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  $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c15f8578a8ca44ec9a63eef3c4efbfecSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-Well 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"2KXD)&- "Z/ 5A6'r%}\c&)*1'!!'% #, 3"$XD'2T0'&TL.*<.#3.$2"96\)()2!B:-6/ B$!-%)%$('+"=*=29'0S"#%3B&/4.k)((&'*702H="* &#,G'3*7,9K#)-^  " 9;4HC$0!:V'EF8,-M>'! % 3` *-H/F$:471!--4:^#(C5#$4(OD!+$)ZEAC !;*F`~2T&EB=T6>646=/)$-"#@)3HL+$PtR+^5DQ:/=z!d+%K08=O"Z=i(PC~94Rc8X+<qLWKu*kZJP8xU8~lW+rDn<3J$J-FowAfng|@LLsiM)r_SFk"+O{_Z}BYJSs\; X77-92-H#CCAACTATCACTCTATAGCATAACCAGCGCATATTATAACCAGAGTTGAATTATTACACAGAACCTTGGAACACCAACATTCCTGGACTTCATATTGGAAACCACCCATACAGCGTGACGCACATCGTTGTTAGGACAAGGATTACCCCAAAGTACTCCCCGGTTATACGGACAGAGAGATTGGAACGCGGACTTAACGGACTCGGTATTCACCAAAGGAGGTCACATAACCATCTCCCCATTCGCCTCGGGGATGTAGGAAATAGCCGCCTCATTGGCTAGACACTTATACTTCTTTACAAGGAATAGCGCTACGCTATAGTATACTTCCGTAGGTCGAACGACACTTGGGAGTACAGCCCTACCCACACCAGTTCAAACAACAATAGGATAATTCGGAGATTACGGAGTTAGTTGCTCCTCACGTTTTTTTTCTAGCTGAACAGATACGCAGTACGCCAACGATATAACAATATTCGTTGCGTTATACTGCAATGTGTTATGACTCTAAATATTATTCATCACATCCGCGATGTATACCACACGCGCGATTCTCTGTGGAGTTTTGTACATAAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:38:30 -04:00AEPt?vAEPt?vAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?&TDATA ?&DATA ?&SDATA ?&,DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~|sLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQZP1AMBh!P1RLBcP1WDBlP2AMBuP2BABBz1P2RLBq)PBASBBSPBASBBQPCONBBa[PCONBB_PDMFTPDMFQqPLOCBZPLOCBVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%QRSCAN@SMED SMLtSMPL!!TySPACAdXSPACQjSPACAdXSVER4.0SVER }SVER,,/Scal@Scan@TUBEE6Tmpr<UserphARphCHQphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt?vAEPt?vAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?&TDATA ?&DATA ?&SDATA ?&,DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabWFVocFFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~|sLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQZP1AMBh!P1RLBcP1WDBlP2AMBuP2BABBz1P2RLBq)PBASBBSPBASBBQPCONBBa[PCONBB_PDMFTPDMFQqPLOCBZPLOCBVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%QRSCAN@SMED SMLtSMPL!!TySPACAdXSPACQjSPACAdXSVER4.0SVER }SVER,,/Scal@Scan@TUBEE6Tmpr<UserphARphCHQphDYbigphQLcphTRphTRKj5)l#O,[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:TS]*|QK:}$OEH3udrVbH`6@m rQ%`)v4A`n\`c6{=m&Hb~9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*I_B]x}rJl6d+lbf*yA=$I;i/cE`\P =h W~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG