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  $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 27360a754e76444c9a3256e0db8e0177Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-WellK1320_H11_Myrsine_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z&1:?AABGOZfr}vnhgggcZL;*|z})4ASl7Qj{fN3{V4xspkcYMC;520/-*&#! 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"$4 * "& "!1  $(#%/#*!%,)-+)082'C)+.#!&'70&1/5@5<,. 1/</17+/"=0,71(*"24/?40959?;C-D.;*A&+5?@F=/5=>@EN58B8E/K626E'1BK5G5:IBANMJAOKTBDZBUMELH/9S^HFNUO_ENG^aVT\IG_P`KUJXWdXOhgQN[T^dsm[WTTNc]cx[Sc|XZqpvSgLWbmorSrjic[^Vcdg}}upsnyn|rff|wwxlxwwxqv `jtp`ppppssspsfwfpffffjDfCp5?uCz4Iq,pq"Ah0Y8qF8,p7Q.Sa'dB8I^pdnf\;@JU~!%DyJ6%p8Sqy&Bpp5T;2i<{,#]p+20/p I}-8p/upa/p^v8~pZ:pN#ppgRda pdfL <:{+h+80eGyBBz:4ZikJs8xt-7(3@}][CIG47iBgM.PJ#K1__tqD@+ gS,:41&#% 'D ,.4 $)o.L,4 :[)#!'$$9E/)<0%!++3$C%CO & #+#,* %- $&)!)/4 6!$,G!#(13,+7$"13% 7V $0!, +"%>,W(0/)()A-"#)U/37A3(!#C0(% /50;D0%2*'3GG***#-''4=8$&3M@:]30?&(H.*.9'D C-0#;?*6=<=&%<?!29<hD(7Y*\{9.14*DZ"kH5?`+/:$9f3NN9^LFEGN=C2a3xCWBMJ)~I26E-8F/;/H%(0*IGgWH=W&XUKw0rm6sU?n,/WXX-QT.9yZ]OD9 [BGye[$rk7G X.W_|J_CAAAAATTCCATCTATACAGAGCAATCGAATATCAATACCGGATATGAAGGGCCCCAGCGTACGACCCATCACCATCATGGCGGCTCTGGCCCTGGCAGACGGGGCCTATAGGGCGCCTCTCGGAAAAGGGAGGAATGGCGTAAGGTCTTTATAATCTCAGAAAAAACAGACAGAGAGCGCGGATCGACCCCCTTTCGGCTTCCCTTTTGGAGAGAGGCGGGTACGCCAGCAGGACTGCTTCCGCAAGGGGGAAGGCTTGACTAGGCGCCTCAGTAGCCAGTTCCTTAAACTCCTCTTCAGGCTGCTTCTTGAACCAAGTTCATCTTTCCATAGGTAGGTGGAAAATTGGGACTTCATCCCTTCTGTCCGCTATTCAAACAACAAGAGGATAATCCGGATACTCCGGATTTTGCTGCTCCTCCGTCTTTTCTTCATGGCGAAGAGACAATAAGTAAGCCGACGATATATCAATATTCGTTGACACACACTGAAGAATATTACGAGTCTAGGTATTATCCATCACAACATCCGTGTATTCTACATCCTCGATACTCTCTGAAGTATGACATAATCCAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:39:08 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtpASPtpB1PtoB1PtoBCTSKGBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 9=rzTDATA 9=rz/DATA 9=rz9DATA 9=rz#DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~ILIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB5lP1RLB0P1WDB9P2AMBBP2BABBG|P2RLB>tPBASBB!PBASBBPCONBB.PCONBB,dPDMFTPDMFPLOCB'PLOCB#\PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPLTySPACAl SPACSPACAl SVER4.0SVER K<SVER,,/Scal@Scan@TUBEH11Tmpr<UserphARphCHphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtpASPtpB1PtoB1PtoBCTSKGBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 9=rzTDATA 9=rz/DATA 9=rz9DATA 9=rz#DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabYFVocYFWO_GATCFeat//YbGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~ILIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB5lP1RLB0P1WDB9P2AMBBP2BABBG|P2RLB>tPBASBB!PBASBBPCONBB.PCONBB,dPDMFTPDMFPLOCB'PLOCB#\PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPLTySPACAl SPACSPACAl SVER4.0SVER K<SVER,,/Scal@Scan@TUBEH11Tmpr<UserphARphCHphDYbigphQLcphTRphTRTrU]wfgM6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! P8X-o_}4]'G' YHT+(aQQxnJilXSXW .C=vz^`%C];p3TVu>g,\9K:qmksG6\H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9ni|\Z&qNl*]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9UZm0DlBFv"Z66`,9SPQP#8\4d+=Ht+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!tPBASBB!PBASBBPCONBB.PCONBB,dPDMFTPDMFPLOCB'PLOCB#\PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPLTySPACAl SPACSPACAl SVER4.0SVER K<SVER,,/Scal@Scan@TUBEH11Tmpr<UserphARphCHphDYbigphQLcphTRphTR