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(((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9af3bf97f3ce41b7bba375aa389d1882Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"##########K1321_B3_Solanum_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z#G_mt} 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39,tC qoc$L%m^pC^X!tIsgiP'0.56Qk%N@vIj DT4{5sTaSsBWS L4y&6A{U7ntH`qGhQZM)nt`7:Mni~L^8i.o+z,MJrQ~=e|_[.  ,bJ\ZaU%T!Y3wf1_8 o?f2^6 Xasiulz mf^42;uI''Mfc]a_%!G/icu;$bT6zi k /E8 hv H bOhf(1I->};-\Ze#FOO+=H1KE3;=(:H, &%"              $         6     % )&$6  +%!,**1#!+1*(9&#2%'+%"'#2947#%,&+'%3$!% (U&,  $$2zppZp,px8e/Y{D%pua]G{WzNOfw[)[c]nG0EgJiwW]=wHGzA\8!7Z%AB,IReRC+{_\sdO70iPMh?v-9Xy`{X0`pb83,"dWv{-W &m* \`p6xNpX?\L-D,o\W0T*cy?-0Y8T rIF,p=ppnVpx\ {SJR]M`QVd?l3tKGpPSo./mq/ C >QQ$@7GFx>a @N3]8i>6`EB,C ^3IX5<,l) F'@4TI?4+3,%3"@2A"Q#EJT$)2-!J."$]:p86.*@ fs"'2"=@% +N.X#*T/-]DX{#Sz9L)).E#%&A C:+2"-/+@)' <(Q4GB,s<:4&Q&( G $/14C/o(%>#-&J "@D3$N;0)4$1 'F B+=2//05:=!,*$$"B.'Y':0'A#&? 4%D&&)11!&$M#;=!4 3"% B$&,#=N,"U-!QO#Z>u)C:1*. h<#- <U86;L06#5,!+*F*  #O=<$80.%It+d-!05>6H ($ E##+9 .1:-<=5OH<*"+j7('>1%. & '$*)U]8@/ 6KJ27/++-0J3LK"5$F,"2}$dEUBT-HHM#: z K]I;I)Y+AAATTGGGTTATATCGAGGTTGTGATGAGAGCACCTAGACACCTAATATACTGCCGAACAACCGAACCAATATACAGATATCCTTGCCGCACTTCGAGTTACTTCTCCATCCCGAGCTTCTTCCGGAGGAACAAATGTCGCAGTATCTGTCGTATCCTCTACTAGTTCATAGACCACTGTGTAGAGCGATAGACCTATCACTCCTGATCGATACGGAAAGCGAAGCTTTCGCATCGAGCCCGGTCCTAGAGGAGGAGATCCATATAATGCCTATGTAGCCCATTCCCTAGATCACCCTGGAGGAAGGTTCGATAACCACATGAATACCTTCAGTGGAAGTGAAGTAGGTTTGGTCCGAGTTCCGCGCGCTCTACCTCAAGGAGATCTGAGGATTTCTTCCGCACATTATTCCTCACTTCGAAGTTCTTCTTCCCAGATTCGAGGTGGGAGAGATTGACTGGACGAGGACCGTCCTTTTCCGCCTAGAAATTACAATTCTTCCGGACAATTGGTATCCTCCTCCCACCTCCATAGAACCCAACACGGGCGTCCGCGCCGAACTTGTAGTATTAACAACCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:51 -04:00AEPt@|AEPt@|APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XSnDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}RLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR&P1AMChP1RLCdqP1WDCm}P2AMCvP2BACC{P2RLCrPBASCCTPBASCCRYPCONCCb.PCONCC_PDMFTPDMFR=PLOCC[ePLOCCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPLTSPACAeeSPACR6SPACAeeSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB3Tmpr<UserphARphCHRTphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@|AEPt@|APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XSnDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab6FVoc%FWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}RLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR&P1AMChP1RLCdqP1WDCm}P2AMCvP2BACC{P2RLCrPBASCCTPBASCCRYPCONCCb.PCONCC_PDMFTPDMFR=PLOCC[ePLOCCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPLTSPACAeeSPACR6SPACAeeSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB3Tmpr<UserphARphCHRTphDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gt4]uL~$L8q"7u2upB0L)8.HA#Y]A~5|vroW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#t[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}nz:z5>vQSdW79ZYdj8WoQK5F1_;T,V2%4oNYkk?[g23491B@naFJ4\)hsFk%|LO%~ kv*PK@|Q$OEHbX\"B-8'i2{&[y3( ]'ngg61C8dk7YZFU?-4$&LkfG;7oF`|zvn[Y<,h`+m&wE6Gz zF/"Wtixy$|FRrtlqaxkabCDk$OE-`{siO}L6iyZnsz3P<}u^ZqNX+9_e V&&OE6OLo X:QJf9/qwAxew>6?|$lQ%:XGTtrWV*AEPt@|AEPt@|APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTIB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?XTDATA ?XDATA ?XSnDATA ?XDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab6FVoc%FWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}RLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR&P1AMChP1RLCdqP1WDCm}P2AMCvP2BACC{P2RLCrPBASCCTPBASCCRYPCONCCb.PCONCC_PDMFTPDMFR=PLOCC[ePLOCCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPLTSPACAeeSPACR6SPACAeeSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB3Tmpr<UserphARphCHRTphDYbigphQLcphTRphTR