeoVL6I-In(c:5+(,8G:uLB\(CCW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1nK-k]|sOJr4{6QuA!9@1%ilJG82G [7P^E$62:gt4]u<9$He3e&KO%x'R2_$2!dZ3nuo{D+`($AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt'ASPt'AUDTB1Pt&B1Pt&BCTSuBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA >} TDATA >} DATA >} NDATA >} DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~t LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISHP1AMB_P1RLB[7P1WDBd?P2AMBmGP2BABBqP2RLBhPBASBBKiPBASBBI'PCONBBXPCONBBVPDMFTPDMFI PLOCBR/PLOCBMPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%HSCAN@SMED SMLtSMPL!!TSPACAgSPACISPACAgSVER4.0SVER uSVER,,/Scal@Scan@TUBED2Tmpr<UserphARphCHI"phDYbigphQLcphTRphTR