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  $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 300f28bddeb24579a328c67248d4658fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-Well!K1320_F11_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z )7ERZ_``acgklje^WROOQSSOF9) 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" $&  "$&$) /(!!!..#"*$'05: ,) 3 (*(!,, !-()! $+"26()78-*//+373(+$8.&"13D/*:A3;:8:RGB0F-K>E@;80H;L2@<A.FI5.UH<)(?BXTEDE?FLGXRFEMMLPASZagT?HnHE^RA:KIBRBDIOb_EFLME^YOXUZveXSZ[PUaEYP]\Wae[JpsU_swmnrdRxifmg|uyY{v{cYn|h[kkpxxk[ujy{hno~]lfivwhip ~~~#~~~~~~~~~o~~~~~~::Xd&(-88p Ip GdG^,Dp-pFbip;ALd0{J+8d88sM3Ml0H4 OX C(wt,4ANp|8:p?V.L*1M#pppYpFOd8Z 8&ipPp,pp#pp_.ppd+f1okG$l%]c.6t+U+TV-%Hh$0I9/y"1d/Fn#{6J0$E2$3-a_#&leI$ ZCM0 )"A*/?& < ,H1!5X(Uh5A!#4%%(#F7mA9-#/,mh-7U95GM">)G///O)/KP31.2&$ /AU'?@';&7)8Q',:7F@"&!B*0Z>@1,JW69+$& ;$(FDA40/ #=5"(5)'!J-Q=#"'FZf=N,_VM5)9$=1mK:MK@#?GJ8&R>3$1O`J+>W?$/,03<-%\'78:->5,Bf-P_2$E`:)U9J#I`',-I:}Cy39<HFptN/:*W<y?Ca|?)?Z FG11@fd]InYZ1'CC>_NokY@*)DWy:48X]6hxm10GO|N^EZOpRm}]Q[ w{v:TGH1Q"jrFIF24{5w|4a+;Z=[\)awX3!DLQb|`Sfdm$pck|Fy N4_}h *CGAAAAAAATAGATCTATAGCAGAGCAATCGAATATCATTACTAAATTTGAATTACTACACCGAACGACACAACACCAACATTCATGCACGCAATAGGGGTAACGGCCCATACAGAGTGACGCCCAAAGAAGGGAGGAACGGTGGTAGGTCGCAATACTCTTCCGGGATACAGGAAGAGATCACGGACCTAGGACCATACGGGCTTAGCTTGTACCGAAGGCCGGCGCGAGAGAATCAGCCCTTCCTCCACGGGGAAAAATTGAAAAGGCGCCTTAGGAGGCATACCCTTAAAATCCTCTGGAAGCAGTCTCGTTACTACAGCGTCTTCTTCCGTAGGAAGGAGGACTCTTAGGACTACAGCCCTACCTTGCCCTATTTGTAGAAGAAGAGGATACTCCGGATGCAGCGCCGATTGCTCCTACGGATGCTTTTCTTGATGGCGAAGAGACAATAGGTACGGCGACGATATAAGAATATTCGTTGCCTCATAGAGCAGAATATCCCGAATTTAGATATTATGCATCACAACCTCGGTGTATACCACTAGCTCAATACTCTGTGAAGTTTTGTACATAACCAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:39:11 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt|ASPt|B1Pt{B1Pt{BCTS^BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ;wJTDATA ;wJDATA ;wJCODATA ;wJDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~]=LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS1P1AMEHP1RLEDFP1WDEMZP2AMEVnP2BAEEZP2RLEQPBASEE4cPBASEE2PCONEEBPCONEE?PDMFTPDMF2PLOCE;2PLOCE6PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%1SCAN@SMED SMLtSMPL""TySPACAlv8SPAC1SPACAlv8SVER4.0SVER ^SVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCH2phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt|ASPt|B1Pt{B1Pt{BCTS^BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ;wJTDATA ;wJDATA ;wJCODATA ;wJDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabm!FVocmFWO_GATCFeat//lGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~]=LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS1P1AMEHP1RLEDFP1WDEMZP2AMEVnP2BAEEZP2RLEQPBASEE4cPBASEE2PCONEEBPCONEE?PDMFTPDMF2PLOCE;2PLOCE6PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%1SCAN@SMED SMLtSMPL""TySPACAlv8SPAC1SPACAlv8SVER4.0SVER ^SVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCH2phDYbigphQLcphTRphTRYxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["#87zFl5xKbhh*lC#|6sBg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLVnG!N`!$$RevlKowC#|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#{, #L6q.`tlBEV 6-]3:!h]R_s_beT 1"/"%)O~exg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhI49uu2r6$9_cs:cZa9ZUeFMFu}F_7(gFj?({y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`Vcy[*oCf$`6A&03d02R P,hzp!tY`